1.Analysis on 154 ADR Cases Caused by Traditional Chinese Medicine in Our Hospital
Guanhua REN ; Suirong YUAN ; Shaocheng WANG
China Pharmacy 2007;0(27):-
OBJECTIVE:To investigate the status quo and causes of adverse drug reaction(ADR) caused by traditional Chinese medicine(TCM) in our hospital.METHODS:154 ADR cases reported in our hospital from Feb.2008 to Jun.2009 were analyzed statistically in respect of age and gender of patients,organs and systems involved in ADR and its clinical manifestation.RESULTS:Main clinical manifestations were lesion of skin and its appendents.Most of ADR cases were caused by TCM injections.CONCLUSION:Great importance should be attached to TCM injections and its monitoring to reduce the incidence of ADR.
2.Expression of hyaluronidase and matrix metaloproteinase-9 in the invasion and metastasis of human breast cancer
Yi WANG ; Guosheng REN ; Yuan SHI
Chinese Journal of Minimally Invasive Surgery 2005;0(09):-
Objective To study the expression of hyaluronidase(HAase) and matrix metalloproteinase-9(MMP-9) in the invasion and metastasis of human breast cancer.Methods The expression of HAase and MMP-9 in human breast cancer was detected using tissue homogenate preparing, enzyme-linked immunoadsorbent assay(ELSA),and immunohistochemistry examination.Results ①The levels of HAase were 4.89?2.55,8.03?2.66,and 12.00?3.96 mU/g in grade Ⅰ,Ⅱ,and Ⅲ of breast cancer,respectively(n=10,20,3),with significant differences between each other(P
3.Effects of Methylene Blue on Intestinal Perfusion and Oxygenation in Dogs With Septic Shock
Tongyue REN ; Yuan LI ; Fang WANG
Journal of Chinese Physician 2001;0(09):-
Objective To study the effects of methylene blue(MB), a soluble guanylate cyclase inhibitor , on intestinal perfusion and oxygenation in dogs with septic shock.Methods A septic shock model was established by lipopolysaccharide infusion.After resuscitation with normal saline infusion,2mg/kg MB was infused within 15min in seven dogs.Blood flow of the superior mesenteric artery (SMA) was measured respectively at baseline,post shock for 1h, after resuscitation and 30min after MB injection.Blood gas analysis was done , and oxygenation was calculated.Results Blood flow of the SMA was significantly decreased by 61 8%(P0 0 5 ) ,butO2 extrincreasedby 14 8% .Conclusions Septicshockindogsresultsinadecreaseinintestinalperfusionandanincreasein oxygenextraction ,whichcanbeimprovedbyMBinjection .Theintestinalperfusionispartlyincreasedbyvolumetherapy .
4.Relationships between ouabain of plasma and tissues and blood pressure in lklc hypertensive rats
Weiqing YUAN ; Hao WANG ; Zhuoren LU ; Yukang YUAN ; Huixu REN
Chinese Journal of Pathophysiology 2000;0(10):-
AIM: To explore the role of endogenous ouabain (EO) in the development of hypertension and its secretion character in 1k1c hypertensive rats(HR). METHODS: EO contents of plasma and tissues in 1k1c HR were detected by ELISA. The relationships between plasma and tissues ouabain and blood pressure were analyzed. RESULTS: EO contents of plasma, heart, kidney, adrenal gland, pituitary and hypothalamus in lklc HR were significantly higher than those of normal rats, especially in the adrenal gland and hypothalamus. EO contents of serum, kidney and hypothalamus were correlated with blood pressure. CONCLUSIONS: EO might play an important role in the development of hypertension in 1k1c hypertensive rats. Adrenal gland might be the major source of EO.
