Aims: Native rhizobia from root nodules of mungbean could reduce atmospheric nitrogen to ammonia for assimilation. The objective of this study was to find the best native rhizobium from mungbean. Methodology and results: Three rhizobia isolates from three mungbean varieties (Maejo 3, Khampangsan 2 and Chainat 72) were collected from 10 undamaged fresh nodules at Prince Chakrabandh Pensiri Center for Plant Development, Saraburi Province, Thailand in 2016. 16S rDNA analysis identified the three rhizobia isolates as Bradyrhizobium sp. (SB1), Bradyrhizobium elkanii (SB2) and Rhizobium sp. (SB3). All the isolates could grow well in yeast mannitol agar (YMA) at pH 7, and all isolates could tolerate up to 35 °C, with isolate SB3 tolerate up to 45 °C. Isolate SB2 produced the highest amount of indole acetic acid (IAA; 8.37 mg/L) and had the highest phosphate solubilization index (7.60 SI). In a Leonard jar trial, inoculation with isolate SB2 resulted in the highest shoot fresh and dry biomass of mungbean host. Further, the mungbean inoculated with SB2 had the highest number of root nodules, nodule fresh dry weight, chlorophyll content index, and shoot and root nitrogen contents. Conclusion, significance and impact of study This study suggested that the strain SB2 (B. elkanii) is a suitable bioinoculant to improve mungbean growth and yield.
Vigna--microbiology ; Rhizobiaceae

Rhizobium species, aerobic Gram-negative rods found in soils worldwide, are well-known tumor-inducing pathogens in plants. Since 1980, when the first case of prosthetic valve endocarditis caused by Rhizobium radiobacter was reported, R. radiobacter has been recognized as an opportunistic human pathogen. In Korea, three cases of infection by this organism have been reported. Recently, we experienced a case of R. radiobacter bacteremia in a patient who underwent chemotherapy for lymphoma. Here, we report the case with a review of the literature.
Agrobacterium tumefaciens ; Bacteremia ; Endocarditis ; Humans ; Korea ; Lymphoma ; Rhizobium ; Soil

Heavy metal pollution of soil is a significant environmental problem and has its negative impact on human health and agriculture. Rhizosphere, as an important interface of soil and plant, plays a significant role in phytoremediation of contaminated soil by heavy metals, in which, microbial populations are known to affect heavy metal mobility and availability to the plant through release of chelating agents, acidification, phosphate solubilization and redox changes, and therefore, have potential to enhance phytoremediation processes. Phytoremediation strategies with appropriate heavy metal-adapted rhizobacteria have received more and more attention. This article paper reviews some recent advances in effect and significance of rhizobacteria in phytoremediation of heavy metal contaminated soils. There is also a need to improve our understanding of the mechanisms involved in the transfer and mobilization of heavy metals by rhizobacteria and to conduct research on the selection of microbial isolates from rhizosphere of plants growing on heavy metal contaminated soils for specific restoration programmes.
Biodegradation, Environmental ; Biological Availability ; Metals, Heavy ; metabolism ; Plant Development ; Plants ; metabolism ; microbiology ; Rhizobiaceae ; metabolism ; Soil Microbiology ; Soil Pollutants ; metabolism

Rhizobium radiobacter has been recognized as a rare pathogen affecting debilitated patients and usually associated with indwelling foreign body. Rhizobium radiobacter is a rare pathogen of peritonitis in patients on peritoneal dialysis. It is assumed that a cure can hardly be expected without removal of a peritoneal dialysis catheter. We experienced a case of Rhizoboum radiobacter peritonitis in a patient on CAPD successfully cured without removal of the peritoneal dialysis catheter. The patient was 42-year-old male and maintained on CAPD for 2 months. He visited with cloudy peritoneal dialysate effluent and showed mild abdominal tenderness. 650 leucocytes/microL were counted and Rhizobium radiobacter was isolated in the peritoneal dialysate effluent. His peritonitis was completely resolved with 3 weeks course of intraperitoneal ceftazidime and oral ciprofloxacin. He has maintained on CAPD without recurrence of peritonitis for 12 months.
Adult ; Agrobacterium tumefaciens* ; Catheters* ; Ceftazidime ; Ciprofloxacin ; Foreign Bodies ; Humans ; Male ; Peritoneal Dialysis ; Peritoneal Dialysis, Continuous Ambulatory* ; Peritonitis* ; Recurrence ; Rhizobium*

Rhizobium radiobacter, which has been previously discribed as Agrobacterium radiobacter, is a group of phytopathogenic organisms widely distributed in soil. Over the past decade, increasing number of infections due to Rhizobium radiobacter has been reported. Rhizobium radiobacter is now recognized as rare human pathogens affecting mostly immunocompromised hosts and is an opportunistic pathogen often associated with indwelling catheters. We report a case of bacteremia due to Rhizobium radiobacter in an acquired immunodeficiency syndrome (AIDS) patient. The patient was admitted for fever. In the blood culture, Rhizobium radiobacter was isolated. These symptoms and signs were successfully resolved with antibiotics.
Acquired Immunodeficiency Syndrome* ; Agrobacterium tumefaciens* ; Anti-Bacterial Agents ; Bacteremia* ; Catheters, Indwelling ; Fever ; Humans ; Immunocompromised Host ; Rhizobium* ; Soil

