Objective:To investigate the expression and clinical significance of microRNA-30a in renal cell carcinoma (RCC). Methods:The miRNA-30a expression in renal tissues and cell lines was detected using quantitative real-time polymerase chain reac-tion (qRT-PCR), and the relationship between miRNA-30a expression and the clinicopathological features of RCC was analyzed. The effect of miRNA-30a on cancer cells was evaluated using methyl thiazolyl tetrazolium (MTT) assay. In addition, a bioinformatics algo-rithm was adopted to predict the potential targets of miRNA-30a. Results:miRNA-30a was downregulated both in RCC tissues and cell lines. Patients with higher miRNA-30a expression exhibited better prognosis than those with lower miRNA-30a expression. Bioin-formatics algorithm and qRT-PCR both indicated that metadherin (MTDH) was a target of miRNA-30a. Moreover, MTT results showed that miRNA-30a could inhibit cell proliferation. Conclusion:miRNA-30a was inhibited in renal cancer, and low miRNA-30a expres-sion was associated with poor prognosis. In addition, miRNA-30a could inhibit the growth of cells through MTDH.

Aim To study the effects of adenosine triphosphate(ATP) on the proliferation of Chinese esophageal carcinoma Eca-109 and hepatoma SMMC-7721 cells.Methods MTT assay was used to determine the inhibition of the proliferation of the two cultured tumor cell 1ines in vitro by ATP,adenosine(ADO) and uridine triphosphate(UTP).Morphological changes of the two cell lines induced by ATP were observed under light microscope.Results ATP(0.03～0.3 mmol?L~(-1)) and ADO(0.1～0.3 mmol?L~(1)) had inhibitory effects on Eca-109 and SMMC-7721 cells concentration-dependently,and the inhibition by ATP was stronger than that by ADO in both cell lines.For Eca-109 cell line,the maximal inhibition rate of the proliferation by ATP and ADO was 86.36% and 29.88%,and the IC_(50) was 0.056 and 0.823 mmol?L~(-1),respectively.For SMMC-7721 cell line,the maximal inhibition rate of the proliferation by ATP and ADO was 82.06% and 52.84%,and the IC_(50) was 0.218 and 0.517 mmol?L~(-1),respectively.UTP had a very weak inhibitory effect on Eca-109 cell line,with the maximal inhibition rate of 18.27%,and did not significantly affect SMMC-7721 cell line.Exposed to higher concentration(0.3 mmol?L~(-1)) of ATP for 72 h,SMMC-7721 cells displayed morphological changes of apoptosis,but Eca-109 cells did not show the characteristics of apoptosis markedly.Conclusion ATP has a strong inhibitory effect on the proliferation of Eca-109 cell line,which is mainly induced by ATP per se,and a metabolite ADO also has weaker effects.For SMMC-7721 cell line,however,ATP inhibits the cell proliferation mainly via its degradation to ADO.

OBJECTIVE:To observe the clinical efficacy and safety of Kudiezi injection combined with alprostadil in the treat-ment of posterior circulation ischemic vertigo. METHODS:Totally 180 patients with posterior circulation ischemia were randomly divided into group A,group B and group C. All patients were given aspirin,atorvastatin,nutrition nerve,antihypertensive,hypo-glycemic and other conventional treatment. On this basis,group A was given Alprostadil injection 10 μg,adding into 0.9% Sodium chloride injection 100 ml,iv,once a day;Kudiezi injection 30 ml,adding into 0.9% Sodium chloride injection 250 ml,iv,once a day. Group B was given Alprostadil injection(the same usage as group A);group C was given Kudiezi injection(the same usage as group A). The course was 14 d. The clinic data was observed,including clinical efficacy;blood flow velocity in left vertebral ar-tery(LVA),blood flow velocity in right vertebral artery(RVA),blood flow velocity of basilar artery(BA),changes of peak laten-cy(PL)and incubation period latency(IPL)before and after treatment;and the incidence of adverse reactions. RESULTS:The to-tal effective rate in group A was significantly higher than group B and C,with significant difference(P<0.05). After treatment,the blood flow velocity of LVA,RVA and BA in each group were significantly higher than before,group A was higher than group B and C;PL-Ⅰ,PL-Ⅲ,PL-Ⅴ,IPL-Ⅰ-Ⅲ and IPL-Ⅲ-Ⅴ were significantly lower than before,group A was lower than group B and C,with significant differences(P<0.05);however,there were no significant difference between group B and C(P>0.05). There were no obvious adverse reactions during treatment. CONCLUSIONS:Kudiezi injection combined with alprostadil has better effica-cy than only Kudiezi injection or alprostadil in the treatment of posterior circulation ischemic vertigo,with good safety.

P 2 purinoceptors were first subdivided into P 2X and P 2Y subtypes, and later this classification was broadened to include P 2T , P 2Z and P 2D subtypes. In the 1990s, a new kind of receptors was found, which respond to UTP, ATP and ATP?S, but not to 2 MeSATP or ?,? MeATP, this finding led to the definition of the so called “P 2U ” or “nucleotide” receptor. Most recent evidence demonstrated the existence of a pyrimidine receptor responding to UTP but not to ATP. For this case, IUPHAR (International Union of Pharmacology) committee recommended that P 2 purinoceptors be subdivided into P2X and P2Y subtypes, any subtypes of intrinsic ion channel be termed P2Xn, and any subtypes of G protein coupled receptor be termed P2Yn purinoceptors. With the development and application of the molecular biologic technique and the cloning and expression of the receptors, the classification was strongly confirmed.