5.The expression of hyaluronidase and VEGF on human breast cancer invasion and metastasis
Yi WANG ; Guosheng REN ; Yuan SHI ; Xiaoyi WANG
Cancer Research and Clinic 2001;0(02):-
Objective To study the effect of hyaluronidase and VEGF on human breast cancer invasion and metastasis. Methods By means of ELSA and immunohistochemistry, hyaluronidase and VEGF were detected in breast cancer. Results Hyaluronidase were (3.99?1.91) mU/g, (8.01?2.59) mU/g and (12.00?3.96) mU/g in gradeⅠ, Ⅱ, Ⅲ of breast cancer respectively. Hyaluronidase levels were significantly elevated in grade Ⅱ,Ⅲ as compared to grade Ⅰ, Hyaluronidase levels were significantly elevated in grade Ⅲ as compared to grade Ⅱ(P
6.Pulse wave velocity, ankle-brachial index and carotid intima-media thickness measurement in the assessment of vascular status in elderly patients with isolated systolic hypertension
Gongxian YUAN ; Hua WANG ; Jianghua REN ; Congxin HUANG ; Yinghui WANG
Chinese Journal of Health Management 2012;06(4):228-231
Objective To evaluate the relation of circadian blood pressure rhythm with vascular status in elderly patients with isolated systolic hypertension.MethodsAnkle-brachial index (ABI),brachial-ankle pulse wave velocity (baPWV) and carotid intima-media thickness (IMT) were measured in 74 elderly patients with non-dipper hypertension (non-dipper group)、78 elderly patients with dipper hypertension ( dipper group),and 72 healthy controls ( control group).Results In comparison with the dipper group,baPWV and IMT of the non-dipper group were significantly increased [ baPWV (1801.61± 314.27) vs (1658.67±274.80) (cm/s)],P<0.01; IMT:(0.96 ±0.22) vs (0.87 +0.19) (mm),(P<0.05),while ABI of the non-dipper group was decreased (1.03 ±0.17 vs1.12 ±0.18,P<0.01).Identification rate was significantly different among 3 groups when baPWV >1400 cm/s (x2 =12.57,P <0.01) or ABI <0.9 ( x2 =9.74,P <0.01).The detection of carotid plaques was also significantly different ( x2 =16.49,P < 0.01).Conclusions These findings suggest that vascular damage could be found in elderly patients with isolated systolic hypertension,especially in those with non-dipper hypertension.ABI,baPWV and IMT measurement may be helpful for the assessment of vascular status.
8.Relationship between the detection of carotid plaque's micro flow signals by B-flow imaging and acute cerebral infarction
Gongxian YUAN ; Yinghui WANG ; Jianghua REN ; Congxin HUANG
Chinese Journal of General Practitioners 2012;11(9):658-660
Objective To detect the micro flow signals of carotid plaque by ultrasound B-flow imaging (BFI) and elucidate its relationship with acute cerebral infarction.Methods A total of 63 patients with carotid plaques were divided into 2 groups.acute cerebral infarction (ACI,n =48) and control (n =15).BFI was used to detect the micro flow signals of plaques and comparisons were made with the categorizing results of contrast enhanced ultrasound (CEU).The data were analyzed between two methods.Results The micro flow signals were detected in 15.7% (22/140)patients in the ACI group vs.2.3%(1/44) in the control group.There were more signals in hypoechoic plaques.The detection percentage of micro flow in grade Ⅲ was significantly higher than that in grades Ⅰ and Ⅱ with CEU (P =0.005,P =0.027).No significant differences existed between grades Ⅲ and Ⅳ (P =1.000).Conclusion BFI can detect effectively the micro flow signals of carotid plaques and this characteristic is correlated with the symptoms of ACI.
