The authors studied the retinal vascular patterns of human eye suffered from complicated endophthalmitis following by perforating injury and corneal ulcer. The technique of preparing slides to study the retinal vascular patterns were same as previously noted Part I report. A lot of microorganisms, such as cocci, bacilli and fungi are found in the inflamed foci(with or without filaments) around the retinal vessels unless the vessels were completely occluded. Focal and diffuse exudative vasculitis is related to the species of microorganisms and cbserved stage but almost all retinal vessel waIls show diffuse loss of perivascular argyrophilic fibers, socalled "moth eaten" appearance. In the later stage of vasculitis microorganisms are not found in the inflamed focus("punched out" lesion) of the occluded vessels which show as "bark-stripped-dead tree" appearance in reticulin stain.
Corneal Ulcer* ; Endophthalmitis* ; Fungi ; Humans ; Reticulin ; Retinal Vessels ; Retinaldehyde* ; Vasculitis

The study was carried out to investigate the efficacy of subconjunctivally injected mitomycin-C (MMC) on glaucoma filtration surgery(GFS) in rabbit eyes. Each of 0.05mg, 0.1mg and 0.2mg MMC were injected subconjunctivally into the rabbit eye according to groups I, II, III respectively following GFS. The IOP were returned to preoperative level at postoperative 2 weeks in control group, and at postoperative 12 weeks in group I and II. Histologically the fibrotic reaction appears less intensively in the order of group III, II, I and control group at 2 weeks after GFS. At postoperative 12th week, fibroblasts are more frequently oberved in group I and II than control group. But the proliferation of collagenous and reticulin fibers are less in group I and II than control group. The least amount of fibroblasts, collagenous and reticulin fibers are found in group III. It is suggested that subconjunctival injection of lower concentration of MMC would be effective approval as an adjunctive method to improve more success rate of GFS from this study.
Collagen ; Fibroblasts ; Filtering Surgery* ; Filtration* ; Glaucoma* ; Mitomycin* ; Rabbits* ; Reticulin

Penetrating keratoplasty was performed in six eyes of granular corneal dystrophy. Vision was improved from worse than 0.05 to better than 0.4. Mean follow-up period is 14 months (12-18 months). Corneal deposits were stained for H and E. Masson trichrome, Wilder's reticulin and Luxol fast blue but not for PAS, Congo red and Oil red a stain. Electron micorscopic examination showed that polymorphic, electron dense rod-shaped bodies were present in the Bowman's layer and between stromal lamellae and in the interceliular space of corneal epithelium. And, for the first time we found a area looked like a transformation from normal stroma to the granular deposit.
Congo Red ; Epithelium, Corneal ; Follow-Up Studies ; Keratoplasty, Penetrating ; Reticulin

PURPOSE: To describe soft-tissue radiographic and high-resolution CT findings of radiation-induced lung injury of rabbit over time and to correlate them with pathologic findings. MATERIALS AND METHODS: 15 rabbits were irradiated in the right lung with one fraction of 2000 cGy. After 4, 6, 12, 20, 24 weeks, 3 rabbits in each group were sacrificed and soft-tissue radiographs and high-resolution CT of their lung tissue were obtained. Radiological findings were correlated with pathologic findings. RESULTS: On soft- tissue radiogram, radiation pneumonitis shown as consolidation with air-bronchogram occurred in 3 cases after 6 weeks, and in 1 case after 12 weeks of irradiation. In addition, pneumonic consolidation with adjacent pleural contraction was seen in 2 cases after 12 weeks of irradiation. Fibrotic changes indicated by decreased volume occurred after 20 weeks and combined bronchiectatic change and bronchial wall thickening appeared after 20 weeks(N=1), and 24 weeks(N=3). HRCT findings of radiation pneumonitis were homogeneous, increased attenuation after 4 weeks(N=3), 6 and 12 weeks (each N=I), patchy consolidation after 6 and 12 weeks(each N=2), discrete consolidation after 12, 20 and 24 weeks(each N=I) and solid consolidation after 20 and 24 weeks(each N=2). Pathologically radiation pneumonitis and pulmonary congestion were seen after 4 and 6 weeks. After 6 weeks, collagen and reticulin fibers were detected along alveolar wall. Mixed radiation pneumonitis and fibrosis were detected after 12 weeks. 20 weeks after irradiation, fibrosis was well defined in interstitium and in 24 weeks, decreased number of alveoli and thickening of bronchial wall were defined. CONCLUSION: Radiation pneumonitis was provoked 4 weeks after irradiation on rabbit lung and progressed into radiation fibrosis 20 weeks after irradiation on soft-tissue radiographs and high-resolution CT. High-resolution CT is more precise in detecting early radiation pneumonitis and detailed pathologic findings.
Collagen ; Estrogens, Conjugated (USP) ; Fibrosis ; Lung Injury ; Lung* ; Rabbits ; Radiation Pneumonitis ; Reticulin

