Objective] To explore both the correlation of matrix metal oproteinase and the imbalance of its tissue inhibitors-MMP-2 and TIMP-2 with glomerulosclerosis and its research progress in traditional Chinese medicine. [Methods] We generalized the research progress in traditional Chinese medicine from such aspects as the biological properties of MMP-2 and TMIP-2 and their mechanisms, their relationship with mesangial proliferative glomerulonephritis and effects of traditional Chinese medicine on their expression by searching the relevant literatures. [Results] Expressions of MMP-2 and TIMP-2 vary in different pathological stages(hyperplasia and sclerosis stage here) of glomerulonephritis, and traditional Chinese herbs could adjust their expressions in different stages. Traditional Chinese herbs can inhibit the development of glomerulosclerosis. [Conclusion] Applying traditional Chinese medicine in the treatment of mesangialproliferative glomerulonephritis could provide the base for further development of TCM as wel as a new choice for treating mesangialproliferative glomerulonephritis.

Objective:To study the effects of hepatitis B virus(HBV)infection in vitro on differentiation of mesenchymal stem cells(MSCs)to liver cells.Methods:MSCs were isolated from human bone marrow by density gradient centrifugation,and expanded by adherent culture.MSCs were cultured under liver-stimulating condition,and different composition of serum was added to the induced medium:Group A:5% fetal bovine serum(FBS);Group B:2.5% FBS+2.5% HBV-containing serum;Group C:2.5% FBS+2.5% serum from healthy volunteers;Group D:the undifferentiated MSCs cultured in LG-DMEM+10% FBS.The expressions of a variety of hepatic lineage markers were analyzed by immunocytochemistry and immunofluorescence.The functionality of differentiated cells was assessed by their ablility to store glycogen.After 2 weeks of exposure to HBV infected serum,HBV-specific protein was also detected by immunocytochemistry.Results:As a result of our hepatic induction,the expressions of albumin(ALB)and alpha-fetoprotein(AFP)by MSCs were observed by immunocytochemical and immunofluorescence techniques.Moreover,MSCs had acquired the ability of glycogen storage which was characteristic of liver cells.Compared with the control group,the proliferation of MSCs was inhibited greatly by the virus-containing serum.After 2 weeks of exposure to HBV infected serum,the surface antigen(HBsAg)was detected in some induced MSCs.However,after immunocytochemical stain for ALB and AFP,there was not much difference between the Group B and C.The ability of glycogen storage of two groups were almost the same.Using confocal microscopy,we found the co-expressions of ALB and HBsAg in the same differentiated cells.Conclusion:The bone marrow MSCs have the ability to trans-differentiate into functional hepatocyte-like cells,hence may serve as a cell source for tissue engineering and cell therapy of hepatic diseases.HBV infected serum could inhibit the proliferation of MSCs in culture,but it seemed that the hepatic differentiation of the cell was unsuppressed.

Pediatric hypertrophic cardiomyopathy (PHCM)is a heterogeneous heart muscle disorder which is characterized by unexplained ventricular hypertrophy.Successful determination of a specific diagnosis is likely to have significant impact on management and survival because of the differences in etiology-specific sur-vival.This review provides an overview of PHCM with a focus on its etiology and their heart features.

Although hemopoietic stem cell transplant have been used in treating malignant diseases of hematological system using high-dose chemotherapy and radiotherapy before transplant or non-transplant is very important. When the dose of chemotherapy and radiotherapy exceeds the ability the tissues can bear, normal tissue can be badly damaged, which limits the therapeutic efficacy of chemotherapy and radiotherapy. Treatments of hematological malignancies are expected to achieve the best therapeutic efficacy and the least damage of normal cells and tissues. Amifostine is an inorganic thiophosphate eytoprotective agent,its chemname is S-2(3-aminopropyl) amino dihydrogen phosphate.h is a prodrug whose active constitutent is free thiol,it can clears free radicals produced from cytotoxic drugs and radiotherapy in tissues exposed to them. It is a broad-spectrum cytoprotective agent which can protect multifarious normal tissues against damage of chemotherapy and radiotherapy. Using amifostine in proconditioning of hemopoietic stem cell transplant can lessen hematological and renal toxicity, it can also lessen renal toxicity produced by chemotherapeutics as platinum,cyclophosphamide and vincristine. The main purpose of this review is to discuss the cytoprotection of amifostine in hematological malignancies.

Brachytherapy has become an important treatment for soft tissue sarcoma by its unique radio-biology and radiation physics characteristics, the treatment time is divided in accordance with preoperative brachytherapy, intraoperative brachytherapy, postoperative brachytherapy, brachytherapy alone, which in im-proving the rate of limb preservation and local control rate sarcoma has played an important role. However, the timing of treatment, treatment modalities, the choice of implantation dose is still in dispute.

The article sums up the experience of applying multimedia technology to Prosthodontics teaching and analyzes its necessities and problems to improve the teaching quality.

An introduction to the concept ofintegration of medical service and aging care,the strategic goals and policies in support of the new ecology of integration of medical service and aging care.The authors described specific practices in the development of laws and regulations,provision of various services and update means to build such an ecology in Wuxi,along with proposals to speed up the innovation and development.

Objective To explore job satisfaction degrees and the influencing factors among community nurses. Method General information questionnaire and the revised job satisfaction questionnaire were adopted in the investigation among 124 nurses from 28 community health service centers in Nanchong City between February to October 2016. Results The total score was (119.62 ± 15.37), at the moderate to high level. Of the eight dimensions, relationship with colleagues was scored the highest, and personal growth was the lowest. The influencing factors of the nurse's job satisfaction were found to be employment type, work positions, service duration in the community and whether they have been trained on community nursing position (all P<0.05). Conclusions The job satisfaction degree of community nurses is generally at middle level. Therefore, measures should be taken to improve the job satisfaction for the development of community nursing services.

ObjectiveTo discuss the clinic application and surgical technique of the free flap by inverted-Y-shape microvascular anastomosis in tissue defect repair and functional reconstruction of the limb.Methods Twenty-two flaps in 18 patients were involved in the study between June 2006 and September 2010( 12 cases for soft tissue defect repair with exposure of the tendons, bones or joints, and 6 cases for functional reconstruction;five cases were around the elbows and 13 cases were around the extremity of the lower limb). Before free flap graft, twelve cases with infective tissue defect were debrided extensively and covered with vacuum sealing drainage from 1 to 2 times and each time last 5 to 7 days until the granulation tissue growing well.Designed the free anterolateral thigh flaps with the transverse and descending branches of the lateral femoral circumflex vessels or free latissimus dorsi flaps with the subscapular and circumflex scapular vessels forming a inverted-Y-shape pedicle to repair the tissue defect or to reconstruct the limb function.The recipient artery was sectioned and the arterial tree of the flap was anastomosed to the recipient vessel by two end-to-end anastomoses.This inverted-Y-shape microvascular anastomosis could supply blood for both the free flap and the extremity of the limb.Not all of the veins of the recipient limb need to be cut off.Observed the circulation of the limb extremity, and evaluated the quality of flaps'survival.ResultsComplete flap survival was achieved in 21 flaps and without vaso-occlusive crisis;while partial flap loss in 1 case, which healed after changing dressings.The mean follow-up was 16.2 (6-36) months postoperatively,appearance of the flaps and the functions of limbs were satisfactory,and no obvious complication was found in the donor site.ConclusionThe free flap by inverted-Y-shape microvascular anastomosis is a new choice for the tissue defect repair and functional reconstruction of the limb,especially for the wound around elbow or extremity of the lower limb with one major artery destroyed.