3.Enteroviruses isolated in patients with acute respiratory infections
Thanh Thi Hien Nguyen ; Van Thi Thanh Trieu
Journal of Preventive Medicine 2007;1(17):10-14
Background: at present, some enterovirus also was considered causes of acute respiratory infections in children. In Vietnam, no studies were published on the enteroviruses causing acute respiratory infections in humans. The research results would contribute to control and prevention of respiratory tract infections. Objectives: to identify enteroviruses causing acute respiratory infection syndrome in children. Subjectives and Method: a descriptive, prospective study, virus isolation. 185 samples of patients with acute respiratory tract infections, collected from both private clinics and from Dak Lak provincial general hospital, in 2004. Cells: RD, Hep2, L20B, and Vero provided by the World Health Organization (WHO). Antisera for diagnosing enteroviruses, antisera for diagnosing adenoviruses: A1-A7. Results: the results showed that 10.8% were positive with enteroviruses. These isolated enteroviruses consist of 13 Coxsackievirus B, 1 Echovirus, 1 Poliosabin type 1, and 5 untyped Enteroviruses. The result also showed that 8.1% of isolated viruses were Adenoviruses. Enteroviruses isolated mainly in September, followed by in February and in July accounted for 60% of isolated viruses. Conclusions: enteroviruses may be one of causes of respiratory infections in children. Some viruses had been isolated in the study such as: Coxsackievirus B, Echovirus, Poliosabin type 1, and untyped Enteroviruses.
Enterovirus/isolation &
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purification
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Enterovirus Infections/virology
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Respiratory Tract Infections/ virology
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4.Research advance in human bocavirus.
Chinese Journal of Contemporary Pediatrics 2010;12(8):678-680
9.Status of enterovirus infection in children with acute lower respiratory tract infection.
Jia LI ; Bing ZHANG ; Ni-Guang XIAO ; Xiao-Fang DING ; Zhi-Ping XIE ; Zhao-Jun DUAN
Chinese Journal of Contemporary Pediatrics 2013;15(5):372-374
OBJECTIVETo investigate the status of enterovirus (EV) infection in children with acute lower respiratory tract infection (ALRTI).
METHODSA total of 404 samples (with odd numbers) of nasopharyngeal aspirates were collected from the children who were hospitalized in the Children's Medical Center, Hunan Provincial People's Hospital due to ALRTI between September 2007 and April 2008. The conserved sequence in the 5'-noncoding region of EV was used to design the primer, and nested RT-PCR was performed to detect EV in the samples.
RESULTSOf the 404 samples, 19 (4.7%) were EV-positive, and mostly taken from children under 3 years of age (95%); there was no significant difference in the detection rate between male and female children. Of the EV-positive children, 13 (68%) were clinically diagnosed with bronchial pneumonia, and 6 (32%) with bronchiolitis; 90% of them showed symptoms of fever, 84% had a cough, 63% had asthma, and 63% had complications mainly including diarrhea (6 cases), granulocytopenia (4 cases), and acute respiratory distress syndrome (2 cases). In addition, 26% of the EV-positive children had leukocyte disorder, more than half had liver dysfunction, and a few had myocardial involvement.
CONCLUSIONSEV is a pathogen that should not be neglected in children with ALRTI. For these children, close attention should be paid to the epidemiological status and clinical features of EV infection, and blood routine examination, liver function test and myocardial enzyme assay should be carried out periodically to improve prognosis.
Acute Disease ; Child ; Child, Preschool ; Enterovirus Infections ; epidemiology ; Female ; Humans ; Infant ; Male ; Nasopharynx ; virology ; Respiratory Tract Infections ; virology
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