0.05 ). However, there was a correlation between EF at peak exercise 27%?11% and maximal exercise capacity ( r = 0.42 , P

Objective To investigate the effect of DMARDs on the prevention of early-onset atherosclerosis in rheumatoid arthritis ( RA) patients for exploring an appropriate schedule to reduce cardiovascular events. Methods Seventy-two patients with early RA were included in this study. Erythrocyte sedimentation rate ( ESR) and high sensitivity C reactive protein ( hs-CRP) were detected before and after treatment to evaluate inflammation. Carotid intima-media thickness ( cIMT) and endothelium-dependent flow-mediated vasodilatation (ED-FMD) were monitored. Disease Activity Score in 28 Joints (DAS28) and health assessment questionnaire-disability index ( HAQ-DI) was used to assess severity degree of RA and life quality of the patients, respectively. The patients were treated with methotrexate (MTX), hydroxychloroquine (HCQ) and sulfasalazine (SSZ) alone or combined based on severity of RA. Results After 1 year of treatment, ESR, hs-CRP, DAS28 and HAQ-DI were significantly improved (all P<0. 01). At the same time, the serum lipid levels also had obvious changes. However, there was no obvious difference in body mass index ( BMI) after 1 year. The cIMT ratio value was significantly decreased compared to baseline (0. 43±0. 08 mm vs. 0. 50±0. 16 mm, P=0. 002), and FMD% also significantly improved from basic value of 5. 26 to 7. 57 after treatment (P=0. 041). Conclusion Early intervention of RA by using DMARDs can effectively control the disease and slow the progression of atherosclerosis, but also reduce cardiovascular mortality.

Objective To improve the recognization of hip joint lesion in ankylosing spondylitis and it's related factors.Methods Pelvis computed radiography(CR) and HLA-B27 were examined in 100 cases of ankylosing spondylitis proved by clinical.Results 58% cases appeared differed degree of narrowing of the interticular space,osteoporosis on head of femur and acctabulum,cystis under the surface of acctabulum and joint inosculated.This probability was higner at thoe patients that had diaease at youthful or HLA-B27(+).Conclusion Hip joint lesions of ankylosing spondylitis appear characteristic signs in CR.It relates with the age that the disease come on and HLA-B27.

Objective To explore the prevalence of anxiety, depression, loneliness in elderly patients with long- term hospitalization, its coping style and the correlation between them. Methods 48 hospitalized elderly patients with long- term hospitalization were investigated by Standard Scale and self- administrated questionnaire, the Pearson's correlation was used to find the correlation. Results It was concluded that anxiety, depression and other negative emotions were commonly seen in elderly patients with long term hospitalization, these patients usually adopt the avoidance and yield coping style as well,and there was a positive correlation between them, the r value was 0.438, 1.473 amd 0.501 respectively. Conclusions This research confirmed the possibility of negative emotion in elderly patients with large long- term hospitalization, but also closely related to the choice of coping styles. The nursing staff should closely observe and assess the psychological status of elderly patients, planned, targeted to help elderly patients use more positive coping style.

To analyse the effect of tetrandrine(Tet) on proliferation of aortic vascular smooth muscle cells ( AVSMC), AVSMC were isolated and cultured from sham-operated rats(Sham), renovascular hypertensive rats〔RHR, 18 weeks after two kidney one clip(2K1C) operation〕, and Tet (50 mg*kg-1*d-1 po for 9 weeks from week 9 after 2K1C operation)treated RHR. The proliferation of AVSMC was detected by MTT method, and the DNA synthesis was evaluated by ［3H］-thymidine incorporation. The results showed that ①The ultrastructure of aorta suggested that AVSMC in RHR had transferred from contractile phenotype to synthetic phenotype; ②Compared to Sham, AVSMC from RHR showed a higher proliferative property with a higher cell number and an increased growth rate stimulated by norepinephrine(NE) or angiotensinⅡ(AngⅡ); ③Compared to untreated RHR, AVSMC from Tet treated RHR showed a reduced reactivity to NE- or AngⅡ-stimulated proliferation and growth rate; ④Tet(0.1－10 μmol*L-1) treated in vitro induced a concentration-dependent depression in ［3H］ thymidine-incorporation stimulated by NE or AngⅡ in AVSMC from either RHR or Sham. This study provides an evidence of increased reactivity to NE or AngⅡ in AVSMC of RHR. Tet inhibits the proliferation and DNA synthesis in AVSMC, depresses the susceptibility of AVSMC to AngⅡ and NE, both contribute to the regression effect on hypertensive vascular remodeling.

