Objective To observe the effect of herbs of cleaning away heat,drying dampness and invigorating spleen on insulin resistance and TNF-? of the patients with early stage of type 2 diabetes mellitus.Method Eighty-eight patients with type 2 diabetes mellitus were randomly divided into 2 groups.Forty-four cases of the treatment group were treated with herbs of cleaning away heat,drying dampness and invigorating spleen,and 44 cases of the control group were treated with metformin.The data of their weight,glucose,insulin and TNF-? before and after treatment was compared.Results Glucose and insulin of two groups declined(P

Objective To analyze the effect of prognosis of psychological counseling combined with early double anti antiplatelet on the treatment of acute cerebral infarction.Methods 76 patients with acute cerebral infarction who were admitted to our hospital from February 2014 to March 2016 were numbered according to their admission order.The patients with even numbers were in the observation group and with the odds number in the control group, 38 cases in each group.The control group were given aspirin and clopidogrel in the early stage, and the observation group were given psychological guidance on the basis of the control group.The prognosis in the two groups was recorded and analyzed.Results The neurological deficit score (NIHSS) and simple mental state score (MMSE) in the observation group (9.16±0.73)points, (25.42±2.00)points were significantly higher in the observation group than those in the control group (12.59±1.05)points, (20.73±2.16)points.And the differences were significant (P<0.05).The level of dailyliving activity index (Barthel) and Hamilton depression (HAMD) in the observation groupwere (80.86±3.29)points, (13.70±1.53)points, respectively, which were better than those in the control group (71.53±3.75)points, (18.53±1.99)points, and the differences were significant (P<0.05).The total adverse reaction rate in the treatment group was 18.42%, slightly higher than 14.29% in the control group, but the difference was not statistically significant.Conclusion Psychological counseling combined with early double antiplatelet intervention has positive effect on the prognosis in the patients with acute cerebral infarction.

Objective: To compare the volatile constituents of folium artemisiae argyi in order to provide chemical evidence for quality standardization. Methods: Volatile oil was extracted from folium artemisiae argyi from five sources by vapour distillation and then detected by gas chromatography-mass spectrometer (GC-MS) to identify and semi-quantify its chemical constituents. Results: The folium artemisiae argyi in Qichun (Hubei Province) and Anguo (Hebei Province) contained many volatile constituents, with uniform and stable contents of these volatile constituents. Conclusion: The types and contents of volatile oil of folium artemisiae argyi are varied in different sources.

Objective To explore the development requirements and tentative ideas on the standardized teaching wards in the internship course of undergraduate nursing students. Methods 134 nursing undergraduate students before the internship and 70 nursing teachers were recruited in this study. A self-made questionnaire was distributed to these students and teachers to explore their requirements and needs in the development of the standardized teaching wards. Results The majority of students and nursing teachers thought that it was necessary to design and develop the standardized teaching wards for the nursing students and the six items of this study were accepted by them. Conclusion The standardized teaching wards will be designed and developed according to the six items of this study.

Objective To find whether heNOS could be expressed in mouse lung after intratracheal administration of recombinant adenovirus vector.Methods heNOS cDNA was obtained by RT-PCR from total RNA which extracted from human HUVEC.The replication-deficient heNOS recombinant adenovirus vector was constructed with a ligation method.High titer of recombinant adenovirus was obtained by chromatographic methods.The expression of heNOS protein was determined by immunohistochemistry staining after intratracheal administration of recombinant adenovirus.Results Sequence confirmed the cloned cDNA containing the whole ORF and the heNOS cDNA was about 3731 bp.It showed 99.93% identity with that of heNOS cDNA in GenBank(163729).The biological activity of heNOS protein was examined in transfected cos7 cell line with heNOS cDNA in eukaryotic expression vector of pcDNA3.0.The replication-deficient recombinant adenovirus vector containing heNOS cDNA was constructed successfully and the purified viral titer was 2.0?1010pfu/mL.After intratracheal administration of the recombinant adenovirus,heNOS expression was found in the majority of bronchial epithelium,alveolar lining cells,endothelial cellsand smooth muscle cells of pulmonary vessels.In control group,little endogenous eNOS immunoreactivity was detected in pulmonary vessels and no eNOS immunoreactivity was shown in bronchial and alveolar epithelial cells.Conclusion The replication-deficient recombinant human endothelial nitric oxide synthase mediated by adenovirus vector constructed could be delivered into the lung tissue of mouse by intratracheal administration of recombinant adenovirus and can be expressed in lung tissue with high-efficiency.

