The study was carried out to investigate the efficacy of subconjunctivally injected mitomycin-C (MMC) on glaucoma filtration surgery(GFS) in rabbit eyes. Each of 0.05mg, 0.1mg and 0.2mg MMC were injected subconjunctivally into the rabbit eye according to groups I, II, III respectively following GFS. The IOP were returned to preoperative level at postoperative 2 weeks in control group, and at postoperative 12 weeks in group I and II. Histologically the fibrotic reaction appears less intensively in the order of group III, II, I and control group at 2 weeks after GFS. At postoperative 12th week, fibroblasts are more frequently oberved in group I and II than control group. But the proliferation of collagenous and reticulin fibers are less in group I and II than control group. The least amount of fibroblasts, collagenous and reticulin fibers are found in group III. It is suggested that subconjunctival injection of lower concentration of MMC would be effective approval as an adjunctive method to improve more success rate of GFS from this study.
Collagen ; Fibroblasts ; Filtering Surgery* ; Filtration* ; Glaucoma* ; Mitomycin* ; Rabbits* ; Reticulin

The authors studied the retinal vascular patterns of human eye suffered from complicated endophthalmitis following by perforating injury and corneal ulcer. The technique of preparing slides to study the retinal vascular patterns were same as previously noted Part I report. A lot of microorganisms, such as cocci, bacilli and fungi are found in the inflamed foci(with or without filaments) around the retinal vessels unless the vessels were completely occluded. Focal and diffuse exudative vasculitis is related to the species of microorganisms and cbserved stage but almost all retinal vessel waIls show diffuse loss of perivascular argyrophilic fibers, socalled "moth eaten" appearance. In the later stage of vasculitis microorganisms are not found in the inflamed focus("punched out" lesion) of the occluded vessels which show as "bark-stripped-dead tree" appearance in reticulin stain.
Corneal Ulcer* ; Endophthalmitis* ; Fungi ; Humans ; Reticulin ; Retinal Vessels ; Retinaldehyde* ; Vasculitis

Penetrating keratoplasty was performed in six eyes of granular corneal dystrophy. Vision was improved from worse than 0.05 to better than 0.4. Mean follow-up period is 14 months (12-18 months). Corneal deposits were stained for H and E. Masson trichrome, Wilder's reticulin and Luxol fast blue but not for PAS, Congo red and Oil red a stain. Electron micorscopic examination showed that polymorphic, electron dense rod-shaped bodies were present in the Bowman's layer and between stromal lamellae and in the interceliular space of corneal epithelium. And, for the first time we found a area looked like a transformation from normal stroma to the granular deposit.
Congo Red ; Epithelium, Corneal ; Follow-Up Studies ; Keratoplasty, Penetrating ; Reticulin

A solitary cutaneous myxoma, referred to as superficial angiomyxomas, is relatively uncommon benign myxoid soft tissue tumors. The lesion usually present on the head, neck, and trunk of adults as a slowly growing asymptomatic nodule. Herein, we describe a case of a pedunculated solitary cutaneous myxoma arising on the vertex of a 48-year-old man, but with no evidence of Carney syndrome. Histologically, the tumor was composed of vascular, mucinous matrix with a network of fine collagen and reticulin fibers, containing stellate and spindle-shaped fibroblasts. There was no evidence of malignancy
Adult ; Carney Complex ; Collagen ; Fibroblasts ; Head ; Humans ; Middle Aged ; Mucins ; Myxoma* ; Neck ; Reticulin

It is very important to be aware of occurrence and distinctive histologic features of gastric glomus tumor because its clinical presentation and endoscopic ficdings are indistinguishable from other gastric tumors. A 58-year-old man was admitted because of intermittent epigastric pain and indigestion for 2 months. Physical examination and laboratory findings revealed within normal limits. Upper gastroendoscopy demonstrated a submucosal mass near the antrum with central mucosal depressionand peripheral multiple erosions. Endoscopic ultrasonography revealed a circumscribed mass that continued to the third and fourth layers which demonstrated a heterogeneous low echo pattern mixed with internal high echo spots. Histologically, the tumor cells have uniform small nuclei with inconspicuous nucleoli. Immunochemical analysis on the tumor cells expressed alpha 1 smooth muscle actin and the tumor cells are enveloped by reticulin fiber, individually or grouped. A case of glomus tumor of the stomach is herein reported with a review of literature.
Actins ; Dyspepsia ; Endosonography ; Glomus Tumor* ; Humans ; Middle Aged ; Muscle, Smooth ; Physical Examination ; Reticulin ; Stomach*

