1.Purification of glutathione-S-transferase fusion protein by glutathione coupled magnetic particles.
Jingjing ZHU ; Liu YANG ; Lei YANG ; Chao CHEN ; Yali CUI
Chinese Journal of Biotechnology 2009;25(8):1254-1260
We established a purification system for glutathione-S-transferase (GST) fusion protein using glutathione coupled magnetic particle. Glutathione was coupled covalently to the surface of magnetic particles with isothiocyanate functional groups. Cell lysate, containing the fusion protein, was then incubated with these glutathione coupled magnetic particles at room temperature. Unbound and non-specifically bound proteins were removed by wash steps. Subsequently, the GST-fusion protein was eluted from the magnetic particles by the addition of reduced glutathione. The resulting fusion protein was tested for purity using SDS-PAGE and demonstrated by Western blotting. The concentration of the fusion protein was measured by Bradford method. Both the conditions for incubation and washing were optimized. The results showed that 150 microg glutathione could be bound on 1 mg of particle surface and 10 mg of the glutatione-coupled magnetic particles was suitable for 100 microL lysate, the optimal incubation time for reaction between particles and lysate was 40 min. The magnetic particles could help purify efficiently GST-fusion protein with a yield of around 516 microg fusion protein per 10 mg particles. Magnetic particles can be successfully used in a simple, rapid and reliable method for the purification of GST-fusion proteins.
Glutathione
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chemistry
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Glutathione Transferase
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chemistry
;
isolation & purification
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Magnetite Nanoparticles
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chemistry
;
Protein Binding
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Recombinant Fusion Proteins
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chemistry
;
isolation & purification
2.Refolding of the fusion protein of recombinant enterokinase light chain rEKL.
Chinese Journal of Biotechnology 2006;22(5):811-815
The fusion protein of enterokinase light chain, DsbA-rEKL, was expressed mainly in inclusion body in E. coli. The recombinant bacteria was fermented to high density, with high expression of the fusion protein. After being washed with 0.5% Triton X-100 and 4mol/L urea, the inclusion body was dissolved in 6mol/L guanidine and 100mmol/L DTP, derivatized by cystine and refolded by pulse refolding. The strategy of pulse refolding involved the addition of 0.03mg/mL of fusion protein until its final concentration reached 0.3mg/mL. The refolded protein was autocleaved and the active EKL molecule was released after adding 2mmol/L CaCl2. Using the two-step purification processes of IDA-Sepharose chromatography and Q-Sepharose chromatography, the purity of rEKL was found to be above 95%, with a high activity to cleave the recombinant reteplase fusion protein Trx-rPA. The yield of purified rEKL was more than 60mg/L of cultures. As a result, the therapeutic proteins like rPA could be produced on a large-scale in a way such as expressed in the form of fusion proteins.
Enteropeptidase
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chemistry
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Escherichia coli
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genetics
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Protein Folding
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Recombinant Fusion Proteins
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chemistry
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isolation & purification
3.Refolding and purification of the huGM-CSF(9-127)-IL-6(29-184) fusion protein.
Qiang-Ming SUN ; Hong-Yan LIU ; Chang-Bai DAI ; Yan-Bing MA ; Mao-Sheng SUN ; Wei-Ming XU
Chinese Journal of Biotechnology 2002;18(3):291-294
The huGM-CSF(9-127)-IL-6(29-184) fusion protein was precipitated on column when being purified by Q Sepharose H.P. ion exchange chromatography after renaturation by dilution. To solve this problem, a novel purification and refolding strategy was adopted. Inclusion bodies was first purified by Q Sepharose H.P. ion exchange in 8 mol/L urea, followed by in situ refolding on column by Sephacryl S-200. Renatured fusion protein was obtained in a purity of more than 95%. It was showed that the method of refolding on gel filtration column is efficient, with relative refolding rate at 80%. By the whole procedure, refolding and purification of recombinant protein can be performed within one day. This strategy is also promising to be applied in large scale purification and refolding of recombinant protein from inclusion bodies in E. coli.
Granulocyte-Macrophage Colony-Stimulating Factor
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chemistry
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isolation & purification
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Interleukin-6
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chemistry
;
isolation & purification
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Peptide Fragments
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chemistry
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isolation & purification
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Protein Folding
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Recombinant Fusion Proteins
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chemistry
;
isolation & purification
4.Expression and purification of heptad repeat region of the mumps virus F protein and analysis of characteristics.
