1.Expressions of MIP-1alpha, MCP-1 and their receptors CCR-1, CCR-2 in chronic myeloid leukemia cells.
Wei-Liang WANG ; Ti SHEN ; Yu-Rong HUI ; Xi-Chun GU ; Rong-Sheng LI
Journal of Experimental Hematology 2006;14(3):433-436
This study was aimed to explore the expression of MIP-1alpha, MCP-1 and their receptors CCR-1, CCR-2 in bcr/abl fusion gene positive CML cells, and to study the effects of P210(bcr/abl) fusion protein tyrosine kinase on expression of MIP-1alpha, MCP-1 and their receptors CCR-1, CCR-2 mRNAs in chronic myeloid leukemia cells. The expression levels of MIP-1alpha, MCP-1 and their receptors CCR-1, CCR-2 mRNA were detected by semi-quantitative RT-PCR in bcr/abl negative cells, bcr/abl positive cells, and P210(bcr/abl)-Rb-C-Box positive cells. The results showed that MIP-1alpha and CCR-1 mRNAs were expressed in bcr/abl negative cells, but not in positive cells. Both MCP-1 and CCR-2 mRNA cannot be detected in both bcr/abl positive and negative cells. After inhibiting P210(bcr/abl) tyrosine kinase activity by Rb-C-Box, expressions of MIP-1alpha and CCR-1 mRNAs were restored to normal (similar to P210(bcr/abl) negative cells). It is concluded that P210(bcr/abl) fusion protein inhibits the expression of MIP-1alpha and CCR-1 in chronic myeloid leukemia cells, but does not inhibit MCP-1 and CCR-2 mRNA expressions in these leukemia cells.
Chemokine CCL2
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biosynthesis
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genetics
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Chemokine CCL3
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Chemokine CCL4
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Humans
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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metabolism
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Macrophage Inflammatory Proteins
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biosynthesis
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genetics
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Receptors, CCR1
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Receptors, CCR2
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Receptors, Chemokine
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biosynthesis
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genetics
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Tumor Cells, Cultured
2.Human LZIP induces monocyte CC chemokine receptor 2 expression leading to enhancement of monocyte chemoattractant protein 1/CCL2-induced cell migration.
Ho Joong SUNG ; Yoon Suk KIM ; Hyereen KANG ; Jesang KO
Experimental & Molecular Medicine 2008;40(3):332-338
Chemokines and chemokine receptors play a role in migration of circulating leukocytes to the region of inflammation. Human LZIP is an uncharacterized transcription factor and is known to participate in leukotactin (Lkn)-1/CCL15-induced cell migration. We investigated the role of human LZIP in expression of CC chemokine receptors (CCRs) and its involvement in monocyte migration. RNase protection analysis showed that LZIP increased mRNA expression of CCR2 and CCR1 in THP-1 cells. Surface expressions of both CCR2 and CCR1 were also increased by LZIP. Results from an electrophoretic mobility shift assay showed that LZIP binds to the C/EBP element in the CCR2 promoter. LZIP also enhanced the chemotactic activities of monocyte chemoattractant protein-1/CCL2 and Lkn-1. These results suggest that LZIP regulates expression of chemokine receptors that are involved in monocyte migration.
Atherosclerosis/drug therapy/etiology
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CCAAT-Enhancer-Binding Proteins/genetics/immunology/*metabolism
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Cell Line, Tumor
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Cell Movement/drug effects/*physiology
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Chemokine CCL2/*pharmacology
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Chemokines, CC/pharmacology
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Cyclic AMP Response Element-Binding
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Humans
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Macrophage Inflammatory Proteins/pharmacology
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Monocytes/drug effects/metabolism
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Promoter Regions, Genetic
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Protein Binding
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RNA, Messenger/analysis
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Receptors, CCR1/biosynthesis/genetics
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Receptors, CCR2/*biosynthesis/genetics
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Transcriptional Activation/drug effects
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Transfection
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Transgenes