1.Cordyceps bassiana and Production of Stromata in vitro Showing Beauveria Anamorph in Korea.
Jae Mo SUNG ; Je O LEE ; Richard A HUMBER ; Gi Ho SUNG ; Bhushan SHRESTHA
Mycobiology 2006;34(1):1-6
A Cordyceps species was found with a Beauveria anamorph state on larval insect cadavers on Obong mountains in Gangwon Provinces, Republic of Korea. Cultures from discharged ascospores formed an anamorph identifiable as Beauveria bassiana. This teleomorph-anamorph connection was also confirmed by the in vitro production of fertile ascomata from conidial cultures with morphology like that of field-collected specimen. This is the first report of in vitro production of a teleomorph for any Beauveria species. The Cordyceps species has been conspecified as Cordyceps bassiana, a species described from China with B. bassiana anamorph.
Beauveria*
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Cadaver
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China
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Classification
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Cordyceps*
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Gangwon-do
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Insects
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Korea*
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Republic of Korea
2.Fruit Body Formation on Silkworm by Cordyceps militaris.
In Pyo HONG ; Pil Don KANG ; Ki Young KIM ; Sung Hee NAM ; Man Young LEE ; Yong Soo CHOI ; Nam Suk KIM ; Hye Kyung KIM ; Kwang Gill LEE ; Richard A HUMBER
Mycobiology 2010;38(2):128-132
Injection inoculation protocols for fruit body formation of Cordyceps militaris (C. militaris) were investigated to improve the incidence of infection in the silkworm species Bombyx mori (B. mori). Injection, with suspensions of C. militaris hyphal bodies into living silkworm pupae, was used to test for fruit body production. Use of Daeseungjam rather than Baegokjam or Keumokjam varieties of B. mori is thought to be suitable for infection by C. militaris. From mounting, nine-day-old to 11-day-old pupae showed the best incidence of infection with a 100 microL injection volume. Silkworm pupae injected with a hyphal suspension concentration of more than 2 x 105 colony-forming unit (cfu) recorded a greater than 96% incidence of infection. Also, fruit bodies of C. militaris were induced and produced at a light intensity between 500 and 1,000 lx.
Bombyx
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Cordyceps
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Fruit
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Incidence
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Light
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Pupa
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Stem Cells
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Suspensions