Pleomorphic xanthoastrocytoma is known as a rare specialized type of cerebral astrocytoma, which occurs mainly in the temporal loe of the brain in young ages in spite of marked pleomorphism with multinucleated giant cells, lipid-laden xantomatous cells, rich reticulin net-works, and the demonstration of glial fibrillary acidic protein, the prognosis is usually favorable. The authors report three cases of pleomorphic xanthoastrocytoma which were presented with headache and review the past literature.
Astrocytoma ; Brain ; Giant Cells ; Glial Fibrillary Acidic Protein ; Headache ; Prognosis ; Reticulin

The distribution of fibronection was investigated in the carrageenan-induced keratitis of rabbitis. Keratitis was induced by the injection of carrageenan into corneas of the rabbits, and specimens were taken at various intervals. Histological examinations were done with H and E, silver impregnation and PTAH stains. The distribution of fibronectin was observed by using avidin-biotin-peroxydase(ABC) technique. The results were follow. 1. The histological changes in early stages showed acute inflammatory exudate in stroma. After two weeks, granulation tissues were formed. And then after the fourth week, the granulation tissues began to mature with the apperance of fascicle of collagen fibers and thickened epithelium. 2. Fibronectin was demonstrated in a fibrillar appearance in the acute inflammatory exudate of the stroma, which was normally absent. With the development of chronic inflammatory lesions and granulation tissues, the amount of fibronectin was increased, relative to the increase and codistribution of reticular fibers, but with the appearance of mature collagen fibers, the amount was markedly reduced. 3. The fibronectin which was absent in the normal epithelium was prominent in the regenerating epithelium.
Carrageenan ; Collagen ; Coloring Agents ; Cornea ; Epithelium ; Exudates and Transudates ; Fibronectins* ; Granulation Tissue ; Keratitis* ; Rabbits ; Reticulin ; Silver

It is very important to be aware of occurrence and distinctive histologic features of gastric glomus tumor because its clinical presentation and endoscopic ficdings are indistinguishable from other gastric tumors. A 58-year-old man was admitted because of intermittent epigastric pain and indigestion for 2 months. Physical examination and laboratory findings revealed within normal limits. Upper gastroendoscopy demonstrated a submucosal mass near the antrum with central mucosal depressionand peripheral multiple erosions. Endoscopic ultrasonography revealed a circumscribed mass that continued to the third and fourth layers which demonstrated a heterogeneous low echo pattern mixed with internal high echo spots. Histologically, the tumor cells have uniform small nuclei with inconspicuous nucleoli. Immunochemical analysis on the tumor cells expressed alpha 1 smooth muscle actin and the tumor cells are enveloped by reticulin fiber, individually or grouped. A case of glomus tumor of the stomach is herein reported with a review of literature.
Actins ; Dyspepsia ; Endosonography ; Glomus Tumor* ; Humans ; Middle Aged ; Muscle, Smooth ; Physical Examination ; Reticulin ; Stomach*