Objective To explore whether glycyrrhizin has protective effect on acute liver injury in mice by NF‐κB signaling pathway .Methods Totally 200 Kunming mice which the body weight were about 29 -30 g were chose and divided into 4 groups randomly ,50 cases in each group .The first group was the control group;the second group was acute liver injury group induced by CCl4 ;the third group was injected with Stronger Neo‐Minophagen C(SNMC) injection on the basis of second groups;the fourth group was treated with NF‐κB inhibitor (proDTC) on the basis of the third group .After 1 ,3 ,5 d treatment ,the serum expressing levels of ALT ,AST ,total bilirubin ,albumin of mice and prothrombin time were detected ,the Child‐Pugh score was calculated ,and the pathological observation was performed .Results The results showed that ,compared to the first group ,after CCl4 treatment ,the expressing levels of AST ,ALT ,total bilirubin and albumin ,the PT and Child Pugh score all were significantly increased(P<0 .05) , which indicated that the acute liver injury model induced by CCl4 was successfully established .After the treatment of SNMT (CCl4 +SNMT) in mice ,although the level of each index did not return to normal level ,with the extension of treatment time ,the level of each index was also significantly reduced (P<0 .05) .At the same time ,this effect could be reversed by NF‐κB inhibitor proDTC .Conclusion SNMT plays a significantly protective role in acute liver injury via regulating NF‐κB signaling pathway .

Objective To determine blood/gas and tissue/gas partition coefficients of sevoflurane, isoflurane and halothane and evaluate the effects of pregnancy on them. Methods Ten 18-22 day pregnant and 10 non-pregnant SD rats were killed under pentobarbital anesthesia. The tissue specimens of heart, liver, kidney and brain were obtained and made into homogenates respectively. The blood/gas and tissue/gas partition coefficients of sevoflurane, isoflurane and halothane were determined using a method of 2-stage headspace equilibration by gas chromatography. Results Blood/gas and brain/gas partition coefficients were lower in pregnant group than in non-pregnant group (P

Objective To observe the effect of rehabilitation on ADL scores in patients with type 2 diabetes complicated with cerebral stroke.Methods According to the selected order,three hundred and twenty type 2 diabetes patients complicated with cerebral stroke were numbered in Arabic numbers 1,2,3 and so on,the odd number patients were randomly enrolled into the observation group,the even number patients were assigned into the control group.The control group was only given corresponding clinical treatments.In addition to the same clinical treatments,rehabilitation training was given to the observation group at the same time.The ADL scores,change between the two groups were compared after three months.Results After treatment,the ADL score of the observation group was significantly improved,the difference was statistically significant (t =-24.9,P =0.000,P ＜ 0.05).The ADL score of the control group was also improved,and the difference was statistically significant (t =-15.52,P =0.000,P ＜ 0.05).The ADL score between the observation group and the control group had statistically significant difference (t =24.24,P =0.000,P ＜ 0.05).Conclusion Type 2 diabetes patients complicated with cerebral stroke who are early given rehabilitation training actively,car significantly improve the function of hemiplegia limbs,improve the ADL scores and the quality of life.

Objective:To construct BimS lentivirus RNA interference(RNAi) vector and to study its infection efficiency by using RNAi technique.Methods:Three interference targets were designed according to the BimS sequence.The single chain primer was annealed into double-stranded oligo sequences,and then connected with vector linearized with Age Ⅰ and EcoR Ⅰ enzyme.The recombinant plasmid was packaged,and the infection efficiency was observed by infecting ACC-2 cells.Results:After amplification,a 337 bp band was appeared in the electrophoresis results of positive clones.Sequence of inserted fragments were identical with the result of DNA sequencing.Restructuring lentivirus was packed in 293T cells,the virus titer was 2 × 108 TU/ml,MOI =20,and the transfection efficiency was 85％.The BmS mRNA relative expression of pFU-GV-BmS-1,pFU-GV-BMS-2 and control group was 0.743 ±0.025,0.466 ±0.023 and 1.266 ±0.042 respectively(between each 2 groups,P ＜0.05).Conclusion:BimS lentivirus virus RNA interference vectors can be constructed,and can efficiently infect ACC-2 cells.

Objective To solve the three principal problems for reusing the software components, i.e., existence, discovery and availability principles, web services provided a new solution for reusing and assembling web software or components under distributed environment with a series of XML-based protocols. Methods A 4+1 meta-model between atomic services, such as Sequence, Parallelism, Alternation, Substitute and Iteration, and a kind of service composition description language in composition process are investigated. Furthermore, an architecture-based service composition model with Ontology Engine is proposed, which is based on the software architecture life-cycle model and provides a mechanism for services composition with dynamic management and deployment. Results The results of the prototype system development show that the service-composed application not only reused the web service components, but also reused the data resources located in distributed databases. Conclusion The method of reused-based web services composition observably decreases the period and cost of development and greatly improves the programming efficiency.