Objective To investigate the relationship between Toxoplasma gondii(TOX)infection and abnormal pregnancy outcomes. Methods A total of 126 cases of abnormal pregnancy women in the Department of Obstetrics and Gynecology,the Fourth People’s Hospital of Langfang from March to December 2013 were chosen as an experimental group,and 263 cases of normal pregnancy women of childbearing age as a control group. The TOX-IgM and-IgG antibodies were detected by ELISA. The data in the two groups were processed and analyzed by SPSS13.0. Results The positive rates of TOX-IgM,-IgG in 126 cases of abnormal pregnancy women were 7.94%and 19.84%respectively,and 1.90%and 8.75%in the control group respectively,and there were significant differences between them(χ2IgM=6.82,χ2IgG=9.70,both P<0.01). The positive rates of TOX-IgM,-IgG in the normal pregnancy women were lower than those in 4 sub-groups of abnormal pregnancy women,and all the differences were statistically significant(χ2spontaneous abortion = 10.40,χ2premature delivery = 9.03,χ2embryo damage =4.32,χ2birth defect = 4.04,all P<0.05). However,the TOX-IgM,-IgG positive rates in the 4 sub-groups of abnormal pregnancy women had no statistically signifi-cant difference(P > 0.05). Conclusions TOX infection could cause serious abnormal pregnancy outcomes. Therefore,the comprehensive control measures should be strengthened.

Objective To understand the pregnancy outcomes and risk factors in pregnant women infected with Toxoplasma gondii. Methods Toxoplasma IgM and IgG antibodies in sera from 2 740 cases of pregnant women were detected by using enzyme-linked immunosorbent assay(ELISA)in Zhuozhou Municipal Maternal and Child Health Care Center from 2010 to 2013,and the pregnancy outcomes were followed up. The risk factors for Toxoplasma infection were investigated with questionnaires. Results Among the 2 740 cases of pregnant women,195 cases were found with antibodies to T. gondii(7.12%),and among them,44 cas-es were IgM positive(22.56%),and 151 cases were IgG positive(77.44%). There were 41 cases with adverse pregnancy out-comes among the 195 cases(21.02%),including 32 cases of IgM positive(78.05%)and 9 cases of IgG positive(21.95%). There were 6 cases of adverse pregnancy outcomes in uninfected pregnant women(2.86%). The difference between the two groups was statistically significant(P<0.05). The close contact with animals,eating raw meat,eating chafing dish or barbeque,and eating raw meat stuffing were important risk factors in pregnant women infected with T. gondii(compared with the uninfected group,P<0.01). Conclusion The Toxoplasma infection may lead to adverse pregnancy outcomes,therefore,to develop good habits of life and health is an effective way to avoid adverse pregnancy outcomes.

Objective To investigate the distribution of weak D and Del phenotype in unrelated blood donors with negative Rh (D). Methods Four hundred and nineRhD (-) unrelated donors were screened by the saline agglutination method. Type weak D was detected by the indirect antiglobulin test, and type Del was detected by absorb radiation method. Results In 409 RhD (-) samples, 27 cases (6.61%) of type weak D were checked out, and 61 cases (14.91%) were type Del and 321 cases (78.48%) were corroborate for RhD (-), In the confirmed RhD (-) blood donors, phenotype ccee was most (49.14%), followed by Ccee (23.47%), People with phenotype ccEe accounted for 4.16%in type weak D, followed by Ccee (1.71%). Ccee accounted for 10.02%in type Del, and Ccee accounted for 1.71%. Conclusion RhD (-) donors screened by regular testing should adopt a more sensitive test for verifing type weak D or Del. In order to ensure the security of blood transfusion, people with type weak D and Del should be regarded as RhD positive blood donors, and the RhD negative people deemed to be recipients.

