AIM: To investigate the constituents of the effective fractions from Shenshao Xinxin Prescription(Radix et rhizoma ginseng;Radix paeoniae rubra;Radix puerariae lobatae). METHODS: The compounds were isolated by column chromatography on silica gel,polyamide,sephadex gel and their structures were determined on the basis of their spectroscopic evidence including UV,FAB-MS and NMR data. RESULTS: Eight compounds had been isolated and identified as daidzein,4′-O-methyl-daidzin,genistin,PG-3,puerarin,paeoniflorin,daidzein 8-C-Apiosyl(1→6) glucoside,4′,7-diglucoside. CONCLUSION: The compounds obtained were the alcohol-soluble compounds from Shenshao Xinxin Prescription.

Orthogonal design was used to study the extraction technology of Qingyan Xiehuo Granule. The solvent volumn, extracting time, extracting times and smashing degree (only for Fructus Canarii) were treated as the factors and examined at various levels. The extraction technology for Folium Polygoni tinctorii and Radix Scutellariae was determined. The methonal extract absorption (at 310nm) of the dried water extract of Folium Polygoni tinctorii and the content of Baicalin were used as the criteria.

Objective: To study the extraction process of the total flavones of Pollen Typhae ( Typha angustifolia L.). Methods: The optimum extraction process was selected with the orthogonal design. The content of total flavones was determined by UV spectrophotometry. Results: The significant effects of alcohol concentration, extraction time and extraction times on the extraction of total flavones were discovered. Conclusion: The optimum extraction process was as follows: Pollen Typhae was extracted with 70% alcohol for 3 times, with the solvent volume 8, 6, 6 times amount and extraction time 60, 60, 30 minutes in turn.

Object To study the chemical constituents with the antiviral effect from Yinqiaosan on influenza virus Methods Isolation of the constituents by different kinds of column chromatography and elucidation of their structures on the basis of chemical and spectral methods Results Four flavonoid glycosides were isolated and elucidated as 2′ O D apiofuranosyl (1→2) ? D glucopyranosyl] isoliquiritigenin, 4′ isoliquiritigenin, 4′ O D apiofuranosyl (1→2) ? D glucopyranosyl] isoliquiritigenin, 4 isoliquiritigenin, 4 O D apiofuranosyl (1→2) ? D glucopyranosyl] isoliquiritigenin and 4′ isoliquiritigenin and 4′ O D apiofuranosyl (1→2) ? D glucopyranosyl] liquiritigenin, respectively liquiritigenin, respectively Conclusion 2′ O D apiofuranosyl (1→2) ? D glucopyranosyl] isoliquiritigenin is a new naturally compound isoliquiritigenin is a new naturally compound

Objective: To study the adscription of plasma effective constituents of rat after oral administration of alkaloid fraction of Nelumbo nucifera.Methods: Based on the established HPLC analytical method of plasma effective constituents,analysis and comparison were carried out among HPLC profi les of plasma samples obtained after oral administration of alkaloid fraction of Nelumbo nucifera,blank plasma,alkaloid fraction of Nelumbo nucifera.Results: Sixteen compounds were detected under the method,three of them were architypes of compound contained in alkaloid fraction of Nelumbo nucifera,and the others were metabolites.Through the comparison of the UV spectra,seven metabolites remain essential structure because the max wavelength was same to the architypes’,the other six metabolites’ structure changed greatly.Conclusion: Through the study of the adscription of plasma effective constituents of rat after oral administration of alkaloid fraction of Nelumbo nucifera,the structure of metabolites can be conjectured,which may be helpful to study the ADME and mechanism of action.

Objective:To establish a method for controlling the quality of the stilbene fraction of Polygonum multiflorum T hunb. Methods: The contents of total stilbene and 2,3,5,4,-tetrahydroxy-stilbene-2-O-?-D-glucoside were determined by UV and HPLC respectively. Results: As a reference, 2,3,5,4,-tetrahydroxy-stilbene-2-O-?-D-glucoside had a good linear relationship in the range of 3.04-15.20?g/ml and 0.1032-0.6192?g, the linear regression equation was Y=0.0588X+0.0026(r=0.9998) and Y=3614089.84X+14475.21(r=0.9997), the average recovery was 99.95% with RSD=2.11%(n=6)and 100.08% with RSD=2.83%(n=6)for the determination of total stilbene and 2,3,5,4,-tetrahydroxy-stilbene-2-O-?-D-glucoside respectively. Conclusion: The determination results of 3 batches of sample showed that the method was simple, convenient and accurate, and could be used for the quality control of the stilbene fraction of Polygonum multiflorum Thunb.