9.Clinical analysis of histopathology diagnosis of micro endometrial tissues obtained by endometrial cell collector
Ling REN ; Haiyan WANG ; Yuan WEI ; Jing YANG ; Jian ZHOU
Chinese Journal of Postgraduates of Medicine 2016;39(11):1016-1020
Objective To evaluate the feasibility and application of histopathology diagnosis of endometrial tissues obtained by endometrial cell collector (Jingyou, SAP-1). Methods One hundred and ninety-three patients whose endometrial lesions should be excluded were selected. First, endometrial tissue were obtained from the patients by Jingyou, then they underwent comprehensive curettage under hysteroscopy. The histopathology diagnosis was performed respectively. The specimen satisfaction rate and diagnostic accuracy was analyzed and compared. Results The specimen satisfaction rate of curettage under hysteroscopy was 95.85%(185/193). The specimens of 8 cases were not satisfied because the tissues were not enough. The specimen satisfaction rate of Jingyou was 82.38% (159/193). The specimens of 34 cases were not satisfied, among whom in 10 cases scratches did not throughout the whole palace antrum, and in 24 cases tissues quality were poor. The endometrial thickness in unsatisfactory specimen by Jinyou was significantly thinner than that in satisfactory specimen:(0.64 ± 0.18) cm vs. (0.97 ± 0.43) cm, and there was statistical difference (P<0.05). The diagnostic accordance rate between Jingyou and curettage under hysteroscopy was 79.87%(127/159). The sensitivity of Jingyou from high to low was 94.19% (81/86) in normal menstrual endometrial, 7/10 in endometrial carcinoma/ atypical hyperplasia, 67.86%(38/56) in endometrial hyperplasia and 1/7 in endometrial polyps. Missed diagnosis of jingyou inluded 2 cases of endometrial carcinoma and 4 cases of endometrial atypical hyperplasia. The misdiagnosed rate of high grade endometrial lesions was 6/16, and the patients were misdiagnosed because the tissues were not enough. Four cases of endometrial atypical hyperplasia had underwent conservative treatment of repeated curettage. Conclusions Application of Jingyou can obtain micro endometrial tissues, and the accordance rate of histopathology diagnosis is high with curettage under hysteroscopy. When the collector makes a comprehensive collection to the uterine cavity specimen, it can accurately screen endometrial carcinoma and atypical hyperplasia. The patients who have endometrial atypical hyperplasia and receive conservative treatment and curettage repeatedly curettage, thin endometrium and ultrasonic highly suspected endometrial polyps, are not recommend to use Jingyou to obtain specimen. When the specimen is not satisfied using the collector, additional hysteroscopy should be performed to avoid misdiagnosis of high grade endometrial lesions.
10.EFFECTS OF GENISTEIN ON THE EXPRESSION OF COLLAGEN I AND TRANSFORMING GROWTH FACTOR-?_1 OF OSTEOBLAST
Hong CHANG ; Guowei HUANG ; Li LIU ; Dalin REN ; Yuan WANG
Acta Nutrimenta Sinica 1956;0(02):-
Objective To investigate the effects of genistein(GEN) on the expression of collagen I and transforming growth factor-?1(TGF-?1 ) of osteoblast.Method The secondary generation of skull osteoblast of newborn SD rat was incubated with GEN.The cells were divided into six groups:control group,different dose of GEN(10-8,10-7,10-6,10-5mol/L,respectively) groups and E2 group( 10-10mol/L).MTT(OD),the contents of cell protein,the activity of alkaline phosphatase(ALP),the expression of collagen I and the content of TGF-?1 were detected.Results After 48h and 72h,the MTT(OD) of all GEN group and E2 group were significantly higher than those in control group.The MTT(OD) of control group and 10-8,10-7,10-6mol/L GEN groups in 72h were significantly higher than those in 48 h.The protein of 10-5,10-6 mol/L GEN group and E2 group were significantly higher than those in control group.The ALP activity of all GEN groups and E2 group were significantly higher than those in control group.The level of above indices were correlated with the dose of GEN.The expression of collagen I and the content of TGF-?1 in 10-7,10-6,10-5mol/L GEN group and E2 groups were higher than those in control group.They werecorrelated with the dose of GEN and TGF-?1.Conclusion GEN could stimulate proliferation and differentiation of osteoblast,and enhance the expression of collagen I and content of TGF?-1.Compared with E2,,there were similar effects with the higher dosage of GEN.