1.Preparation and antioxidant activity detection of collagen peptide from Cirrhinus molitorella skin.
Cuiling WU ; Ribang WU ; Dan LIU ; Xinghao YANG ; Jiang ZHANG ; Jiafeng HUANG ; Hailun HE
Chinese Journal of Biotechnology 2016;32(12):1727-1734
In order to prepare antioxidant peptide through hydrolyzing low-value protein resources with bacterial extracellular proteases and to discover novel proteases, crude extracellular protease from Pseudoalteromonas sp. SHK1-2 was obtained through fermentation which was used to hydrolyze collagen extracted from Cirrhinus molitorella skin. Small peptide fraction was isolated from hydrolysate by ultrafiltration and Sephadex LH-20 size exclusion chromatography and showed 1, 1-diphenyl-2-picrylhydrazyl radical scavenging activity (35.6%±7%), oxygen radical absorbance capacity and inhibition of DNA oxidation damage. The molecule weight was 776.2 Da, and amino acid sequence was Thr-Ala-Gly-His-Pro- Gly-Thr-His through liquid chromatography mass spectrum. Our findings suggest that peptide obtained from low-value protein of fish waste by hydrolysis with bacterial protease has antioxidant activity.
Amino Acid Sequence
;
Animals
;
Antioxidants
;
chemistry
;
Chromatography, Gel
;
Collagen
;
chemistry
;
Cyprinidae
;
Dextrans
;
Hydrolysis
;
Oxidation-Reduction
;
Peptide Hydrolases
;
Peptides
;
chemistry
;
Skin
;
chemistry