As a pattern of manifestation of cerebral small vessel disease,cerebral microinfarct(CMI) is a very common neuropathological change in old people. It is significantly correlated w ith cognitive decline and dementia. This article review s the meuropathology, neuroimaging and clinical significance of CMI.

Objective To assess the effectiveness of the acupuncture treatmeat on anxiety compared with non-acupuncture intervention. Method All of the randomized or quasi-randomized trials involving were reviewed systemantically with the Meta analysis method. The homogeneity test was mode, the standandised mean difference (95% CI) and add risk (OR, 95% CI) were tested. Results Homogeneity test was made among the trials and no significant difference between the acupucture and non-acupuncture groups. Fixed effect model was used. ORP =1.759 419 579, 95% CI (1.336 391, 2.316 356), there was significant difference between the acupuncture group and medicine group as the interventions used to treat anxiety while there was no difference between the two group on the influence in HAMA. Conclusion The Meta analysis results was a trend in favor of acupuncture effectiveness. It seems no serious adverse reactions have been found. But there was no sufficient reliable evidence due to the low quality of the trials and possible publication bias. Further randomized, double blind controlled trials are needed.

Objective To optimize an integration technique for extracting the liposoluble and water-soluble components of Salvia miltiorrhiza. Methods Salvianolic acid B and Tanshinone ⅡA were selected as marker components and determined by HPLC to optimize the integration extract process of Salvia miltiorrhiza by orthogonal test. Results The liposoluble and water-soluble active components of Salvia miltiorrhiza were efficiently extracted by the optimum integration technique. The integration technique for extracting was obtained:Salvia miltiorrhiza was added with 8 times alcohol of 70% and extracted 1 hour for 2 times. Conclusion The liposoluble and water-soluble active components of Salvia miltiorrhiza can be extracted simultaneously by the novel extraction process which was reasonable and feasible. This new technique can be employed to reduce time, working and energy, and be suitable for the morden production.

Purpose: To explore the relationship between the effect of docetaxel inducing apoptosis of non-small-cell lung cancer cell and intracellular level of reactive oxygen. Methods: SPC-AI lung cancer cells were treated with 10 -8mol/L docetaxel for 24 hours to observe the apoptotic morphological change under the electromicroscope and fluorescence microscope in ritro. The treated cells were harvested to analyze the cell cycles using flow cytometry and to estimate the intracellular reactive oxygen levels by staining with 2', 7-dichlorodihydrofluorescein diacetate and dihydroethidine. Results: After SPC-AI lung cancer cells were exposed to 10 ~ mol/L docetaxel for 24 hours, the typical apoptotic morphological changes were observed under the electromicroscope and fluorescence microscope. There was a higher reactive oxygen level in docetaxe-treated cells group than in control group. After 24 hours docetaxel exposure, there was a significant G2 ~'M phase arrest of SPC-AI lung cancer cells. Conclusions: Docetaxel can induce apoptosis in SPC-AI lung cancer cells through the mechanism of G2 ~ M phase arrest and by elevating intracellular level of reactive oxygen.

OBJECTIVE:To prepare Sophora alopecurodies suppository for treating prostatitis chronica and observe its clinical therapeutic effect METHODS:The content of the total alkaloid in the suppository was determined with acid dye spectrophotometry and the therapeutic effect was observed in comparison with qianliean suppository RESULTS:The linear range of TASa was 0 14～15 4?g/ml,standard oxymatriner's recovery was 101 2%,RSD=0 55% CONCLUSION:This suppository is simple in preparation,reliable in quality-control It is slight in irritation to rectum and effective in clinical use The preparation is worth popularizing in clinical practice

Objective To explore the pathognomonic findings of anaphylactic shock in forensic medicine. Methods Animal model of anaphylactic shock was developed in guinea pigs. Substance P level in plasma was determined radioimmunoassay, and detected in laryngopharyneal tissue and the lungs by immunohistochemical SABC method followed by image analysis using BI - 2000 image analyzing system. Positive index (PI) was calculated. Results There was a statistically significant difference for substance P concentration in plasma between the guinea pigs with anaphylactic shock (131.01? 18.93pg/?l)and the control animals (87.70 ?7. 60pg/?l) (P

