3.Study of EML4-ALK fusion gene as a biomarker in non-small cell lung cancer.
Lu WANG ; Yao-hui WANG ; Chun-yang LIU ; Mei HAN ; Shu-peng ZHANG ; Ren-sheng LAI
Chinese Journal of Pathology 2011;40(11):788-790
Biomarkers, Tumor
;
metabolism
;
Carcinoma, Non-Small-Cell Lung
;
drug therapy
;
metabolism
;
pathology
;
Humans
;
Lung Neoplasms
;
drug therapy
;
metabolism
;
pathology
;
Oncogene Proteins, Fusion
;
chemistry
;
metabolism
;
Protein Kinase Inhibitors
;
therapeutic use
;
Pyrazoles
;
therapeutic use
;
Pyridines
;
therapeutic use
;
Pyrimidines
;
therapeutic use
;
Smoking
4.One-stage hybrid procedure for treating thoracic aortic pathologies that involve distal aortic arch
Changwei REN ; Xi GUO ; Sheng YANG ; Lizhong SUN ; Lianjun HUANG ; Yongqiang LAI ; Shangdong XU
Chinese Journal of Thoracic and Cardiovascular Surgery 2016;32(12):728-730
Objective This study aims to evaluate the initial results of a hybrid procedure for treating descending thoracic aortic disease that involves distal aortic arch.It also intends to report our initial experience in performing this procedure.Methods A total of 45 patients(35 males and 10 females) with descending thoracic aortic disease underwent a hybrid procedure,namely,thoracic endovascular aortic repair(TEVAR) combined with supra-arch branch vessel bypass,in our center from April 2009 to August 2014.Right axillary artery to left axillary artery bypass(n =20) or right axillary artery to left common carotid artery and left axillary artery bypass(n =25) were performed.The conditions of all patients were followed up from the 14th month to the 77th month postoperative[mean(38.0 ± 17.1) months].Mortality within 30 days,complications such as endoleak after the hybrid procedure,and stenosis or blockage of the bypass graft during the follow-up period were assessed.Results One case of death and one case of cerebral infarction were reported within 30 days.Two patients underwent open surgery beacuse of endoleak.And a newly formed intimal tear was observed in one patient and the patient underwent a second TEVAR during the follow-up period.Condusion Initial results suggest that the one-stage hybrid procedure is a suitable therapeutic option for thoracic aortic pathologies that involve distal aortic arch.However,this procedure is not recommended for type-B aortic dissection,in which a tear is located in the greater curvature or near the left subclavian artery,because of the high possibility of endoleak occurrence.
5.Regulatory effect of Ningxin Hongqi Capsule on local ovarian autocrine and paracrine factors in rats during peri-menopausal period.
Ling XIE ; Ren-Sheng LAI ; Li WANG
Chinese Journal of Integrated Traditional and Western Medicine 2008;28(3):242-244
OBJECTIVETo explore the regulatory effect and mechanism of Ningxin Hongqi Capsule on local ovarian autocrine and paracrine factors in peri-menopausal rats.
METHODSSD female rats aged 4 months were allocated in a normal control group (A) and those aged 14 months with vagino-cytologic figure of oestrus elongation were allocated in a senile female rat model group (B). Rats in Group B were subdivided into 5 groups randomly as the B1, B2 and B3 subgroups treated respectively with high, moderate and low dose Ningxin Hongqi Capsule, the B4 subgroup treated with estradiol and the B5 subgroup untreated for control. Rats' ovaries were obtained at the end of the experiment for observing the conditions of ovarian growing follicles and corpus luteum by HE staining, determining expressions of ovarian estradiol receptor (ER), progesterone receptor (PR), follicle-stimulating hormone (FSH), luteinizing hormone (LH), inhibin alpha (INHalpha), activin (ACT) alpha-beta, follistatin (FS), and insulin-like growth factor (IGF-1).
RESULTSAs compared with Group B5, the ovary index, number of growing follicle were higher and levels of FSH and LH were lower in Group B2 and B3, expression of ER was higher in Group B1 and B4, IGF-1 and INHalpha was higher in Group B2 and B3, and ACTalpha-beta and FS were lower (all P < 0.05).
CONCLUSIONNirigxin Hongqi Capsule could adjust and balance the local ovarian autocrine and paracrine factors to improve the ovarian function.
Animals ; Autocrine Communication ; drug effects ; physiology ; Capsules ; Drugs, Chinese Herbal ; pharmacology ; Female ; Humans ; Models, Animal ; Ovary ; drug effects ; metabolism ; physiology ; Paracrine Communication ; drug effects ; physiology ; Perimenopause ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Receptors, Estradiol ; biosynthesis ; Receptors, FSH ; biosynthesis ; Receptors, Progesterone ; biosynthesis
6.Mutations in the epidermal growth factor receptor and targeted therapy of non-small-cell lung cancer.
Ren-Sheng LAI ; Ling XIE ; Long-Shu SHEN ; Ya-Min HE ; Chang-Le ZHU
Chinese Journal of Pathology 2005;34(11):745-746
Antineoplastic Agents
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therapeutic use
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Carcinoma, Non-Small-Cell Lung
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drug therapy
;
genetics
;
Drug Delivery Systems
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Erlotinib Hydrochloride
;
Female
;
Genes, erbB-1
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Humans
;
Lung Neoplasms
;
drug therapy
;
genetics
;
Male
;
Mutation
;
Protein Kinase Inhibitors
;
therapeutic use
;
Quinazolines
;
therapeutic use
;
Receptor, Epidermal Growth Factor
;
antagonists & inhibitors
;
genetics
7.Pimecrolimus increases the melanogenesis and migration of melanocytes in vitro.
