Background and purpose:Hsp90 is cell chaperone protein that interacts with many proteins,but itself can not degrade its client proteins.Hsp90 inhibitor can inhibit tumor cell proliferation,induce cell apoptosis,cell growth arrest and increase the degradation of Hsp90 client proteins like Survivin.In order to explore the co-effects of Hsp90 inhibitor 17-AAG and Survivin on LoVo cells and the possible mechanisms,we observed the effects of 17-AAG on proliferation and cycles of LoVo cells and the protein level of Survivin.Methods:LoVo cells were treated with 17-AAG.The cell proliferation inhibition rate was evaluated by MTT assay.The cell cycle was detected by ? ow cytometry.The expression of Hsp90 client protein Survivin was detected by Western blot.Results:17-AAG time-dose-dependently inhibit the proliferation of LoVo cells,after 100 ng/ml,500 ng/ml and 800 ng/ml 17-AAG exposure for 24 hrs,the cell proliferation inhibition rate was 21.00%,40.81%,60.34% respectively,after exposure for 48 hrs,the cell proliferation inhibition rate was increased to 27.29%,48.17%,80.97% respectively,after exposure for 72 hrs,the cell proliferation inhibition rate was to 34.45%,67.81%,88.42%;17-AAG arrested cell cycle,when LoVo cells were exposed to 100 ng/ml 17-AAG for 72 hrs,the cell ratio of G0/G1 phase was(61?3)%,when to 500 ng/ml for 72 hrs,cell ratio of G0/G1 phase was increased to(74?3)%,compared to(48.2?0.8)% LoVo cells without 17-AAG(P

AIM: To investigate the effects of a Chinese herb compound prescription, Wen-Jin-Tong(WJT), on the deposition of advanced glycation end products (AGEs) ,the expression of receptor for AGEs(RAGE)and ICAM-1 in cardiovascular tissues of diabetic rats. METHODS: The diabetic rat model was induced by injection of streptozotocin. The quantity of AGEs accumulation, the expressions of RAGE and ICAM-1 in aorta and cardiac tissues were detected using fluorescence method, RT-PCR and in situ hybridization techniques. RESULTS: AGEs accumulation in aorta and cardiac tissues of rats treated with Wen-Jin-Tong was lower than that of diabetic model rats(P

The purpose of this study was to investigate and analyze the high frequency electrocardiogram (HFECG) for middle-aged and elderly people.138 subjects were chosen in our study.The mean age was 60.38 years.Among them,72.1 per cent were between 50 and 69 years.The results showed that (1) the differences were not significant between male and female groups in the mean number of high frequency notches (HFN).(2) For an increase in high frequency notches in the leads V1 and V2,we should consider the possibility of pathological changes of the anterior medial wall of heart,which was supplied by the dessending rami of the right anterior coronary artery,on the one hand,and it might remind us to pay great attention to posteromedian wall of heart,on the other hand.(3) Looking at all age groups in this study,we preliminarily found that the age peak of ischemic heart disease was between 50 and 70 years,according to the number of high frequency notches in the leads of HFECG.(4) The sensitivity of HFECG for diagnosing ischemic heart disease is 40 per cent higher than that of the conventional ECG.(5) In addition,nine leads were used in this examination,which not only preserved sensitivity of six leads but also could provide a localizing value for the diagnosis of heart disease.

ObjectiveTo assess the risk of vector-born infectious diseases by monitoring the major vectors in the relevant venues of the 19th Hangzhou Asian Games in Fuyang competition area, so as to provide references for the prevention and control of vector in similar large-scale events or activities in the future. MethodsFrom April to October 2023, monitoring and evaluation of rodent, mosquito, fly and cockroach density levels were carried out in various venues and reception hotels in Fuyang competition area (venues and hotels were the 1st circle area, and various environments and places in the surrounding 500 meters were the 2nd circle area). SPSS 25.0 software was used to analyze the data, and chi-square test or Fisher’s precision probability test were used to calculate the qualified rate and positive rate. Moreover, the path index, landing index and Bretrau index were analyzed by Kruskal-Wallis H test. ResultsThe control effect of vector density in the 1st circle was significantly better than that in the 2nd circle. Since September, the monitoring indices of the venues in the two circles had reached the national A-level and B-level standard, respectively. The positive rate of rodent traces generally showed a trend of first increasing and then decreasing, and the peak was shown to occur in July and August, with the highest rate of 5.38%. The qualified rate of rodent prevention facilities continued to rise, and facilities in the 1st and 2nd circles had reached national A-level and B-level standard respectively in late August. The landing index and path index of mosquitoes basically showed a changing trend from high to low, with the highest landing index of 4.56 individuals·person-time^-1 and the highest path index of 2.44 places·km^-1.The adult fly infestation rate remained at a low level, with the highest rate of 4.17%, and the minimum qualified rate of fly prevention facilities was 28.57%, which reached the national standard after July. The positive detection rates of adult flies and cockroach traces showed no significant pattern, with the highest rate of 7.00% and 8.33%, respectively. The detection rate of live ootheca was always at a low level. ConclusionThe relevant venues in the Fuyang competition area of the 19th Asian Games in Hangzhou meet national standards in terms of vector control indicators such as rodents, mosquitoes, flies, cockroaches and other vectors. By means of environmental improvement, facilities for preventing rodents and flies, and deepening biological and chemical prevention and control methods, it can be ensured that there will be no vector-borne diseases and infestation incidents during the event.