1.Application of Chlorine Dioxide Generator in Water Disinfection
Yi CHENG ; Linghua REN ; Zewen ZHAO
Journal of Environment and Health 1989;0(06):-
Objective To know the probability of water disinfection of railway water supply provided for themselves by using the chlorine dioxide generator based on chemistry way. Methods According to Technical Standard for Disinfection, filter membrane method was used. The water samples were collected from the railway water supply stations, the germicidal tests were conducted in laboratory and on site. Results In a contact time of 0.5 min, the disinfectant solution containing chlorine dioxide of 0.25 mg/L produced by the generator could meet the requirement of disinfection of drinking water. The generator could maintain chlorine dioxide at 0.3-0.5 mg/L in the pipeline of the water supply. The water quality was up to standard after disinfected. Conclusion The chlorine dioxide generator is suitable for water disinfection of railway water supply provided for themselves.
2.The effect of intensive atorvastatin treatment on kidney function in patients after percutaneous coronary intervention
Yuebin LIU ; Ziping CHENG ; Ren ZHAO
Acta Universitatis Medicinalis Anhui 2014;(3):350-353
Objective To investigate whether intensive atorvastatin treatment in patients after percutaneous coro-nary intervention ( PCI) could decrease the effect of contrast medium on kidney function and the incidence of con-trast-induced acute kidney injury( CI-AKI) . Methods A total of 128 patients with PCI were randomly divided into two groups:the enhanced treatment group (n=64) and the control group(n=64). The enhanced treatment group received 80 mg atorvastatin at 12~24 h before PCI and 24,48 h after PCI. The control group was given 20 mg ator-vastatin respectively before and after PCI. The primary end point was the incidence of CI-AKI. Serum creatinine (Scr), cystatin C, glomerular filtration rate(eGFR), urinary albumin and urinary β-2 microglobulin levels were observed at 24 h before PCI and 24, 48, 72 h after PCI. Results In the enhanced treatment group 3. 1 % (n=2) of patients developed CI-AKI versus 4. 7 % (n=3) in the control group, without statistical difference (P=1.00). There was no significant difference between two groups in postoperative Scr, cystatin C, eGFR, urinary al-bumin, urinary β-2 microglobulin and creatine kinase(CK). Three days after the operation, alanine aminotrans-ferase ( ALT) elevated in two groups, and aspartate aminotransferase ( AST) increased in the enhanced treatment group (P<0. 05), but they were all in the normal range. Conclusion There has been no significant difference in decreasing the incidence of CI-AKI and the damage of contrast medium on renal function between the enhanced treatment group and the control group before PCI.
3.Delicacy management practice of NSFC application process in an affiliated hospital of a medical university
Niannian LI ; Heng WANG ; Jiayun ZHAO ; Ren YE ; Mengming CHENG
Chinese Journal of Medical Science Research Management 2017;30(4):278-281
Objective summarize the delicacy management practices through the NSFC application procedures in a university affiliated hospital,to provide further reference for improvement of the NSFC project approvai ratio.Methods To summarize the management experiences by analyzing the approval number,project category and funds of NCFS from 2006 to 2015.Results The delicacy man agement practices include cultivate scientific research atmosphere by strengthen motivation,initiate application as early as possible,enlarging application number by extensive mobilization,improve applica tion quality by massive training,multi-round updates,tutorial,prevent avoidable errors by cross-over review.Conclusions Delicacy management by scientific research management department during the application process is critical to improve the project approval ratio of NCFS in a hospital.
4.A case report of iliac osteoid osteoma.
Ji CHENG ; Liu-Long ZHU ; Pan ZHAO ; Wen-Cheng REN ; Shao-Bo ZHOU
Journal of Zhejiang University. Medical sciences 2009;38(1):113-114
5.SWOT Analysis of Patent Strategy Implementation in the Manufacturing Industry of Chinese Patent Medicines in Guangdong Province
Huafeng PAN ; Yan YAN ; Yichong FENG ; Bing CHENG ; Jinling REN ; Ziming ZHAO ; Yaosheng TU
World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(4):783-786
This study was aimed to analyze the environment and situation of patent strategy implementation of manufacturing industry of Chinese patent medicines in G uangdong province . This article pointed out that G uang-dong Chinese patent medicines manufacturing implementation patent strategy faced huge opportunities and chal-lenges. It was suggested that the fully understanding, mastering and using of beneficial conditions as well as con-trolling and eliminating the adverse factors will contribute to the better implementation of Chinese patent medicines manufacturing patent strategy in G uangdong province .
