Background and purpose:Hsp90 is cell chaperone protein that interacts with many proteins,but itself can not degrade its client proteins.Hsp90 inhibitor can inhibit tumor cell proliferation,induce cell apoptosis,cell growth arrest and increase the degradation of Hsp90 client proteins like Survivin.In order to explore the co-effects of Hsp90 inhibitor 17-AAG and Survivin on LoVo cells and the possible mechanisms,we observed the effects of 17-AAG on proliferation and cycles of LoVo cells and the protein level of Survivin.Methods:LoVo cells were treated with 17-AAG.The cell proliferation inhibition rate was evaluated by MTT assay.The cell cycle was detected by ? ow cytometry.The expression of Hsp90 client protein Survivin was detected by Western blot.Results:17-AAG time-dose-dependently inhibit the proliferation of LoVo cells,after 100 ng/ml,500 ng/ml and 800 ng/ml 17-AAG exposure for 24 hrs,the cell proliferation inhibition rate was 21.00%,40.81%,60.34% respectively,after exposure for 48 hrs,the cell proliferation inhibition rate was increased to 27.29%,48.17%,80.97% respectively,after exposure for 72 hrs,the cell proliferation inhibition rate was to 34.45%,67.81%,88.42%;17-AAG arrested cell cycle,when LoVo cells were exposed to 100 ng/ml 17-AAG for 72 hrs,the cell ratio of G0/G1 phase was(61?3)%,when to 500 ng/ml for 72 hrs,cell ratio of G0/G1 phase was increased to(74?3)%,compared to(48.2?0.8)% LoVo cells without 17-AAG(P

AIM: To investigate the effects of a Chinese herb compound prescription, Wen-Jin-Tong(WJT), on the deposition of advanced glycation end products (AGEs) ,the expression of receptor for AGEs(RAGE)and ICAM-1 in cardiovascular tissues of diabetic rats. METHODS: The diabetic rat model was induced by injection of streptozotocin. The quantity of AGEs accumulation, the expressions of RAGE and ICAM-1 in aorta and cardiac tissues were detected using fluorescence method, RT-PCR and in situ hybridization techniques. RESULTS: AGEs accumulation in aorta and cardiac tissues of rats treated with Wen-Jin-Tong was lower than that of diabetic model rats(P

The purpose of this study was to investigate and analyze the high frequency electrocardiogram (HFECG) for middle-aged and elderly people.138 subjects were chosen in our study.The mean age was 60.38 years.Among them,72.1 per cent were between 50 and 69 years.The results showed that (1) the differences were not significant between male and female groups in the mean number of high frequency notches (HFN).(2) For an increase in high frequency notches in the leads V1 and V2,we should consider the possibility of pathological changes of the anterior medial wall of heart,which was supplied by the dessending rami of the right anterior coronary artery,on the one hand,and it might remind us to pay great attention to posteromedian wall of heart,on the other hand.(3) Looking at all age groups in this study,we preliminarily found that the age peak of ischemic heart disease was between 50 and 70 years,according to the number of high frequency notches in the leads of HFECG.(4) The sensitivity of HFECG for diagnosing ischemic heart disease is 40 per cent higher than that of the conventional ECG.(5) In addition,nine leads were used in this examination,which not only preserved sensitivity of six leads but also could provide a localizing value for the diagnosis of heart disease.