1.Protective effect of aminophylline on cerebral injury during cardiopulmonary bypass in infants.
Shan PAN ; Guoqiang LIN ; Haihe JIANG ; Rimao HUANG
Journal of Central South University(Medical Sciences) 2009;34(11):1126-1131
OBJECTIVE:
To investigate the protective effect of aminophylline on cerebral injury induced by cardiopulmonary bypass (CPB) in infants.
METHODS:
Forty patients who underwent ventricular septal defect within 3 years old were randomly divided into 2 groups(20 cases in each group).Aminophylline group:aminophylline (5 mg/kg) was injected slowly via the vein after anesthesia and maintained at a dose of 0.5 mg/(kg.h) until the end of CPB.
CONTROL GROUP:
aminophylline was replaced by Ringer's lactated solution. Samples were obtained at the beginning of CPB (T(1)),the end of CPB (T(2)),6 h (T(3)) and 24 h (T(4)) after the operation to measure S-100 beta protein, NSE, tumor necrosis factor-alpha (TNF-alpha), interleukin-8 (IL-8), and interleukin-10 (IL-10) concentration by ELISA in the 2 groups.
RESULTS:
Compared with the time point immediately before CPB, the S-100beta protein,NSE, TNF-alpha, and IL-8 concentration in the 2 groups began to increase with the start of CPB, reached a climax at the end of CPB (T(2)),decreased gradually 6 h after the termination of CPB(T(3)) and could not restore to the level before CPB at T(4)(24 h after the termination of CPB).IL-10 in the 2 groups both increased after the CPB. At T(2) and T(3), S-100beta protein,NSE, TNF-alpha, and IL-8 concentrations were significantly lower than those in the aminophylline group (P<0.05 or P<0.01), while IL-10 was just the opposite.
CONCLUSION
There is cerebral damage induced by CPB. Aminophylline may play a protective role in cerebral injury by modulating the balance between the pro-inflammatory factor and anti-inflammatory factor to reduce the level of S-100beta protein and NSE during CPB and open cardiac surgeries.
Aminophylline
;
administration & dosage
;
therapeutic use
;
Brain Diseases
;
etiology
;
prevention & control
;
Cardiopulmonary Bypass
;
adverse effects
;
Cardiovascular Surgical Procedures
;
Child, Preschool
;
Female
;
Heart Septal Defects
;
drug therapy
;
surgery
;
Humans
;
Infant
;
Injections, Intravenous
;
Male
;
Nerve Growth Factors
;
blood
;
Neuroprotective Agents
;
therapeutic use
;
S100 Calcium Binding Protein beta Subunit
;
S100 Proteins
;
blood
2.Protective effect of right coronary artery ischemic preconditioning on myocardial ischemia reperfusion injury in rabbit heart.
Jun LI ; Guoqiang LIN ; Rimao HUANG ; Huihui LU ; Zhong YANG ; Wanjun LUO
Journal of Central South University(Medical Sciences) 2016;41(10):1047-1051
To explore the protective effects of right coronary artery ischemic preconditioning and post-conditioning on myocardial ischemia reperfusion injury in rabbit heart.
Methods: A total of 30 rabbits were randomly divided into 4 groups: a control group (n=7), an ischemia reperfusion group (IR group, n=8), an ischemic preconditioning group (IPC group, n=8) and an ischemic post-conditioning group (IPO group, n=7). Venous blood samples were taken at pre-operation, 1 and 6 h post-operation, and the concentration of serum creatine kinase isoenzyme (CK-MB) and cardiac troponin-T (cTn-T) were measured. The infarct area of cardiac muscle was calculated.
Results: Compared with the IR group, the levels of CK-MB and cTn-T at 1 and 6 h post-operation in the IPC group and the IPO group were reduced (all P<0.05). Compared with the IR group, the infarct size in the IPC group and the IPO group was significantly decreased, with significant difference (both P<0.05) .
Conclusion: Right coronary artery ischemic preconditioning and post-conditioning exert significant protective effects on the myocardial ischemia reperfusion injury in New Zealand rabbits.
Animals
;
Coronary Vessels
;
Creatine Kinase, MB Form
;
blood
;
Heart
;
Ischemia
;
Ischemic Postconditioning
;
Ischemic Preconditioning
;
Ischemic Preconditioning, Myocardial
;
Myocardial Infarction
;
etiology
;
pathology
;
physiopathology
;
prevention & control
;
Myocardial Ischemia
;
complications
;
therapy
;
Myocardial Reperfusion Injury
;
prevention & control
;
Myocardium
;
Rabbits
;
Troponin T
;
blood