PURPOSE: To evaluate on the basis of histopathologic carrelation the MR findings of mature brain abscess inthe rabbit, with particular attention to rim-enhancing lesions. MATERIALS AND METHODS: The evolution of abscess formation was obtained by the direct inoculation of Staphylococcus aureus into the gray-white matter junctions ofthe brains of 16 rabbits. The stages of brain abscesses were divided into four : early cerebritis (days 1 to 5after inoculation of the organism) ; late cerebritis (days 6 to 14) ; early capsular (days 16 to 21) ; and latecapsular (days 22 to 28). The available MR images showed 14 cases at the stage of early cerebritis, seven at thelate cerebritis stage, three at the early capsular, and one at the late capsular stage. According to the known pathology of brain abscesses and on the basis of both MR imaging and histopathologic findings, the lesions weregrouped according to whether they were found in the central necrotic, border, or peripheral zone. We analyzed the patterns of rim-enhancement (completeness of the rim, thickness, and margin) and the signal intensities of theabscess walls on MR images at each stage. Histopathologic correlation was performed in one case of each stage. We evaluated the presence or absence and degree of infiltration by inflammatory granulation tissue, microhemorrhage, reticulin, collagen, and hemosiderin of the abscess walls. RESULTS: Rim-enhancing lesions were present in threeof 14 cases at the late cerebritis stage, in all three cases at the early capsular, in one at the late capsular, but in none at the early cerebritis stage. The enhancing pattern of the late cerebritis stage was irregular-margined incomplete rim-enhancement, with irregular thickness of the abscess walls (3/3). The enhancing pattern of the capsular stages was well-defined, complete rim-enhancement with uniform thickness of the abscess walls (3/4). The signal intensities of the abscess walls at the late cerebritis and early capsular stages were variable. The late capsular stage was characterized by hypointensity of the abscess wall on both T1- and T2-weighted images. Histopathologically, the capsular stages were distinguished from the late cerebritis stage bythe marked infiltration of reticulin and the presence of collagen in the abscess walls. The most conspicuous pathologic finding distinguishing the late from the early capsular stage was abundant infiltration of the abscess wall by collagen and hemosiderin. CONCLUSION: The enhancing pattern of a brain abscess with mature capsule formation was characterized by a well-defined, complete rim-enhancing abscess wall of uniform thickness. The mature abscess wall was hypointense on both T1- and T2-weighted images, may be explained by marked infiltration bymature collagen and hemosiderin.
Abscess ; Animals ; Brain Abscess* ; Brain* ; Collagen ; Granulation Tissue ; Hemosiderin ; Magnetic Resonance Imaging* ; Rabbits ; Reticulin ; Staphylococcus aureus

Desmoplastic cerebral glioblastoma has been described recently and is a very rare histologic variant of glioblastoma. We report a case of congenital cerebral glioblastoma associated with intense desmoplastic stromal reaction. A male infant was born at 36 gestational weeks by Cesarian section. He had a brain tumor, which was detected by fetal ultrasonography. The tumor was partially resected 2 months after the day of the boy's birth and totally resected when he was one year old. The microscopic features of the tumor were those of glioblastoma, including high cellularity, frequent mitotic figures, vascular endothelial proliferation, and geographic palisading necrosis. The tumor showed an area of intense desmoplasia where tumor cells were surrounded by dense reticulin fibers. The desmoplastic cerebral tumors in children may be a distinct group of brain tumor, and it is important to understand the entity of these tumors which generally seem to be associated with more favorable prognosis compared to other high grade brain tumors.
Brain Neoplasms ; Child ; Glioblastoma* ; Humans ; Infant ; Male ; Necrosis ; Parturition ; Prognosis ; Reticulin ; Ultrasonography, Prenatal

Authors presented the clinical and histopathologic review of 15 cases of retinoblastoma tor last 5 years. Retinal vasculatures in the non-tumorous portion of the retina were observed in 2 cases of retinoblastomas with trypsin digestion and stained with Wilder's reticulin and PAS-Hematoxylin. The following results were obtained. 1. 12 of 15 cases of retinoblastomas were male. 2. 2/3 of the patients had their tumors diagnosed within 2 years, and same numbers of patients operated within 3 years of age. Unusually long period of evolution was noted. 3. Histopathologically, tumors showed necrosis, hemorrhages and calcications, and rosettes were found in 9 cases. The retinoblastoma without rosettes invaded the ocular structures more frequentIy. 4. It suggested to retinal vascular patterns be closely related to onset and degree of differentiation of tumor cells. The retinal vasculatures in the non-tumorous part of retina showed saccular dilatation of the larger vessels associated with pooly differentiated capillary network (polygonal shape) in case of undifferentiated retinoblastoma. Another retinal vasculatures showed fibrous appearence of larger vessels associated with rnicroaneurysmal dilatation of capillaries in case of well differentiated retinoblastoma.
Capillaries ; Digestion ; Dilatation ; Hemorrhage ; Humans ; Male ; Necrosis ; Reticulin ; Retina ; Retinaldehyde ; Retinoblastoma* ; Trypsin

