3.Action Mechanisms of Hormone Binding to Cell Surface Receptors: 1) G-protein Coupled Receptor Signalling as Targets for Hormone Action: (2) The Role and the Regulation of Phospolipase C.
Journal of Korean Society of Endocrinology 2000;15(3):333-341
No Abstract Available.
GTP-Binding Proteins*
;
Receptors, Cell Surface*
4.Action Mechanisms of Hormone Binding to Cell Surface Receptors: 1) G-protein Coupled Receptor Signalling as Targets for Hormone Action: (1) Adenylate Cyclase Cascade.
Journal of Korean Society of Endocrinology 2000;15(3):311-332
No Abstract Available.
Adenylyl Cyclases*
;
GTP-Binding Proteins*
;
Receptors, Cell Surface*
5.A new mutation of PTCH gene in a Chinese family with nevoid basal cell carcinoma syndrome.
Yan LÜ ; Han-guang ZHU ; Wei-min YE ; Ming-bin ZHANG ; Di HE ; Wan-tao CHEN
Chinese Medical Journal 2008;121(2):118-121
BACKGROUNDNevoid basal cell carcinoma syndrome (NBCCS) is a rare autosomal dominant disease characterized by a combination of development anomalies and a predisposition to tumour formation. Mutation of patched gene (PTCH), considered the molecular defect of NBCCS, in a Chinese NBCCS family was investigated in this study.
METHODSGenomic DNA was isolated from blood samples of all 12 members of this family. The mutated PTCH gene was screened by polymerase chain reaction amplification and direct sequencing.
RESULTSA new mutation of 3 bp (GAT deletion) was found in all seven affected members of this family. This mutation caused one aspartate deletion in the fourth transmembrane domain of the PTCH protein located within the sterol sensing domain (SSD). This deletion was not found in any unaffected members of this family nor in 200 control samples.
CONCLUSIONSOur findings suggest that one 3-bp deletion in PTCH gene was the cause of nevoid basal cell carcinoma in a Chinese family through affecting the conformation and function of PTCH protein.
Basal Cell Nevus Syndrome ; genetics ; Humans ; Mutation ; Patched Receptors ; Patched-1 Receptor ; Receptors, Cell Surface ; genetics
6.Toll-like receptor and innate immunity in phylogenesis.
Ye LIU ; Li-ping ZHU ; Wei HE
Acta Academiae Medicinae Sinicae 2002;24(4):433-436
In protection against microbes, an organism recognizes the pathogen associated molecular pattern (PAMP) on microbes by pattern recognition receptor (PRR). Toll-like receptor is called innate immunity. A family of cell membrane receptor was found in recent years that can mediate innate immune responses through the activation of a series of immune-related genes. In phylogenesis, it is highly conservative. However, its functions are getting more diversified with the complication of the immune functions of organisms.
Animals
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Humans
;
Immunity
;
Membrane Glycoproteins
;
immunology
;
Phylogeny
;
Receptors, Cell Surface
;
immunology
;
Toll-Like Receptors
7.Sepsis and membrane receptors.
Zhao-xia DUAN ; Pei-fang ZHU ; Jian-xin JIANG
Chinese Journal of Traumatology 2005;8(1):60-64
9.Renal amyloidosis and hepatitis B virus-associated glomerulonephritis in a patient with nephrotic syndrome.
Wei JIANG ; Yan XU ; Guangju GUAN ; Ruixia MA ; Hui DONG
Chinese Medical Journal 2014;127(6):1199-1199
Biomarkers
;
blood
;
Female
;
Humans
;
Interleukins
;
blood
;
Male
;
Myocardial Infarction
;
blood
;
Receptors, Cell Surface
;
blood
10.Expression of renin/prorenin receptor in rat kidney and cultured mesangial cells.
Ming HE ; Ya-Lin HUANG ; Lin ZHANG ; Tai YAO ; Li-Min LU
Acta Physiologica Sinica 2007;59(6):796-804
The renin/prorenin receptor (RnR) has recently been cloned and demonstrated to exist in different cells in the cardiovascular and renal systems, playing an important role in physiological and pathophysiological situations. In the present study, we used immunofluorescence method to identify whether and where the RnR expressed in cultured rat renal mesangial cells (MCs) and rat kidney. By using the prorenin handle region peptide (HRP) as a decoy peptide of the RnR, we observed the distribution of the HRP-RnR complex in the MCs. Our results showed that the RnR was localized in the perinuclear zone and plasma membrane of the MCs. At the organ level, the RnR was observed in the mesangium of cortical glomeruli in rat kidney. The FITC-labeled HRP (FITC-HRP) translocated from cell culture medium into the cytoplasm within 30 s. Colocalization of the HRP and RnR was observed mainly on the cell membrane and in the perinuclear zone of cytoplasm by using immunofluorescence and confocal microscopy. At 30 min the FITC-HRP was mainly observed in the nucleus while the RnR remained in the perinuclear zone of cytoplasm. Taken together, our results confirm the expression of RnR in the renal MCs. It is suggested that internalization of the RnR after binding with its ligand is at least one of the pathways through which the RnR exerts its biological actions.
Animals
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Kidney
;
metabolism
;
Mesangial Cells
;
metabolism
;
Rats
;
Receptors, Cell Surface
;
metabolism
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