1.Evaluation of the Role of 5-Hydroxytryptamine Receptor Subtypes in the Regulation of Nociceptive Transmission in the Rat Spinal Cord.
Chang Young JEONG ; Hong Buem BAE ; Hun Chang PARK ; Jeong Il CHOI ; Myung Ha YOON
Korean Journal of Anesthesiology 2004;47(6):856-861
BACKGROUND: Spinal 5-hydroxytryptamine (5-HT) has been shown to display an antinociceptive effect, which is mediated by 5-HT receptors. Previous studies have revealed the presence of at least four types of 5-HT receptors in the spinal cord. The aim of this study was to assess the role of each spinal 5-HT receptor in the antinociception of intrathecal 5-HT using the formalin test. METHODS: Rats were implanted with lumbar intrathecal catheters. After the administration of 5-HT, formalin-induced nociceptive behavior (flinching response) was observed for 60 min. To further clarify the role of the 5-HT receptors in the antinociception of 5-HT, several antagonists of 5-HT receptors were administered intrathecally 10 min before 5-HT delivery, and formalin was injected 10 min later. RESULTS: Intrathecal 5-HT dose-dependently suppressed flinching during phase 1 and 2 in the formalin test. 5-HT1B (GR 55562), 5-HT2C (N-desmethylclozapine), 5-HT3 (LY-278,584) and 5-HT4 (SDZ-205,557) receptors antagonists reversed this antinociception by 5-HT during both phases in the formalin test. 5-HT1A receptor antagonist (WAY-100635) decreased antinociception by 5-HT in phase 2, but not in phase 1. A 5-HT1D receptor antagonist (BRL 15572) did not antagonize the antinociception of 5-HT in either phases. CONCLUSIONS: Spinal 5-HT1B, 5-HT2C, 5-HT3 and 5-HT4 receptors, but not the 5-HT1D receptor, are involved in the antinociception of serotonin in the facilitated state and in the acute pain evoked by a formalin stimulus. The 5-HT1A receptor seems to play a role in 5-HT-induced antinociception in the facilitated state.
Acute Pain
;
Animals
;
Catheters
;
Formaldehyde
;
Pain Measurement
;
Rats*
;
Receptor, Serotonin, 5-HT1A
;
Receptor, Serotonin, 5-HT1D
;
Receptors, Serotonin
;
Receptors, Serotonin, 5-HT4
;
Serotonin*
;
Spinal Cord*
2.New Generation Laxatives.
Sung Noh HONG ; Jeong Eun SHIN ; Kyoung Sup HONG ; Kee Wook JUNG ; Tae Hee LEE ; Bong Eun LEE ; Sun Young PARK ; Seong Eun KIM ; Kyung Sik PARK ; Suck Chei CHOI
Korean Journal of Medicine 2015;88(1):9-14
A significant proportion of chronic constipation patients are dissatisfied with their treatment. Recently, a number of new medications have been introduced for patients refractory to conventional laxatives, such as prucalopride, lubiprostone, linaclotide, and elobixibat. Prucalopride is a novel gastrointestinal prokinetic agent that acts as a 5-hydroxytryptamine type 4 (5-HT4) agonist. Compared with older nonselective 5-HT4 agonists, the higher selectivity of prucalopride for 5-HT4 receptors can reduce the risk of significant adverse cardiovascular events. Prucalopride improves stool frequency and consistency, and reduces the need for rescue medications. Lubiprostone, a chloride channel activator, increases the secretion of intestinal fluid, improves the stool frequency and consistency, and reduces straining. Linaclotide, a guanylate cyclase-C agonist, is effective in treating patients with chronic constipation and its effect on visceral sensitivity, as shown mainly in animal studies, provides an attractive pharmaceutical option for patients with irritable bowel syndrome with constipation. Elobixibat is an ileal sodium-dependent bile acid transporter inhibitor that blocks the enterohepatic circulation of bile acids, increasing the bile acid concentration in the intestine, which accelerates colonic transit and softens the stool. A phase III trial of the treatment of chronic constipation and irritable bowel syndrome with constipation is underway. The clinical application of new-generation laxatives will contribute to the management of chronic constipation refractory to conventional laxatives.
