Objective To establish a HPLC method for the determina-tion of aconitine, hypaconitine, mesaconitine, benzoylaconitine, benzoylhypacoitine and benzoylmesaconine in traditional Mongolia medi-cine Menggenwusen -Wurile. Methods Column was the Halo C18 HPLC column, mobile phase consisted of acetonitrile -tetrahydrofuran (25∶15)as mobile phase A and 0.1 mol? L-1 of ammonium acetate so-lution as mobile phase B with gradient elution, the volume flow rate was 0.8 mL ? min-1 , column temperature was 30 ℃, detection wavelength was 235 nm.Results The concentration ranges of aconitine, hypaconi-tine, mesaconitine, benzoylaconitine, benzoylhypacoitine and benzoylmesaconine showed good linear relationship in the 3.48 ×10-3 -1.74 ×10-1 , 3.24 ×10 -3 -1.62 ×10-1 , 6.96 ×10 -3 -3.48 ×10-1 , 2.06 ×10 -3-1.03 ×10 -1 ,4.02 ×10 -3 -2.02 ×10 -1 ,6.66 ×10 -3 -3.33 ×10 -1 mg? mL-1 ( r were 0.999 5,0.999 0,0.999 0,0.999 9, 0.999 9, 0.999 9, respectively ) .The average recovery rates were 95.54%, 103.18%, 102.75%, 92.29%, 99.41%, 107.8%, respec-tively.RSDs were 3.70%,1.89%,4.94%,3.67%,4.29%, 3.89%, respectively.Conclusion This method is convenient, accurate and reproducible, applicable for detection of the monoester type alkaloids and diester type alkaloids in traditional Mongolia medicine Menggenwusen-Wurile.