1.The role of ferroptosis in chronic diseases.
Junyi CHEN ; Xiang YANG ; Xuexian FANG ; Fudi WANG ; Junxia MIN
Journal of Zhejiang University. Medical sciences 2020;49(1):44-57
Recently, ferroptosis, an iron-dependent novel type of cell death, has been characterized as an excessive accumulation of lipid peroxides and reactive oxygen species. Emerging studies demonstrate that ferroptosis not only plays an important role in the pathogenesis and progression of chronic diseases, but also functions differently in the different disease context. Notably, it is shown that activation of ferroptosis could potently inhibit tumor growth and increase sensitivity to chemotherapy and immunotherapy in various cancer settings. As a result, the development of more efficacious ferroptosis agonists remains the mainstay of ferroptosis-targeting strategy for cancer therapeutics. By contrast, in non-cancerous chronic diseases, including cardiovascular & cerebrovascular diseases and neurodegenerative diseases, ferroptosis functions as a risk factor to promote these diseases progression through triggering or accelerating tissue injury. As a matter of fact, blocking ferroptosis has been demonstrated to effectively prevent ischemia-reperfusion heart disease in preclinical animal models. Therefore, it is a promising field to develope potent ferroptosis inhibitors for preventing and treating cardiovascular & cerebrovascular diseases and neurodegenerative diseases. In this article, we summarize the most recent progress on ferroptosis in chronic diseases, and draw attention to the possible clinical impact of this recently emerged ferroptosis modalities.
Animals
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Chronic Disease
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Ferroptosis
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physiology
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Iron
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metabolism
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Reactive Oxygen Species
2.Attenuation of Oxidative Stress-Induced Cell Apoptosis and Pyroptosis in RSC96 Cells by Salvianolic Acid B.
Qian-Qian WANG ; Meng WANG ; Yan LI ; Yong-Hui LIU ; Lian-Qing SUN
Chinese journal of integrative medicine 2022;28(3):243-248
OBJECTIVE:
To determine whether salvianolic acid B (Sal B) exerts protective effects on diabetic peripheral neuropathy by attenuating apoptosis and pyroptosis.
METHODS:
RSC96 cells were primarily cultured with DMEM (5.6 mmol/L glucose), hyperglycemia (HG, 125 mmol/L glucose) and Sal B (0.1, 1, and 10 µ mol/L). Cells proliferation was measured by 3-(4, 5-cimethylthiazol-2-yl)-2, 5-dilphenyltetrazolium bromide assay. Reactive oxygen species (ROS) generation and apoptosis rate were detected by flow cytometry analysis. Western blot was performed to analyze the expressions of poly ADP-ribose polymerase (PARP), cleaved-caspase 3, cleaved-caspase 9, Bcl-2, Bax, NLRP3, ASC, and interleukin (IL)-1β.
RESULTS:
Treatment with HG at a concentration of 125 mmol/L attenuated cellular proliferation, while Sal B alleviated this injury (P<0.05). In addition, Sal B inhibited HG-induced ROS production and apoptosis rate (P<0.05). Furthermore, treatment with Sal B down-regulated HG-induced PARP, cleaved-caspase 3, cleaved-caspase 9, Bax, NLRP3, ASC, and IL-1β expression, but mitigated HG-mediated down-regulation of Bcl-2 expression (P<0.05).
CONCLUSION
Sal B may protect RSC96 cells against HG-induced cellular injury via the inhibition of apoptosis and pyroptosis activated by ROS.
Apoptosis
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Benzofurans/pharmacology*
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Oxidative Stress
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Pyroptosis
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Reactive Oxygen Species/metabolism*
4.Regulation of Stem Cell Fate by ROS-mediated Alteration of Metabolism.
