1.Airway neutrophils apoptosis in children with severe asthma.
Jing LIAO ; Guang-min NONG ; Min JIANG ; Yu-xian ZHANG ; Xiu-an LIANG ; Jing LIU
Chinese Journal of Pediatrics 2009;47(8):628-631
OBJECTIVETo investigate the changes of neutrophils in airway inflammation in children with severe asthma.
METHODChildren with mild to moderate asthma (n=23), severe asthma (n=16) and healthy control subjects (n=16) underwent lung function tests and sputum induction. The sputum specimens were assayed for cellular differential count, the supernatant and peripheral blood were assayed for the concentrations of IL-8 by "sandwich" enzyme linked immunosorbent assay (ELISA). Sputum supernatant, IL-8 and mifepristone were assessed for their abilities to prolong the in vitro survival of blood-derived neutrophils.
RESULTThe percentage of sputum neutrophils was significantly higher in severe asthmatics [59.54 (41.99-74.65)%] than mild-moderate asthmatics [30.03 (15.94-47.71)%] and healthy control subjects [29.72(16.53-45.74)%] (P < 0. 01); the level of IL-8 in sputum was significantly higher in severe asthmatics [2907.78 (331.67 - 3457.93) ng/L] than mild-moderate asthmatics [287.58 (130.75-656.84) ng/L] and healthy control subjects [179.2 (58.55-346.59) ng/L] (P < 0.01); the percentages of neutrophilic apoptosis respectively cultured with LPS [(10.57 +/- 1.97)%], severe asthmatics supernatant [(11.82 +/- 2.96)%], IL-8 [(10.47 +/- 1.93)%], dexamethasone [(9.93 +/- 1.95)%], severe asthma supernatant + mifepristone [(12.15 +/- 2.86)%] in vitro were lower than that cultured with PBS [(17.98 +/- 2.27)%], healthy control supernatant [(17.37 +/- 2.50)%], mild-moderate asthmatics supernatant [(16.35 +/- 3.26)%], mifepristone [(17.89 +/- 2.38)%], and dexamethasone + mifepristone [(17.06 +/- 2.59)%] (P < 0.01).
CONCLUSIONSuppression of neutrophilic apoptosis seems to play a potential role in airway neutrophilic inflammation in severe asthmatics, and the level of IL-8 in sputum was significantly higher in patients with severe asthmatics.
Adolescent ; Apoptosis ; Asthma ; metabolism ; pathology ; physiopathology ; Case-Control Studies ; Child ; Female ; Humans ; Inflammation ; Interleukin-8 ; metabolism ; Leukocyte Count ; Male ; Neutrophils ; cytology ; Respiratory System ; metabolism ; pathology ; Sputum ; metabolism
2.Ethacrynic acid inhibits airway smooth muscle contraction in mice.
Xiao-Xue ZHAO ; Wei-Wei CHEN ; Yuan-Yuan CHEN ; Meng-Su LIU ; Meng-Yue LI ; Lei CAO ; Qing-Hua LIU
Acta Physiologica Sinica 2019;71(6):863-873
The aim of this study was to investigate the inhibitory effect and the underlying mechanism of ethacrynic acid (EA) on the contraction in mice. BL-420S force measuring system was used to measure the tension of mouse tracheal rings. The whole cell patch clamp technique was utilized to record the channel currents of airway smooth muscle (ASM) cells. The calcium imaging system was used to determine the intracellular Ca concentration ([Ca]) in ASM cells. The results showed that EA significantly inhibited the high K (80 mmol/L) and acetylcholine (ACh, 100 µmol/L)-induced contraction of mouse tracheal rings in a dose-dependent manner. The maximal relaxation percentages were (97.02 ± 1.56)% and (85.21 ± 0.03)%, and the median effective concentrations were (40.28 ± 2.20) μmol/L and (56.22 ± 7.62) μmol/L, respectively. EA decreased the K and ACh-induced elevation of [Ca] from 0.40 ± 0.04 to 0.16 ± 0.01 and from 0.50 ± 0.01 to 0.39 ± 0.01, respectively. In addition, EA inhibited L-type voltage-dependent calcium channel (LVDCC) and store-operated calcium channel (SOCC) currents in ASM cells, and Ca influx. Moreover, EA decreased the resistance of the respiratory system (Rrs) in vivo in mice. These results indicated that EA inhibits LVDCC and SOCC, which results in termination of Ca influx and decreases of [Ca], leading to relaxation of ASM. Taken together, EA might be a potential bronchodilator.
