Abstract?AlM: To study the number of myogenin - positive activated satellite cells in the inferior oblique muscles and the medial muscles of V- pattern exotropia with inferior oblique overaction, to further explore the possible etiological factors of V-pattern exotropia with inferior oblique overaction.? METHODS: The inferior oblique muscles and the medial muscles were cut from V - pattern exotropia patients with inferior oblique overaction during strabismus operation treated as the strabismus group. Cross sections were stained immunohistochemically for the presence of activated satellite cells, as identified by myogenin immunoreactivity. The inferior oblique muscles and the medial muscles were obtained from the corneal transplant donors ( six eyes of six cases) , which treated as the control group.? RESULTS: The frequency of myogenin - positive satellite cells of the inferior oblique muscles was (22. 7± 7.03)% and (4. 2±0. 75)% in the strabismus group and the control group. Significant differences existed in the expression of myogenin in two groups (P<0. 05). Again, the frequency of myogenin-positive satellite cells of the medial muscles was (2. 2±0. 75)% and (4. 5±1. 05)% in the strabismus group and the control group. Significant differences also existed in the expression of myogenin in two groups (P<0. 05).?CONCLUSlON: lt is first report that myogenin-positive satellite cells presents in extraocular muscles of V -pattern exotropia with inferior oblique overaction. The current results suggest that myogenin is one of possible etiological factors of V-pattern exotropia with inferior oblique overaction.

With the worldwide increase in adult-to-adult live donor liver transplantation, more profound basic and clinical medical research has ensued to solve the key problem of graft failure following liver transplantation. The present status of the study of small-for-size liver syndrome was summarized. Its definition, risk factors, pathogenesis,clinical manifestations, prevention and treatment were included.

Dipeptydil-peptidase-4 (DPP-4) inhibitors are designed as oral anti-hyperglycemic drugs for the treatment of type 2 diabetes owing to their effects on the incretin system through sparing incretin hormones including glucagon-like peptide-1 (GLP-1) from rapid degradation by DPP-4.It has been shown that GLP-1 signaling may exert beneficial effects on cardiovascular system.In addition to GLP-1,DPP-4 physiologically cleaves cytokines,chemokines,and neuropeptides involved in inflammation,immunity,and vascular function.It means that DPP-4 inhibitors hold promise for cardiovascular protection independent of GLP-1.And its modulation of endothelial progenitor cells,infiammatory pathway,ischemic response,and lipid metabolism emerges as the major cardiovascular target of DPP-4 inhibitors.

Objective To observe the short-term clinical effect of compound sophora injection combined with Java brucea fruit oil emulsion injection for palliative treatment of advanced malignant tumors. Methods Totally 120 cases of advanced malignant tumor patients were randomly divided into treatment group and control group, each contains 60 cases. The control group was treated with Java brucea fruit oil emulsion, and the treatment group took compound sophora injection additionally by intravenous infusion one time daily. The cycle was 24 d. After 3 cycles, the curative effect, pain relief, improvement situation of quality of life and adverse reactions of the two groups were observed. Results Feasible curative effect evaluation of 112 patients showed:The disease control rate in the treatment group was 89.5% (51/57), and the control group was 74.5% (41/55). The total effective rate of cancer pain relief of the treatment group was 91.2%(52/57), and the control group was 70.9% (39/55). The improvement rate of life quality of the treatment group was 70.2% (40/57), and the control group was 49.1% (27/55). The differences between the two groups was significant (P<0.05). There was no difference in hematology toxicity, liver and kidney function, gastrointestinal adverse reactions and itchy skin incidence between the two groups (P>0.05). And the treatment group had a lower incidence of phlebitis than the control group (P<0.05). Conclusion Compound sophora injection combined with Java brucea fruit oil emulsion for palliative treatment can improve curative effect, significantly improve patients’ quality of life, it was a safe and effective way in treating advanced malignant tumors.

Objective To discuss the effect of triple therapy plus traditional Chinese medicine in the treatment of peptic ulcer (PU) caused by Helicobacter pylori ( Hp) .Methods 148 cases with PU were randomly divided into study group and control group of 74 cases in each group.The control group were treated with triple therapy (omeprazole +clarithromycin +amoxicillin), and the study group were given liver-discharging and heat-clearing and invigorating spleen and removing blood stasis decoction, additionally.Both groups received treatment for one course of 4 weeks.The changes of clinical symptoms, negative conversion ratio of Hp and adverse reaction were compared.Results The scores of clinical symptoms were all significantly decreased in both two groups after treatment (P<0.05), but the study group changed more than control group (P<0.05).The total effective rate was 95.95%, rather higher than 79.73% in control group (P<0.05).The negative conversion ratio of Hp was 82.43%, obviously higher than 64.86% in control group (P<0.05).The incidence of adverse reaction was 12.16% in study group, similar to 9.46% in control group.Conclusion Triple therapy plus traditional Chinese medicine can significantly improve the clinical symptoms, clinical effect and negative conversion ratio of Hp, and has a good safety and tolerability.