Jung Min RYU ; Hyun Jik LEE ; Young Hyun JUNG ; Ki Hoon LEE ; Dah Ihm KIM ; Jeong Yeon KIM ; So Hee KO ; Gee Euhn CHOI ; Ing Ing CHAI ; Eun Ju SONG ; Ji Young OH ; Sei Jung LEE ; Ho Jae HAN
International Journal of Stem Cells 2015;8(1):24-35
Stem cells have attracted much attention due to their distinct features that support infinite self-renewal and differentiation into the cellular derivatives of three lineages. Recent studies have suggested that many stem cells both embryonic and adult stem cells reside in a specialized niche defined by hypoxic condition. In this respect, distinguishing functional differences arising from the oxygen concentration is important in understanding the nature of stem cells and in controlling stem cell fate for therapeutic purposes. ROS act as cellular signaling molecules involved in the propagation of signaling and the translation of environmental cues into cellular responses to maintain cellular homeostasis, which is mediated by the coordination of various cellular processes, and to adapt cellular activity to available bioenergetic sources. Thus, in this review, we describe the physiological role of ROS in stem cell fate and its effect on the metabolic regulation of stem cells.
Adult Stem Cells
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Cues
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Energy Metabolism
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Glucose
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Homeostasis
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Metabolism*
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Oxygen
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Reactive Oxygen Species
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Stem Cells*
5.The excessive response: a preparation for harder conditions.
Protein & Cell 2017;8(10):707-710
6.Research progress on the role of chondrocyte mitochondrial homeostasis imbalance in the pathogenesis of osteoarthritis.
Quan CHEN ; Limin WU ; Cili DAWA ; Bin SHEN
Chinese Journal of Reparative and Reconstructive Surgery 2023;37(6):748-757
OBJECTIVE:
To summarize the role of chondrocyte mitochondrial homeostasis imbalance in the pathogenesis of osteoarthritis (OA) and analyze its application prospects.
METHODS:
The recent literature at home and abroad was reviewed to summarize the mechanism of mitochondrial homeostasis imbalance, the relationship between mitochondrial homeostasis imbalance and the pathogenesis of OA, and the application prospect in the treatment of OA.
RESULTS:
Recent studies have shown that mitochondrial homeostasis imbalance, which is caused by abnormal mitochondrial biogenesis, the imbalance of mitochondrial redox, the imbalance of mitochondrial dynamics, and damaged mitochondrial autophagy of chondrocytes, plays an important role in the pathogenesis of OA. Abnormal mitochondrial biogenesis can accelerate the catabolic reaction of OA chondrocytes and aggravate cartilage damage. The imbalance of mitochondrial redox can lead to the accumulation of reactive oxygen species (ROS), inhibit the synthesis of extracellular matrix, induce ferroptosis and eventually leads to cartilage degradation. The imbalance of mitochondrial dynamics can lead to mitochondrial DNA mutation, decreased adenosine triphosphate production, ROS accumulation, and accelerated apoptosis of chondrocytes. When mitochondrial autophagy is damaged, dysfunctional mitochondria cannot be cleared in time, leading to ROS accumulation, which leads to chondrocyte apoptosis. It has been found that substances such as puerarin, safflower yellow, and astaxanthin can inhibit the development of OA by regulating mitochondrial homeostasis, which proves the potential to be used in the treatment of OA.
CONCLUSION
The mitochondrial homeostasis imbalance in chondrocytes is one of the most important pathogeneses of OA, and further exploration of the mechanisms of mitochondrial homeostasis imbalance is of great significance for the prevention and treatment of OA.
Humans
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Reactive Oxygen Species/metabolism*
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Chondrocytes/metabolism*
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Osteoarthritis/metabolism*
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Homeostasis
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Mitochondria/metabolism*
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Cartilage, Articular/metabolism*
7.Molecular Mechanisms of Ventilator-Induced Lung Injury.
Lin CHEN ; Hai-Fa XIA ; You SHANG ; Shang-Long YAO
Chinese Medical Journal 2018;131(10):1225-1231
ObjectiveMechanical ventilation (MV) has long been used as a life-sustaining approach for several decades. However, researchers realized that MV not only brings benefits to patients but also cause lung injury if used improperly, which is termed as ventilator-induced lung injury (VILI). This review aimed to discuss the pathogenesis of VILI and the underlying molecular mechanisms.