Animals
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Calcium
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metabolism
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Calcium Channels, L-Type
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Enzyme Inhibitors
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pharmacology
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Ethacrynic Acid
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pharmacology
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Mice
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Muscle Contraction
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drug effects
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Muscle, Smooth
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drug effects
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Respiratory System
;
cytology
;
drug effects
3.The Expression of Adiponectin Receptors and the Effects of Adiponectin and Leptin on Airway Smooth Muscle Cells.
Joo Hwa SHIN ; Jung Ho KIM ; Won Young LEE ; Jung Yeon SHIM
Yonsei Medical Journal 2008;49(5):804-810
PURPOSE: Obesity is a major risk factor for asthma and it influences airway smooth muscle function and responsiveness. Adiponectin is inversely associated with obesity and its action is mediated through at least 2 cell membrane receptors (AdipoR1 and AdipoR2). Leptin is positively associated with obesity. We investigated whether human airway smooth muscle (ASM) cells express adiponectin receptors and whether adiponectin and leptin regulate human ASM cell proliferation and vascular endothelial growth factor (VEGF) release. MATERIALS AND METHODS: Human ASM cells were growth-arrested in serum-deprived medium for 48 hours and then stimulated with PDGF, adiponectin and leptin. After 48 hours of stimulation, proliferation was determined using a cell proliferation ELISA kit. Human AdipoR1 and -R2 mRNA expressions were determined by RT-PCR using human-specific AdipoR1 and -R2 primers. Concentrations of VEGF, monocyte chemotactic protein (MCP)-1 and macrophage inflammatory protein (MIP)-1alpha in cell culture supernatant were determined by ELISA. RESULTS: Both AdipoR1 and AdipoR2 mRNA were expressed in the cultured human ASM cells. However, adiponectin did not suppress PDGF-enhanced ASM cell proliferation, nor did leptin promote ASM cell proliferation. Leptin promoted VEGF release by human ASM cells, while adiponectin did not influence VEGF release. Neither leptin nor adiponectin influenced MCP-1 secretion from human ASM cells. Adiponectin and MIP-1alpha were not secreted by human ASM cells. CONCLUSION: Human ASM cells expressed adiponectin receptors. However, adiponectin did not regulate human ASM cell proliferation or VEGF release, while leptin stimulated VEGF release by human ASM cells.
Adiponectin/metabolism/*pharmacology/physiology
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Cell Proliferation/*drug effects
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Cells, Cultured
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Chemokine CCL2/metabolism
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Chemokine CCL3/metabolism
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Humans
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Leptin/metabolism/*pharmacology/physiology
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Myocytes, Smooth Muscle/cytology/drug effects/*metabolism
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Obesity/metabolism
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Platelet-Derived Growth Factor/metabolism
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Receptors, Adiponectin/*metabolism
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Respiratory System/cytology/metabolism
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Vascular Endothelial Growth Factor A/metabolism
4.TNF-alpha upregulate MUC5AC mucin secretion through COX2/PGE2 mechanism.
Chinese Journal of Applied Physiology 2008;24(1):120-I
Cell Line
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Cyclooxygenase 2
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metabolism
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Dinoprostone
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genetics
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metabolism
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Epithelial Cells
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drug effects
;
metabolism
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Humans
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Mucin 5AC
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genetics
;
secretion
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RNA, Messenger
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genetics
;
secretion
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Respiratory System
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cytology
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Tumor Necrosis Factor-alpha
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pharmacology
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Up-Regulation
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drug effects
5.Effect of antisense oligonucleotides of ryanodine receptor on proliferation and Ca2+i of airway smooth muscle cells.
Ying WANG ; Sui-Yang ZHANG ; Gui-Sheng QIAN ; Xi-Liang WANG
Chinese Journal of Applied Physiology 2005;21(3):324-328
AIMTo investigate the effect of antisense oligonucleotides (ASON) of ryanodine receptor on proliferation and [Ca2+]i concentration of airway smooth muscle cells (ASMCs).
