1.Chemokine axes CXCL12/CXCR4 and CXCL16/CXCR6 correlate with lymph node metastasis in epithelial ovarian carcinoma.
Li GUO ; Zhu-Mei CUI ; Jia ZHANG ; Yu HUANG
Chinese Journal of Cancer 2011;30(5):336-343
Recent evidence suggests that the chemokine axis of CXC chemokine ligand-12 and its receptor CXC chemokine receptor-4 (CXCL12/CXCR4) is highly expressed in gynecological tumors and the axis of CXC chemokine ligand-16 and CXC chemokine receptor-6 (CXCL16/CXCR6) is overexpressed in inflammation-associated tumors. This study aimed to determine the relationship between CXCL12/CXCR4, CXCL16/CXCR6 and ovarian carcinoma's clinicopathologic features and prognosis. Accordingly, the expression of these proteins in ovarian tissues was detected by tissue microarray and immunohistochemistry. The expressions of CXCL12/CXCR4 and CXCL16/CXCR6 were significantly higher in epithelial ovarian carcinomas than in normal epithelial ovarian tissues or benign epithelial ovarian tumors. The expression of chemokines CXCL12 and CXCL16 were positively correlated with their receptors CXCR4 and CXCR6 in ovarian carcinoma, respectively (r = 0.300, P < 0.05; r = 0.395, P < 0.05). Moreover, the expression of CXCL12 was related to the occurrence of ascites (Χ² = 4.76, P < 0.05), the expression of CXCR4 was significantly related to lymph node metastasis (Χ(2) = 4.37, P < 0.05), the expression of CXCR6 was significantly related to lymph node metastasis (Χ² = 7.43, P < 0.05) and histological type (Χ² = 33.48, P < 0.05). In univariate analysis, the expression of CXCR4 and CXCL16 significantly correlated with reduced median survival (Χ² = 4.67, P < 0.05; Χ² = 4.48, P < 0.05). Therefore, we conclude that the chemokine axes CXCL12/CXCR4 and CXCL16/CXCR6 may play important roles in the growth, proliferation, invasion, and metastasis of epithelial ovarian carcinoma.
Adolescent
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Adult
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Aged
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Ascites
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pathology
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Chemokine CXCL12
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metabolism
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Chemokine CXCL16
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Chemokines, CXC
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metabolism
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Cystadenocarcinoma, Mucinous
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metabolism
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pathology
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Cystadenocarcinoma, Serous
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metabolism
;
pathology
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Female
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Follow-Up Studies
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Humans
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Lymphatic Metastasis
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Middle Aged
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Ovarian Neoplasms
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metabolism
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pathology
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Receptors, CXCR4
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metabolism
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Receptors, CXCR6
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Receptors, Chemokine
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metabolism
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Receptors, Scavenger
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metabolism
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Receptors, Virus
;
metabolism
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Survival Rate
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Young Adult
2.Expression of the CXCL12/CXCR4 and CXCL16/CXCR6 axes in cervical intraepithelial neoplasia and cervical cancer.
Yu HUANG ; Jia ZHANG ; Zhu-Mei CUI ; Jing ZHAO ; Ye ZHENG
Chinese Journal of Cancer 2013;32(5):289-296
The chemokine CXCL12 is highly expressed in gynecologic tumors and is widely known to play a biologically relevant role in tumor growth and spread. Recent evidence suggests that CXCL16, a novel chemokine, is overexpressed in inflammation-associated tumors and mediates pro-tumorigenic effects of inflammation in prostate cancer. We therefore analyzed the expression of CXCL12 and CXCL16 and their respective receptors CXCR4 and CXCR6 in cervical intraepithelial neoplasia (CIN) and cervical cancer and further assessed their association with clinicopathologic features and outcomes. Tissue chip technology and immunohistochemistry were used to analyze the expression of CXCL12, CXCR4, CXCL16, and CXCR6 in healthy cervical tissue (21 cases), CIN (65 cases), and cervical carcinoma (60 cases). The association of protein expression with clinicopathologic features and overall survival was analyzed. These four proteins were clearly detected in membrane and cytoplasm of neoplastic epithelial cells, and their distribution and intensity of expression increased as neoplastic lesions progressed through CIN1, CIN2, and CIN3 to invasive cancer. Furthermore, the expression of CXCR4 was associated significantly with the histologic grade of cervical carcinoma, whereas the expression of CXCR6 was associated significantly with lymph node metastasis. In Kaplan-Meier analysis, patients with high CXCR6 expression had significantly shorter overall survival than did those with low CXCR6 expression. The elevated co-expression levels of CXCL12/CXCR4 and CXCL16/CXCR6 in CIN and cervical carcinoma suggest a durative process in cervical carcinoma development. Moreover, CXCR6 may be useful as a biomarker and a valuable prognostic factor for cervical cancer.
Adult
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Aged
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Biomarkers, Tumor
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metabolism
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Carcinoma, Squamous Cell
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metabolism
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pathology
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Cervical Intraepithelial Neoplasia
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metabolism
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pathology
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Chemokine CXCL12
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metabolism
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Chemokine CXCL16
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Chemokines, CXC
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metabolism
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Epithelial Cells
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metabolism
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Female
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Follow-Up Studies
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Humans
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Lymphatic Metastasis
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Middle Aged
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Neoplasm Grading
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Neoplasm Staging
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Receptors, CXCR4
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metabolism
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Receptors, CXCR6
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Receptors, Chemokine
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metabolism
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Receptors, Scavenger
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metabolism
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Receptors, Virus
;
metabolism
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Survival Rate
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Uterine Cervical Neoplasms
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metabolism
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pathology