Animals
;
Bile
;
Bile Acids and Salts
;
Chloride Channels
;
Colon
;
Constipation
;
Enterohepatic Circulation
;
Humans
;
Intestines
;
Irritable Bowel Syndrome
;
Laxatives*
;
Receptors, Serotonin, 5-HT4
;
Serotonin
;
Serotonin 5-HT4 Receptor Agonists
;
Lubiprostone
3.Characteristics of 5-Hydroxytryptamine Receptors Involved in Contraction of Feline Ileal Longitudinal Smooth Muscle.
YiYi WANG ; Sun Young PARK ; Kyung Hoon OH ; Youngsil MIN ; Yun Jeong LEE ; Seok Yong LEE ; Uy Dong SOHN
The Korean Journal of Physiology and Pharmacology 2011;15(5):267-272
A number of studies have demonstrated that 5-hydroxytryptamine (5-HT) can induce muscle contraction or relaxation response and enhance secretion in the gastrointestinal tract via a multiplicity of 5-HT receptor subtypes. In the present study, we investigated the pharmacological characterization of the 5-HT-induced contractile response in longitudinal smooth muscle isolated from the feline ileum. Addition of 5-HT into muscle chambers enhanced the basal tone and spontaneous activity in a concentration-dependent manner. The neurotoxin tetrodotoxin did not alter the 5-HT-induced contraction of the longitudinal muscles. Neither atropine nor guanethidine affected the contraction. The 5-HT agonists, 5-methylserotonin hydrochloride and mosapride, also evoked concentration-dependent contractions. The 5-HT-induced contraction was enhanced by the 5HT2 receptor antagonist ketanserin and the 5-HT3 receptor antagonist ondansetron but was inhibited by the 5-HT1 receptor antagonist methysergide and 5-HT4 receptor antagonist GR113808. These results indicate that 5-HT1 and 5-HT4 receptors may mediate the contraction of the 5-HT-induced response and 5-HT2 and 5-HT3 receptors may mediate 5-HT-induced relaxation in feline ileal longitudinal smooth muscles.
Atropine
;
Benzamides
;
Contracts
;
Gastrointestinal Tract
;
Guanethidine
;
Ileum
;
Indoles
;
Ketanserin
;
Methysergide
;
Morpholines
;
Muscle Contraction
;
Muscle, Smooth
;
Muscles
;
Ondansetron
;
Receptors, Serotonin
;
Receptors, Serotonin, 5-HT1
;
Receptors, Serotonin, 5-HT3
;
Receptors, Serotonin, 5-HT4
;
Relaxation
;
Serotonin
;
Serotonin Receptor Agonists
;
Sulfonamides
;
Tetrodotoxin
4.Mechanism of Interdigestive Migrating Motor Complex.
Journal of Neurogastroenterology and Motility 2012;18(3):246-257
Migrating motor complex (MMC) is well characterized by the appearance of gastrointestinal contractions in the interdigestive state. This review article discussed the mechanism of gastrointestinal MMC. Luminal administration of 5-hydroxytryptamine (5-HT) initiates duodenal phase II followed by gastrointestinal phase III with a concomitant increase of plasma motilin release in conscious dogs. Duodenal 5-HT concentration is increased during gastric phase II and phase III. Intravenous infusion of motilin increases luminal 5-HT content and induces gastrointestinal phase III. 5-HT4 antagonists significantly inhibits both of gastric and intestinal phase III, while 5-HT3 antagonists inhibited only gastric phase III. These suggest that gastrointestinal MMC cycle is mediated via the interaction between motilin and 5-HT by the positive feedback mechanism. Gastric MMC is regulated via vagus, 5-HT3/4 receptors and motilin, while intestinal MMC is regulated via intrinsic primary afferent neurons and 5-HT4 receptors. Stress is highly associated with the pathogenesis of functional dyspepsia. Acoustic stress attenuates gastric phase III without affecting intestinal phase III in conscious dogs, via reduced vagal activity and increased sympathetic activity. It has been shown that subset of functional dyspepsia patients show reduced vagal activity and impaired gastric phase III. The physiological importance of gastric MMC is a mechanical and chemical cleansing of the empty stomach in preparation for the next meal. The impaired gastric MMC may aggravate dyspeptic symptoms following a food ingestion. Thus, maintaining gastric MMC in the interdigestive state is an important factor to prevent the postprandial dyspeptic symptoms.