Objective To study the effect of GTA/AVC dual inhibitors on C6 brain nerve glima cells.Methods MTT method was used to test the brain glioma cells activity of proliferation at different times(12 h,48 h,72 h), and using the flow cytometry to detect the change of cells cycle.Results It has obvious inhibitory effcets on C6 brain nerve glima cells, and it was the most obvious at 48 h.GTA/AVC dual inhibitor(2000 μg/mL) treatment group remarkbly rise the G0/G1 rate of celluar, S-phase cell ratio descend, and the cells apoptosis rate was markedly increased.Compared with the control group and the GTA/AVC dual inhibition (1000 μL/mL)treatment group , were significant difference(P<0.05).Conclusion regulating cells cycle progression.

Pediatric medical traumatic stress is a new research which is based on posttraumatic stress,which is attracting more and more nursing workers' attention.The paper gives a brief introduction of pediatric medical traumatic stress and its related symptom,summarizes the achievement abroad,learns the experience of pediatric medical traumatic stress abroad,provides a reference for nursing workers to have a better understanding of pediatric medical traumatic stress in order to let the sick children receive better treatment.

Parenting stress is regarded as a criterion to evaluate the parent's mentation of children with chronic illness,it not only influence parent's care of children,but also decrease the quality of children's life.Si multaneously,it is a main reason of family imbalance.This paper introduces the definition of parenting stress and evaluation tool summarizes the cunent situation of research in abwad,provides a foundation in order to promote the research of parenting stress among caregivers of children with chronic illness.

?Ocular trauma related glaucoma is one of secondary glaucoma, which can lead to serious visual loss. According to the complex clinical findings and pathogenesis of ocular trauma related glaucoma, we divide traumatic secondary glaucoma into hyphema related glaucoma, angle recession related, lens injury related, adhesion and proliferation related. The treatment of secondary traumatic glaucoma with ocular trauma were different, specific treatment measures should be given according to the specific case to protect visual function.

Objective To understand the impacts of a small antimony smeltery on surrounding environment. Methods The samples of raw materials,such as ore and coal,soil and plant around a small antimony smeltery in southwest Guizhou province were analyzed. Results The levels of arsenic,mercury,fluoride ,antimony and selenium in soil around the antimony smeltery significantly increased compared with the average background levels of related elements in nationwide range . The levels of arsenic, mercury,fluoride ,antimony and selenium in soil around the antimony smeltery were 1. 73～4.69,4. 75～237.5,2. 26～3.42,35.6～36. 2,54. 29～57.14 times as much as their related average levels in our country respectively. The levels of arsenic ,mercury ,fluoride and selenium in soil around the smeltery were 1.25～ 3. , 58,1.86～ 93.14,1.38～ 2.08,33. 04～ 34.78 times as much as their related average levels of Guizhou province respectively. The levels of arsenic, mercury, fluoride, antimony and selenium in corn leaves around the smeltery was 67,26.7,1046.4,2220,38. 8 times as much as their related average levels in non-polluted plants respectively. Conclusion The air pollutants,such as sulphur,arsenic,mercury,fluoride,antimony and selenium in industrial waste gas emitted from the antimony smeltery had resulted in heavy pollution to the environment, which was aggravated by the application of coal with high level of sulphur.

This study examined the effects of arsenic trioxide on apoptosis and interleukin4 release in T cells of asthmatic patients in vitro and investigated the role of Bcl2 in the active mechanism. Tcells were isolated from asthmatic patients (n=21) and healthy controls (n=20), and then treated with arsenic trioxide and dexamethasone. Cell apoptosis was measured using fluorescence microscopy, flow cytometry and a cytochrome c ELISA kit. Interleukin4 levels in the serum and in supernatants from T cells were quantified by ELISA. Flow cytometric analysis and immunofluorescence studies were performed to determine Bcl 2 expression. Tcells of the asthmatic patients (I.e. without treatment) exhibited decelerated spontaneous apoptosis after 24 h incubation in vitro when compared to T cells of the healthy controls. With dexamethasone treatment, an increase in apoptosis of Tcells was not significantly different between both groups, irrespective of the method used. Arsenic trioxide treatment, however, significantly increased the apoptosis of T cells of the asthmatic group and showed a slight effect on the control group. In asthmatic patients, elevated levels of interleukin 4 and upregulated Bcl 2 expression were detected. Moreover, in vitro, T cells of asthmatic patients spontaneously released more interleukin4 and exhibited more Bcl 2 expression than T cells from the control group. Arsenic trioxide treatment significantly decreased interleukin4 release and downregulated Bcl 2 expression in asthmatic patients, while it only slightly affected healthy controls. Dexamethasone treatment decreased interleukin4 release in both groups examined. It did not significantly influence Bcl2 expression. These results suggest that arsenic trioxide induces T cell apoptosis and decreases interleukin4 release in T cells of asthmatic patients in vitro and that downregulation of Bcl2 expression may be an important mechanism.