Yue-Yong LIU ; Ming-Guang FENG ; Jie-Qing ZHU ; Li-Jie JIANG ; Po TIEN
Chinese Journal of Biotechnology 2004;20(3):377-381
Two Heptad repeat motifs (HR1 and HR2) from paramyxoviruses F protein could form thermostable heterodimers containing high alpha-helix while virus infected host cell. Following that the viral membrane and the host cell membrane were juxtaposed, which leads to membrane fusion. Mumps virus (MuV) is a member of the genus Rubulavirus in the family of Paramyxoviridae. MuV could use similar infection mechanism as well as other paramyxoviruses. In this study the HR1 and HR2 regions of MuV F protein were predicted by a computer program and expressed in E. coli with the GST fusion expression system. The GST fusion or GST-removed proteins were purified with Gluthathion Sepharose 4B Column. GST pull-down experiment suggested the interaction of HR1 and HR2 peptides, and analysis of gel filtration showed two peptides could form multimer, which indicates that the HR regions of MuV F protein may play an important role in virus fusion.
Membrane Fusion
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genetics
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Mumps virus
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genetics
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Recombinant Fusion Proteins
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biosynthesis
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chemistry
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genetics
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isolation & purification
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Repetitive Sequences, Amino Acid
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Viral Fusion Proteins
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biosynthesis
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genetics
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isolation & purification
5.Preparation of metal chelate affinity chromatographic medium and its application in the purification of 6 x histidine-tagged protein.
Shu-Juan LI ; Yong-Liang SUN ; Dao-Dao HU ; Chao CHEN ; Ya-Li CUI
Chinese Journal of Biotechnology 2007;23(5):941-946
Using Sepharose CL-6B as support, 3-Chloro-1, 2-epoxypropane as activated agent, carboxymethylated aspartate (CM-Asp) as chelating ligand, A chelate affinity chromatographic medium based on Co2+, named Co-CM-Asp-Sepharose, was prepared and used to purify 6 x His-tagged fusion proteins. The amount of Co-CM-Asp-Sepharose reacted with 200 microL of lysate, the incubation time, wash condition and the imidazole concentration in the elution buffer were optimized. The purification results using Co-CM-Asp-Sepharose and Ni-NTA-Agarose (product of Qiagen) were compared. The CD155D1 fusion protein was also purified from 5mL of lysate and the amount of protein was determined by Bradford method. The results show that 60 microL of Co-CM-Asp-Sepharose (50% suspension) was suitable for the protein purification from 200 microL of lysate, the optimal incubation time of medium and lysate was 30 min, the optimal imidazole concentration in the eluting buffer was 200 mmol/L, and 200 microg of fusion protein was obtained. In a big scale experiment, 4.6 mg of fusion protein was obtained from 5 mL of lysate using 1.5 mL of Co-CM-Asp-Sepharose (50% suspension). Compared with Ni-NTA-Agarose, the Co-CM-Asp-Sepharose medium exhibits higher selectivity and the protein possesses higher purity.
Aspartic Acid
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chemistry
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Chelating Agents
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chemistry
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Chromatography, Affinity
;
methods
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Epoxy Compounds
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chemistry
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Histidine
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biosynthesis
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chemistry
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genetics
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Polymers
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chemistry
;
Recombinant Fusion Proteins
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isolation & purification
;
Sepharose
;
chemistry
6.The renaturation and purification of RGD-staphylokinase by gel filtration.
Ao CHENG ; Gang SONG ; Hua-Bo SU ; Min YU ; Yu-Yang LI ; Hou-Yan SONG
Chinese Journal of Biotechnology 2002;18(6):693-697
A recombinant RGD-Staphylokinase(RGD-Sak) with thrombolytic and anti-thrombolytic bifunction was expressed in E. coli. The expression product accumulates as inclusion bodies. In order to obtain active molecule, the RGD-Sak in the inclusion body should be denatured and then renatured. The renaturation of RGD-Sak was performed by gel filtration. Comparing with the traditional way of dilution renaturation, gel filtration way is better than the traditional one, since there are some advantages, such as simple processing, high recovery, low cost and higher purity after renaturation, After renaturation, RGD-Sak was purified by Q-Sepharose FF, and the purity was more than 95%. Analysis of CD spectra showed that the final product from the two renaturation ways have similar CD spectra. It was demonstrated that RGD-Sak molecules proceeded correct refolding through gel filtration or dilution renaturation process.
Chromatography, Gel
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Circular Dichroism
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Metalloendopeptidases
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chemistry
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isolation & purification
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Oligopeptides
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isolation & purification
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Protein Folding
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Protein Renaturation
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Recombinant Fusion Proteins
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chemistry
;
isolation & purification
7.Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system.
Xing-Guo GONG ; Jing JI ; Jie XIE ; Yuan ZHOU ; Jun-Yan ZHANG ; Wen-Tao ZHONG
Journal of Zhejiang University. Science. B 2006;7(1):13-19
v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl beta-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression.
Bacterial Proteins
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biosynthesis
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chemistry
;
genetics
;
isolation & purification
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Glutathione Transferase
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biosynthesis
;
genetics
;
isolation & purification
;
Oncogene Protein pp60(v-src)
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biosynthesis
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chemistry
;
genetics
;
isolation & purification
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Protein Engineering
;
methods
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Recombinant Fusion Proteins
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biosynthesis
;
chemistry
;
isolation & purification
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Saccharomyces cerevisiae
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genetics
;
metabolism
8.Preparation of anti-hCG antibody-like molecule by using a RAD peptide display system.