Acoustics
;
Animals
;
Autonomic Pathways
;
Contracts
;
Dogs
;
Dyspepsia
;
Eating
;
Enterochromaffin Cells
;
Humans
;
Infusions, Intravenous
;
Meals
;
Motilin
;
Myoelectric Complex, Migrating
;
Neurons, Afferent
;
Phenobarbital
;
Plasma
;
Receptors, Serotonin, 5-HT4
;
Serotonin
;
Serotonin 5-HT3 Receptor Antagonists
;
Serotonin 5-HT4 Receptor Antagonists
;
Stomach
5.Strategy to Manage Irritable Bowel Syndrome in Korea.
Jeong Hwan KIM ; In Kyung SUNG
Korean Journal of Medicine 2014;86(6):695-697
Irritable bowel syndrome (IBS) is one of the most prevalent functional gastrointestinal disorders. It is a multi-factorial disorder due to abnormal gastrointestinal motility, low-grade inflammation, visceral hypersensitivity, and communication between the gut-brain axis. IBS is traditionally treated with dietary and lifestyle modifications, fiber supplementation, and psychological and pharmacological therapies. Diet therapy including the low FODMAP diet and excluding certain food constituents is often used. Antispasmodics plus stool consistency modifiers to treat the major symptoms and defecation are first-line drug treatments. 5-Hydroxytryptamine (5-HT) receptors in the gastrointestinal tract, particularly 5-HT3 and 5-HT4 receptors are involved not only in modulating gut motility but in visceral sensory pathways. Drugs that act on both receptor classes appear to reduce visceral sensitivity and have inhibitory effects on motor activity in the distal intestine. 5-HT4 agonists may improve constipation-predominant IBS by normalizing bowel habits and thereby reduce abdominal pain. IBS continues to be a therapeutic challenge because of its diverse symptomatology and lack of a single pathophysiological target for drug intervention.
Abdominal Pain
;
Axis, Cervical Vertebra
;
Defecation
;
Diet
;
Diet Therapy
;
Gastrointestinal Diseases
;
Gastrointestinal Motility
;
Gastrointestinal Tract
;
Hypersensitivity
;
Inflammation
;
Intestines
;
Irritable Bowel Syndrome*
;
Korea
;
Life Style
;
Motor Activity
;
Parasympatholytics
;
Receptors, Serotonin, 5-HT4
;
Serotonin
;
Serotonin 5-HT4 Receptor Agonists
6.Distribution of 5-HT3, 5-HT4, and 5-HT7 Receptors Along the Human Colon.
Nor S YAAKOB ; Kenneth A CHINKWO ; Navinisha CHETTY ; Ian M COUPAR ; Helen R IRVING
Journal of Neurogastroenterology and Motility 2015;21(3):361-369
BACKGROUND/AIMS: Several disorders of the gastrointestinal tract are associated with abnormal serotonin (5-HT) signaling or metabolism where the 5-HT3 and 5-HT4 receptors are clinically relevant. The aim was to examine the distribution of 5-HT3, 5-HT4, and 5-HT7 receptors in the normal human colon and how this is associated with receptor interacting chaperone 3, G protein coupled receptor kinases, and protein LIN-7 homologs to extend previous observations limited to the sigmoid colon or the upper intestine. METHODS: Samples from ascending, transverse, descending, and sigmoid human colon were dissected into 3 separate layers (mucosa, longitudinal, and circular muscles) and ileum samples were dissected into mucosa and muscle layers (n = 20). Complementary DNA was synthesized by reverse transcription from extracted RNA and expression was determined by quantitative or end point polymerase chain reaction. RESULTS: The 5-HT3 receptor subunits were found in all tissues throughout the colon and ileum. The A subunit was detected in all samples and the C subunit was expressed at similar levels while the B subunit was expressed at lower levels and less frequently. The 5-HT3 receptor E subunit was mainly found in the mucosa layers. All splice variants of the 5-HT4 and 5-HT7 receptors were expressed throughout the colon although the 5-HT4 receptor d, g, and i variants were expressed less often. CONCLUSIONS: The major differences in 5-HT receptor distribution within the human colon are in relation to the mucosa and muscular tissue layers where the 5-HT3 receptor E subunit is predominantly found in the mucosal layer which may be of therapeutic relevance.
