OBJECTIVETo investigate KiA10 expression in colorectal cancer and related colorectal tissues.

METHODSKiA10 expression was detected by immunohistochemistry in tissue specimens from 101 cases of colorectal cancer and matched cut-margin mucosa and mucosa adjacent to cancers; also in colorectal specimens from 16 cases of ulcerative colitis, 16 of Crohn's disease and 3 healthy subjects.

RESULTThe positive rate of KiA10 expression in colorectal cancer was 14.85% (15/101); KiA10 positive crypts was demonstrated in 18 cut-margin mucosa and 18 mucosa adjacent to tumors respectively, but they were not matched with each other.

CONCLUSIONThe results suggest that KiA10-positive crypts of the colon might be a preneoplastic lesion.

Adult ; Aged ; Aged, 80 and over ; Antigens, Nuclear ; analysis ; Biomarkers, Tumor ; analysis ; Colorectal Neoplasms ; pathology ; Female ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Precancerous Conditions ; pathology

OBJECTIVE: To investigate the specificity and sensitivity of the PCR technique in the identification of bcl-1/IgH gene rearrangement in mantle cell lymphoma (MCL) and to characterize the bcl-1/IgH junction DNA sequences. METHODS: A semi-nested PCR method to amplify bcl-1/IgH gene rearrangement in DNA from fresh frozen lymphonode specimen was established. Twenty-eight cases of mantle cell lymphoma were analyzed for the presence of bcl-1/IgH gene rearrangement. The rearrangement products was cloned and sequenced to recognize the junction sequences, the breakpoints in the bcl-1 region, and J(H) gene involved in the rearrangement. RESULTS: A bcl-1/IgH gene rearrangement was detected in 17 out of 28 cases of MCL, while only 9 cases was detected with single step PCR method (X(2)=4.59, P<0.05). The rearrangement product varied in size between 74 to 162 base pairs, and the length of the junction sequences ranged from 6 to 24 base pairs. Ten different bcl-1 breakpoints were clustered within 65 base-pair spans, among which, 5 breakpoints (located at 430, 440, 451, 486 and 492) were never reported by other authors. The most common J(H) gene segments utilized in the translocation were J(H) 4 (8/18), then J(H)5 (3/18), J(H)6 (2/18), J(H)4/5 (2/18). J(H)1 2/18, and J(H)3 (1/18). CONCLUSION: These results indicate that the semi-nested PCR is a specific and sensitive method for the detection of bcl-1/IgH gene rearrangement in mantle cell lymphoma, which has implications for both the diagnosis and clinical management of mantle cell lymphoma. The recognization the potential mechanism of bcl-1/IgH gene rearrangement will help us to know the exact pathogenic machanisms of MCL.