On white rats, high doses of paracetanol (2g/kg of body mass) and carbonetetrachloride (CCl4 –1.4ml/kg body mass administrated orally) increased dramatically serum transaminase AST and ALT, reduced liver capacity of cholesterol and total protein synthetizations. Granulous degeneration, lipid degeneration and necrosis focus were observed pathohistologically. Linh chi mushroom fluid extracts with the dose of 4g/kg of body mass reversed these biochemical indicators and restored nearly normally the damaged tissues. Similar results were obtained with the oral dose of 25mg Silimarin brand name preparation/kg body mass.
Agaricales ; Reishi ; Liver ;

Background: According to traditional medicine, Ganodermataceae has many good effects on such as anti-oxidant, liver protection, sedation, anti-tumor, treatment of dyslipidemia,... Hong chi DaLat \ufffd?[Ganoderma lucidum (HC)] is a species of Ganodermataceae. This species has two strains DL1 and DL2, was grown and used mainly in Da Lat but they had not been fully studied. Objectives: To evaluate the cholesterol lowering effect of HC in cholesterol induced hypercholesterolemic rats. Subjects and method: Rats were given orally the cholesterol 10% with 0.5g/kg body weight/day during 6 consecutive weeks in order to induce hypercholesterolemic rats. The hypocholesterolemic effect of extract of (HC) was investigated at the dose of 4g/kg administered orally in hyperlipidaemic rats after 6 weeks. Results: HC with the dose of 4g/kg significantly decreased plasma triglyceride, total cholesterol, LDL \ufffd?C levels by 24.82%, 27.41% and 54.32% respectively, but increased HDL \ufffd?C by 53.34%. The effect of HC on plasma cholesterol levels with the oral dose of 4g/kg, 12g/kg was equal to each other and to that of cholestyramin at the dose of 1.6g/kg. Conclusion: HC has hypocholesterolemic effect in rats.
Reishi ; Hyperlipidemias ; Rats ;

Through the comparative study on the appearance characters and internal structure of cultivated and wild Ganoderma lucidum in Huoshan,this paper provides a reference for the further study of G. lucidum. In this study,the similarities and differences between cultivated G. lucidum " Huozhi No. 1" and wild G. lucidum in Huoshan were compared by means of character observation,optical microscopy and scanning electron microscope( SEM). The results showed that the pileus color of " Huozhi No. 1" was yellowish brown and thicker,while that of wild G. lucidum was mainly reddish brown,the context was thinner,and there were gravel and rotten wood at the bottom of the stipe. A clear skeletal hyphae and binding hyphae were observed in cultivated and wild G. lucidum,but there was no significant difference. The shell layer,context layer,mediostratum layer and spores of cultivated and wild G. lucidum were observed by SEM,and the results showed that there was no significant difference. It was found that the mediostratum of " Huozhi No. 1" was thin and irregular,while the mediostratum of wild G. lucidum was neat and compact. There were two types of spores in wild G. lucidum,one of which retained the outer wall of spore type Ⅰ,with tiny pores on the surface. The other is type Ⅱ spores with many spinous processes on the surface,which may be formed by type Ⅰ spores falling off the outwall. In this study,the appearance characters and internal structure of cultivated and wild G. lucidum in Huoshan were systematically observed and compared,which provided theoretical basis and reference for the identification and quality evaluation of cultivated and wild G. lucidum.
Ganoderma ; Hyphae ; Reishi

The mycelia obtained from somatic tissues (basidiocarps) and basidiospores were irradiated with gama rays from 60Co - source and +5C from synchrotron AVF. The LG50 doses were determined >=100 Krad and =50 Krad, respectively. The fruitful cultivations from fragmenting inoculate were carried out for screening and selections of new mutants with high yields and rich in bioactive substances.
Antineoplastic Agents ; Reishi ; Drugs, Chinese Herbal ; Radiation

The oil content and fatty acid composition of Ganoderma lucidum collected from different producing areas, varieties, tissue types and growth periods were measured and analyzed. The results showed that the oil content was 23. 61%-34.17% in different domestic producing areas of China; the oil content of fruiting bodies from major varieties cultured in Zhejiang province were 0.81%-1.87%, wall-unbroken spores were 0.07%-0.24%, wall-broken spores were 27.54%-34.17%, so the oil content of wall-unbroken spores were much higher than fruiting bodies, and wall-breaking treatment would increase the oil extraction rate 150-340 times. G. lucidum spores oil was mainly composed of unsaturated fatty acid composition. oleic acid and linoleic content were 53.26%-58.16% and 10.69%-16.87% respectively. Fatty acid composition ratio of spores and fruiting bodies were significantly different by PLS-DA. Determining the composition of fatty acid, especially the content of oleic acid, stearic acid and palmitic acid, could identify the tissue types of G. lucidum products' sources. In addition, the study result showed that the spores and fruiting bodies collected in the first year contained richer oil and fatty acid than second year's samples from the same variety of G. lucidum.
Fatty Acids ; analysis ; Oils ; analysis ; Reishi ; chemistry

