1.Primary adenocarcinoma of seminal vesicle: report of a case.
Chinese Journal of Pathology 2011;40(11):775-776
Adenocarcinoma
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drug therapy
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pathology
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surgery
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CA-125 Antigen
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metabolism
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Chemotherapy, Adjuvant
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Diagnosis, Differential
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Genital Neoplasms, Male
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drug therapy
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metabolism
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pathology
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surgery
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Humans
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Keratin-7
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metabolism
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Lymph Node Excision
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Lymphatic Metastasis
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Male
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Membrane Proteins
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metabolism
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Mucin-1
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metabolism
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Neprilysin
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metabolism
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Seminal Vesicles
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pathology
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Young Adult
2.Application of aptamer-based upconverting phosphor biosensing technology in rapid detection for food safety
Shuyue REN ; Qiaofeng LI ; Zhixian GAO
International Journal of Biomedical Engineering 2016;39(6):362-367
The rapid detection technology in food safety plays a vital role in the field of preventive medicine.The traditional physical and chemical detection methods have some limitations,such as costly,difficult to operate and high requirements on environment,can not meet the needs of quality and safety on-site rapid screening,and are gradually replaced by emerging sensor detection technologies.The aptamer-upconversion biosensor technology is a novel rapid detection technology,which is based on the combination of functionalized upconversion nanomaterials and aptamer technology.Compared with the traditional immunofluorescence technology,it has advantages in sensitivity,specificity and stability,and is widely used in rapid detection.In this paper,the upconversion luminescence and aptamer technologies were briefly introduced,and the aptamer-based upconverting phosphor biosensing technology and its application in rapid detection of food safety were summarized.Based on the current research status,the bottleneck and the future development trend of this technology were analyzed,which provides a reference for the application of this technology in food safety and other fields.
3.Quantify the results of mode B ultrasonography, gastric endoscopy and per-splenoportal vein scintig raphy in evaluating their clinical diagnostic value in portal hypertension
Hua LI ; Youan ZHAO ; Li GAO ; Xiaomeng GU ; Changzheng REN
Chinese Journal of Digestive Endoscopy 1996;0(04):-
Y0, then the diagnosis of hypertension with liver cirrhosis obtained. The positive rate of diagnosis is 95% and the specificity is 96% and 91% respectively, much better than those in type B ultrasonography or gastric endoscopy, 78% or 75% respectively (P
4.The investigation on clinical practice pressure and mental health of medical students with type D personality
Li GAO ; Guocai YIN ; Shengli REN ; Jiabao LI ; Hongfeng YANG
Chinese Journal of Behavioral Medicine and Brain Science 2015;24(6):553-555
Objective To investigate the clinical practice pressure and mental health of medical students with type D personality.Methods Type D Scale-14 (DS14) and Beck-Srivastava Stress Inventory (BSSI) test were applied to 371 medical students to assess the personality types and pressure.The symptom checklist 90 (SCL-90) was used to evaluate the psychological health.Results ①The detection rate of type D personality of medical students was 36.39%.②The average score in BSSI of medical students of type D personality was (99.27± 10.51),which was higher than medical students of non-type D personality (87.60± 11.37),and the difference was statistically significant (t=9.9711,P=0.0000).The medical students' score of type D personality in SCL-90 of 9 factors were all higher than medical students of non-type D personality,but the statistically significant difference were only in the score of depression,anxiety and psychosis-like symptoms (t=2.4409,P=0.0151;t=2.8662,P=0.0044;t=2.7783,P=0.0057).Conclusion In face of the same pressure of medical clinical practice,the medical students of type D personality are more likely to have a heavier psychological burden,and the college should pay special attention to the problem and try to intervene the problem,so as to reduce the pressure caused by a variety of psychological problems.
5.Detection, Isolation and Identification of Endophytes in Lolium perenne L.
Li-Ying NIE ; Lei CHEN ; An-Zhi REN ; Yu-Bao GAO ;
Microbiology 1992;0(01):-
A total of 61 Strains were isolated from five perennial ryegrass(Lolium perenne L.)varieties——SR4000, CalypsoⅡ, Pinnacle, Topgun and Justus. By subculture, the stable strains were separated into four morphological groups(MGs). Based on the morphological characteristics and the results of AP-PCR, 57 strains of them were identified as Neotyphodium lolii.
