Objective To detect the effect of osthole on proliferation and apoptosis of HL-60 cells and its molecular mechanism.Methods HL-60 cells proliferation was measured through the CCK8 assay method.The cell morphological changes were observed by Hoechst33342 staining after 8 h of drug effect.Induction of apoptosis was determined by flow cytometry and fluorescent microscopy.Expressions of Bcl-2 and Bax mRNA were evaluated by RT-PCR,and the expressions of cleaved caspase-3,caspase-8,caspase-9,Fas and FasL were evaluated by using western bolt assay.Results Osthole could inhibit the proliferation of HL-60 cells,the maximum inhibiting rate was (90.7 ±4.5)％,F =138.46,P =0.000; the apoptosis rate was 33.6％,F =27.75,P =0.006.The changes of apoptosis of cells and nucleus were shown in cell morphological observation.Osthole affected the decrease of the mRNA levels of Bcl-2 and the increase of the Bax mRNA levels via a dosedependent manner(F =210.12,P =0.000).Western blotting demonstrated that osthole could lead to the increase of the expression levels of cleaved caspase-3,caspase-8,caspase-9,Fas and FasL in the HL-60 cell line via a time-dependent manner.Conclusion Data suggests that osthole inhibits proliferation and induces apoptosis of HL-60 cells through mitochondria-dependent pathway and death-receptor pathway.

Objective To Study wheather the endogenous arginine vasopressin(AVP)is involved in the effect of oxotremorine(a muscarinic receptor agonist)-induced hypothermic response.Methods Core temperature and motor activity were monitored in undisturbed rats using radiotelemetry.Effect of AVP V1 antagonist on oxotremorine(OXO)-induced changes in body temperature and motor activity were observed in the rats.Results Administration of OXO led to a marked hypothermia.Core temperature recovered to basal levels at 4 hours after OXO administration.AVP V1 antagonist blocked markedly the hypothermia effect of OXO.Conclusion The AVP V1-receptor antagonist block the hypothermic effect of OXO,which suggests that OXO-induced hypothermia is mediated by AVP releasing.

Objective To understand the health status of medical staffs in a tertiary hospital of Chongqing City to provide the basic information and scientific basis for formulating the interventional measures.Methods The physical examination data of 1 397 medical staffs in 2013 and 2 178 medical staffs in 2015 were selected as the research objects and performed the summary analysis according to the abnormal indexes distribution,sex and age.Results A total of 1 397 persons completed the physical examination in 2013,the abnormal indexes were detected in 1 250 people,the detection rate of sub-health status was 89.48％;2 178 people in 2015 completed the physical examination,the abnormal indicators were detected in 2 046 people,the detection rate of sub-health status was 93.94％.The comparison of two-year health examination data showed the different degrees of increasing trend in bilateral breast hyperplasia,gallbladder polyps,thyroid cysts,low density lipoprotein cholesterol.Conclusion The health status of medical staffs is not optimistic,so the self health care consciousness of medical staffs needs to be elevated.

Objective To observe the changes of the intercellular spaces of squamous epithelium of lower esophagus in gastroesophageal reflux disease(GERD).Methods Eleven outpatients with GERD symptoms more than 3 months [6 with nonerosive reflex disease(NERD)and 5 with erosive esophagitis(EE)]and 5 healthy volunteers were recruited.All of them underwent endoscopy and 24-hr ambulatory pH monitoring.Biopsies were taken in lower esophagus(2 cm above Z-line)for electron microscope examination.Results Intercellular spaces of esophageal epithelial cell in volun teers,NERD patients and EE patients were (0.374?0.073)?m,(1.308?0.079)?m and (1.332?0.144)?m respectively,with significant differences between the control group and the NERD or EE group.There was no difference between NERD group and EE group.Conclusions Dilated intercellular spaces were seen in both NERD and EE cases,which was significantly different from the control cases.