Objective To explore the effects and mechanisms of hepatitis B virus-X protein (HBx) on invasion and migration of hepatocellular carcinoma (HCC) cells.Methods The retrospective cohort study was conducted.The clinicopathological data of 30 patients with liver tumor (20 with HCC and 10 with benign tumor of liver) who were admitted to the Affiliated Hospital of Xuzhou Medical College between July 2014 and July 2015 were collected.HCC tissues of 20 patients with HCC (with history of HBV infection) were collected by surgical resection and peritumoral normal tissues (outside of tumor capsule) of 10 patients with benign tumor of liver (without history of HBV infection) were collected.The expressions of epidermal growth factor receptor 3 (ErbB3)in HCC tissues and peritumoral normal tissues were detected by immunohistochemistry (IHC).The relative expressions of ErbB3 and HBx in HCC tissues and peritumoral normal tissues were detected by Western blot,and relative expressions of ErbB3 in HepG2 of which green fluorescent protein (GFP) and GFP-HBx were respectively transfected were detected.The relative expressions of ErbB3 mRNA in HepG2 transfected by GFP and GFP-HBx were detected by real-time polymerase chain reaction (RT-PCR).The migration and invasion of HepG2 were respectively detected by Transwell assay with and without matrix.The measurement data with normal distribution were represented as $± s.The comparisons between groups were evaluated with the independent-sample t test.Correlation analysis was done by the Pearson test.Results (1) The expressions of ErbB3 were detected by IHC:relative value of mean optical density (MOD) of ErbB3 in HCC tissues of 20 patients with HCC and peritumoral normal tissues of 10 patients with benign tumor of liver were 2.54± 1.33 and O.99±0.29,respectively,with a statistically significant difference (t =6.542,P ＜ 0.05).(2) The relative expressions of ErbB3 and HBx were detected by Western blot:relative expressions of ErbB3 and HBx were respectively 0.79±0.13,1.10±0.28 in HCC tissues of 10 patients with HCC and 1.07±0.17,0 in peritumoral normal tissues of 10 patients with benign tumor of liver,with statistically significant differences (t =3.229,19.486,P＜0.05).The results of Pearson test showed that there was a positive correlation of expression between ErbB3 and HBx in HCC tissues (r=O.637,P＜ 0.05).(3) The relative expressions and transcriptional levels of ErbB3 were detected by Western blot and RT-PCR:relative expressions of ErbB3 in HepG2 of which GFP and GFP-HBx were respectively transfected were O.75±0.11 and 1.10±0.10,respectively,with a statistically significant difference (t=4.291,P＜0.05).The relative expressions of ErbB3 mRNA in HepG2 of which GFP and GFP-HBx were respectively transfected were O.38±0.03 and O.94±0.07,respectively,with a statistically significant difference (t=11.703,P＜O.05).(4) The effects of ErbB3 on migration and invasion of HepG2:numbers of transmenbrane cell in HepG2 of which His and His-ErbB3 were respectively transfected by Transwell assay with matrix were respectively 271± 18 and 463± 31,respectively,with a statistically significant difference (t =8.202,P＜0.05).Numbers of transmenbrane cell in HepG2 of which His and His-ErbB3 were respectively transfected by Transwell assay without matrix were respectively 315±38 and 549±34,respectively,with a statistically significant difference (t =8.310,P＜0.05).Conclusion HBx protein can promote the invasion and migration of hepatocellular carcinoma cells through up-regulating expressions of ErbB3 protein.

BACKGROUND:Islet and islet cel transplantation for the treatment of diabetes has achieved effect, but the research is limited dut to the shortage of islet and immune rejection. OBJECTIVE:To observe the effect of transplantation of islet-like cells that in vitro differentiated from bone marrow mesenchymal stem cells on the treatment of diabetes in rats. METHODS:The rat bone marrow mesenchymal stem cells were induced with basic fibroblast growth factors and hepatocyte growth factors, and then received immunocytochemistry staining to detect the induction. The Sprague Dawley rats received intraperitoneal injection of streptozotocin to establish the diabetes models. After modeling, the rats were randomly divided into control group and experimental group (transplanted with induced islet-like cells). The experimental group was transplanted with the induced islet-like cells through renal capsule, and the control group was transplanted with normal saline in the same dose. The blood glucose and body mass of the diabetes rats were observed after transplantation. RESULTS AND CONCLUSION:The bone marrow mesenchymal stem cells could differentiate into islet-like cells after in vitro induced with basic fibroblast growth factors and hepatocyte growth factors. There was no significant change in blood glucose of the control group after transplantation (P>0.05), and the blood glucose of the rats in the experimental group was significantly decreased compared with the control group (P<0.05). The bone marrow mesenchymal stem cells can differentiate into islet-like cells after in vitro induced with the induction system containing basic fibroblast growth factors and hepatocyte growth factors, and the islet-like cells have a certain ability of insulin secretion. The transplantation of induced islet-like cells after transplanted into the diabetes rats through renal capsule can decrease the blood glucose level of the rats.

Objective Based on DRG evaluation indicators,research an evaluation method that can comprehensive-ly evaluate the medical service capacity and efficiency of clinical departments in hospitals.Methods Using the front page of inpatient medical records from a tertiary hospital in Xuzhou in 2022 as a sample,obtaining DRG related indi-cators through the China Healthcare Security Diagnosis Related Groups 1.1 grouping system.Based on the number of DRG,CMI,TEI,and CEI indicators,use TOPSIS method to comprehensively evaluate the quality of medical ser-vices in clinical departments.Results The widest coverage of medical services is in General Surgery,with a DRG group of 325;The most difficult medical service is in the Emergency Department,with a CMI value of 1.45;The best time efficiency is in Ophthalmology,with a TEI of 0.56,The Dermatology has the best cost efficiency,with a CEI of 0.36;TOPSIS comprehensive evaluation shows that the top five are Pediatrics,General Medicine,Emergen-cy Department,General Surgery,and Gastroenterology,and the bottom five are Dentistry,Burn Unit,Orthope-dics,Neurosurgery,and CCU.Conclusion The TOPSIS method based on DRG integrates two dimensions of medical service capability and medical service efficiency,and horizontally compares clinical departments.The evaluation re-sults are more objective,reasonable,and accurate,which can provide a methodological basis for hospital refined management.