METHODSASMCs were cultivated with collagen enzyme digestion method. Different concentrations of ASON were added to the cultures with Lipofectamine 2000 to observe the ASMCs proliferation using MTS/PES method. The changes of ASMCs [Ca2+]i were also observed by flow cytometry. The expression of mRNA of subtypes of RyR was assayed by RT-PCR.
RESULTSRyR ASON restrained the proliferation of ASMCs, decreased the expression of RyR and reduced the concentration of [Ca2+]i.
CONCLUSIONRyR ASON could inhibit the proliferation of ASMCs by influencing the concentration of [Ca2+]i after excited.
Animals ; Calcium ; metabolism ; Calcium Channels ; Cell Division ; Cell Proliferation ; drug effects ; Cells, Cultured ; Myocytes, Smooth Muscle ; cytology ; drug effects ; metabolism ; Oligonucleotides, Antisense ; pharmacology ; Rats ; Respiratory System ; Ryanodine Receptor Calcium Release Channel ; genetics ; pharmacology
6.Roles of the cross talk between MAP kinases and Keap1-Nrf2-ARE signaling pathways in chronic obstructive pulmonary disease.
Shu-Jun WANG ; Ya-Jun CHEN ; Shan-Shan WANG ; Dian-Lei WANG ; Chen-Yin WANG ; Li-Li YANG ; Jin-Pei CHEN
Acta Pharmaceutica Sinica 2015;50(2):133-140
Chronic obstructive pulmonary disease (COPD), a common preventable and treatable disease, is characterized by airflow limitation that is usually progressive and associated with an enhanced chronic inflammatory response in the airways. Its main pathological manifestations include airway inflammation, mucus hypersecretion, oxidative stress and apoptotic epithelial cells. Recent research suggests that MAP kinases and Keap1-Nrf2-ARE signaling pathway are involved in the pathological process of inflammation and oxidative stress. This review explores the potential role of the cross talk of these signaling pathways in airway inflammation, mucus hypersecretion, oxidative stress and apoptotic epithelial cells. To clarify the roles of cross talk between MAP kinases and Keap1-Nrf2-ARE signaling pathway, we also focus on the drugs related to that in the treatment of COPD, and it provides ideas for more drug research in the treatment of COPD.
Apoptosis
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Epithelial Cells
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cytology
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Humans
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Inflammation
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Intracellular Signaling Peptides and Proteins
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Kelch-Like ECH-Associated Protein 1
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Mitogen-Activated Protein Kinases
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NF-E2-Related Factor 2
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Oxidative Stress
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Pulmonary Disease, Chronic Obstructive
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metabolism
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Respiratory System
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Signal Transduction
7.Effect of all-trans retinoic acid on airway inflammation in asthmatic rats and its mechanism.
Hong FANG ; Hongfang JIN ; Hongwei WANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(3):229-232
The inhibitive effects of all-trans retinoic acid (ARTA) on airway inflammation in asthmatic rats and its mechanism on the basis of the regulation of nuclear factor kappaB (NF-kappaB) were explored. Thirty-two SD rats were randomly divided into 4 groups: control group, asthma group, dexamethasone treatment group and retinotic acid treatment group. The total and differential cell counts in the collected bronchoalveolar lavage fluid (BALF) were measured. The pathological changes in lung tissues were estimated by scoring. The expression of NF-kappaB inhibitor (IkappaBa), NF-kappaB, intercellular adhering molecule-1 (ICAM-1) in lung tissue was detected by immunohistochemical method. The results showed that in the two treatment groups, the total cell counts and proportion of inflammatory cells in BALF were significantly reduced, but there was no significant difference in differential cell counts in BALF between them. The pathological changes in lung tissues in the treatment groups were significantly attenuated as compared with asthma group. Except the epithelial injury in retinotic acid treatment group was milder than in dexamethasone treatment group, the remaining lesions showed no significant difference between them. In the two treatment groups, the expression of IkappaBa was increased, while the expression of NF-kappaB and ICAM-1 decreased with the difference between the two groups being not significant. It was concluded that the similar anti-inflammatory effects and mechanism of ATRA on airway in asthmatic rats to those of dexamethasone were contributed to the increase of cytoplasmic IkappaBa content and suppression of NF-kappaB activation and expression.