BACKGROUND:The mechanical properties of monkeys are the closest to the human body.Monkey is the ideal animal model of osteoporosis research.OBJECTIVE:To explore the biomechanical properties of the long bones and vertebrae in rhesus monkeys.DESIGN.TIME AND SETTING:The mechanics experiment for the study was based on monkeys,which was completed in the South Medical University,Key Laboratory of Medical Biomechanics in Octobor 2006.MATERIALS:Four male macaca mulattas aged 17.5 years on average and three female crab-eating monkeys aged 9 years on average.METHODS:Long bones(femur,tibia,fibula,humerus,radius and ulna)and the third and fourth lumbar vertebrae of three adult crab-eating monkeys and four old macaca mulattes were teken to do four-point bending test,torsion test,indentation test and compression test respectively.MAIN OUTCOME MEASURES:①The results of compression experiments and indentation experiments in monkey vertebrae.②The maximum load and the rigidity factor in left side of long bone of monkeys.③The maximum torque(N·m)in the right side of long bone of monkeys.RESULTS:①In the four-point bending tests,the maximum Ioad of the left ulna,fibula,humerus,femur,tibia and radius in macaca mulattas were(574.16±163.53),(179.98±38.32),(1487.9±965.12),(1928.60±336.23),(1303.23±969.35),(559.92±1.12)N,respectively.While the rigidity factor of the left femur,tibia,fibula,humerus,radius and ulna in macaca mulattas were (53.49±14.22),(28.41±5.86),(114.22±13.24),(142.16±18.56).(101.11±15.46),(69.13±5.54)N/mm,respectively.The maximum load of the left femur,tibia.fibula,humerus,radius and ulna in crab-eating monkeys were(179.93±19.38),(53.82±5.31),(631.61±225.81),(726.07±245.69),(424.52±49.s0),(1 91.97±67.73)N,respectively;however,the rigidity factor of the left femur,tibia,fibula,humerus,radius and ulna in crab-eating monkeys were(21.45±2.63),(16.25±6.66),(68.5±12.22),(76.79±14.01),(41.80±2.79),(64.31±15.89)N/mm,respectively.②In the torsion test,the maximum torque of the right fibula,humerus,femur and tibia in macaca mulattas were(1.55±0.82),(22.26±4.26),(30.93±6.54),(17.49±4.04)N·m,respectively.The maximum torqua of the right fibula,humerus,femur,tibia in crab-eating monkeys were(0.81±0.15),(10.34±2.06),(11.58±0.76),(6.68±1.34)N·m,respectively;③In the compression test,the maximal compression load and the rigidity factor of L4 in macaca mulattas were (2811.21±403.90)N,and(69.47±8.92)N/mm,respectively;the maximal compression load and the rigidity factor of L4 in crab-eating monkeys were(1659.90±339.08)N,and(36.29±6.61)N/mm,respectively.④In the indentation test,the maximal indentation and the maximal anti-pressure of L_3 in macaca mulattas were(521.90±38.94)N,and(699.16±43.46)MPa,respectively;the maximal indentation and the maximal anti-pressure of L_3 in crab-eating monkeys were(614±145.94)N,and (815.92±193.69)MPa,respectively.CONCLUSION:Experimental data derived from the experiments demonstrated that rhesus monkeys as a kind of nonhuman primate animals have an important reference value in the animal studies of osteoporosis.

Objective:To compare the therapeutic effects between sequential therapy of external-internal fixation and internal fixation alone in the treatment of high-energy pilon fracture.Methods:A total of 61 patients with high-energy pilon fracture were enrolled by our team for this retrospective analysis who had been treated from January 2015 to July 2017. They received sequential therapy of external-internal fixation (the sequential group) or internal fixation alone (the internal group). In the sequential group of 26 cases, there were 19 males and 7 females (aged from 18 to 65 years), 4 cases of type C1, 8 cases of type C2 and 14 cases of type C3 by the OTA classification, and 7 cases of closed injury and 19 cases of open injury. In the internal group of 35 cases, there were 25 males and 10 females (aged from 19 to 64 years), 6 cases of type C1, 13 cases of type C2 and 16 cases of type C3 by the OTA classification, and 21 cases of closed injury and 14 cases of open injury. The 2 groups were compared in terms of postoperative infection, fracture reduction, fracture union time, nonunion, American Orthopedic Foot and Ankle Society (AOFAS) ankle-hindfoot score, short form 36 health survey questionnaire (SF-36) and reduced range of motion between healthy and affected ankles.Results:There was no significant difference between the 2 groups in gender, age, fracture type, injury cause or follow-up time ( P>0.05), but a significant difference in soft tissue injury favoring the sequential group ( P=0.010). There were no significant differences between the 2 groups in postoperative infection rate [15.4% (4/26) versus 17.1% (6/35)], fracture reduction, fracture union time [(7.4±3.4) months versus (6.5±3.2) months], nonunion rate [7.7% (2/26) versus 8.6% (3/35)], AOFAS ankle-hindfoot score (71.7±29.4 versus 74.4±19.5), or SF-36 (83.1±9.9 versus 83.8±7.9) ( P>0.05). The reduced range of motion between healthy and affected ankles at 6 months postoperation in the sequential group (34.6°±7.2°) was significantly greater than that in the internal group (23.7°±5.1°) ( P<0.05), but there was no significant difference between the 2 groups in the reduced range of motion between healthy and affected ankles at 2 years postoperation (26.0°±11.1° versus 21.8°±11.3°) ( P>0.05). Conclusion:Although both sequential therapy of external-internal fixation and internal fixation alone can lead to fine clinical efficacy in the treatment of high-energy pilon fracture, the former may be more suitable for the patients with severe soft tissue injury.