Rhizobium radiobacter, which has been previously discribed as Agrobacterium radiobacter, is a group of phytopathogenic organisms widely distributed in soil. Over the past decade, increasing number of infections due to Rhizobium radiobacter has been reported. Rhizobium radiobacter is now recognized as rare human pathogens affecting mostly immunocompromised hosts and is an opportunistic pathogen often associated with indwelling catheters. We report a case of bacteremia due to Rhizobium radiobacter in an acquired immunodeficiency syndrome (AIDS) patient. The patient was admitted for fever. In the blood culture, Rhizobium radiobacter was isolated. These symptoms and signs were successfully resolved with antibiotics.
Acquired Immunodeficiency Syndrome* ; Agrobacterium tumefaciens* ; Anti-Bacterial Agents ; Bacteremia* ; Catheters, Indwelling ; Fever ; Humans ; Immunocompromised Host ; Rhizobium* ; Soil

Fungi of the Metarhizium genus are a very versatile model for understanding pathogenicity in insects and their symbiotic relationship with plants. To establish a co-transformation system for the transformation of multiple M. robertsii genes using Agrobacterium tumefaciens, we evaluated whether the antibiotic nourseothricin has the same marker selection efficiency as phosphinothricin using separate vectors. Subsequently, in the two vectors containing the nourseothricin and phosphinothricin resistance cassettes were inserted eGFP and mCherry expression cassettes, respectively. These new vectors were then introduced independently into A. tumefaciens and used to transform M. robertsii either in independent events or in one single co-transformation event using an equimolar mixture of A. tumefaciens cultures. The number of transformants obtained by co-transformation was similar to that obtained by the individual transformation events. This method provides an additional strategy for the simultaneous insertion of multiple genes into M. robertsii.
Agrobacterium ; Agrobacterium tumefaciens ; Fungi ; Insects ; Metarhizium* ; Methods ; Streptothricins ; Virulence

Actin filaments play an important role in fungal life processes such as growth, development and cytokinesis. The expression vector pSULPH-Lifeact-mCherry of fluorescent mCherry-labeled actin was transferred into Verticillium dahliae Kleb. wild type V592 by the genetic transformation system mediated by Agrobacterium tumefaciens to obtain the stable fluorescent labeled actin strain V592/Lifeact-mCherry. Then we detected its biological phenotype and the dynamic changes of actin fluorescence during the process of spore germination, mycelial growth and development. There was no significant difference in the colony morphology, colonial growth rate, sporulation and germination rate between the fluorescent labeled actin strain and the wild type. The actin fluorescence signal was observed at the tip of the conidia and hyphae and the septum clearly. Actin participated in the formation of the contractile actomyosin ring (CAR) during cytokinesis by observing the dynamic behavior of the actin in the process of hyphal septum formation. The fluorescent labeled actin strain can be used to study the dynamics of actin in fungal development to provide theoretical and practical support for further study of the mechanism of actin in fungal development and pathogenesis.
Actins ; Agrobacterium tumefaciens ; Plant Diseases ; Spores, Fungal ; Verticillium

A mutant library of Cordyceps militaris was constructed by improved Agrobacterium tumefaciens-mediated transformation and screened for degradation features. Six mutants with altered characters in in vitro and in vivo fruiting body production, and cordycepin formation were found to contain a single copy T-DNA. T-DNA flanking sequences of these mutants were identified by thermal asymmetric interlaced-PCR approach. ATP-dependent helicase, cytochrome oxidase subunit I and ubiquitin-like activating enzyme were involved in in vitro fruiting body production, serine/threonine phosphatase involved in in vivo fruiting body production, while glucose-methanol-choline oxidoreductase and telomerase reverse transcriptase involved in cordycepin formation. These genes were analyzed by bioinformatics methods, and their molecular function and biology process were speculated by Gene Ontology (GO) analysis. The results provided useful information for the control of culture degeneration in commercial production of C. militaris.
Agrobacterium ; Agrobacterium tumefaciens ; Biology ; Computational Biology ; Cordyceps* ; Electron Transport Complex IV ; Fruit* ; Fungi* ; Gene Ontology ; Telomerase

To develop a genetic transformation method of Aureobasidium pullulans and T-DNA insertion for high-efficient screening of polymalic acid (PMA) producing strain. Agrobacterium tumefaciens-AGL1, containing the selection genes encoding hygromycin B phosphotase or phosphinothricin acetyltranferase, was used to transform Aureobasidium pullulans CCTCC M2012223 and transformants were confirmed by colony PCR method. Transferred DNA (T-DNA) insertional mutants were cultured in microwell plate, and screened for high-titer PMA producing strain according to the pH response model. DNA walking was used to detect the insertion sites in the mutant. Results show that the selection markers could stably generated in the transformants, and 80 to 120 transformants could be found per 10(7) single cells. A high-titer PMA mutant H27 was obtained, giving a good PMA production caused by the disruption of phosphoglycerate mutase, that increased by 24.5% compared with the control. Agrobacterium tumefaciens-mediated transformation and high-efficient screening method were successfully developed, which will be helpful for genetic transformation of Aureobasidium pullulans and its functional genes discovery.
Agrobacterium tumefaciens ; Ascomycota ; genetics ; metabolism ; DNA, Bacterial ; Malates ; metabolism ; Polymerase Chain Reaction ; Polymers ; metabolism ; Transformation, Genetic