1.The effects of different types of media on in vitro maturation outcomes of human germinal vesicle oocytes retrieved in intracytoplasmic sperm injection cycles.
Farzaneh FESAHAT ; Razieh DEHGHANI FIROUZABADI ; Azita FARAMARZI ; Mohammad Ali KHALILI
Clinical and Experimental Reproductive Medicine 2017;44(2):79-84
OBJECTIVE: Optimizing in vitro maturation (IVM) media to achieve better outcomes has been a matter of interest in recent years. The aim of this prospective clinical trial was to investigate the effects of different media on the IVM outcomes of immature oocytes at the germinal vesicle (GV) stage. METHODS: A total of 400 immature oocytes at the GV stage with normal morphology were retrieved from 320 infertile women aged 31±4.63 years during stimulated intracytoplasmic sperm injection (ICSI) cycles. They were divided into groups of homemade IVM medium (I, n=100), cleavage medium (II, n=100), blastocyst medium (III, n=100), and Sage IVM medium (IV, n=100) and cultured for 24 to 48 hours at 37℃. ICSI was performed, and the rates of fertilization and embryo formation were compared across the four groups. RESULTS: In the 400 retrieved GV oocytes, the total maturation rates showed significant differences in groups I to IV (55%, 53%, 78%, and 68%, respectively, p<0.001). However, there were no significant differences in the fertilization, embryo formation, or arrest rates of metaphase II oocytes across these groups. In all groups, GV maturation was mostly completed after 24 hours, with fewer oocytes requiring 48 hours to mature (p<0.01). Moreover, the rate of high-quality embryos was higher in group IV than in the other groups (p=0.01). CONCLUSION: The quality of the IVM medium was found to affect clinical IVM outcomes. Additionally, blastocyst medium may be a good choice in IVM/ICSI cycles as an alternative IVM medium.
Blastocyst
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Embryonic Structures
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Female
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Fertilization
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Humans*
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In Vitro Techniques*
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Metaphase
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Oocytes*
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Prospective Studies
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Sperm Injections, Intracytoplasmic*
2.Comparison of growth parameters of 5-year-old singleton children born in assisted versus natural conception.
Razieh FALLAH ; Mohammad Taghi JALALIAN ; Motahhareh GOLESTAN ; Razieh DEHGHANI-FIROUZABADI
Annals of the Academy of Medicine, Singapore 2013;42(2):80-84
INTRODUCTIONAt present, about 1% of newborns are delivered through assisted reproductive technologies (ART) worldwide. This study aimed to evaluate and compare the growth parameters of children born in assisted and natural conception at 5 years of age.
MATERIALS AND METHODSIn a cross-sectional case control study, weight, height and head circumference of 5-year old children were assessed. The case group consisted of term, singleton babies who were products of ART in the Center for Infertility of Shahid Sadoughi University, Yazd, Iran in 2005. The control group consisted of term, fi rst child, singleton and spontaneously conceived 5-year-old children who were referred for vaccination to primary health care center of Shahid Akbari in 2010.
RESULTSFifty-eight girls (47.5%) and 64 boys (52.5%) "with equal numbers in each of the 2 groups" were evaluated. Sex distribution, mean ages of fathers and mothers were not statistically significant different in both groups. Children born after ART tend to have lower birth weight, smaller birth head circumference and lower weight at 5 years of age. Having low birth weight (<2500 g), being underweight and having short stature at the age of 5 were more common in babies born through ART.
CONCLUSIONGrowth retardation is more prevalent in babies born through ART. Thus, growth assessment, parents' knowledge about child physical development, and timely and accurate follow-up of these children are necessary for early detection of any growth disorders.
Body Height ; Body Weight ; Child, Preschool ; Cross-Sectional Studies ; Female ; Growth Charts ; Growth Disorders ; diagnosis ; etiology ; Head ; anatomy & histology ; Humans ; Male ; Reproductive Techniques, Assisted ; adverse effects