1.DNA vaccine encoding L7/L12-P39 of Brucella abortus induces protective immunity in BALB/c mice.
De-yan LUO ; Peng LI ; Li XING ; Guang-yu ZHAO ; Wei SHI ; Song-le ZHANG ; Xi-liang WANG
Chinese Medical Journal 2006;119(4):331-334
Animals
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Antibodies, Bacterial
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blood
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Bacterial Proteins
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genetics
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immunology
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Brucella Vaccine
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immunology
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Brucella abortus
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immunology
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Female
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Immunization
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Interferon-gamma
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biosynthesis
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Lymphocyte Activation
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Mice
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Mice, Inbred BALB C
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Periplasmic Binding Proteins
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genetics
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immunology
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Ribosomal Proteins
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genetics
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immunology
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Vaccines, DNA
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immunology
2.Molecular cloning of ribosomal P protein in Toxoplasma gondii and the availability to detect antibody against recombinant protein in toxoplasmosis patients.
Hye Jin AHN ; Sera KIM ; Ho Woo NAM
The Korean Journal of Parasitology 2003;41(2):89-96
Among the panel of monoclonal antibodies (mAb) against Toxoplasma gondii, mAb of Tg621 (Tg621) clone blotted 38 kDa protein which localized in the cytoplasm of tachyzoites by immunofluorescence microscopy. The protein was not released into the parasitophorous vacuole during or after invasion. The cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg621. The full length cDNA sequence was completed with 5'-RACE as 1, 592 bp, which contained open reading frame of 942 bp. The deduced amino acid sequence of Tg621 consisted of a polypeptide of 313 amino acids, with significant homology to ribosomal P proteins (RPP) of other organisms especially high to those of apicomplexan species. The expressed and purified TgRPP was assayed in western blot with the sera of toxoplasmosis patients and normal sera, which resulted in the 74.0% of positive reactions in toxoplasmosis patients whereas 8.3% in normal group. Therefore, the antibody formation against TgRPP in toxoplasmosis patients was regarded as specific for T. gondii infection and suggested a potential autoantibody.
Amino Acid Sequence
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Animals
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Antibodies, Monoclonal/immunology
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Antigens, Protozoan/immunology
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Base Sequence
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Cloning, Molecular
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DNA, Protozoan/chemistry/genetics
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Human
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Molecular Sequence Data
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Protozoan Proteins/*genetics/immunology
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Recombinant Proteins/immunology
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Ribosomal Proteins/*genetics/immunology
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Sequence Alignment
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Support, Non-U.S. Gov't
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Toxoplasma/*genetics
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Toxoplasmosis/blood/*parasitology
3.Isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an Ehrlichia canis strain from southeastern Brazil.
Rosiane Nascimento ALVES ; Susana Elisa RIECK ; Carlos UEIRA-VIEIRA ; Marcelo Bahia LABRUNA ; Marcelo Emilio BELETTI
Journal of Veterinary Science 2014;15(2):241-248
Amplification of the 16S rRNA gene from a blood sample obtained from a dog in southeastern Brazil was used to confirm a naturally acquired Ehrlichia (E.) canis infection. Following isolation and culturing of the new bacterial strain called Uberlandia, partial sequences of the dsb and p28 genes were obtained. The dsb partial sequence of the novel strain was 100% similar to dsb gene sequences of E. canis obtained from different geographic areas around the world. Conversely, the p28 partial sequence for the E. canis Uberlandia strain differed at several nucleotides from other sequences available in GenBank. To confirm the antigenic profile of the Uberlandia strain, an indirect immunofluorescence assay against E. canis antigens was performed using dog sera collected from two different areas in Brazil (Uberlandia and Sao Paulo). The results suggest that both antigens were able to identify animals seropositive for E. canis in Brazil since these Brazilian strains appear to be highly conserved.
Animals
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Antigens, Bacterial/blood/*diagnostic use
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Bacterial Outer Membrane Proteins/genetics/metabolism
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Bacterial Proteins/*genetics/metabolism
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Base Sequence
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Brazil
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Dog Diseases/diagnosis/*microbiology
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Dogs
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Ehrlichia canis/*genetics/*immunology/isolation & purification
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Ehrlichiosis/diagnosis/microbiology/*veterinary
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Fluorescent Antibody Technique, Indirect/veterinary
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Male
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Molecular Sequence Data
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Polymerase Chain Reaction/veterinary
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RNA, Ribosomal, 16S/genetics/metabolism
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Sequence Alignment/veterinary
4.An Autochthonous Case of Canine Visceral Leishmaniasis in Korea.
Dong Ha BHANG ; Ul Soo CHOI ; Hyun Jeong KIM ; Kyoung Oh CHO ; Sung Shik SHIN ; Hee Jeong YOUN ; Cheol Yong HWANG ; Hwa Young YOUN
The Korean Journal of Parasitology 2013;51(5):545-549
A 12-year-old spayed female mixed-bred dog presented with nasal bleeding of 2 days duration and a skin nodule in the left flank. No abnormalities were found in coagulation profiles and blood pressure. Cytological evaluation of the nodule revealed numerous characteristic round organisms having a nucleus and a bar within macrophages and in the background, consistent with leishmaniasis. In vitro culture was unsuccessful but PCR of the nodular aspirate identified the organisms as Leishmania infantum, and the final diagnosis was canine leishmaniasis. No history of travel to endemic countries was noted. Because the dog had received a blood transfusion 2 years before the illness, serological screening tests were performed in all donor dogs of the commercial blood bank using the commercial Leishmania ELISA test kit, and there were no positive results. Additional 113 dogs with hyperglobulinemia from Seoul were also screened with the same kits but no positive results were obtained. To the best of the author's knowledge this is the first autochthonous case of canine leishmaniasis in Korea.
Animals
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Base Sequence
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DNA, Protozoan/chemistry/genetics
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DNA, Ribosomal/chemistry/genetics
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Dog Diseases/*diagnosis/epidemiology
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Dogs
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Enzyme-Linked Immunosorbent Assay/veterinary
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Female
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Giant Cells/pathology
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Leishmania infantum/genetics/immunology/*isolation & purification
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Leishmaniasis, Visceral/diagnosis/*veterinary
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Molecular Sequence Data
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Polymerase Chain Reaction/veterinary
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Protozoan Proteins/genetics
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Republic of Korea
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Sequence Analysis, DNA/veterinary
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Serologic Tests/veterinary