Objective To explore the survival mechanism of hippocampal ne urons after damage of hypoxia-ischemia and reperfusion of brain.Methods Seven days old SD rats(n=56) were randomly divided into hypoxia-ischemia br a in iniury(HIBD) group and sham group.The HIBD and reperfusion model was establis hed.The flowing of blood was de tected by multicolor Doppler.The p-CREB(phosphorylated c-AMP response element bi nding protein)and c-Jun were immunohistochemically evaluated in hippocampus.Thi onin staining was used to observe the apoptosis.Results The expression of p-CREB reache d the peak at 3,24 h postreperfusion in the right hippocampus of HIBD group,and then decreased to the normal level on the 7th day.In contral group the same reg ions showed basic immn-noreactivity.While c-Jun reached the peak at 6 h postreperfusion,then with a slightly decrease at 24 h;and at 48 h the other peak appeared,then with a gradual decline .On the 7th day the mumber of positive cells were still significanthy more than control group(P0.05).The sham animal showed very few apoptosis cells in the regio ns of hippocampus.Conclusions The persistent activation of CREB in the hippocampus regulates,the expression of c-Jun through the signal transductions and is involved in the course of neuron s′ survival and repair during the period of post hypoxia-ischemia reperfusion.I t is very important for the protection of the pyramidal hippocampal neurons on t he damaged side,especially for the sensitive region CA1. J Appl Clin Pediatr,2005,20(2):133-135

Abstract:The present case report retrospectively analyzed 36 cases of injury to posterior pelvic dng who received treatment in the Department of Orthopedics,Yan'an Hospital between January 2004 and April 2007. According to the Denis classification,there were 20 cases of type I injury to posterior pelvic ring,12 of type Ⅰ,and 4 of Ⅲtype Ⅲ; according to Tile classification,there were 16 cases of B3,10 of C1,6 of C2,and 4 of C3. Pedicle screw fixation system was employed. Two curvy incisions were made on the bilateral posterior superior lilac spine and a hole was chiseled on each side to fit the pedicle screw. Then,a universal pedicle screw was driven between the inner and outer lilac plates until the screw were sunk. A rod was finally traversed through erector spinae to connect two pedicle screws. Thirty cases were available at follow-up with a mean of 11 months (range:6-11 months). None of 30 cases presented with nerve or vessel injury and breakage,loosening of screws,or redislocation of sacroiliac joints. According to functional rating scale,excellent results were found in 15 cases,good in 13,and fair in 2 cases. These findings suggest that treatment for injury to posterior pelvic ring with pedicle screw fixation system is simple and reliable with microtrauma and little bleeding; in addition,it affords good recovery and can be employed in primary hospital without monitoring of X-ray,so it will deserve popular practice.

Objective To investigate the effect of pre- hospital emergency care programs in children with febrile seizures,in order to reduce damage by febrile seizures and obtain the best firstaid effect. Methods 112 cases of children with febrile seizures were treated in pediatric department of our hospital from April 2009 to February 2011.The parents of 75 children patients were given guidance of prehospital emergency care,they were set as the pre-hospital emergency care group.The other 37 cases were set as the non-pre-hospital emergency care group,routine emergency care was given until the medical personnel arrived at the spot.The conditions of aspiration,tongue bite injury,another episode of febrile seizures and brain damage were recorded in two groups of children. Results Only one case of aspiration and three cases of febrile seizures occurred in the pre-hospital emergency care group by misoperation of parents.Non brain damage and tongue bite injury occurred.The incidence rate of improper consequences was 5.3％in the pre-hospital emergency care group,lower than that of the non-pre-hospital emergency care group,51.4％. Conclusions To give pre-hospital emergency care guidance to parents of children patients by mobile phones before the health care workers reach the spot can significantly reduce accidental injury of children,stabilize the emotion of parents,decrease the impact of seizures on children,all the above has a positive effect on follow-up treatment.

Objective To detect the expression of apoptotic inhibitor protein Livin in different esophageal epithelial lesions and to analyze the relation between the expression of Livin with pathologic characteristics. Methods The expressions of Livin mRNA and Livin protein were detected by real- time fluorescence quantitative PCR and western blot in 40 patients with different esophageal epithelial lesions including normal, atypical hyperplasia, carcino-ma in stiu and invasive carcinoma. Results The expressions of Livin mRNA were progressively increased from nor-mal to invasive carcinoma( 1.00 ± 0. 00,2.26 ± 0.79,7.24 ± 1.06,12.21 ± 2.47 ). There was statistical signifi-cance in Livin mRNA expression among carcinoma in stiu and invasive carcinoma and normal tissues ( P < 0.05 ). Conclusion The expression of Livin is significantly related to the progression of esophageal cancer,which may be a new target for diagnosis and gene treatment of esophageal carcinoma.