Objective:To investigate the expression and clinical significance of microRNA-30a in renal cell carcinoma (RCC). Methods:The miRNA-30a expression in renal tissues and cell lines was detected using quantitative real-time polymerase chain reac-tion (qRT-PCR), and the relationship between miRNA-30a expression and the clinicopathological features of RCC was analyzed. The effect of miRNA-30a on cancer cells was evaluated using methyl thiazolyl tetrazolium (MTT) assay. In addition, a bioinformatics algo-rithm was adopted to predict the potential targets of miRNA-30a. Results:miRNA-30a was downregulated both in RCC tissues and cell lines. Patients with higher miRNA-30a expression exhibited better prognosis than those with lower miRNA-30a expression. Bioin-formatics algorithm and qRT-PCR both indicated that metadherin (MTDH) was a target of miRNA-30a. Moreover, MTT results showed that miRNA-30a could inhibit cell proliferation. Conclusion:miRNA-30a was inhibited in renal cancer, and low miRNA-30a expres-sion was associated with poor prognosis. In addition, miRNA-30a could inhibit the growth of cells through MTDH.

Aim To study the effects of adenosine triphosphate(ATP) on the proliferation of Chinese esophageal carcinoma Eca-109 and hepatoma SMMC-7721 cells.Methods MTT assay was used to determine the inhibition of the proliferation of the two cultured tumor cell 1ines in vitro by ATP,adenosine(ADO) and uridine triphosphate(UTP).Morphological changes of the two cell lines induced by ATP were observed under light microscope.Results ATP(0.03～0.3 mmol?L~(-1)) and ADO(0.1～0.3 mmol?L~(1)) had inhibitory effects on Eca-109 and SMMC-7721 cells concentration-dependently,and the inhibition by ATP was stronger than that by ADO in both cell lines.For Eca-109 cell line,the maximal inhibition rate of the proliferation by ATP and ADO was 86.36% and 29.88%,and the IC_(50) was 0.056 and 0.823 mmol?L~(-1),respectively.For SMMC-7721 cell line,the maximal inhibition rate of the proliferation by ATP and ADO was 82.06% and 52.84%,and the IC_(50) was 0.218 and 0.517 mmol?L~(-1),respectively.UTP had a very weak inhibitory effect on Eca-109 cell line,with the maximal inhibition rate of 18.27%,and did not significantly affect SMMC-7721 cell line.Exposed to higher concentration(0.3 mmol?L~(-1)) of ATP for 72 h,SMMC-7721 cells displayed morphological changes of apoptosis,but Eca-109 cells did not show the characteristics of apoptosis markedly.Conclusion ATP has a strong inhibitory effect on the proliferation of Eca-109 cell line,which is mainly induced by ATP per se,and a metabolite ADO also has weaker effects.For SMMC-7721 cell line,however,ATP inhibits the cell proliferation mainly via its degradation to ADO.

OBJECTIVE:To observe the clinical efficacy and safety of Kudiezi injection combined with alprostadil in the treat-ment of posterior circulation ischemic vertigo. METHODS:Totally 180 patients with posterior circulation ischemia were randomly divided into group A,group B and group C. All patients were given aspirin,atorvastatin,nutrition nerve,antihypertensive,hypo-glycemic and other conventional treatment. On this basis,group A was given Alprostadil injection 10 μg,adding into 0.9% Sodium chloride injection 100 ml,iv,once a day;Kudiezi injection 30 ml,adding into 0.9% Sodium chloride injection 250 ml,iv,once a day. Group B was given Alprostadil injection(the same usage as group A);group C was given Kudiezi injection(the same usage as group A). The course was 14 d. The clinic data was observed,including clinical efficacy;blood flow velocity in left vertebral ar-tery(LVA),blood flow velocity in right vertebral artery(RVA),blood flow velocity of basilar artery(BA),changes of peak laten-cy(PL)and incubation period latency(IPL)before and after treatment;and the incidence of adverse reactions. RESULTS:The to-tal effective rate in group A was significantly higher than group B and C,with significant difference(P<0.05). After treatment,the blood flow velocity of LVA,RVA and BA in each group were significantly higher than before,group A was higher than group B and C;PL-Ⅰ,PL-Ⅲ,PL-Ⅴ,IPL-Ⅰ-Ⅲ and IPL-Ⅲ-Ⅴ were significantly lower than before,group A was lower than group B and C,with significant differences(P<0.05);however,there were no significant difference between group B and C(P>0.05). There were no obvious adverse reactions during treatment. CONCLUSIONS:Kudiezi injection combined with alprostadil has better effica-cy than only Kudiezi injection or alprostadil in the treatment of posterior circulation ischemic vertigo,with good safety.