BACKGROUND: As a method to effectively improve organic tolerance and velocity, altitude training has been gradually accepted by the coaches,and it is a problem to be solved to find the optimal altitude.OBJECTIVE: To establish rat models of stimulating altitude training, and observe the changes of myocardial cell structure in the altitude training, so as to provide scientific animal models for the physiological studies of altitude training, and provide more scientific theoretical evidence for the altitude training of athletes.DESIGN: Based on the division of altitude region in aerospace medicine,the experimental animals were divided into different altitude training groups and control groups. Morphological characters of cellular and subcellular structures were observed under electron microscope, the biochemical analysis of cardiac troponin T were performed, and then the myocardial injury was completely analyzed from two pathways of morphology and biochemistry.SETTING: Laboratory of Sports and Human Sciences, Tsinghua University.MATERIALS: The experiments were carried out in the Laboratory of Sports and Human Sciences, Tsinghua University from January 2000 to December 2002. Totally 372 male SD rats were used. The experimental materials included hypobaric oxygen chamber, Japanese H-500 transmission electron microscope and the kit for testing serum cardiac troponin T index.METHODS: After adaptive swimming training for 1 week, all the 372 rats were randomly divided into 9 groups and 54 subgroups, only 6 groups of them were analyzed in this experiment, including stimulating acute altitude hypoxia training at 4 000 m group (n=32), hypoxia without training group (n=24), stimulating acute altitude training at 4 000 m followed by reoxygen training for 1 week group (n=32), stimulating acute altitude training at 4000 m followed by reoxygen training for 2 weeks group (n=32), increasing altitude training group (n=32) and plain training control group (n=24). Rats in the plain training control group lived and trained in normal conditions,and those in the other groups all trained with swimming in the stimulating altitude environment for 4 weeks. They were trained for 7 days every week,once a day and 60 minutes for each time for 4 weeks. The serum cardic troponin T index of rats was detected and observed with enzyme-linked immunoabsorbent assay (ELISA). After stimulating acute altitude training at 4 000 m for 4 weeks followed by reoxygen training for 2 weeks, the rats were killed, and a small piece of myocardial tissue was taken from left and right ventricles, then double-stained with uranyl acetate and lead citrate,and the ultrastructure of the sections was observed with Japanese H-500 transmission electron microscope.MAIN OUTCOME MEASURES: The serum cardiac troponin T index of rats trained at different altitude, and the results of spectrum and observation of myocardial ultrastucture under electron microscope were mainly observed.RESULTS: ① Changes of body mass: The myocardial increasing rate in the hypoxia training group was significantly different from those in the hypoxia without training control group and plain training control group (P ＜ 0.01), and there was also significant difference between the latter two groups (P ＜ 0.05). The increasing rate of body mass was significantly different among the hypoxia training group, hypoxia without training control group and plain training control group (22.41％, 28.14％, 33.48％,P ＜ 0.01-0.05). ② Results of cardiac troponin T: Compared with the hypoxia without training group, the value of cardiac troponin T in the hypoxia training groups was significantly different, and significantly increased with weeks of training (P ＜ 0.01). During the hypoxia altitude training below 4 000 m for 1-4 weeks, the value of cardiac troponin T ranged in creased from 0.09 to 0.128 μg/L, and it gradually recovered to 0.09 μg/L after reoxygen training in plain for 2 weeks. ③ The results of observation under electron microscope showed that after acute hypoxia training at 4 000 m for 4 weeks, myocardial mitochondrial structure occurred mild swelling with the prolongation of training, and for the rats received reoxygen training in plain for 2 weeks, the thick and thin myofilaments of myocardial fibers formed light and dark bands were clear and distinct, and the myotomes arranged orderly, besides clustered or scattered glycogen granules, a great amount of mitochondria also existed among muscular bundles, no swelling, degeneration of vacuoles, lysis necrosis, interstitial edema and other pathological changes were observed.CONCLUSION: It is safe to train rats at any altitude below 4 000 m. The acute exercise training of great intensity directly at 4 000 m will cause the mild injury of cardiac subcellular structure, and the increase of cardiac troponin T concentration. The training by means of increasing altitude has slighter influence on the myocardial injury, and the recovery after training is also faster.

