Objectives: This study aims to identify the relationship between dietary intakes of β-carotene with riskof oral cancer. Methods: A hospital-based, case-control study was conducted on 306 Malaysians whoseek treatment at participating centres/hospitals. Subjects selected from the Malaysian Oral Cancer Dataand Tissue Banking System (MOCDTBS) consisted of 153 cases and 153 controls that were matchedfor gender, age (±5 years) and ethnicity. Food consumption was measured using Food FrequencyQuestionnaire (FFQ). NutrieMart Version 2.0.0 software was used to estimate daily nutrient of eachsubject from the FFQ. Logistic Regression analysis was conducted to compute the odds ratio (OR) forintakes of β-carotene and oral cancer risk. Results: Intake of β-carotene was found to be not associatedwith risk of oral cancer (OR 0.83, 95%CI: 0.42-1.66, p>0.05). Conclusion: No significant associationwas found between dietary intakes of β-carotene with oral cancer risk in this study population.