P 2 purinoceptors were first subdivided into P 2X and P 2Y subtypes, and later this classification was broadened to include P 2T , P 2Z and P 2D subtypes. In the 1990s, a new kind of receptors was found, which respond to UTP, ATP and ATP?S, but not to 2 MeSATP or ?,? MeATP, this finding led to the definition of the so called “P 2U ” or “nucleotide” receptor. Most recent evidence demonstrated the existence of a pyrimidine receptor responding to UTP but not to ATP. For this case, IUPHAR (International Union of Pharmacology) committee recommended that P 2 purinoceptors be subdivided into P2X and P2Y subtypes, any subtypes of intrinsic ion channel be termed P2Xn, and any subtypes of G protein coupled receptor be termed P2Yn purinoceptors. With the development and application of the molecular biologic technique and the cloning and expression of the receptors, the classification was strongly confirmed.

Objective To study the effect of GTA/AVC dual inhibitors on C6 brain nerve glima cells.Methods MTT method was used to test the brain glioma cells activity of proliferation at different times(12 h,48 h,72 h), and using the flow cytometry to detect the change of cells cycle.Results It has obvious inhibitory effcets on C6 brain nerve glima cells, and it was the most obvious at 48 h.GTA/AVC dual inhibitor(2000 μg/mL) treatment group remarkbly rise the G0/G1 rate of celluar, S-phase cell ratio descend, and the cells apoptosis rate was markedly increased.Compared with the control group and the GTA/AVC dual inhibition (1000 μL/mL)treatment group , were significant difference(P<0.05).Conclusion regulating cells cycle progression.

Pediatric medical traumatic stress is a new research which is based on posttraumatic stress,which is attracting more and more nursing workers' attention.The paper gives a brief introduction of pediatric medical traumatic stress and its related symptom,summarizes the achievement abroad,learns the experience of pediatric medical traumatic stress abroad,provides a reference for nursing workers to have a better understanding of pediatric medical traumatic stress in order to let the sick children receive better treatment.

Parenting stress is regarded as a criterion to evaluate the parent's mentation of children with chronic illness,it not only influence parent's care of children,but also decrease the quality of children's life.Si multaneously,it is a main reason of family imbalance.This paper introduces the definition of parenting stress and evaluation tool summarizes the cunent situation of research in abwad,provides a foundation in order to promote the research of parenting stress among caregivers of children with chronic illness.

After the significance to asses the value of information resources in hospital library was elaborated, the methods to assess the value of information resources in hospital library were constructed by contingent valuation and investment return analysis in combination with the practical service in hospital library according to the analysis of the economic value assessment in domestic and foreign public libraries and academic libraries.

A questionnaire survey was conducted among 319 mental health workers from 319 grassroots health institutions of 21 districts (cities,counties) in Chengdu in March 2013.The items of survey included general information of participants,subjective perceptions and status of community mental health services.Total 252 effective questionnaires were received,the average age of participants was (33.12 ± 9.39) years (19-61);59.5％ had clinical medicine and 16.3％ had public health background;most of them had educational levels of three-year college or below (84.9％);the average duration of working experience was 18 months;86.5％ was part-time;most of participants (87.4％) thought that the mental health service was a hard job and only 25.3％ expressed satisfaction with the work.Only 16.3％ of community health service centers had rehabilitation facilities for mental illness patients.Public health education was carried out for average (2.21 ± 1.62) times/year,the education for mental patients and family members was (1.62 ± 1.83) times/year.Between areas with sufficient detection rate of mental illness and those with insufficient detection rate,there was significant difference in training frequency of mental workers (P =0.012),there were no significant differences in having formal employment of mental workers (P =0.064),professional guidance,co-working with police officers,co-working with Disabled Persons' Federation,and the duration of working experience (all P ＞ 0.05).The results suggest that the capacity of mental health service in community levels is relatively weak in Chengdu,and the personnel training,public education and rehabilitation work of mental health need to be strengthened.

The languages, types and number of digital resources in 8 domestic medical and pharmaceutical college and university libraries were analyzed , which showed that although certain successes have been achieved in devel-opment of digital resources in these libraries, there are some problems needing to be solved, such as weakness in new digital resources development , insufficient integration of free and open access resources , insufficient organiza-tion and revealing of resources .Suggestions were put forward for the solution of these problems , such as optimizing digital resource structure, developing characteristic digital resources, sufficient organizing and revealing digital re-sources.