Objective: To explore the characteristics of IR spectra of the crude drug Herba Sedi collected in different habitats and seasons.Methods: The infrared spectra of all the samples of the whole plants were obtained by FT-IR spectroscopy,the sample powder tablets were used,and identifi cation features were determined through comparative study.Results: The harvest seasons of the samples are remarkly related to the positions(cm-1),intensity or ratios of some major absorbing peaks of the infrared spectra from the 15 samples of several different months and 4 habitats in Hubei Province,China.As for peak positions,the peak values(1625?7)cm-1 of all the samples in spring are less than 1623cm-1,but in autumn(April to October) are generally more than 1628 cm-1.And peak values(1057?16)cm-1 of the samples of spring are more than 1050 cm-1,but less than 1050 cm-1 for the samples of summer and autumn.Moreover,the habitats of samples are also related to IR spectra to some certain extent.Conclusion: The harvest seasons of Herba Sedi from Hubei Province can be preliminarily ascertained according to the IR spectra features.IR technique should be paid attention to in research and identifi cation of the harvest periods of crude plant drugs.

0. 5) in the patients with nephritis, uretericcalculi and other diseases. The renogram methodis simple, inexpensive, without blood samplingand is useful in clinical application.

Objective To investigate relationship between microRNA-200c and ZEB1 in cervical epithelial mesenchymal transition factor. Methods 50 cases diagnosed with cervical cancer whose tumor tissue were taken to be the experiment group.50 cases diagnosed with uterine myoma whose normal tissue were taken to be the control group.ZEB1 and microRNA-200c of uterine tissue were were detected.Results The average level of ZEB1 in the experimental group was significantly higher than that of the control group (P<0.05), and in cervical cancer, lymph node metastasis and the depth of myometrial invasion had statistically significant (P<0.05).In experimental group, microRNA-200c average was lower than control group (P<0.05), and in cervical cancer, different pathological grade group, lymph node metastasis and deep muscular layer infiltration had statistically significant(P<0.05).In experimental group, ZEB1 and microRNA-200c showed negative correlation(r =-0.270 P =0.001).Conclusion The expression level of ZEB1 in cervical cancer is higher, and the expression level of microRNA-200c in cervical cancer is lower, suggesting that the two factors may play an important role in the invasion and metastasis of cervical cancer.The correlation between the two factors suggests that microRNA-200c may be involved in the process of cervical cancer by ZEB1.

Objective:To compare therapeutic effects of hemodiafiltration (HDF) ,hemoperfusion combined hemodi‐alysis (HP+ HD) and high flux hemodialysis (HFHD) on uremic refractory hypertension (URH) .Methods :A total of 60 URH patients undergoing maintenance hemodialysis (MHD) were selected from three hospitals .They were randomly and equally divided into HDF group ,HP+ HD group and HFHD group .The 24h ambulatory blood pres‐sure ,concentrations of parathyroid hormone (PTH) andβ2‐microglobulin (β2‐MG) were detected and compared a‐mong three groups before ,after first treatment and 12 weeks after treatment .Results:Compared with before treat‐ment ,after the first treatment ,there were significant reductions in blood pressure ,blood concentrations of PTH andβ2‐MG in three groups , P< 0.05 or <0.01 ,and therapeutic effect was most significant in HP + HD group ;compared with after first treatment ,12 weeks later ,blood concentrations of PTH and β2‐MG further significantly reduced in three groups (P<0.05 or <0.01) ,compared with HDF group and HFHD group ,there was significant reductions in blood concentrations of PTH [(211.12 ± 50.05) pg/L ,(195.49 ± 48.32) pg/L vs .(75.43 ± 14.38) pg/L] andβ2‐MG [ (9.16 ± 5.02) mg/L ,(9.92 ± 5.37) mg/L vs .(5.39 ± 2.09) mg/L] in HP+ HD group , P<0.01 all .In HDF、HP+ HD、 HFHD groups ,the DBP were [ (86.70 ± 12.76) mmHg)、 (86.10 ± 9.84) mmHg)、(90.95 ± 10.15) mmHg] respectively ,those were return to normal mainly ,and no significant difference ,the SBP were [ (140.75 ± 10.85) mmHg)、(146.20 ± 8.31) mmHg)、(146.30 ± 9.65) mmHg) ,those were close to normal and no significant difference also .Adverse reactions were mild in three groups ,that of HDF group was significantly less than those of HP+ HD group and HFHD group [0 vs .40% ,45% , P<0.05 all] .Conclusion:HDF ,HP+ HD and HFHD could effectively remove blood PTH and β2‐MG ,and lower blood pressure in URH patients .Hospital may choose therapeutic plan according to specific needs of patients and HD room condition .