Nonalcoholic fatty liver(NAFL)is characterized by the hepatocyte steatosis and lipid accumulation,and is a clinical syndrome of unrelated to excessive alcohol consumption. At present,patients with the syndrome obviously increase in the whole world. Sterol regulatory element-binding proteins (SREBPs) are the key transcription factors of controlling the biosynthesis of cholesterol,fatty acids and triglycerides,and adipocyte differentiation. In this paper,the regulation of SREBPs and its research progress in the development of NAFL were reviewed.

Objective: To study the technological parameters of the purification process of total saponins of Rubus parvifolius L. with macroreticular resin. Methods: The adsorptive characteristics and elutive parameters of the process were studied by taking the elutive and purified ratio of total saponins of Rubus parvifolius L. as marker. Results: 19.3mL of the extractive of total saponins of Rubus parvifolius L. was purified with a column of macroreticular resin (R1cm?H20cm,dried weight 2.68g) and was washed with 100mL of distilled water, then was eluted with 100mL of 50% ethanol. Conclusion: With macroreticular resin to adsorb and purify, the elutive ratio of total saponins of Rubus parvifolius L. was over 79.2% and the purity reached 55.3%. So this process of applying macroreticular resin to adsorb and purify total saponins of Rubus parvifolius L. is feasible.

Objective Constructing a hospital informatization level evaluation model and carry out an empirical research.Methods Based on experts' suggestions and comprehensive scoring method,a evaluation model of hospital informatization level has been formed,and 1,221 Chinese hospitals have been selected randomly in this empirical research.Results The evaluation model and rating scale is reliable,valid and sensitive,and it can be used to evaluate hospital informatization level at larger scope.The difference of informtization level between hospitals in different areas,different type is evident and the development situation in hospitals is imblalace also.Conclusion The hospilal should emphasis on the informatization,and improve the application of information technology.

Objective To explore the preventive effect of Weikang granule on gastric ulcer and gastric mucosa damage in rats and its mechanism. Methods Gastric ulcer model was made by stress and pylorus ligature, gastric mucosa damage was made by feeding alcohol to rats. The effect of three different dosage on the rat model were observed. Results In the three treatment groups, there is noticeable resistant effect to the formation of stress and pylorus ligature gastric ulcer of rats. Weikang granule could protect the gastric mucosa from injury caused by alcohol, increase blood flow and content of PGE2. Conclusion It may be the potential mechanism that the prescription can improve the blood circulation of the gastric wall and increase content of PGE2.

OBJECTIVE: To investigate the utilization of venous infusion before and after abolishing intravenous infusion of antibacterial drugs in second-class and upwards hospitals in Suzhou.METHODS: The current status of intravenous infusion in second-class and upwards hospitals in Suzhou in 2016 and 2017 was statistically analyzed. With hospital information system, the infusion ratio and top ten infusion drugs in the emergency, out-patients and in-patients were summarized.RESULTS: The infusion ratio was 6.33% in out-patients, 28.85% in emergency, and 83.16% in-patients.CONCLUSION: The ratio of infusion is high in second-class and upwards hospitals in Suzhou, particularly in in-patients. There are many kinds of infusion drugs and the use of high price electrolytes and adjuvant therapy infusion is increasing.

OBJECTIVE: To examine the effect of sodium valproate on lipid metabolism in children with epilepsy and investigate the potential mechanisms. METHODS: The body mass index(BMI), the levels of serum total cholesterol(TC), triglyceride(TG), low density lipoprotein-cholesterol(LDL-C), high density lipoprotein-cholesterol (HDL-C), LDL-C/HDL-C, apolipoprotein AI(ApoAI) and apolipoprotein B(ApoB) of epilepsy children treated with sodium valproate after different time were measured.The gene expression of PPARγ in children's peripheral blood was examined by Realtime-PCR. RESULTS: The epilepsy children's BMI, the levels of serum TC, TG, LDL-C and AproB were increased(P<0.05 or P<0.01) after treatment with sodium valproate different time.The liver function has significant difference between untreated epilepsy children and epilepsy children treated with sodium valproate more than one year. Sodium valproate could significantly increase the mRNA expression of PPARγ(P<0.05) in epilepsy children. CONCLUSION: Sodium valproate has effect on lipid metabolism in epilepsy children and the mechanisms might be associated with its regulation of the PPARγ mRNA expression.

