Objective To align the mapping points from the locations of catheter electrodes with CT/MR image three-dimension data of the cardiac chamber. Methods Most of existing algorithms assumed that two spaces spanned respectively by mapping points and by CT/MR data were orthogonal, and thus modulated only 9 free-degrees of three-dimension translations, rotations and scaling for registration. However, our research showed that the above two spaces might be not always orthogonal. So we introduced affine transformation model based on 12 free-degrees and the corresponding iterative closest point algorithm to realize the registration and further incorporate the registration algorithm with the clinical operations. Combining with OpenGL, we realized the proposed algorithm via Visual C]+]+ 6.0 software. Results Simulation results showed that the residual registration errors on average were decreased from 40 pixels before registration to 1.5 pixels after registration. This algorithm was further validated in animal experiments. Conclusion This proposed three-dimension mapping registration algorithm well realizes the registration in three-dimension cardiac mapping and has practical application values.

Objective To observe the clinical efficacy of therapy of nourishing kidney and liver for the treatment of hypomenorrhea with the syndrome of kidney deficiency and liver stagnation. Methods Fifty-one hypomenorrhea patients with the syndrome of kidney deficiency and liver stagnation were randomized into treatment group(N =25) and control group (N = 26). The treatment group was treated with oral use of Yangyin Shugan Capsules (mainly composed of Radix Bupleuri, Radix Paeoniae Alba, Radix Rehmanniae Preparata, and Fructus Ligustri Lucidi). The control group was given placebo capsules orally. After treatment for 8 weeks , the overall efficiency and therapeutic effect on menstruation of the two groups were evaluated according the changes of traditional Chinese medicine(TCM) symptom scores. Results(1) The total effective rate of the treatment group was 84.0％, and that of the control group was 15.4％, the difference being significant between the two groups (P < 0.01). (2) The effective rate on the menstrual volume and menstrual color and texture of the treatment group was 64.0％, 68.0％, and that of the control group was 7.7％, 15.4％ respectively, the difference being significant(P < 0.01). However, the difference of effect on improving menstrual period was insignificant between the two groups (P > 0.05). Conclusion Therapy of nourishing kidney and liver is effective for the treatment of hypomenorrhea with the syndrome of kidney deficiency and liver stagnation, and has obvious effect on improving menstrual volume and TCM symptoms.

The latest literatures on eslicarbazepine acetate in the treatment of epilepsy from home and abroad were referred. The results showed that the oral absorption of eslicarbazepine acetate is quick with long half life, linear pharmacokinetics and low potential of drug-drug interactions. Compared with carbamazepine and oxcarbazepine, eslicarbazepine acetate is more effective against epilepsy.

Referred to the latest literatures on raltegravir from home and abroad, the effect and safety of raltegravir in the treat-ment of AIDs were reviewed. The results showed that raltegravir was a necessary component for the initial treatment and repeated treat-ment of AIDs. Up till now, there was no report on its serious adverse reactions and drug interactions.

Objective To study the clinical efficacy of treatment of atrophic scars of facial hemangioma after isotopes radiotherapy with micro-plasma.Methods A total of 52 patients with atrophic scars were selected under micro-plasma treatment for 4 times,one time every six weeks.And their efficacies were evaluated by compared with single standard of that before and after the treatment.Results By means of rate of clinical index,the total effective rate of atrophic scars was 82.7％.The degree of improvement was as follows:cured in 53.8％,marked effective in 28.9％,effective in 17.3％ and no case ineffective.The total effective rate of hyperpigmentation was 90.3％,including cured in 59.6％,marked effective in 30.8％,effective in 9.6％ and no ineffective cases.Conclusions Micro-plasma is an effective therapy for atrophic scars of facial hemangioma after isotopes radiotherapy,which can significantly improve the depression degree of scars,eliminate the hyperpigmentation and improve the color of scars,but it only has adverse reactions of wound pain and postoperative wound erythema.

Through the improvement on determination of rhTNF-? bioassay, we established the formal procedure . Methods; We choose L929 cell for rhTNF-? bioassay determination. To identify the influence of rhTNF-? on the growth of HL-60 cells, various concentration and effecting time were studied. Results: The results showed that the inhibition of rhTNF-? on HL-60 cells based on dose and time. Northern Dot Blot and DNA electrophoresis showed c-myc gene was surpressed, and DNA was damaged by rhTNF-?. Conclusion: Autometic calculation is responsible for bioassay of rhTNF-?. rhTNF-? could inhibit the proliferation of HL-60 cells and its mechanism is different from doxirubicin.

Objective: To establish National Standard of rhTNF-? complying with the requirements of research and quality control. Methods: National Standard of rhTNF-? were assayed against international standard (NIBSC) of rhTNF-? by cytotoxicity bioassay (L929 ) in vitro. The collaborative study has been carried out among four laboratories. Results: Based on the statistical analysis the results show that mean pf 95 % confidence interval is 3 289 ~ 4 266 IU per ampoule; the 95 % reference range is 1 735 ~ 8 087 IU per ampoule. Based on this study, the potency of rhTNF-? standard is defined as 3500 IU per ampoule. Stability tests indicate that the bioactivity has not been changed significandy under the storage of four different temperautres for 27 monthes. Conclusion: The preparation of rhTNF-? met the requirement of quality and could be used as a National Standard.

By using of combined HRP retrograde tracing and immunocytoche mistry methods, the projection from the caudal part of the ventrolateral medulla (VLM) to the nucleus accumbens (Acb) was examined. When HRP was injected into the ventral and medial area of the caudal part of Acb, the labeled cell bodies were mostly found in the bilateral (predominantly ipsilateral) caudal part of the VLM. When HRP technique (HRP injected into the Acb)was combined with immunocytochemical method, many HRP-TH, HRP-NT and HRP-CCK double labeled cell bodies were found in the VLM. The number of the HRP-TH double labeled cell bodies were numerous, while HRP-NT and HRP-CCK doublelabeled cells were less. HRP-TH double labeled neurons were also found in the reticular formation between the solitary tract nucleus and VLM.

The projection from the nucleus of solitary tract (Sol) to the nucleus accumbens (Acb) was examined by using HRP retrograde and anterograde tracing combined with immunocytochemical double-labeling technique. The following results were obtained: (1) when WGA-HRP was injected into the caudal part of the Sol, the labeled fibers, terminals and cell bodies were found in the ventromedial area of the caudal Acb. After injecting HRP into the ventromedial area of the caudal Acb, labeled cell bodies and terminals were found in the ipsilateral and contralateral caudal part of the Sol,mainly in the commissure nucleus and the medial subnucleus of nucleus of solitary tract. (2) After injecting HRP into the Acb and combined with immunocytochemical method, many HRP-TH, HRP-NT, and HRP-CCK double-labeled cell bodies were found in the caudal part of the Sol. The number of the HRP-TH double-labeled cell bodies was most numerous, HRP-NT cells was next and HRP-CCK cells was even less.