Colon*
;
Colon, Sigmoid
;
DNA, Complementary
;
G-Protein-Coupled Receptor Kinases
;
Gastrointestinal Tract
;
Humans
;
Ileum
;
Intestines
;
Metabolism
;
Mucous Membrane
;
Polymerase Chain Reaction
;
Receptors, Serotonin
;
Receptors, Serotonin, 5-HT3
;
Receptors, Serotonin, 5-HT4
;
Reverse Transcription
;
RNA
;
Serotonin
7.Serotonin (5-HT) Receptor Subtypes Mediate Regulation of Neuromodulin Secretion in Rat Hypothalamic Neurons.
Genomics & Informatics 2007;5(2):77-82
Serotonin (5-HT), the endogenous nonselective 5-HT receptor agonist, activates the inositol -1,4,5- triphosphate / calcium (InsP3/Ca2+) signaling pathway and exerts both stimulatory and inhibitory actions on cAMP production and neuromodulin secretion in rat hypothalamic neurons. Specific mRNA transcripts for 5-HT1A, 5-HT2C and 5-HT4 were identified in rat hypothalamic neurons. These experiments were supported by combined techniques such as cAMP and a Ca2+ assays in order to elucidate the associated receptors and signaling pathways. The cAMP production and neuromodulin release were profoundly inhibited during the activation of the Gi-coupled 5-HT1A receptor. Treatment with a selective agonist to activate the Gq-coupled 5-HT2C receptor stimulated InsP3 production and caused Ca2+ release from the sarcoplasmic reticulum. Selective activation of the Gs-coupled 5-HT4 receptor also stimulated cAMP production, and caused an increase in neuromodulin secretion. These findings demonstrate the ability of 5-HT receptor subtypes expressed in neurons to induce neuromodulin production. This leads to the activation of single or multiple G-proteins which regulate the InsP3/Ca2+/PLC-gamma and adenyl cyclase / cAMP signaling pathways.
Animals
;
Calcium
;
GAP-43 Protein*
;
GTP-Binding Proteins
;
Inositol
;
Neurons*
;
Rats*
;
Receptor, Serotonin, 5-HT1A
;
Receptor, Serotonin, 5-HT2C
;
Receptors, Serotonin, 5-HT4
;
RNA, Messenger
;
Sarcoplasmic Reticulum
;
Serotonin*
;
Adenylyl Cyclases
8.5-Hydroxytryptamine Generates Tonic Inward Currents on Pacemaker Activity of Interstitial Cells of Cajal from Mouse Small Intestine.
Pawan Kumar SHAHI ; Seok CHOI ; Dong Chuan ZUO ; Cheol Ho YEUM ; Pyung Jin YOON ; Jun LEE ; Young Dae KIM ; Chan Guk PARK ; Man Yoo KIM ; Hye Rang SHIN ; Hyun Jung OH ; Jae Yeoul JUN
The Korean Journal of Physiology and Pharmacology 2011;15(3):129-135
In this study we determined whether or not 5-hydroxytryptamine (5-HT) has an effect on the pacemaker activities of interstitial cells of Cajal (ICC) from the mouse small intestine. The actions of 5-HT on pacemaker activities were investigated using a whole-cell patch-clamp technique, intracellular Ca2+ ([Ca2+]i) analysis, and RT-PCR in ICC. Exogenously-treated 5-HT showed tonic inward currents on pacemaker currents in ICC under the voltage-clamp mode in a dose-dependent manner. Based on RT-PCR results, we found the existence of 5-HT2B, 3, 4, and 7 receptors in ICC. However, SDZ 205557 (a 5-HT4 receptor antagonist), SB 269970 (a 5-HT7 receptor antagonist), 3-tropanylindole - 3 - carboxylate methiodide (3-TCM; a 5-HT3 antagonist) blocked the 5-HT-induced action on pacemaker activity, but not SB 204741 (a 5-HT2B receptor antagonist). Based on [Ca2+]i analysis, we found that 5-HT increased the intensity of [Ca2+]i. The treatment of PD 98059 or JNK II inhibitor blocked the 5-HT-induced action on pacemaker activity of ICC, but not SB 203580. In summary, these results suggest that 5-HT can modulate pacemaker activity through 5-HT3, 4, and 7 receptors via [Ca2+]i mobilization and regulation of mitogen-activated protein kinases.