Generally, the postoperative failure of glaucoma filtrationsurgery(GFS) is caused by scarring of filtering site. Mitomycin C(MMC) has been commonly used for preventing the scarring of filtering site. Though the antifibroblastic effect of this drug is approved clinically, its cytotoxic side effect cannot be overlooked,. alpha-tocopherol is known to inhibit proliferation of fibroblast without cytotoxic complications. In this study, we used alpha-tocopherol in GFS of rabbits and investigated the maintenance of bleb formation, complications and inhibition of fibroblast proliferation compared with MMC. Thirty six rabbits were divided into 6 groups Negative control groups were the balanced salt solution soaked group and the 19% ethanol soaked group and positive control group was soaked with 0.2mg/ml MMC during GFS. As experimental groups, group I, II, III were soaked with 100micrometer, 1mM and 10mM alpha-tocopherol. The results were as follows: For the maintenance of bleb formation, at postoperative 2 weeks, all extinguished in negative control groups and each experimental groups from I to III showed 16.7%, 33.3%, 50%, with highest rate of 66.7% in MMC soaked group, but at 2 months, group III and MMC soaked group showed 33.3% equally. Various complications were present in MMC soaked group but rare in alpha-tocopherol soaked groups. On histologic examinations, proliferation of fibroblast and infiltration of lymphocyte in group II, III and MMC soaked group except group I were much less than those of negative control groups and the degree of inhibition was proportional to concentration of tocopherol. There was no statistic difference(P>0.05) in area of reticulin fibers between group III and MMC soaked group and antifibroblastic effects were also similar between these two groups. These result suggest when directlyconcentration(1mM<) as much as MMC. Moreover, it is expected to be used more safely during GFS withous serious cytotoxic complications. However, application method, dosageand possible long term complications.
alpha-Tocopherol* ; Blister ; Cicatrix ; Ethanol ; Fibroblasts* ; Filtering Surgery* ; Filtration* ; Glaucoma* ; Lymphocytes ; Mitomycin ; Rabbits* ; Reticulin ; Tocopherols

Desmoplastic cerebral glioblastoma has been described recently and is a very rare histologic variant of glioblastoma. We report a case of congenital cerebral glioblastoma associated with intense desmoplastic stromal reaction. A male infant was born at 36 gestational weeks by Cesarian section. He had a brain tumor, which was detected by fetal ultrasonography. The tumor was partially resected 2 months after the day of the boy's birth and totally resected when he was one year old. The microscopic features of the tumor were those of glioblastoma, including high cellularity, frequent mitotic figures, vascular endothelial proliferation, and geographic palisading necrosis. The tumor showed an area of intense desmoplasia where tumor cells were surrounded by dense reticulin fibers. The desmoplastic cerebral tumors in children may be a distinct group of brain tumor, and it is important to understand the entity of these tumors which generally seem to be associated with more favorable prognosis compared to other high grade brain tumors.
Brain Neoplasms ; Child ; Glioblastoma* ; Humans ; Infant ; Male ; Necrosis ; Parturition ; Prognosis ; Reticulin ; Ultrasonography, Prenatal

PURPOSE: We investigated the effect of VEGF usage and the method of surgery on the vascularization rate of the porous orbital implant (Medpor(R)). METHODS: Thirty six Newzealand white rabbits were randomized into 2 groups according to the method of surgery (evisceration and intrascleral implantation and evisceration and retroscleral implantation). Each group was subdivided into two groups, a group treated with VEGF, and the other without VEGF treatment. The degree of vascularization was observed in the four groups at 1, 2, 4 weeks by using cryofilm transfer kit (Finetec, Tokyo, Japan). The implant was sliced at the equator with cryomicrotome and stained with Hematoxylin and Eosin, Masson's trichrome and reticulin. The sample was observed to assay the degree of fibrovascular ingrowth with light microscope. RESULTS: The group in which VEGF was used and the implant was inserted retrosclerally (n=9) showed significantly higher vascularization rate than the other three groups (P<0.05). Vascularization rate of the implant was significantly higher in the group in which VEGF was used (n=18). The rate was 83.6% compared to 64.2% in the control group (P<0.05). Different methods of surgery also showed significant difference. The rate of vascularization was 64.8% in the intrascleral inserted group (n=18) compared to 82.9% in the retroscleral inserted group (P<0.05). However, there was no difference in the vasculariztion rate at different time of observation (p>0.05). CONCLUSIONS: After using VEGF or inserting the implant retrosclerally, the vascularization of the implant was significantly incresed.
Eosine Yellowish-(YS) ; Hematoxylin ; Orbit* ; Orbital Implants* ; Polyethylene* ; Rabbits ; Reticulin ; Vascular Endothelial Growth Factor A*