6.Effects of umbilical cord-derived mesenchymal stem cells on matrix metalloproteinases secreted by fibroblast-like synoviocytes from rheumatoid arthritis
Lu ZHANG ; Wei KONG ; Yile REN ; Xuebing FENG ; Cheng ZHAO ; Lingyun SUN
Chinese Journal of Rheumatology 2014;(10):665-669
Objective To explore the effects of umbilical cord-derived mesenchymal stem cells (UCMSCs) on fibroblast-like synoviocytes(FLSs) from patients with rheumatoid arthritis(RA). Methods collagen-induced arthritis(CIA) models were developed on Wistar rats and 1 ×106 UCMSCs were given by intravenous injection from tail vein on the 17th day. On day 42, rats were sacrificed and synovial tissues were obtained to detect matrix metalloproteinase (MMP)-1, MMP-3 and MMP-13. Synovial tissues from patients with RA and osteoarthritis(OA) treated by knee arthroplasty were used to isolate FLSs. RNA of FLSs were extracted to compare MMP-1, MMP-3 and MMP-13 expression. FLSs and UCMSCs were cocultured through transwell for 72 hours. Then levels of MMPs were compared in the supernatants by Luminex. The MMPs expressed by FLSs and soluble factors expressed by MSCs were detected by real time-polymerase chain reaction(PCR). After adding antibodies to soluble factors, the MMPs expressions of RA FLSs were compared. Results MMP-1 (6.9±5.4, 1.3±1.4, P<0.05), MMP-3 (6.0±6.5, 1.4±1.0, P<0.05) and MMP-13 (21.8± 20.8, 1.5±1.6, P<0.05) expression were much higher in CIA rats compared with healthy controls. MMP-1 (1.3±1.4, 6.9±5.4,8.7±6.8, P<0.05), MMP-3(1.4±1.5, 6.0±6.5, 6.0±5.7, P<0.05) and MMP-13(3.0±3.2,
22±21, 22±26, P<0.05) expression were inhibited by UCMSCs in vivo. In vitro, MMP-1 (1.8±0.9, 0.9±0.7, t=2.44, P<0.05), MMP-3(2.6±1.7, 1.1±1.0, t=2.25, P<0.05) and MMP-13(2.4±2.3, 0.6±0.7, t=2.37, P<0.05) levels were higher in RA than OA FLSs. After coculture, MMP-13(1.3±1.2, 0.9±1.2, t=3.63, P<0.05) expressed by FLSs were down-regulated, however MMP-1 (1.5±1.4, 6.6±6.0, t=3.90, P<0.05) and MMP-3 (7±17, 22±35, t=2.86, P<0.05) were up-regulated when analyzed by paired t-test. Soluble factors such as I-DO, HGF and IL-10 were elevated. Anti-IL-10 antibody could decrease the function of UCMSCs by inhibiting MMP-13 expression in RA FLSs. Conclusion UCMSCs ameliorates RA by secreting soluble factor IL-10, which may inhibit MMPs expressed by FLSs.
7.Expression of interleukin 27 in the sera of patients with Sj(o)gren's syndrome and its regulation on interleukin 10
Lu ZHANG ; Yile REN ; Cheng ZHAO ; Xia LI ; Xuebing FENG ; Lingyun SUN
Chinese Journal of Rheumatology 2013;17(11):743-746,封3
Objective To measure the level of interleukin (IL)-27 in sera of patients with Sj(o)gren's syndrome (SS) and investigate its role in IL-10 in CD3+CD8-T cells.Methods Serum levels of 1L-27 and IL-10 from 34 SS patients and 33 healthy controls were tested by enzyme linked immunosorbent assay (ELISA).Peripheral blood monocytes (PBMCs) from SS patients were collected and cultured in different conditions (one with PHA,the other with PHA and IL-27).Seventy-two hours later,cells were harvested and the percentages of CD3 +CD8-IL-10+ cells were measured by flow cytometry.Expressions of c-Maf,IL-10 transcription factor,were examined using real-time polymerase chain reaction (PCR).The data were analyzed by t test,Pearson's correlation analysis and multiple linear regression analysis.Results Serum level of IL-27 in patients with SS [(158±34) pg/ml] was significantly higher than that in healthy controls [(139±18) pg/ml,t=2.823,P<0.01].IL-27 level was positively correlated with IL-10 and IgG levels (r=0.384,P<0.05; r=0.354,P<0.05),but negatively correlated with SSDAI (r=0.503,P<0.01).Multiple regression analysis showed independent correlation of IL-27 with IL-10 and SSDAI in SS patients (β=0.412,P<0.01; β=-0.525,P<0.01),but not IgG.CD3+CD8-IL-10+ cells was upregulated by IL-27 in vitro (P<0.01,n=14),so was c-Maf (P<0.01,n=22).Conclusion IL-27 could ameliorate disease activity of Sjogren's syndrome.