ObjectiveTo analyze of the characteristics of cognitive function in major depressive disorder patients with suicidal ideation using repeatable battery for the assessment of neuropsychological status ( RBANS),and to explore the risk factors of suicidal ideation Methods 132 depressive patients with suicidal ideation and 162 depressive patients without suicidal ideation were evaluated by RBANS,Using SPSS13.0 to statistic the results,including t test,chi-square test and multiple logistic analysis.Results 1.There were no significant differences in age,gender,education level,marital status,total disease duration,whether for the first time episodes of depression,whether a positive family history of depression between the two groups (P ＞ 0.05 ).The HAMD and HAMA total score were greater in patients with suicide ideation than that without suicidal ideation(P ＜ 0.01 ).2.The score of attention was lower in patients with suicide ideation (98.56 ± 17.80) than in patients without suicide ideation (105.06 ± 14.34) (P =0.001 ).The score of delay memory was lower in patients with suicide ideation (80.39 ± 15.36) than in patients without suicide ideation (85.96 ± 12.55) (P=0.001 ).3.Logistic regression analysis showed that attention deficit and HAMD total score were the risk factor of suicide ideation in major depressive patients.ConclusionAttention deficit may exist in major depressive patients with suicide ideation,and attention deficit maybe one of the risk factor of suicide ideation.

Objective To investigate the status of nurses' resilience and to explore the related factors.Methods 1356 nurses from 11 level Ⅱ and level Ⅲ hospitals of Guangzhou were assessed by using the general information questionnaire and the Chinese version of Conner-Davidson resilience scale.Results The score of nurses' resilience was(59.99±13.59)points and it was significantly lower than that of the domestic norm.There was statistical difference of the score of nurses' resilience on different genders,educational background,professional titles,hospital level and night shifts.There was a positive correlation among educational background,night shifts and nurses' resilience,and a negative correlation between hospital level and nurses' resilience.The hospital level,night shifts and educational background were the influencing factors of nurses' resilience.Conclusions The level of nurses' resilience is low.The higher hospital level and educational background,and the less night shift,the higher of nurses' resilience.

Objective:To investigate the role of reducing resistance and distraction in rapid teeth movement and its reliability by establishing the Beagle dogs’ experimental model.
Methods:The left or right sides in mandibles of 20 beagles were randomly operated with different treatments:distraction twice a day through reducing resistance;distraction 6 times a day through reducing resistance;conventional distraction through reducing resistance;and conventional distraction (the control group). Each treatment was carried out in 10 sides. The pulp vitality, tooth mobility and distance of teeth transportation were evaluated at different time points:before the distraction, distraction after 15 days, retaining 30 days after 15 days of distraction. The degree of inclination, root resorption and alveolar bone density of the compressive areas were evaluated by cone-beam computed tomography images.
Results:The distance of teeth transportation was similar in groups distraction twice daily and 6 times a day through reducing resistance (P>0.05), but their speed of transportation was significantly higher than that of conventional distraction through reducing resistance. The conventional distraction group had the lowest speed of transportation. The pulp vitality of distracted teeth was normal, and no root comprehensive resorption and periodontal defect were found. Distracted teeth in the reduced resistance and distraction groups (13.9°±3.5°) tipped more that in the conventional distraction group (6.6°±1.3°) (P<0.05).
Conclusion:Reducing resistance and distraction are inseparable factors to realize fast teeth moving. The rate of orthodontic tooth movement can be accelerated through resistance reduction and periodontal distraction without obvious unfavorable effects but at minimal acceptable teeth inclination.

s parameters of CEUS between ITBL group and control groups reflect the characterization of flow perfusion in ITBL, which can provide the objective evidence in the diagnosis of ITBL.