Objective Based on DRG evaluation indicators,research an evaluation method that can comprehensive-ly evaluate the medical service capacity and efficiency of clinical departments in hospitals.Methods Using the front page of inpatient medical records from a tertiary hospital in Xuzhou in 2022 as a sample,obtaining DRG related indi-cators through the China Healthcare Security Diagnosis Related Groups 1.1 grouping system.Based on the number of DRG,CMI,TEI,and CEI indicators,use TOPSIS method to comprehensively evaluate the quality of medical ser-vices in clinical departments.Results The widest coverage of medical services is in General Surgery,with a DRG group of 325;The most difficult medical service is in the Emergency Department,with a CMI value of 1.45;The best time efficiency is in Ophthalmology,with a TEI of 0.56,The Dermatology has the best cost efficiency,with a CEI of 0.36;TOPSIS comprehensive evaluation shows that the top five are Pediatrics,General Medicine,Emergen-cy Department,General Surgery,and Gastroenterology,and the bottom five are Dentistry,Burn Unit,Orthope-dics,Neurosurgery,and CCU.Conclusion The TOPSIS method based on DRG integrates two dimensions of medical service capability and medical service efficiency,and horizontally compares clinical departments.The evaluation re-sults are more objective,reasonable,and accurate,which can provide a methodological basis for hospital refined management.

Objective Based on DRG evaluation indicators,research an evaluation method that can comprehensive-ly evaluate the medical service capacity and efficiency of clinical departments in hospitals.Methods Using the front page of inpatient medical records from a tertiary hospital in Xuzhou in 2022 as a sample,obtaining DRG related indi-cators through the China Healthcare Security Diagnosis Related Groups 1.1 grouping system.Based on the number of DRG,CMI,TEI,and CEI indicators,use TOPSIS method to comprehensively evaluate the quality of medical ser-vices in clinical departments.Results The widest coverage of medical services is in General Surgery,with a DRG group of 325;The most difficult medical service is in the Emergency Department,with a CMI value of 1.45;The best time efficiency is in Ophthalmology,with a TEI of 0.56,The Dermatology has the best cost efficiency,with a CEI of 0.36;TOPSIS comprehensive evaluation shows that the top five are Pediatrics,General Medicine,Emergen-cy Department,General Surgery,and Gastroenterology,and the bottom five are Dentistry,Burn Unit,Orthope-dics,Neurosurgery,and CCU.Conclusion The TOPSIS method based on DRG integrates two dimensions of medical service capability and medical service efficiency,and horizontally compares clinical departments.The evaluation re-sults are more objective,reasonable,and accurate,which can provide a methodological basis for hospital refined management.

Objective Based on DRG evaluation indicators,research an evaluation method that can comprehensive-ly evaluate the medical service capacity and efficiency of clinical departments in hospitals.Methods Using the front page of inpatient medical records from a tertiary hospital in Xuzhou in 2022 as a sample,obtaining DRG related indi-cators through the China Healthcare Security Diagnosis Related Groups 1.1 grouping system.Based on the number of DRG,CMI,TEI,and CEI indicators,use TOPSIS method to comprehensively evaluate the quality of medical ser-vices in clinical departments.Results The widest coverage of medical services is in General Surgery,with a DRG group of 325;The most difficult medical service is in the Emergency Department,with a CMI value of 1.45;The best time efficiency is in Ophthalmology,with a TEI of 0.56,The Dermatology has the best cost efficiency,with a CEI of 0.36;TOPSIS comprehensive evaluation shows that the top five are Pediatrics,General Medicine,Emergen-cy Department,General Surgery,and Gastroenterology,and the bottom five are Dentistry,Burn Unit,Orthope-dics,Neurosurgery,and CCU.Conclusion The TOPSIS method based on DRG integrates two dimensions of medical service capability and medical service efficiency,and horizontally compares clinical departments.The evaluation re-sults are more objective,reasonable,and accurate,which can provide a methodological basis for hospital refined management.

Objective Based on DRG evaluation indicators,research an evaluation method that can comprehensive-ly evaluate the medical service capacity and efficiency of clinical departments in hospitals.Methods Using the front page of inpatient medical records from a tertiary hospital in Xuzhou in 2022 as a sample,obtaining DRG related indi-cators through the China Healthcare Security Diagnosis Related Groups 1.1 grouping system.Based on the number of DRG,CMI,TEI,and CEI indicators,use TOPSIS method to comprehensively evaluate the quality of medical ser-vices in clinical departments.Results The widest coverage of medical services is in General Surgery,with a DRG group of 325;The most difficult medical service is in the Emergency Department,with a CMI value of 1.45;The best time efficiency is in Ophthalmology,with a TEI of 0.56,The Dermatology has the best cost efficiency,with a CEI of 0.36;TOPSIS comprehensive evaluation shows that the top five are Pediatrics,General Medicine,Emergen-cy Department,General Surgery,and Gastroenterology,and the bottom five are Dentistry,Burn Unit,Orthope-dics,Neurosurgery,and CCU.Conclusion The TOPSIS method based on DRG integrates two dimensions of medical service capability and medical service efficiency,and horizontally compares clinical departments.The evaluation re-sults are more objective,reasonable,and accurate,which can provide a methodological basis for hospital refined management.