1. GC-MS analysis and anti-mosquito activities of Juniperus virginiana essential oil against Anopheles stephensi (Diptera: Culicidae)
Azar TAHGHIGHI ; Azar TAHGHIGHI ; Naseh MALEKI-RAVASAN ; Navid DINPARAST DJADID ; Raziyeh AHMADVAND ; Naseh MALEKI-RAVASAN ; Hamzeh ALIPOUR ; Fateh KARIMIAN ; Saeed YOUSEFINEJAD
Asian Pacific Journal of Tropical Biomedicine 2019;9(4):168-175
Objective: To investigate phytochemicals present in the essential oil from aerial parts of eastern red cedar, Juniperus virginiana (J. virginiana) L. (Cupressaceae) and to determine its killing and repellent activities against larvae, pupae, and adults of the Asian malaria mosquito, Anopheles stephensi (Diptera: Culicidae). Methods: J. virginiana essential oil was extracted by hydrodistillation, and its chemical composition was determined by gas chromatography-mass spectrometry. Seven different logarithmic concentrations of J. virginiana essential oils were used in larvicidal and pupicidal assays. J. virginiana essential oils-impregnated bed nets were applied in a designed animal module to test excito-repellent activity against adult mosquitoes. Results: Fourteen constituents corresponding to 99.98% of J. virginiana essential oils were identified. Five main components were terpinen-4-ol (25.21%), camphor (19.89%), E-3-hexen-1-ol (13.30%), γ-terpinene (7.86%), and l-menthone (2.27%). The LC
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