OBJECTIVE: To investigate the effect of T lymphoma invasion and metastasis 1 (Tiam 1) overexpression in head and neck squamous cell carcinoma (HNSCC) cells. METHOD: Endogenous expression of Tiam 1 in 8 head and neck squamous cell carcinoma cell (HNSCC) lines was investigated by real-time RT-PCR. A lentivirus vector containing Tiaml was transfected into UM-SCC-47 cells, a head and neck squamous cell carcinoma cell line with little endogenous Tiaml expression. Stable clone, obtained by G418 screening, were assayed by RT-PCR and Western blot to validate the gene expression efficiency. The biological behaviors of the transduced cells were determined by cell counting, MTT and in-vitro migration assay. RESULT: Tiam 1 gene was highly expressed in M2 cell line and it's low level expression was found in UM-SCC-47. Cell counting and MTT assay showed that over-expression of Tiaml significantly promoted cell proliferation (P < 0.05). The cell monolayers overexpressed Tiaml that resulted in a significant increasment of cell migration in infected head and neck squamous cell carcinoma cell lines (P < 0.05). CONCLUSION Tiam 1 gene plays an important role in the growth and migration in head and neck squamous cell carcinoma cell lines. It may be a useful marker for metastasis of head and neck squamous cell carcinoma.
Blotting, Western ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Guanine Nucleotide Exchange Factors ; genetics ; metabolism ; Head and Neck Neoplasms ; metabolism ; pathology ; Humans ; Squamous Cell Carcinoma of Head and Neck ; T-Lymphoma Invasion and Metastasis-inducing Protein 1 ; Transfection

Objective To explore the effects of down-regulated PVT1 on the proliferation,migration and invasion of nasopharyngeal cancer(NPC)C666-1 cells. Methods Expression of PVT1 in NPC cell lines was de-tected by real-time RT-PCR. The effects of PVT1 silencing on cell proliferation were detected using MTT assay. Transwell assay was performed to assess the effect of down-regulated PVT1 on C666-1 cells migration and invasion. Protein level of N-cadherin,Vimentin and E-cadherin were determined by qRT-PCR and Western blot. Results Expression of PVT1 was significantly increased in seven NPC cell lines. Expression of PVT1 was reduced compared to the control group after the siPVT1 transfection. C666-1 cell proliferation was inhibited as compared with siNC group after transfection. The number of migration and invasion cells were significantly reduced while down-regulat-ed PVT1 expression. E-cadherin expression was upregulated while N-cadherin and Vimentinafter was downregulat-ed after PVT1 silencing. Conclusions Downregulation of PVT1 in C666-1 cells via siPVT1 transfection can inhib-it cell growth,migration and invasion,as well as reverse epithelial-mesenchymal transition. PVT1 may serve as a biomarker or even a therapeutic target for NPC.