Objective To observe the therapeutic effect of the method of soothing liver qi stagnation and invigorating blood circula- tion to remove stasis for depression.Methods Totally 90 depression cases were randomized into treatment group and control group with 45 in each.The treatment group was given modified Xiaoyao Powder(Powder for liver qi stagnation with blood stasis) based on the principle of soothing liver qi stagnation to remove stasis.The control group was given Fluoxetine.The course of treatment was 8 weeks in all.Self-rating Depression Scale(SDS),Hamilton Depression Scale(HAMD),Treatment Emergent Symptom Scale (TESS) and scores of TCM symptoms before and after treatment were recorded for comparison.Results SDS,HAMD,TESS and TCM symptoms were improved after treatment in the treatment group(P

Genomics ; Medicine, Chinese Traditional

Objective To explore the problems in the introducing of complementary food in infants and provide a guidance for cli nical practice.Methods The questionnaire survey was carried out by childhood health doctors in mothers or caregivers on fixed outpatient health care day. The data of survey was entered into computer and analyzed with SPSS 11.5 software.Results 1.Earlier (

OBJECTIVE To investigate the HIV infections states in tumor patients for clinical diagnosis,treatment and to prevent HIV infection in the tumor hospital.METHODS The result of HIV detection in tumor patients from Dec 2000 to Aug 2006 was analyzed by the review statistics analysis.RESULTS Totally 48 101 tumor paients were detected,and the number of tumor patients with positive HIV antibody result was 51(0.106%).Among the positive patients there were 21 cases with blood transfusion history,14 cases with blood donating experience,2 cases with both these two kinds of experiences and 14 cases without the two kinds of experiences.Their rate was separately 41.0%,27.5%,4.0% and 27.5%.Most of the HIV positive patients had no clinical synptoms.CONCLUSIONS The HIV positive rate of patients with blood transfusion or blood donating is significantly higher than the patients without these experiences.The routine detection for the HIV before the operation,blood transfusion or other traumatic detection is very necessary.

Objective To establish a simple and economic method for isolating rat hepatic lipocytes. Method Perfusion of the rat liver with pronase E and collagenase in succession was performed in cycles, and the hepatic lipocytes were isolated and purified by digesting the rat liver with DNase in the presence of the above 3 enzymes, followed by 11% Necodenz density gradient centrifugation. The resulted lipocytes were subjected to routine identification procedures and viability assessment. Results The yield of the lipocytes from each rat was 2×107 to 3×107, with viability over 95% and purity above 90%. Conclusion The method adopted in this study is simple, economic and highly productive.

Objective To establish a simple and economic method for isolating rat hepatic lipocytes. Method Perfusion of the rat liver with pronase E and collagenase in succession was performed in cycles, and the hepatic lipocytes were isolated and purified by digesting the rat liver with DNase in the presence of the above 3 enzymes, followed by 11% Necodenz density gradient centrifugation. The resulted lipocytes were subjected to routine identification procedures and viability assessment. Results The yield of the lipocytes from each rat was 2×107 to 3×107, with viability over 95% and purity above 90%. Conclusion The method adopted in this study is simple, economic and highly productive.

A flavivirus, Culex flavivirus, was first isolated from Chinese mosquitoes with high sequences similarities to those of flaviviruses found in America and Japan. In this study, a total of 48 pools of field-collected mosquitoes were sampled from Dandong of Liaoning Province, China during July to September of 2011. Six isolated viruses showing cytopathic effect (CPE) in C6/C36 cells were tested by reverse transcription polymerase chain reaction(RT-PCR)using Flavivirus genus--specific primers and Culex flavivirus-specific primers and the positive PCR-product was sequenced and compared with the sequences of 10 isolates from GenBank. Phylogenetic tree of NS5 and enevelop genes of flavivirus were constructed. The GenBank accession numbers of NS5 gene were JQ409188, JQ409186, JQ409187, JQ409191, JQ409189 and JQ409190. The GenBank accession numbers of envelope gene were JQ065883, JQ065882, JQ065881, JQ065879,JQ065877 and JQ065878.
Animals ; Base Sequence ; Cell Line ; China ; Culex ; classification ; virology ; Flavivirus ; classification ; genetics ; isolation & purification ; Insect Vectors ; virology ; Molecular Sequence Data ; Phylogeny ; Viral Proteins ; genetics