PURPOSE: To describe soft-tissue radiographic and high-resolution CT findings of radiation-induced lung injury of rabbit over time and to correlate them with pathologic findings. MATERIALS AND METHODS: 15 rabbits were irradiated in the right lung with one fraction of 2000 cGy. After 4, 6, 12, 20, 24 weeks, 3 rabbits in each group were sacrificed and soft-tissue radiographs and high-resolution CT of their lung tissue were obtained. Radiological findings were correlated with pathologic findings. RESULTS: On soft- tissue radiogram, radiation pneumonitis shown as consolidation with air-bronchogram occurred in 3 cases after 6 weeks, and in 1 case after 12 weeks of irradiation. In addition, pneumonic consolidation with adjacent pleural contraction was seen in 2 cases after 12 weeks of irradiation. Fibrotic changes indicated by decreased volume occurred after 20 weeks and combined bronchiectatic change and bronchial wall thickening appeared after 20 weeks(N=1), and 24 weeks(N=3). HRCT findings of radiation pneumonitis were homogeneous, increased attenuation after 4 weeks(N=3), 6 and 12 weeks (each N=I), patchy consolidation after 6 and 12 weeks(each N=2), discrete consolidation after 12, 20 and 24 weeks(each N=I) and solid consolidation after 20 and 24 weeks(each N=2). Pathologically radiation pneumonitis and pulmonary congestion were seen after 4 and 6 weeks. After 6 weeks, collagen and reticulin fibers were detected along alveolar wall. Mixed radiation pneumonitis and fibrosis were detected after 12 weeks. 20 weeks after irradiation, fibrosis was well defined in interstitium and in 24 weeks, decreased number of alveoli and thickening of bronchial wall were defined. CONCLUSION: Radiation pneumonitis was provoked 4 weeks after irradiation on rabbit lung and progressed into radiation fibrosis 20 weeks after irradiation on soft-tissue radiographs and high-resolution CT. High-resolution CT is more precise in detecting early radiation pneumonitis and detailed pathologic findings.
Collagen ; Estrogens, Conjugated (USP) ; Fibrosis ; Lung Injury ; Lung* ; Rabbits ; Radiation Pneumonitis ; Reticulin

PURPOSE: The purpose of this study is to evaluate the usefulness of MR imaging in the staging of brain abscesses and to determine the correlations between pathologic and MR findings. MATERIALS AND METHODS: Experimental brain abscesses were induced by direct inoculation of 1ml suspension of l06/ml Streptococcus pneumoniae into the brain parenchyma of ten New Zealand white rabbits. The evolution of abscess formation was divided into four stages, based on pathological criteria: early cerebritis (days 1 to 5), late cerebritis (days 6 to 10), early capsular (days 11 to 15), and late capsular (day 16 and later). The brain abscess of each animal was examined by MR imaging and light microscopy at 3, 8, 13, and 28 days; T1-weighted, T2-weighted and Gd-enhanced images were obtained. The MR images and pathologic findings of 13 pathologically confirmed clinical cases were compared to MR images of the experimental model. RESULTS: In the experimental model, signal intensity of the abscess content was at all stages hypointense on T1-weighted and hyperintense on T2-weighted images. In all ten cases, Gd-enhanced images showed an ill-defined contrast-enhanced lesion at the early cerebritis stage, and in four of seven cases, irregular ring enhancement at the late cerebritis stage. Pathologic specimens at this latter stage revealed prominent vascular proliferation and infiltration of chronic inflammatory cells. Signal intensity of the abscess wall during the capsular stage showed isointense relative brain parenchyma on T1-weighted images and this was hypointense on T2-weighted images. Gd-enhanced images demonstrated smooth ring enhancement of the abscess wall. At the early capsular stage, pathologic specimens revealed a discrete necrotic center surrounded by infiltration of reticulin and some collagen; at the late capsular stage, these specimens showed marked infiltration of collagen. In clinical cases, the signal intensity of abscess content was at all stages hypointenseon T1-weighted and hyperintense on T2-weighted images. Gd-enhanced images demonstrated ill-defined subtle contrast enhancement at the early cerebritis stage and irregular ring enhancement at the late cerebritis stage. In all cases, signal intensity of the abscess wall during the capsular stage was hypointense on T2-weighted images; at this stage, the abscess wall was showed a pattern of smooth ring enhancement. In clinical cases, hypointensity of the abscess wall, as seen on T2-weighted images, and the enhancement pattern of this wall were identical to these findings in the experimental model. CONCLUSION: In an experimental model, correlation between sequential MR findings can be used to predict the stage of a brain abscess; in clinical cases essential indicators are hypointensity and enhancement pattern of the abscess wall, as seen on T2-weighted images. In cases of brain abscess, MRI is a useful diagnostic modality, and in such cases, also helps determine the most suitable treatment.
Abscess ; Animals ; Brain Abscess* ; Brain* ; Collagen ; Magnetic Resonance Imaging* ; Microscopy ; Models, Theoretical* ; Rabbits ; Reticulin ; Streptococcus pneumoniae