Objective To study the relationship between the levels of serum oxide and antioxide in patients with Wilson disease (WD) and its visceral injury.Methods We determined the serum levels of MDA,GSH PX,GSH and VitE in 29 patients with WD and 21 normal controls by the method of reversephase high performance liquid chromatography (RP HPLC) and the chemo colorimetric method.Results The serum levels of MDA in all patients with WD were significantly higher than those of controls( P 0 05 ).Conclusion There was metabolism disorder of free radicals (FRs) in the serum of patients with WD.As compared with normal controls the serum levels of oxides increased and the levels of antioxides decreased in patients with WD,it might be one of important factors for the patients with visce ral injury.

Objective To summarize MR manifestations of cerebral arteriovenous malformations (AVMs), and to evaluate the accuracy of MR angiography in determining cerebral AVMs’ aspects. Methods Thirty-six cases of cerebral AVMs were analyzed comparatively between MRA and DSA in sizes, feeding arteries and draining veins. Results Cerebral AVMs were appeared in every part of brain. MRI showed honeycombed and linear flow-void low intensity signal in lesion area, showed flow-void signal of expanded thickening vessels, and showed cerebral atrophy or fraction surrounding the lesions. MRA showed that the sizes of most AVMs were 2～5 cm, that the feeding arteries originate from the expansion of original feeding arteries of these areas, that the draining veins drain from the lesions into the surrounding veins or sinuses. The numbers of these vessels were related positively to the sizes of lesions. The differences in showing AVMs between MRA and DSA were that the sizes of lesions on MRA were more than those on DSA, and the numbers of draining veins on MRA were more than those on DSA. Conclusion MRA may show all signs of AVMs, and is not less than, or superior to DSA in their diagnosis. MRI may play important roles in the examination and the diagnosis of cerebral AVMs.

Objective To investigate the role of MRI in diagnosis of primary intracranial teratoma.Methods Nine cases of primary intracranial teratoma proved by operation and pathology were examined by MRI before operation.MRI features were analysed retrospectively.Results The localized and qualitative rates were 100% and 88.8% respectively.The characteristic MRI appearances of primary intracranial teratoma were as follows:(1)The tumor was lobular in shape with clear margin;(2)The tumor had heterogeneous signal intensity ;(3)Inhomogeneous Gd-DTPA enhancement in most of the tumors.Conclusion Multiplanar imaging and Gd-DTPA enhancement in MRI are helpful in diagnosis and differential diagnosis of teratoma.

Objective To observe the therapeutic effect of rude Rhubarb combined with Danshen root treating Cholestatic hepatitis E. Methods 60 cases of cholestatic hepatitis E were randomly recurited into a treatment group and a control group, with 30 cases in each group. The control group was administrated with liver protection therapy, symptomatic treatment and supportive care. Based on therapies of the control group, the treatment group was taken with Rhubarb 3 g orally, twice daily, and intravenously dropped with Danshen root injection 20ml, once daily. Results The total effective rate of the treatment group and the control group was 93.3% and 66.7% respectively, showing significant differance(P<0.01 ). The value of TBil and DBil in the treatment group was statistically improved compared with the control group (P<0.01) . besides the hospitalized time of the treatment group was 46±9 days and hospitalization expense was 6630±1032 RMB, demonstrating a significant difference compared with the control group. Conclusion Rude Rhubarb combined with Danshen root can treat Cholestatic hepatitis E by improving the symptoms and reducing serum bilirubin level. Besides this method also shortens therapeutic time and reduces hospitalization costs.

Objective To explore the role of Kupffer cell-expressed Fas ligand(FasL) on acute pancreatitis(AP),and the protective effect of gadolinium chloride(GdCl3),a Kupffer cell inhibitor,on liver injury during AP.Methods Fifty-four ICR mice were randomly assigned into three groups: healthy control group(n=6);AP group(four time points for observation,6 mice each);Gd+AP group(GdCl3 pretreatment group,four time points for observation,6 mice each).AP was induced in mice using cerulein.Liver parenchymal enzymes(AST,ALT and LDH) and amylase(AMY) were assayed using an automatic analyzer.Serum FasL was assayed with ELISA and FasL protein expression in liver was assayed by Western blotting.Results After induction of AP in mice,the serum levels of AST,ALT,LDH,AMY and liver FasL expression were significantly elevated.Serum FasL levels at 4,8,16 and 24h after AP induction were 505.94?36.21,496.60?33.65,476.64?22.66 and 450.75?38.21,respectively,which were significantly higher than those in healthy control group(83.60?7.75,P