Objective A method was established for genotyping of hepatitis B virus (HBV A-D genotype),based on the PCR-restriction fragment length polymorphism (RFLP) created by Hinf Ⅰ,Ear Ⅰ,Apo Ⅰ action on an amplified segment of the S region.Methods Clinical diagnosis research.One hundred and twenty-eight HBV S sequences obtained from GeneBank were analyzed for restriction enzyme sites that would be genotype-specific.Restriction patterns following digestion with restriction enzymes Hif Ⅰ,Ear Ⅰ,Apo Ⅰ were determined to identify A-D HBV genotypes.The method was used to detect the HBV genotype of fifty severe hepatitis patients due to chronic hepatitis B in China.Then the detection results were confirmed by direct sequencing.Results The new genotyping method was established,named simple PCR-RFLP,which could identify HBV genotypes A to D.Genotypes B,C,B/C and A or D could be determined by a single step digestion with Hif Ⅰ.Eight patients of genotype A/B/C classified by single step digestion with Hif Ⅰ were conformed as genotype B variant by further digestion and direct sequencing.Extracted randomly and diluted into different concentration,three specimens were tested for genotype of HBV repeatedly and respectively.The results were all in accord with the originals,and the lowest detection limit of HBV DNA was 7 ～ 9 IU/ml.This was particularly useful in China where genotypes B and C were predominant.Twenty-three of genotype B and ten genotype C patients were classified from these fifty severe hepatitis B patients by a single step digestion with Hif Ⅰ through the simple PCR-RFLP method.The same results were also obtained by direct sequencing of PCR products (Kappa =1.00,P =0.001).The simple PCR-RFLP method was superior to direct sequencing in detecting HBV B/C polyinfection (9 cases and 0 case; x2 =18.00,P =0.001).Conclusions Both the sensitivity and repetitiveness of Simple PCR-RFLP method are satisfactory.It is superior to direct sequencing in detecting HBV B/C polyinfection,and simple,convenient.

Neurons descending from the griseum centrale mesencephali, nucleus Darkschewitsch and nucleus interstitialis of Cajal to the nucleus raphe magnus and adjacent reticular formation (nucleus reticularis gigantocellularis and nucleus reticularis pontis caudalis) were identified in 9 adult cats with the retrograde HRP method. In the griseum centrale mesencephali, the labeled neurons were found bilaterally but slightly more ipsilaterally. In the nucleus Darkschewitscb and nucleus interstitialis of Cajal, the labeled neurons were consistently found in its rostral part ipsilateral to the injected side at the level of the posterior commissure. In addition, in 5 of the 9 cases, a few labeled neurons were observed in the nucleus raphe dorsalis.

Objective To investigate the drug resistance situation and the epidemiological characteristicin the tuberculoupatientin ordeto provide the reference foeffective prevention and treatmenof T.MethodTotally 2 271 patientwith posi-tive sputum smeapulmonary tuberculosi(TB) in the Jiangbei Branch Hospital of Chongqing Zhongshan Hospital from January 2009 to Decembe2013 were performed the drug resistance detection of 10 kindof anti-Tdrugs(isoniazid ,streptomycin ,rifampi-cin ,ethambutol ,para-aminosailcyliacid ,kanamycin ,pasiniazid ,protionamide ,capreomycin ,and ofloxacin) .ResultOf all 2 271 ca-seof positive sputum culture pulmonary T,the total drug resistance rate and total multi-drug resistance rate were 61 .91% and 26 .29% ,respectively ;the total drug resistance ratein initial treatmenand retreatmenpatientwere 52 .85% and 78 .06% respec-tively ,and the multi-drug resistance ratewere 16 .70% and 43 .38% ,respectively ;the top five in the average drug resistance ratefrom high to low were streptomycin(39 .45% ) ,isoniazid(33 .20% ) ,rifampicin(32 .94% ) ,para-aminosailcyliacid(31 .40% ) and pasiniazid(21 .53% );the anti-Ttreatmenhistory ,gende,age and occupation were significantly associated with the drug resist-ance occurrence (P＜0 .05) .Conclusion The drug resistance situation of the Tpatientin the local areof Chongqing iseriouin the recen5 year.The standardized managemenand treatmenon the Tpatientwith drug resistance should be strengthened .