Animals
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Asthma
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metabolism
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pathology
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Bronchoalveolar Lavage Fluid
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cytology
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Dexamethasone
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pharmacology
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I-kappa B Proteins
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biosynthesis
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genetics
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Inflammation
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Intercellular Adhesion Molecule-1
;
biosynthesis
;
genetics
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Male
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NF-kappa B
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biosynthesis
;
genetics
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Random Allocation
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Rats
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Rats, Sprague-Dawley
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Respiratory System
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Tretinoin
;
pharmacology
8.Effects of glucocorticoid on IL-13-induced Muc5ac expression in airways of mice.
Jianbo, LIU ; Zhenxiang, ZHANG ; Yongjian, XU ; Lihua, XING ; Huilan, ZHANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(6):575-7
To study the effects of glucocorticoid on the IL-13-induced Muc5ac expression in airways of mice, and investigate its role in mucus secretion of airways, 24 pathogen-free BALB/c mice were randomly divided into 3 groups. IL-13 group received an nasal instillation of 100 microg of recombinant murine IL-13 solution on days 1, 3 and 5. In dexamethasone group, dexamethasone (0.5 mg/kg) was administered intraperitoneally 24 h before and 1 h before the first instillation of IL-13 and on 4 consecutive days (day 0 to day 5, 6 consecutive days in total), while control group was not treated with IL-13 or dexamethasone. Bronchoalveolar lavage fluid (BALF) was collected and eosinophils were counted, and expression of Muc5ac mRNA and protein in lungs were detected by reverse transcription-polymerase chain reaction (RT-PCR) technology and immunohistochemical assay respectively. Our results showed that the number of mice, with positve Muc5ac protein expression, expression of Muc5ac mRNA and eosinophils in BALF after IL-13 treatment were all significantly higher than that of control group (all P<0.01). Despite eosinophils reduced (P<0.01), the number of mice with positive Muc5ac protein expression, expression of Muc5ac mRNA afterdexamethasone treatment didn't decreas significantly as compared with that of IL-13 group. It is concluded that IL-13 can up-regulate the expression of Muc5ac mRNA and protein, which may play a pivotal role in the mucus overproduction of airways. Dexamethasone can suppress IL-13-induced eosinophilic infiltration in lung but can't inhibit the mucus overproduction.
Bronchoalveolar Lavage Fluid/cytology
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Dexamethasone/*pharmacology
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Interleukin-13/*pharmacology
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Mice, Inbred BALB C
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Mucins/*biosynthesis
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Mucins/genetics
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Mucus/secretion
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RNA, Messenger/biosynthesis
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RNA, Messenger/genetics
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Random Allocation
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Respiratory System/*metabolism
9.Effects of glucocorticoid on IL-13-induced Muc5ac expression in airways of mice.
Jianbo LIU ; Zhenxiang ZHANG ; Yongjian XU ; Lihua XING ; Huilan ZHANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(6):575-577
To study the effects of glucocorticoid on the IL-13-induced Muc5ac expression in airways of mice, and investigate its role in mucus secretion of airways, 24 pathogen-free BALB/c mice were randomly divided into 3 groups. IL-13 group received an nasal instillation of 100 microg of recombinant murine IL-13 solution on days 1, 3 and 5. In dexamethasone group, dexamethasone (0.5 mg/kg) was administered intraperitoneally 24 h before and 1 h before the first instillation of IL-13 and on 4 consecutive days (day 0 to day 5, 6 consecutive days in total), while control group was not treated with IL-13 or dexamethasone. Bronchoalveolar lavage fluid (BALF) was collected and eosinophils were counted, and expression of Muc5ac mRNA and protein in lungs were detected by reverse transcription-polymerase chain reaction (RT-PCR) technology and immunohistochemical assay respectively. Our results showed that the number of mice, with positve Muc5ac protein expression, expression of Muc5ac mRNA and eosinophils in BALF after IL-13 treatment were all significantly higher than that of control group (all P<0.01). Despite eosinophils reduced (P<0.01), the number of mice with positive Muc5ac protein expression, expression of Muc5ac mRNA afterdexamethasone treatment didn't decreas significantly as compared with that of IL-13 group. It is concluded that IL-13 can up-regulate the expression of Muc5ac mRNA and protein, which may play a pivotal role in the mucus overproduction of airways. Dexamethasone can suppress IL-13-induced eosinophilic infiltration in lung but can't inhibit the mucus overproduction.