Ping XU ; Jie CHEN ; Cheng TAN ; Ren Sheng LAI ; Zhong Sheng MIN
The Korean Journal of Physiology and Pharmacology 2017;21(3):287-292
Vitiligo is an intriguing depigmentary disorder and is notoriously difficult to be treated. The ultimate goal of vitiligo treatment is to replenish the lost melanocytes by immigration from hair follicle and to restore the normal function of melanogenesis by residual melanocytes. There are two types of topical calcineurin inhibitors called tacrolimus and pimecrolimus, and are recommended as the first-line treatments in vitiligo. Although pimecrolimus is efficacious for the repigmentation of vitiligo, its intrinsic mechanisms have never been investigated in vitro. This research aimed to study the ability of pimecrolimus on stimulating melanogenesis, melanocyte migration and MITF (microphthalmia associated transcription factor) protein expression. Results showed that pimecrolimus at the dosages of 1, 10, 10² nM were neither mitogenic nor cytotoxic to melanocytes. The addition of pimecrolimus at 10, 10² and 10³ nM significantly increased intracellular tyrosinase activity, which was consistent with the elevated content of melanin content at the same concentrations. The peak effect was seen at 72 h in response to 10² nM pimecrolimus. Results of the wound scratch assay and Transwell assays indicate that pimecrolimus is effective in facilitating melanocyte migration on a collagen IV-coated surface. In addition, MITF protein yield reached the highest by pimecrolimus at 10² nM. In brief, pimecrolimus enhances melanin synthesis as well as promotes migration of melanocytes directly, possibly via their effects on MITF protein expression.
Calcineurin
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Calcineurin Inhibitors
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Collagen
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Emigration and Immigration
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Hair Follicle
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In Vitro Techniques*
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Melanins
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Melanocytes*
;
Microphthalmia-Associated Transcription Factor
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Monophenol Monooxygenase
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Tacrolimus
;
Vitiligo
;
Wounds and Injuries
8.Sal I, Nru I and Mse I restriction fragment length polymorphisms of factor IX gene in Chinese Han people.
Zuo-Mu BI ; Bao-Lai HUA ; Ren-Chi YANG ; Hong-Yan WANG ; Wen-Jie WU ; Lin-Sheng QIAN
Journal of Experimental Hematology 2002;10(3):247-250
The purpose of this study is to investigate the Sal I, Nru I and Mse I restriction fragment length polymorphisms (RFLPs) of factor IX gene in Chinese Han people. The frequencies of FIX-192 and FIX-793 for A and G, and FIX-698 for T and C were analyzed by polymerase chain reaction (PCR) in unrelated normal Chinese Han people. A sample of 214, 210 and 206 unrelated X chromosomes were analyzed for FIX-192 and FIX-793 and FIX-698, respectively. The results showed that the frequencies for FIX-192 were 0.878 for A and 0.122 for G, with a heterozygosity rate of 0.213, and the frequencies for FIX-793 were 0.552 for A and 0.448 for G, with a heterozygosity rate of 0.494, the frequencies for FIX-698 were 0.311 for T and 0.689 for C, with a heterozygosity rate of 0.429. It was concluded that the SalIand NruI and MseI RFLPs of FIX gene may be useful markers for carrier detection and prenatal diagnosis in Chinese families with hemophilia B patients.
China
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DNA
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genetics
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metabolism
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Deoxyribonucleases, Type II Site-Specific
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metabolism
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Factor IX
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genetics
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Female
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Gene Frequency
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Heterozygote
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Humans
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Male
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Polymorphism, Restriction Fragment Length
9.Study on the relationship between polymorphism of ApoE gene and TCM syndrome type of primary hyperlipemia.
Wei-Min JIANG ; Shu-Hua TANG ; Ren-Sheng LAI
Chinese Journal of Integrated Traditional and Western Medicine 2006;26(1):38-41
OBJECTIVETo study the relationship between the polymorphism of ApoE gene and TCM syndrome type of primary hyperlipemia.
METHODSApoE genotype of 102 patients with hyperlipemia was detected by gene PCR sequencing.
RESULTSA total of five genotypes were detectable, they were E2/2, E3/3, E4/4, E2/3 and E3/4. The frequency of E3/4 + E4/4 and epsilon4 allelotype detected in the patients of Gan-Shen Yin deficiency syndrome type were significantly higher than those in patients of Pi-Shen Yang-deficiency type or of phlegm stagnation type (P < 0.05, P < 0.01), and which in patients of Qi-stagnation caused blood stasis type were significantly higher than those in patients of phlegm stagnation type ( P < 0.05).
CONCLUSIONPolymorphism of ApoE gene is related in a certain degree to TCM syndrome type of primary hyperlipemia.
Adult ; Aged ; Aged, 80 and over ; Apolipoproteins E ; genetics ; Diagnosis, Differential ; Female ; Genotype ; Humans ; Hyperlipidemias ; diagnosis ; genetics ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Polymorphism, Genetic ; Yin Deficiency ; genetics