8.A survey of perception of common people in Chongqing area about some major diseases
Fan ZENG ; Wanting XIE ; Xiaobin CHENG ; Jun XIAO ; Qian REN ; Ping ZHAO ; Huadong ZHOU ; Yanjiang WANG
Chongqing Medicine 2014;(12):1485-1487,1490
Objective To investigate the fear and attitude of the common people to some threatening diseases .Methods Accord-ing to the age and sex structure of the whole nation ,500 common people were randomly enrolled in the public places of Chongqing main urban area .Firstly ,the respondents were asked to name three diseases they feared most .Then their fear degrees to eight im-portant diseases were performed the questionnaire survey .Results The top ten diseases that common people fear most by their own words were cancer ,AIDS ,heart disease ,hypertension ,stroke ,leukemia ,chronic liver diseases ,diabetes mellitus ,dementia and de-pression successively .Meanwhile ,their fear to the eight important diseases ,which was determined by the ratio of people who did fear to all ,were cancer(94 .2% ) ,traffic accidents(89 .2% ) ,heart disease(85 .0% ) ,AIDS(79 .4% ) ,tuberculosis(76 .8% ) ,hepatitis B(76 .6% ) ,Alzheimer′s disease(73 .4% ) and depression(69 .0% ) .Further analysis showed that such fear was significantly influ-enced by age ,sex and education level .Conclusion The knowledge and perception of common people about certain diseases is one-si-ded ,and their health awareness should be improved .
9.Expression and clinical significance of phosphorylation of mammalian target protein of rapamycin in human glioma
Gang CHENG ; Junlong XU ; Xueguang ZHANG ; Xin LI ; Yubo REN ; Lianqun ZHANG ; Cunyou ZHAO ; Xueyuan LI
Chinese Journal of Postgraduates of Medicine 2012;35(2):3-7
ObjectiveTo investigate the association of phosphorylation of mammalian target protein of rapamycin (pmTOR) expression with glioma malignancy grades,and the correlation of pmTOR expression with Survivin and Ki-67,which represent tumor cell anti-apoptosis ability and reproductive activity.MethodsImmunohistochemistry EliVision method was employed to detect the expression of pmTOR,Survivin and Ki-67 in paraffin tissues from 87 patients with glioma (grade Ⅰ - Ⅱ 27 cases,grade Ⅲ24 cases and grade Ⅳ 36 cases).The association between positive expression rate,level of pmTOR and malignancy grades,and the correlation of its expression level with Survivin and Ki-67 were further evaluated.Results There was no significant difference in the positive expression rate of pmTOR among grade Ⅰ - Ⅱ(77.8%,21/27),grade Ⅲ(75.0%,18/24) and grade Ⅳ (72.2%,23/36) (P > 0.05).However,the significant association between pmTOR expression level and malignancy grades was observed.The expression from 87 patients with glioma was significantly positively correlated with Survivin and Ki-67 expression level (r =0.858,P < 0.01 ;r =0.708,P < 0.01 ).ConclusionsThe expression level of pmTOR is associated with malignancy grades,tumor cell anti-apoptosis ability and reproductive activity.pmTOR may be served as a useful marker for predicting the biological behavior of glioma and a useful target for gene therapy.
10.Correlation of Fatigue with Health-promoting Lifestyle in Undergraduate Students
Liuguo WU ; Mingman CAO ; Xiaomin SUN ; Shengwei WU ; Jingru CHENG ; Tian WANG ; Xiaoshan ZHAO ; Ren LUO
Journal of Guangzhou University of Traditional Chinese Medicine 2015;(3):426-429
Objective To explore the correlation of fatigue with health-promoting lifestyle in the udnergraduate students, so as to provide scientific evidence for the intervention. Methods A cross-section trial was carried out in the undergraduate students from a medical university in Guangzhou. By a self-designed questionnaire, the incidence of fatigue in the undergraduate students was figured out after data processing and statistical analysis. Results A total of 6 100 questionnaires were released, and 5 843 were responded, with a respondent rate being 95.79%. The number of effective questionnaires arrived 5 676. The results showed that the prevalence of fatigue among undergraduate students was 49.6%. The female had a higher risk than the male ( 51.6% vs 45.8%, P<0.01). The prevalence of fatigue in grade 1-3 students was 49.4%, 48.1% and 52.1% , respectively, the difference being insignificant among the three grades (P>0.05). The students with poor level of health-promoting lifestyle had a higher risk of suffering from fatigue (OR=6.647, 95%CI: 4.913-8.993). Conclusion Fatigue is prevalent among the undergraduate students. The incidence of fatigue can be reduced by increasing the level of self-actualization, physical exercise and pressure management.