1.Single-cell profiling reveals Müller glia coordinate retinal intercellular communication during light/dark adaptation via thyroid hormone signaling.
Min WEI ; Yanping SUN ; Shouzhen LI ; Yunuo CHEN ; Longfei LI ; Minghao FANG ; Ronghua SHI ; Dali TONG ; Jutao CHEN ; Yuqian MA ; Kun QU ; Mei ZHANG ; Tian XUE
Protein & Cell 2023;14(8):603-617
Light adaptation enables the vertebrate visual system to operate over a wide range of ambient illumination. Regulation of phototransduction in photoreceptors is considered a major mechanism underlying light adaptation. However, various types of neurons and glial cells exist in the retina, and whether and how all retinal cells interact to adapt to light/dark conditions at the cellular and molecular levels requires systematic investigation. Therefore, we utilized single-cell RNA sequencing to dissect retinal cell-type-specific transcriptomes during light/dark adaptation in mice. The results demonstrated that, in addition to photoreceptors, other retinal cell types also showed dynamic molecular changes and specifically enriched signaling pathways under light/dark adaptation. Importantly, Müller glial cells (MGs) were identified as hub cells for intercellular interactions, displaying complex cell‒cell communication with other retinal cells. Furthermore, light increased the transcription of the deiodinase Dio2 in MGs, which converted thyroxine (T4) to active triiodothyronine (T3). Subsequently, light increased T3 levels and regulated mitochondrial respiration in retinal cells in response to light conditions. As cones specifically express the thyroid hormone receptor Thrb, they responded to the increase in T3 by adjusting light responsiveness. Loss of the expression of Dio2 specifically in MGs decreased the light responsive ability of cones. These results suggest that retinal cells display global transcriptional changes under light/dark adaptation and that MGs coordinate intercellular communication during light/dark adaptation via thyroid hormone signaling.
Animals
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Mice
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Dark Adaptation
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Light
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Retina
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Retinal Cone Photoreceptor Cells/metabolism*
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Adaptation, Ocular
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Neuroglia/physiology*
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Cell Communication
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Thyroid Hormones
2.Postsynaptic calcium pathway contributes to synaptic plasticity between retinal cones and luminosity-type horizontal cells.
Shi-Yong HUANG ; Jian-Feng HU ; Hai-Qing GONG ; Pei-Ji LIANG
Acta Physiologica Sinica 2006;58(5):407-414
It was previously found that the efficacy of synaptic transmission between retinal cone systems and luminosity-type horizontal cells (LHCs) was activity-dependent. Repetitive activation of red-cone pathway increased the LHCos hyperpolarizing response to red light, and the response enhancement was reversible. In this study, intracellular recording and pharmacological method were applied to investigate the mechanism(s) underlying red-flickering-induced response enhancement. Lowering intracellular Ca(2+) in the LHC by intracellular injection of Ca(2+) chelator EGTA prevented the development of red-flickering-induced response enhancement, which implicates the importance of postsynaptic calcium signal. The response enhancement could also be eliminated by a potent antagonist of Ca(2+)-permeable AMPA receptor (CP-AMPAR), which suggests the possibility that Ca(2+) influx via glutamate-gated calcium channels is related to the changes of [Ca(2+)](i). Furthermore, the administration of ryanodine or caffeine also attenuated the phenomenon, which gives evidence that the local calcium signal caused by intracellular calcium-induced calcium release (CICR) may be involved. Taken together, our data implicate that postsynaptic CICR and CP-AMPAR are related to the activity-dependent response enhancement.
Animals
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Caffeine
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pharmacology
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Calcium
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metabolism
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Carps
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Neuronal Plasticity
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physiology
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Receptors, AMPA
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physiology
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Retina
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cytology
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Retinal Cone Photoreceptor Cells
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physiology
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Ryanodine
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pharmacology
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Ryanodine Receptor Calcium Release Channel
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physiology
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Signal Transduction
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physiology
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Synapses
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physiology