Objective To evaluate the effect of Caspase-1 inhibitor on hepatic tissue and on the expression of hepatic interleukin-18 of rats with experimental severe acute pancreatitis(SAP).Methods Forty-two SD rats were randomly assigned into 3 groups: healthy control group(HC,n=6),SAP+normal saline group(SAP-S,n=18) and SAP+ICE inhibitor group(SAP-ICE-I,n=18).SAP was induced by retrograde infusion of 4% sodium taurocholate into the pancreaticobliliary duct in SD rats.Animals in HC group received similar surgical procedure and duct cannulation but without sodium taurocholate inoculation.In SAP-S group,rats received the first intraperitoneal injection of isotonic saline 2 hours after induction of acute pancreatitis,and the same injection was repeated at 12th hour.ICE inhibitor was injected into the rats in SAP-ICE-I group 2 hours after induction of acute pancreatitis,and the same injection was repeated at 12th hour.Blood samples were obtained from the rats in SAP-S and SAP-ICE-I groups via cardiac puncture at 6,12 and 18h after modeling,respectively.The levels of serum amylase(AMY),alanine aminotransferase(ALT) and aspartate aminotransferase(AST),and the peritoneal fluid,were detected at these three time points.The histopathology of pancrease and liver were observed under light microscope.Intrahepatic expression and localization of IL-18 protein were detected respectively by Western blotting and immunohistochemistry.Results Serum AMY,ALT,AST and peritoneal fluid were significantly increased in SAP-S and SAP-ICE-I groups compared with those in HC group(P

Acute pancreatitis,a multiple system disease was associated with distant organs injury such as the liver,lung and kidney,etc.Acute renal failure is one of the major death causes in severe acute pancreatitis.Renal injury in acute pancreatitis and its pathogenesis was reviewed to further understand the mechanisms of systemic inflammatory response and multiple organ failure

OBJECTIVE: To study the impact and its mechanism of pharmaceutical regulation on the firm performance. METHODS: To make the multiple regression analysis with the pharmaceutical regulation intensity as independent variable, profit rate as dependent variable, R&D innovation and firm investment as intervening variable, based on the pharmaceutical industry data of China. RESULTS: The relationship between the pharmaceutical regulation intensity and profit rate is significantly positive correlation, for which the R&D innocation plays a role of mediation. CONCLUSION: Pharmaceutical regulation stimulates the R&D revenue and enhances technical innovation, and thus the innovation achievement enhances the firm performance. The increase of firm performance brought by innovation compensates the drop by regulation cost.

Objective To study the effect of Caspase-1 inhibitor on severe acute pancreatitis (SAP) in experimental SD rat model. Methods A model of SAP was reproduced by retrograde injection of 5% sodium taurocholate into the biliary-pancreatic duct. Heathy control (HC) rats underwent identical surgical procedure and duct cannulation without the injection of sodium taurocholate. Forty-two SD rats were randomly divided into three groups: healthy controls (HC, n=6); SAP-S group (n=18); SAP-ICE-I group (n=18). In SAP-S group, rats received intraperitoneal injection of isotonic saline 2 hours after induction of acute pancreatitis, and saline injection was repeated after 12 hours. In SAP-ICE-I group, rats were given ICE inhibitor intraperitoneally 2 hours after induction of pancreatitis. As in SAP-S group, this was repeated 12 hours laten. Surviving rats were killed at certain time, and all samples were obtained for subsequent analysis. Also, twenty-four rats were randomly divided into two groups: SAP-S group and SAP-ICE-I group, and the 24-hour death rate after SAP induction was observed. Results The serum amylase levels were increased significantly in SAP-S group (P0.05). Caspase-1 inhibition abated the severity of pancreatic tissue damage, and the 24-hour death rate was lowered from 91.7% to 41.7%. Conclusions The expressions of Caspase-1 activated cytokines IL-1? and IL-18 play a pivotal role in the pathogenesis of SAP. Caspase-1 inhibition significantly abates the severity and the mortality in SAP.

Objective To study the role of Caspase-1 activated cytokines in renal injury of experimental severe acute pancreatitis(SAP).Methods Thirty-two SD rats were randomly divided into four groups:healthy control(HC),SAP 6h,SAP 12h and SAP 18h groups.SAP was induced by retrograde infusion of 5% sodium taurocholate into the bili-pancreatic duct in SD rats.Serum BUN and Cr were determined by an automated HITACHI-7150 analyzer.Serum IL-1? level was determined by ELISA.Myeloperoxidase(MPO)in the kidney was determined by enzyme chemistry assay.Intrarenal expressions of Caspase-1,IL-1? and IL-18 mRNA were assessed by semi-quantitative RT-PCR.Results The serum BUN and Cr levels were increased significantly(P