Mengwen LIU ; Mei WANG ; Qiong WANG ; Huawei XIN
Chinese Journal of Biotechnology 2019;35(5):871-879
By using an RAD peptide display system derived from the ATPase domain of recombinase RadA of Pyrococcus furiosus, an anti-hCG antibody-like molecule was prepared by grafting an hCG-binding peptide to the RAD scaffold. After linking to sfGFP gene, a gene of hCG peptide-grafted RAD was synthesized and cloned into a bacterial expression vector (pET30a-RAD/hCGBP-sfGFP). The vector was transformed into Escherichia coli, and expression of the fusion protein was induced. After isolation and purification of the fusion protein, its binding affinity and specificity to hCG were determined by using a process of immunoabsorption followed by GFP fluorescence measurement. A comparison of hCG-binding activity with a similarly grafted single-domain antibody based on a universal scaffold was performed. The measurement of hCG-binding affinity and specificity revealed that the grafted RAD has an optimally high binding affinity and specificity to hCG, which are better than the grafted single-domain antibody. Moreover, the affinity and specificity of grafted RAD molecule are comparable to those of a commercial monoclonal antibody. In addition, the hCG-binding peptide-grafted RAD molecule has a relatively high biochemical stability, making it a good substitute for antibody with potential application.
Antibodies, Monoclonal
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chemistry
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isolation & purification
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metabolism
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Antibody Specificity
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DNA-Binding Proteins
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genetics
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metabolism
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Escherichia coli
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genetics
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Escherichia coli Proteins
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metabolism
;
Humans
;
Peptides
;
Recombinant Fusion Proteins
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genetics
;
metabolism
9.Extraction and purification of NUDT9 homology domain of human transient receptor potential melastatin 2 channel.
Peiwu YE ; Xiafei YU ; Cheng MA ; Wei YANG
Journal of Zhejiang University. Medical sciences 2019;48(1):5-11
OBJECTIVE:
To develop methods of extraction and purification of Cterminal NUDT9 homology domain of human transient receptor potential melastatin 2 (TRPM2) channel.
METHODS:
After sonication and centrifuge of strain Rosetta (DE3) which was induced by isopropylthio-β-D-galactoside, GST-NUDT9-H was collected after the binding of supernatant with GST beads and eluted with reduced glutathione. Then the elution buffer containing fusion protein was purified by size exclusion chromatography after concentration and centrifuge. Finally, with the cleavage of thrombin and binding with the GST beads, NUDT9-H with high purity in supernatant was collected.
RESULTS:
The GST-NUDT9-H fusion protein was stabilized with lysis buffer containing 0.5% n-dodecyl -β-d-maltoside (DDM), and wash buffer containing 0.025% DDM in size-exclusion chromatography system, and finally the NUDT9-H with high purity was obtained after cleaved by thrombin (1 U/2 mg fusion protein) for 24 h.
CONCLUSIONS
Due to the poor stability of NUDT9-H, it is necessary to add DDM in extraction and purification buffer to stabilize the conformation of NUDT9-H, so as to increase its yields and purity.
Escherichia coli
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genetics
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Glucosides
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chemistry
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Humans
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Protein Domains
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Protein Stability
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Pyrophosphatases
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chemistry
;
genetics
;
isolation & purification
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Recombinant Fusion Proteins
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chemistry
;
isolation & purification
;
TRPM Cation Channels
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chemistry
;
isolation & purification
;
Thrombin
;
metabolism
10.Preparation of rhIL-11 from fusion protein by using enterokinase.
Journal of Experimental Hematology 2008;16(4):903-909
The study was aimed to investigate the technological parameters of rhIL-11 preparation from fusion protein by using enterokinase. Fusion expression vector pET32a/IL-11 was transferred into E.coli BL21 (DE3) pLysS and its expression was induced by IPTG, the lysis supernatant of the engineering strain was purified by Ni-NTA resin and then the target rhIL-11 was digested by auto-cleavaged DsbA-EKL-(His)(8). The results showed that after affinity chromatography, Trx-IL-11 was obtained with the yield of 11.25mg/g, the purity of 89.2% and the recovery of 91.8%. Finally the target rhIL-11 was digested and purified to over 95%. In conclusion, the preparation method of rhIL-11 from fusion protein by using enterokinase is simple and feasible with good separation, which can meet industrial requirements.
Enteropeptidase
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chemistry
;
genetics
;
metabolism
;
Escherichia coli
;
genetics
;
metabolism
;
Genetic Vectors
;
genetics
;
Humans
;
Interleukin-11
;
biosynthesis
;
genetics
;
Protein Engineering
;
methods
;
Recombinant Fusion Proteins
;
biosynthesis
;
genetics
;
isolation & purification