Animals
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Bronchoalveolar Lavage Fluid
;
cytology
;
Dexamethasone
;
pharmacology
;
Interleukin-13
;
pharmacology
;
Male
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Mice
;
Mice, Inbred BALB C
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Mucin 5AC
;
Mucins
;
biosynthesis
;
genetics
;
Mucus
;
secretion
;
RNA, Messenger
;
biosynthesis
;
genetics
;
Random Allocation
;
Respiratory System
;
metabolism
10.Cigarette smoke extract promotes proliferation of airway smooth muscle cells in asthmatic rats via regulating cyclin D1 expression.
Xiao-Yu ZHANG ; Yong-Jian XU ; Xian-Sheng LIU ; Zhen-Xiang ZHANG
Chinese Medical Journal 2010;123(13):1709-1714
BACKGROUNDIncreased proliferation of airway smooth muscle cells (ASMCs) are observed in asthmatic patients and smoking can accelerate proliferation of ASMCs in asthma. To elucidate the molecular mechanisms leading to these changes, we studied in vitro the effect of cigarette smoke extract (CSE) on the proliferation of ASMCs and the expression of cyclin D1, an important regulatory protein implicated in cell cycle.
METHODSASMCs cultured from 8 asthmatic Brown Norway rats were studied. Cells between passage 3 and 6 were used in the study and were divided into control group, pcDNA3.1 group, pcDNA3.1-antisense cyclin D1 (ascyclin D1) group, CSE group, CSE + pcDNA3.1 group and CSE + pcDNA3.1-ascyclin D1 group based on the conditions for intervention. The proliferation of ASMCs was examined with cell cycle analysis, MTT colorimetric assay and proliferating cell nuclear antigen (PCNA) immunocytochemical staining. The expression of cyclin D1 was detected by reverse transcriptase-PCR (RT-PCR) and Western blotting.
RESULTS(1) The percentage of S + G2M phase, absorbance value at 490 nm wavelength (A(490)) and the expression rate of PCNA protein in CSE group were (31.22 +/- 1.17)%, 0.782 +/- 0.221, (90.2 +/- 7.0)% respectively, which were significantly increased compared with those of control group ((18.36 +/- 1.02)%, 0.521 +/- 0.109, and (54.1 +/- 3.5)%, respectively) (P < 0.01). After the transfection with antisense cyclin D1 plasmid for 30 hours, the percentage of S + G2M phase, A(490) and the expression rate of PCNA protein in ASMCs were much lower than in untreated cells (P < 0.01). (2) The ratios of A(490) of cyclin D1 mRNA in CSE group was 0.288 +/- 0.034, which was significantly increased compared with that of control group (0.158 +/- 0.006) (P < 0.01). After the transfection with antisense cyclin D1 plasmid for 30 hours, the ratios of A(490) of cyclin D1 mRNA in ASMCs was much lower than in untreated cells (P < 0.01). (3) The ratios of A(490) of cyclin D1 protein expression in CSE group was 0.375 +/- 0.008, which was significantly increased compared with that of control group (0.268 +/- 0.004) (P < 0.01). After the transfection with antisense cyclin D1 plasmid for 30 hours, the ratios of A(490) of cyclin D1 protein expression in ASMCs was much lower than in untreated cells (P < 0.01).
CONCLUSIONCSE may increase the proliferation of ASMCs in asthmatic rats via regulating cyclin D1 expression.
Animals ; Asthma ; metabolism ; Blotting, Western ; Cell Cycle ; drug effects ; Cell Proliferation ; drug effects ; Cells, Cultured ; Cyclin D1 ; genetics ; metabolism ; Disease Models, Animal ; Female ; Flow Cytometry ; Immunohistochemistry ; Microscopy, Phase-Contrast ; Myocytes, Smooth Muscle ; cytology ; drug effects ; metabolism ; Plant Extracts ; toxicity ; Rats ; Respiratory System ; cytology ; drug effects ; Reverse Transcriptase Polymerase Chain Reaction ; Smoking ; adverse effects ; Tobacco ; chemistry