Ganoderic acid(GA) is one of main bioactive components produced by Ganoderma lucidum,which a traditional Chinese herbal medicine and a kind of tracyclic triterpene lanosterol derivatives with highly oxidized structure. It has a variety of important pharmacological activities,such as anticancer,immunoregulation,anti-oxidation,anti-diabetes and anti-HIV. At present,the studies of GA mainly focus on biosynthesis,fermentation control,isolation and purification,structure identification and pharmacological effects.However,there are a fewer pharmacokinetic studies of GA,although it is closely related to the clinical application. Recent studies have shown that GA can be absorbed rapidly by gastrointestinal tract and distributed in various tissues and organs after oral intake. GA is metabolized by liver at phase Ⅰ and phase Ⅱ,and then mainly excreted by bile. In this paper,the pharmacokinetic characteristics of GA and its absorption,distribution,metabolism and excretion(ADME) will be systematically summarized,in order to provide scientific basis for the application and development studies of Ganoderma triterpenoid drugs and their rational clinical use.
Humans ; Lanosterol ; pharmacokinetics ; Reishi ; chemistry ; Triterpenes ; pharmacokinetics

A Monokaryotic strain G8M without clamp connections was isolated from germinated basidiospore that was obtained from cultivated fruit body. Strain G8M was used as a tester isolate for 'dikaryon-monokaryon mating' (di-mon mating) with the strains of Ganoderma lucidum, G6 and G35 (Korean wild strains), G3 (Taiwan), G4 (Canada), G15 (America), G. oregonense G24, G. resinaceum G28, G. oerstedii G23, and G. subamboinense G29. Isolate G8M was compatible to Korean strains G6 and G35, but was incompatible to foreign strains G3, G4, or G15. Compatible reactions between strains were readily observed macroscopically. Clear barrage lines formed between incompatible strains. These clear lines were not apparent in compatible di-mon matings. The Korean strains were morphologically distinct; they did not form any chlamydospores, and stopped growth at 35degrees C. The strains of G. lucidum from Korea may be considered as different species from Taiwan, Canadian and American cultures.
Classification* ; Fruit ; Ganoderma* ; Korea ; Reishi* ; Taiwan

The effects of the composition of a mixture containing food waste compost (FWC), rice bran (RB), and oak sawdust (SD) on the antler-type fruiting body (FB) yield of Ganoderma lucidum were studied. Experiments were performed using 0 (control), 5, 10, 15, 20, 25, 30, 35, and 40% (w/w) FWC added to a basal growth medium consisting of 20% (w/w) RB and 80% (w/w) SD. The content of 15% FWC gave the highest FB yield (27.0 +/- 1.3 g/bottle), which was 44% higher than the yield (18.6 +/- 2.8 g/bottle) of the control treatment. However, FWC contents of 20~40% showed reduced yield (2.4~23.0 g/bottle), partly because FWC had a high Na concentration (0.6%). These results demonstrate the potential for use of FWC as a component of a growth medium for production of G. lucidum FBs.
Drugs, Chinese Herbal ; Fruit ; Ganoderma ; Reishi ; Soil

The clinical aspects of intoxication can be similar to those of drug hypersensitivity syndrome, including desquamation, hematologic abnormalities, multiple organ failure, and even death. Many people consume mushrooms that they collect in the mountains, and consequently, the incidence of poisonous mushroom intoxication is increasing. Podostroma cornu-damae is a poisonous mushroom not well-known to the public that contains trichothecene, a deadly poison. It is difficult to differentiate P. cornu-damae from other edible mushrooms, especially young Ganoderma lucidum. Therefore, the general public should be aware of the dangers of indiscreet ingestion of mushrooms. When physicians treat patients presenting clinical symptoms similar to those of drug hypersensitivity reaction following the intake of mushrooms, poisonous mushroom intoxication must be taken into consideration. We report this case together with a literature review, as we experienced one P. cornu-damae intoxication case showing similar clinical aspects to drug hypersensitivity syndrome.
Abnormalities, Multiple ; Agaricales ; Drug Hypersensitivity ; Eating ; Humans ; Incidence ; Reishi

PURPOSE: To investigate the effect of aqueous extract of Ganoderma lucidum(G.I.) on the survival of tumor cells in vitro and on the growth of tumors in vivo. MATERIALS AND METHODS: Dried G.I. was made into powder, extracted with distilled water, filtered and diluted from a maximum concentration of 100 mg/ml in sequence. The cytotoxicity of G.O. in vitro was evaluated from its ability to reduce the clonogenicity of SCK tumor cells. For the tumor growth delay study, about 2' 105 of SCK tumor cells were subcutaneously inoculated in the legs of A/J mice. The first experimental group of mice were injected i.p. with 0.2ml of 250 mg/kg of G/I. From the first day after tumor inoculation for 10 days. The second experimental group of mice were injected i.p. with 0.2ml of 250 mg/kg of G.I. either once a day for 10 days or twice a day for 5 days beginning from the 7th day after tumor inoculation. RESULTS: 1. Cytotoxicity in vitro; survival fraction, as judged from the curve, at G.I. concentration of 0.5,1,5,10,25,50 and 100 mg/ml were 1.0, 0.74+/-0.03, 0.18+/-0.03, 0.15+/-0.02, 0.006+/-0.002, 0.015 and 0.0015, respectively. 2. Tumor growth delay in vivo; a) the time required for the mean tumor volume to grow to 1,000mm3 was 11 days in the control group and 14 days in the experimental group. b) the time required for tumor volume to increase 4 times was 11 days in the control group while it was 10.5 and 12 days in the groups injected with G.I. once a day and twice a day from the 7th day after tumor inoculation respectively. CONCLUSION: Aqueous extracts of G.I. showed a marked cytotoxicity on the SCK mammary cells in vitro. Tumor growth delay was statistically significant when G.I. injection was started soon after tumor inoculation, but it was not significant when injection was started after the tumors were firmly established.
Animals ; Ganoderma* ; Leg ; Mice ; Reishi* ; Tumor Burden ; Water