6.Effect of irrigation of peritoneal cavity with warm normal saline on expression of transforming growth factor beta-1 in the peritoneum during the laparoscopic operation
Fei CHEN ; Quangen GAO ; Zeqiang REN ; Xiangnong LI
International Journal of Surgery 2012;39(1):23-26
ObjectiveTo explore the effects of the temperature of saline for irrigation of peritoneal cavity on expression of transforming growth factor beta- 1 in the peritoneum.MethodsSixty patients scheduled for the laparoscopic repair of gastroduodenal benign ulcer perforation were randomized into two groups then surgically treated with various temperature saline.Peritoneal biopsies were taken at the beginning and end of surgery.Tissue concentrations of total and active were measured using enzymelinked immunosorbent assa technique.ResultsAt the start of surgery,there were no significant differences between groups in the total and active fractions of TGFβ1 (P >0.05).At the end of procedure,the peritoneal total( 135.8 ±52.8) pg/mL and active( 136.5 ± 33.0) pg/mL concentrations were significantly lower(P < 0.05) in patients receiving warm normal saline.A light,nonsignificant increase in total(361.3 ± 178.9) pg/mL and active ( 198.3 ± 87.5) pg/mL TGFβ1 levels was observed in patients receiving cold saline(P > 0.05).ConclusionsThe choice of warm normal saline used in irrigation of peritoneal cavity during the laparoscopic operation can decrease peritoneal transforming growth factor beta-1 concentrations.Because of the broad biological effects of TGF31,including regulation of peritoneal healing and oncological processes,this observation may have important clinical perspective.
7.Effects of doxazosin and its enantiomers on the vasoconstriction of rat isolated mesenteric arterioles via α1-adrenoceptors
Lingna GAO ; Tonghui LI ; Jing ZHAO ; Dandan LU ; Leiming REN
Chinese Pharmacological Bulletin 2014;(10):1430-1436
Aim To analyze the blocking effect of ( ± ) doxazosin [ ( ± ) DOX ] , ( -) doxazosin [ ( -) DOX] and ( +) doxazosin [( +) DOX] on the vaso-constriction of rat isolated mesenteric arterioles media-ted by α1-adrenoceptors. Methods The vasoconstric-tion induced by phenylephrine ( Phe) in the rat isola-ted mesenteric arterioles ( the second- and third-order branches) was recorded using DMT wire myograph sys-tem 620M, and theα1-adrenoceptor antagonistic activ-ity of ( ± ) DOX and its enantiomers was analyzed. Results The inner diameter of second- and third-or-der branches of the rat mesenteric artery was (162. 5 ± 5. 3) μm (n=11) and (103. 1 ± 2. 3) μm (n=23), respectively. The values of normalized preload of the second-and third-order branches, which were calculat-ed by the LabChart software, were (2. 93 ± 0. 51) mN ( n =11 ) and ( 2. 64 ± 0. 50 ) mN ( n =23 ) ( P >0. 05 ) . Vasoconstrictive responses to Phe in the sec-ond-order branche of rat mesenteric artery under nor-malized preloads were not significantly different from those under 5 mN preload;however, the Emax values of the Phe-induced vasoconstriction under 10 mN, 15 mN and 20 mN preloads were decreased by 12%, 29%and 43% ( P<0. 01 ) respectively compared with those under normalized preload. The concentration-response curves for Phe were shifted to right in a concentration dependent manner by ( -) DOX or ( +) DOX at 0. 001 , 0. 01 and 0. 1 μmol · L-1 without significant change in their Emax values in the second-and third-or-der branches of rat mesenteric artery. Schild plot anal-ysis indicated that ( -) DOX, ( +) DOX and ( ± ) DOX non-competitively inhibited the vasoconstrictive responses to Phe in the second-order branches, and the rank order of pKB values was ( +) DOX ( 8. 67 ± 0. 10 ) , ( ± ) DOX ( 8. 53 ± 0. 090 ) , ( -) DOX (7. 85 ± 0. 09). However, schild plot analysis indica-ted that ( -) DOX and ( +) DOX competitively inhibi-ted the vasoconstrictive responses for Phe in the third-order branch, and the rank order of their pKB values was ( ± ) DOX ( 8. 68 ± 0. 17 ) , ( +) DOX ( 8. 48 ± 0. 10 ) , ( -) DOX ( 7. 48 ± 0. 140 ) . Conclusion The α1-adrenoceptor blocking activity of ( -) DOX is much weaker than that of ( +) DOX or ( ± ) DOX in the rat isolated mesenteric arterioles, and there is a tendency to enhance the activity of ( ± ) DOX in third-order branches of the rat mesenteric artery though theα1-adrenoceptor blockade effect of ( ± ) DOX is not significantly different from ( +) DOX.
