Objective Amblyopia is common disease in children. It can cause visual suppression that damage binocular vision function especially to the development of stereopsis. The objective of this study was to investigate the stereoscopic vision in children with different types and degrees of amblyopia. Methods This is a clinical retrospective comparative analysis. 178 children with amblyopia from Sichuan Provincial Corps Hospitial CAPF were enrolled in this study. Approximation zero disparity, crossed disparity, uncrossed disparity were examined with Yan' s stereogram. The classification of stereogram based on the criteria of Okuda. The degree of amblyopia was graded based on the corrected vision acuity. Results The differences in the eyes and percentage of approximation zero disparity, crossed disparity, uncrossed disparity among refractive amblyopia group, anisopia amblyopia group and strabismic amblyopia group were statistically significant, respectively(χ~2 =13. 979, P =0. 001; χ~2 = 15. 198, P = 0. 001 ; χ~2 =6. 974, P = 0. 031) . Approximation zero disparity, crossed disparity in mild amblyopia were better than of medium amblyopia in ametropic amblyopic children (P ＜ 0. 05) . No statistically significant difference in the eye number and percentage of uncrossed disparity was found between mild and moderate refractive ametropia (P ＞ 0. 05) . The same outcomes also were seen in approximation zero disparity, crossed disparity, uncrossed disparity between mild and medium anisopia amblyopia (P ＞ 0. 05) . No statistically significant differences in the eye number and percentage of approximation zero disparity, crossed disparity were found between mild and moderate strabismic amblyopia (P ＞0. 05) . Conclusion Amblyopia affects the development of stereoacuity. Amblyopia is aggravated with the increase of amblyopia degrees. The influence of amblyopia on stereoscopic vision is most mild in refractive ametropic and most serious in strabismic amblyopia.