Animals
;
Flavonoids
;
Gastrointestinal Motility
;
Imidazoles
;
Interstitial Cells of Cajal
;
Intestine, Small
;
Mice
;
Mitogen-Activated Protein Kinases
;
para-Aminobenzoates
;
Patch-Clamp Techniques
;
Phenols
;
Pyridines
;
Receptor, Serotonin, 5-HT2B
;
Receptors, Serotonin
;
Receptors, Serotonin, 5-HT4
;
Serotonin
;
Sulfonamides
9.TREK1 potassium channels and depression.
Dong-Qing YE ; Zhi-Jun ZHANG ; Yang LI
Acta Pharmaceutica Sinica 2012;47(11):1403-1408
Major depression disorder is an increasing heavy burden in modem society, but its pathological mechanism is still vague. Recent evidence indicated that two pore potassium channel, TREK1, is one of the important drug targets of antidepressants. The structural and functional research progress of TREK1 potassium channel were reviewed with an emphasis on its roles in anti-depression, neuronal protection, and neuronal plasticity. The complicated interactions between TREK1 potassium channel and monoamine transmitters-receptors were also reviewed and future directions to explore the underline mechanism were also discussed.
Animals
;
Antidepressive Agents
;
pharmacology
;
Depressive Disorder, Major
;
genetics
;
metabolism
;
physiopathology
;
Drug Delivery Systems
;
Gene Knockout Techniques
;
Humans
;
Neuronal Plasticity
;
Polymorphism, Genetic
;
Potassium Channels, Tandem Pore Domain
;
genetics
;
metabolism
;
physiology
;
Receptors, Serotonin
;
metabolism
;
Receptors, Serotonin, 5-HT4
;
Serotonin
;
pharmacology
10.Treatment of Constipation.
Korean Journal of Medicine 2011;80(5):510-523
Constipation is a common gastrointestinal disease affecting approximately 16.5% of the population in Korea. Systemic diseases such as hypothyroidism or colon cancer and drugs can cause constipation in some patients with constipation, there is no obstructive mucosal or structural cause in the vast majority of patients with constipation. Evaluation for secondary causes of constipation is needed to provide appropriate management. Once secondary causes have been excluded, constipation may be classified into normal or slow transit constipation, evacuation disorder of the spastic or flaccid varieties, or both. Treatment of chronic constipation based on the underlying pathophysiology is generally successful. The aims of this review are to discuss the management of functional constipation based on guidelines for the treatment of constipation published in Korean Journal of Gastroenterology in 2011: lifestyle changes; bulking agents and stool softeners; osmotic agents; stimulant laxatives; prokinetics; biofeedback and surgical treatments. Exercise and dietary fiber are helpful in some patients with constipation. Laxatives including bulking agents, stool softeners, osmotic agents, stimulant laxatives have been found to be more effective than placebo at relieving symptoms of constipation. New enterokinetic agents such as 5-hydroxytryptamine-4 receptor agonists, intestinal secretagogues, and peripheral opioid antagonists could be effective in patients with constipation who cannot get adequate relief from current laxatives. Biofeedback could relieve symptoms in selected patients with constipation due to pelvic floor dyssynergia. Surgical treatments can be helpful in some patients with refractory constipation.
Ataxia
;
Biofeedback, Psychology
;
Colonic Neoplasms
;
Constipation
;
Dietary Fiber
;
Gastroenterology
;
Gastrointestinal Diseases
;
Humans
;
Hypothyroidism
;
Korea
;
Laxatives
;
Life Style
;
Muscle Spasticity
;
Narcotic Antagonists
;
Pelvic Floor
;
Receptors, Serotonin, 5-HT4