Objective To study the chemical constituents in the leaves of Nelumbo nucifera. MethodsCompounds were repeatedly purified by chromatography and structures were elucidated by physicochemical properties and spectroscopic analyses. Results Nine alkaloids were isolated and identified as 2-hydroxy-1-methoxy-6-methyl-6a,7-dehydroaporphine (1),armepavine (2),dehydroroemerine (3),dehydronuciferine (4),2-hydroxy-1-methoxyaporphine (5),liriodenine (6),pronuciferine (7),roemerine (8),and nuciferine (9). Conclusion compound 1 is a new aporphine alkaloid named nelumnucine.

AIM: To study the chemical constituents with antiviral activity from Lianqiao Bingduqing Capsule (Fructus Forsythiae) on influenza virus. METHODS: Constituents were isolated by different kinds of column chromatography and their structures were elucidated with chemical and spectral methods. RESULTS: Ten chemical constituents were isolated and elucidated such as forsythoside D, caffeic acid, (+)-phillyrin, (+)-pinoresinal-?-D-glucoside, ?-Hydroxyacteoside, forsythoside C, rutin, forsythoside A. CONCLUSION: ?-Hydroxyacteoside is obtained from Forsythia suspense (Thunb.) Vahl for the first time.

AIM: To investigate the adsorptive and separative properties of 5 kinds of macroporous adsorbing resins to the total saponins and alkaloids, which treated atrophic gastritis with Sanqi Qilian Decoction (Radix et Rhizoma Notoginseng, Radix Astragali, Rhizoma Coptidis.) METHODS: The adsorption capacities, contents and recoveries of the total saponins and alkaloids were selected as the evaluating quotas. The determination of the contents of the total saponins and alkaloids and the investigation into leakage and elution curve were completed with UV-VIS spectrophotometric method. RESULTS: AB-8 resin had the best adsorptive and separative properties to the total saponins and alkaloids in Sanqi Qilian Decoction. The applicable technology was as follows: the concentration of original solution of the extract of Sanqi Qilian Decoction was 40 mg/mL; the largest adsorption capacities on resin to the total saponins and alkaloids were 5.28 mg/mL and 12.38 mg/mL respectively; the eluant was 50% ethanol with 8 times of resin volume, and the elution velocity was 2 mL/min. AB-8 resin could be used for 4 times repeatedly after being reproducted by 95% ethanol and 1 mol/L NaOH. CONCLUSION: Under the process condition which had been selected above, AB-8 resin can be used successfully to adsorb and separate with over 20% of the content of the total saponins Sanqi Qilian Decoction, and 30% of the content of the total alkaloids in the 50% ethanol eluate, and with over 75% of total saponins recovery and 65% of total alkaloids recovery respectively.

Objective To determine the contents of four fiavonoids(trifolirhizin,nor-kurarinone,xanthonumal and kuraridin)in 25 samples of Sophora flavescens Air.in the market.Methods The determination was carried out on Phe- nomenex Luna C_(18)column(250 mm?4.6 mm,5?m),using Acetonitrile-H_2O as mobile phase,at a flow rate of 1.0 mL/min and detected at the wavelength of 310 nm for trifolirhizin,294 nm for nor-kurarinone and,365 nm for xan- thonumal and kuraridin,and column temperature at 35℃.Results The contents of 4 flavonoids in 25 samples of Sophora flavescens Air.in market were in the ranges of 0.05 %～0.32 % for trifolirhizin,0.05 %～0.51% for nor- kurarinone,0.55?10~(-3)%～4.87?10~(-3)% for xanthonumal and 0.06 %～0.23 % for kuraridin.Conclusion The contents of 4 flavonoids in the samples from the market vary notably,and this should be taken into the quality control of Sophora flavescens Ait.