Objective To observe the levels of alanine aminotransferase(ALT), total bilirubin(TBIL), hyaluronic acid (HA), procollagen type Ⅲ aminoterminal peptide (P Ⅲ NP) and larninin (LN) in the sera of rats infected with Clonorchis sinensis (C. sinensis) after treatment of albendazole combined with milkvetch root. Methods Thirty-two healthy adult Wistar rats were randomly divided into four groups with 8 in each based on body mass: control group, non-treatment group, Albendazole group(ALB group) and albendazole combined with milkvetch root group(ALB+MR group). The rats in non-treatmen, ALB and ALB+MR groups were infected orally with metacercariae of C. sinensis 50 per rat. The rats in control group were mock-infected with saline. The rats in ALB group were treated to each rat with 50 mg/kg alhendazole for 5 days, and ALB+MR groups were given to same treat with albendazole, meanwhile each rat injected with 800 mg/kg milkvetch root intraperitoneally for 30 days. All rats were killed after infestation 14 weeks and their sera samples were collected to detect ALT, TBIL, HA, PⅢNP, LN. Results There were statistically significant differences in the levels of ALT and LN in the sera of rats between groups(F=31.40,11.82, P<0.01). Compared with control[ (47.88±4.88)U/L, (51.20±4.12)μg/L], the levels of ALT and LN in rats in non-treatment group [(85.50±9.65)U/L, (64.20±4.18) μg/L] and ALB group [(65.29± 7.78) U/L, (58.23±2.55) μg/L] were significantly increased (P<0.05). Compared with non-treatment group, the levels of ALT and LN in rats in ALB group and ALB+MR groups[(50.25±9.29)U/L, (53.68±5.63)μg/L] were significantly decreased(P<0.05), and they decreased more obviously in ALB+MR group (P<0.05). There were statistically significant differences in the levels of TBIL, PⅢNP and HA in the sera of rats between groups (χ2=15.309,21.418,19.759, P<0.01). Compared with control[(0.700±0.350)μmol/L, (26.085±4.075)μg/L, (81.935±42.550)μg/L], the levels of TBIL, PⅢNP and HA in rats in non-treatment group(2.400 μmol/L, 46.220 μg/L,310.885 μg/L) and ALT group(1.200 μmol/L,36.540 μg/L, 178.010 μg/L) were significantly increased(P<0.05). Compared with non-treatment group, the level of TBIL in ALT+MR group(0.750 μmol/L), the levels of pⅢNP and HA in ALT and ALT+MR group(30.470,100.240 μg/L) were significantly decreased(P< 0.05). The levels of TBIL, PⅢNP and HA decreased more obviously in ALB+MR group(P<0.05). Conclusions The liver function in rats infected with C. sinensis is abnormal. The liver function and fibrosis are improved after treatment with albendazole or albendazole combined with milkvetch root. The treatment of albendazole combined with milkvetch root is more effective.

Primary liver carcinosarcoma is extremely rare.We report a case of liver carcinosarcoma in a 58-year-old man,which was identified by pathologic and immunohistochemical(IHC)examinations.Plain CT scans showed a hypodense mass in the right liver lobe adjacent to the gallbladder fossa.The triple.phase contrast CT scans showed a mixed density mass with inhomogeneous enhancement.Multiple cystic nodules with irregular rim enhancement of the margin located in the tumor.The gallbladder wall and transverse colon were involved.CT presentations of Iiver carcinosarcoma were unspecific and the preoperative diagnosis is difficult.

Objective To investigate the effects of autologous bone mesenchymal stem cells(MSC) transplantation on malignant arrhythmia induced by electrophysiological (EP) stimulation and cardiomyocyte ion channels remodeling in a mini-swine model of acute myocardial infarction (AMI). Methods Immediately after AMI(LAD occluded for 120 min),MSC(10×107,labeled by colloidal gold and cocultivated with 5-azacytidiRe,5-aga,n=12) or equal volume saline (n=10) were iniected through over-the-wire (OTW) balloon in LAD at distal over D1.EP stimulation is performed after 2 hours and 4 weeks in both groups to induce arrhythmia.The variance of heterogeneity of sodium currents(INa)and INa steady-state inactivation curves in different zones of infracted wall were investigated by patch clamp technology and the relationship between ionic channel and ventrieular arrhythmia is analyzed.Results EP induced malignant ventficular arrhythmia (VT) rate was similar (MSC 75% vs.saline 90%.P=0.455) at 2 hours post AMI and was significantly lower in MSC group(25% vs.80%,P=0.012) at 4 weeks post AMI.The Peak INa current densities of the Endo,Media and Epi were significantly lower in MSC group[(-14.04±3.82)pA/pF,(-29.26±5.70)pA/pF,(-12.43±3.04)pA/pF] compared those in saline group [(-9.71±3.38)pA/pF,(-18.98 ±4.05)pA/pF,(-8.47±3.34)pA/pF,all P<0.05].The IHa steady-state inactivation curves of the Epi,Endo and Media in mini-swine with VT in MSC group [(-126.2±10.9)mV,(-106.7±11.9)mV,(-105.4±11.0)mV] were similar as those in saline group with VT[(-129.1±10.9)mV,(-112.2±9.9)mV,(-109.7±9.2)mV,all P>0.05] while significantlylower compared to MSC group without VT[(-93.1±13.8)mV,(-95.2±15.5)mV,(-103.4±8.7)mV,all P<0.05].The multiple logistic regression analysis showed that INa current density (RR=1.449,95%CI 1.276-2.079,P=0.029) and INa steady-state inaetivation curves(RR=1.092,95%CI 1.008-1.917.P=0.012) were the independent factors for reduced VT.Conclusions Autologous MSC attenuated malignant ventricudar arrhythmia induced by EP at 4 weeks in nlini-swine with AMl which might due to altered eardiomyocyte ion channels remedcling induced by MSC.