8.Survey on public health education for undergraduate clinical students in 11 medical colleges and universities
Xiaohui REN ; Lin ZHOU ; Bo GAO ; Danping LIU ; Ningxiu LI
Chinese Journal of Medical Education Research 2012;(12):1299-1302
Objectives To learn about current public health education for undergraduate clinical students and to provide some references for developing suitable teaching way in the further.Methods Public health education for undergraduate clinical students in 11 medical colleges and universities and teachers' opinions on it were surveyed with the self-made questionnaire.Quantitative data were analyzed with descriptive statistic method.Results All the surveyed colleges and universities opened public health curriculum for undergraduate clinical students and 10 colleges and universities made public health course to be compulsory.The teaching contents were varied in different colleges and universities.Teachers who gave the public health courses proposed some suggestions on its reform.Conclusions Public health education for clinical students in different colleges and universities has both unity and diversity.It should develop new teaching model based on the training goal of public health education for undergraduate clinical students.
9.Discussion on astronomical background of yiyi calenders chronological era by heavenly stems and earthly branches
Jiucheng JIN ; Guojian GAO ; Hao JIN ; Ren LI
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(05):-
The traditional Chinese medicine deduces the 'Tianshi'based on the chronological era by heavenly stems and earthly branches,whose mechanism has been an eternal mystery.The references discussed the astronomical background of the chronological record of years by heavenly stems and earthly branches.In this paper,① A new law of the nature and human body is unitied was discovered.The relative position of the diurnal motion of the sun and the motion of five yao observed from the earth repeated with a precise period of 60 years,(1years,=360days).It lead the discussion of astronomical background for the chronological record of days and shichen by heavenly stems and earthly branches.② The yiyi calendar's chronological record of months by heavenly stems and earthly branches had the same astronomical background as the chronological record of years,i.e.the relative positions of yearly motion of the sun,the synodic month and the motion of five yao observed from the earth had an approximate cycle time of 60 years.③ The paper further discovered that the relative positions of the diurnal and yearly motion of the sun,the synodic month and the motion of five yao observed from the earth repeated every 240 years.This indicated the astronomical background for the fact that the yiyi calendar's sizhu heavenly stems and earthly branches group had a cycle time of 240 years.④ The traditional Chinese medicine should use the yiyi calendar's local time.
10.The effect of siRNA targeted against Bcl-xl on the expression of apoptosis relevant factors Bcl-xl,Bax, Caspase-3
Wei GAO ; Xin FENG ; Zhenjia YANG ; Yunxia LI ; Libing REN
Chinese Journal of Rheumatology 2013;(1):37-40,封3
Objective To investigate the effect of siRNA targeted against Bcl-xl on cell proliferation of rheumatoid arthritis (RA) and the effect on expres-ion of apoptosis relevant factors Bcl-xl,Bax,Caspase-3.Methods Human RA synovial cells were cultured and passed by tissue block collagenase digestion method.The siRNA plasmid targeting Bcl-xl gene was constructed by Bcl-xl cDNA sequence provided by gene banks,while single missense sequences were used as negative controls.LipofectamineTM 2000 was used to transfect synovial cells.The effect of Bcl-xl silencing on proliferation of synovial cells was evaluated by MTT at 24,48,72 hours after transfection.The expressions of Bcl-xl,Bax,Caspase-3 protein,synovial apoptosisrelated factors were determined by Western blotting after transfected at different time points.The average of multiple-sample average was analyzed by single-factor x2 test or LSD-t and Tamhane's T2 test was used for twotwo comparison.Results MTT result s showed that RNA interference that specifically silent Bcl-xl could obviously suppress the proliferation of sliding film cells,which was most eveident at 48 hours.This inhibition was gradually weakened with prolonged time,but when compared was the control group,differences was significant (P<0.05).Western blotting results displayed that when compared to the control group,Bcl-xl protein expression obviously declined after transfection P<0.01,which was the least at 48 h time point.Bax,Caspase3 protein expression were obviously increased when compared to the coutrol group.Conclusion The expression of Bcl-xl,a RA synovial cell anti-apoptotic factor,is significantly reduced by specific RNA interference silencing Bcl-xl,which may play an important role in inhibiting the excessive proliferation of synovial cells.