Drag-loaded nanoparticle is a new delivery cartier of drug and biomacromoleculc,which have many fortes such as target ability,permeability,controlled release ability and function of promoting cell differentiation.Polypeptide combined nanoparticles can deliver drugs to the targeted tissues by conjugating with specific ligands.Growth factor combined nanoparticles may have better curative effect by maintaining the activity of the growth factors longer.Nanoparticles can effectively deliver genes to the cells in the injured tissue for the enhancemem of the specific function of the cells.Three dimension nanometer fibrous structure combined with nanoparticle based drug delivery could significantly promote the growth of the cell.Magnetic nanoparticles are widely used for the targeted delivery of drugs or genes and separation of biomolecules.Drug loaded nanoparticles have been increasinsly used in tissue enginerring which have shown a tremendous potential.In the present paper,we reviewed the application of drug-loaded nanoparticles in the field of tissue engineering.

Objective To investigate the protective effect of Ulinastatin on liver function after precise hepatectomy.Methods Fifty-six patients of hepatocellular carcinoma undergoing hepatectomy were divided into Ulinastatin group (U group,28 cases) and control group (group C,28 cases).Results There were no significant differences in liver function and inflammatory factors between the two groups on the 1 st day before the surgry (P＞ 0.05).Serum ALT levels of (231 ±29;140 ±21;56 ± 13;42 ±6) U/L,were lower than group C (267 ± 29;158 ± 23;69 ± 18;53 ± 8) U/L,all P ＜ 0.05;serum AST levels were (175 ±23;94 ±25;47 ± 16;36 ± 8) U/L,lower than group C (191 ± 17;139 ± 16;64 ± 15;46 ±11) U/L,all P＜0.05;Serum TBIL levels were (30 ±6;39 ±9;31 ±9;21 ±6) μmol/L,lower than group C (34 ± 6;46 ± 7;35 ± 8;26 ± 7) μmol/L,all P ＜ 0.05;Serum hs-CRP levels were (52 ± 22;112 ±23;71 ± 16;42 ± 13) rg/L,lower than group C (69 ±23;129 ±25;72 ± 15;49 ± 15) mg/L,all P ＜ 0.05;serum IL-1 β levels were (7.8 ± 0.9;11.1 ± 1.5;8.9 ± 1.6;5.6 ± 0.9) pg/ml,lower than group C (9.2±1.3;13.0 ±2.1;11.1 ±1.4;7.3 ±0.8) pg/rml,all P ＜0.05;Serum IL-6 levels were (187 ±30;76 ±25;46 ± 15;26 ±8) pg/ml;lower than group C(260 ±36;92 ± 16;53 ± 11;30 ±8) pg/ml,all P ＜ 0.05;Serum TNF-α levels were (17 ± 4;12.0 ± 2.4;8.6 ± 2.4;6.7 ± 2.0) pg/ml,lower than group C (25 ± 4;18.7 ± 3.3;15.0 ± 3.1;9.6 ± 1.7) pg/ml,all P ＜ 0.05.Conclusion Ulinastatin protects liver function in perioperative patients undergoing precise hepatectomy.