Data SourcesThis review was based on articles in the PubMed database up to December 2017 using the following keywords: "ventilator-induced lung injury", "pathogenesis", "mechanism", and "biotrauma".
Study SelectionOriginal articles and reviews pertaining to mechanisms of VILI were included and reviewed.
ResultsThe pathogenesis of VILI was defined gradually, from traditional pathological mechanisms (barotrauma, volutrauma, and atelectrauma) to biotrauma. High airway pressure and transpulmonary pressure or cyclic opening and collapse of alveoli were thought to be the mechanisms of barotraumas, volutrauma, and atelectrauma. In the past two decades, accumulating evidence have addressed the importance of biotrauma during VILI, the molecular mechanism underlying biotrauma included but not limited to proinflammatory cytokines release, reactive oxygen species production, complement activation as well as mechanotransduction.
ConclusionsBarotrauma, volutrauma, atelectrauma, and biotrauma contribute to VILI, and the molecular mechanisms are being clarified gradually. More studies are warranted to figure out how to minimize lung injury induced by MV.
Animals ; Barotrauma ; metabolism ; Humans ; Reactive Oxygen Species ; metabolism ; Ventilator-Induced Lung Injury ; metabolism ; Wounds and Injuries ; metabolism
8.Research of different effects on activity of plant antioxidant enzymes.
Xuan LI ; Hong YUE ; Sheng WANG ; Lu-Qi HUANG ; Jiong MA ; Lan-Ping GUO
China Journal of Chinese Materia Medica 2013;38(7):973-978
Plant can produce reactive oxygen species (ROS) in normal physiological activity. However, ROS can increase dramatically by environmental stress. The increase may break the ROS balance in the plant. To control ROS levels in plants, an antioxidant system is needed to protect the cell. This paper introduced the types and functions of antioxidant enzymes, and studied the effects of plant and environmental factors on the activities of antioxidant enzymes. At last, the paper discussed the research hot fields of the effect of environmental stress on antioxidant enzymes, and gave the research strategies to the problems which happened in the studies.
Antioxidants
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metabolism
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Ecosystem
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Oxidative Stress
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Plant Proteins
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metabolism
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Plants
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enzymology
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metabolism
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Reactive Oxygen Species
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metabolism
9.Role of the Voltage-Gated Proton Channel Hv1 in Nervous Systems.
Yu SHEN ; Yuncheng LUO ; Ping LIAO ; Yunxia ZUO ; Ruotian JIANG
Neuroscience Bulletin 2023;39(7):1157-1172
Hv1 is the only voltage-gated proton-selective channel in mammalian cells. It contains a conserved voltage-sensor domain, shared by a large class of voltage-gated ion channels, but lacks a pore domain. Its primary role is to extrude protons from the cytoplasm upon pH reduction and membrane depolarization. The best-known function of Hv1 is the regulation of cytosolic pH and the nicotinamide adenine dinucleotide phosphate oxidase-dependent production of reactive oxygen species. Accumulating evidence indicates that Hv1 is expressed in nervous systems, in addition to immune cells and others. Here, we summarize the molecular properties, distribution, and physiological functions of Hv1 in the peripheral and central nervous systems. We describe the recently discovered functions of Hv1 in various neurological diseases, including brain or spinal cord injury, ischemic stroke, demyelinating diseases, and pain. We also summarize the current advances in the discovery and application of Hv1-targeted small molecules in neurological diseases. Finally, we discuss the current limitations of our understanding of Hv1 and suggest future research directions.
Animals
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Protons
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Ion Channels/metabolism*
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Reactive Oxygen Species/metabolism*
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Brain/metabolism*
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NADPH Oxidases
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Mammals/metabolism*