Objective To evaluate multi- slice spiral CT angiography (MSCTA) in diagnosing bronchial-pulmonary shunts (BPS). Methods The materials of MSCTA images in 31 patients with DSA-confirmed BPS were retrospectively analyzed. Taking DSA imaging as the reference standard, the consistency in diagnosing BPS at different levels between MSCTA and DSA was compared. Results On MSCTA using aorta-density trigger scanning technique BPS was manifested as wrong-time enhancement of pulmonary artery branches. Wilcoxon test indicated that MSCTA had a high concordance with DSA in detecting BPS occurring at sub-segmental (or above) pulmonary artery branches (Z = -1.854, P > 0.05), but MSCTA detection of BPS occurring at peripheral pulmonary arteries was obviously poorer than DSA (Z = -3.923, P < 0.05). MSCTA confirmed the diagnosis of BPS in 11 cases with a sensitive rate of 35.5% (11/31), and no differences in revealing the number and the shunt levels of BPS existed between DSA and MSCTA. The number of abnormal systemic arteries demonstrated by MSCTA was quite the same as the number detected by DSA. No false positive case was seen. Conclusion MSCTA is very effective in diagnosing high-flow BPS locating at sub-segmental (or above) pulmonary artery branches as well as in revealing abnormal bronchus-related systemic arteries, which is very helpful in preoperatively evaluating the severity of the BP shunting and the supplying arteries to be obstructed.

Objective To investigate the role of CT in the diagnois of superior vena cava syndrome secondary to pulmonary carcinoma. Method Fifty patients with pulmonary carcinoma resulting in SVCS were confirmed by pathological examinations. Relationship between anatomical distribution and gross type of pulmonary carcinoma and modes of pulmonary carcinoma resulting in SVCS were analyzed retrospectively and statistically. Correlation among obstructive degree of SVC, opening of collateral pathway and swelling of chest wall was analyzed retrospectively and statistically. Results For modes of pulmonary carcinoma resulting in SVCS, direct invasion of pulmonary carcinoma was 7 patients, metastasis of lymph node was 16 patients and both of the two was 27 patients.Modes of pulmonary carcinoma resulting in SVCS were different for different gross types of pulmonary carcinoma in different pulmonary lobes ( x2 = 30.012,P ＜ 0.05). On CT appearances of secondary lesions, cases of simple constitution and opening of collateral pathway, simple swelling of chest wall, both of the two and neither of the two were 14 patients, 12 cases, 15 patients and 9 patients in order on CT. With SVC obstruction at different extent,constitution and opening of collateral pathway and swelling of chest wall were different ( x2= 12.881, P ＜ 0.05).Conclusions SVCS resulted from pulmonary carcinoma can be diagnosed by contrast enhanced CT.

Objective:To establish a method for the determination of specnuezhenide in traditional decoction and granules of Zish-enwufa formula, and compare the content between them. Methods:HPLC was used for the determination. The samples were analyzed on a Waters Symmetry C18 column (150 mm × 3. 9 mm,5 μm) with acetonitrile-water (16 ∶84) as the mobile phase. The flow rate was 1. 0 ml·min-1 and the detection wavelength was 224nm. Results: A good linearity of specnuezhenide was within the range of 0.179 2-3.316 μg(r=0.999 9),and the average recovery of berberine hydrochloride was 101.65% with RSD of 1.49%(n=6). Conclusion:The determination method is simple, accurate and sensitive with good reproducibility,which can be used for the determi-nation of specnuezhenide in the preparation. The content of specnuezhenide in the traditional decoction and granules of Zishenwufa for-mula is similar.

30ml) undergoing minimally invasive evacuation of intracranial hematoma in our department were analyzed retrospectively.The chants of IL-1,IL-6 and IL-8 in hematoma fluid were observed continuously.The content of IL-1,IL-6 and IL-8 was determined by radioimmunassay.Results IL-1,IL-6 and IL-8 were observed at 6～12h after hypertensive cerebral hemorrhage,and showed different changes in acute stage.Conclusion Inflammatory cytokines are involved in the pathological process of cerebral hemorrhage.

Objective To explore the pharmacological function of immune enhancer of gypenosides and discuss the dose-effect relationship.Methods The gypenosides was used as an immune enhancer to cure the immune deficit mouse and observe non-specific immunological function by carbon clear test.All of the mice were administrated with CTX and then were divided into six groups:control group,high dose group,middle dose group,lower dose group.Respectively,the carbon clear test was performed in each group and the variation of non-specific immune function was observed.Results The carbon clear test showed that the index of carbon clear test in control group was markedly decreased while the index of carbon clear testing high and middle group was increased.The effect of gypenosides was dose-dependent on the non-specific immune.Conclusion Gypenosides can markedly increase the non-specific immune function.