Objective: To compare the volatile constituents of folium artemisiae argyi in order to provide chemical evidence for quality standardization. Methods: Volatile oil was extracted from folium artemisiae argyi from five sources by vapour distillation and then detected by gas chromatography-mass spectrometer (GC-MS) to identify and semi-quantify its chemical constituents. Results: The folium artemisiae argyi in Qichun (Hubei Province) and Anguo (Hebei Province) contained many volatile constituents, with uniform and stable contents of these volatile constituents. Conclusion: The types and contents of volatile oil of folium artemisiae argyi are varied in different sources.

Objective To observe the effect of herbs of cleaning away heat,drying dampness and invigorating spleen on insulin resistance and TNF-? of the patients with early stage of type 2 diabetes mellitus.Method Eighty-eight patients with type 2 diabetes mellitus were randomly divided into 2 groups.Forty-four cases of the treatment group were treated with herbs of cleaning away heat,drying dampness and invigorating spleen,and 44 cases of the control group were treated with metformin.The data of their weight,glucose,insulin and TNF-? before and after treatment was compared.Results Glucose and insulin of two groups declined(P

Objective To analyze the effect of prognosis of psychological counseling combined with early double anti antiplatelet on the treatment of acute cerebral infarction.Methods 76 patients with acute cerebral infarction who were admitted to our hospital from February 2014 to March 2016 were numbered according to their admission order.The patients with even numbers were in the observation group and with the odds number in the control group, 38 cases in each group.The control group were given aspirin and clopidogrel in the early stage, and the observation group were given psychological guidance on the basis of the control group.The prognosis in the two groups was recorded and analyzed.Results The neurological deficit score (NIHSS) and simple mental state score (MMSE) in the observation group (9.16±0.73)points, (25.42±2.00)points were significantly higher in the observation group than those in the control group (12.59±1.05)points, (20.73±2.16)points.And the differences were significant (P<0.05).The level of dailyliving activity index (Barthel) and Hamilton depression (HAMD) in the observation groupwere (80.86±3.29)points, (13.70±1.53)points, respectively, which were better than those in the control group (71.53±3.75)points, (18.53±1.99)points, and the differences were significant (P<0.05).The total adverse reaction rate in the treatment group was 18.42%, slightly higher than 14.29% in the control group, but the difference was not statistically significant.Conclusion Psychological counseling combined with early double antiplatelet intervention has positive effect on the prognosis in the patients with acute cerebral infarction.

Objective To construct the eukaryotic expression vector with the mutant of hypoxia inducible factor 1α (HIF-1α) that could over express nonhypoxic dependent HIF-1α and investigate its expression in eukaryotic cells PC12. Methods The coding sequence of HIF-1α was amplified from total RNA from the injured spinal cord of rats by means of RT-PCR. Then, overlapping extension PCR was employed to obtain one complete sequence encoding HIF-1α mutant without oxygen-sensitive degradation domain (ODD). By using gene recombination technique, the gene HIF-1α△ODD was inserted into eukaryotic expression vector _pEGFPC1 that contained a reporter gene of enhanced green fluorescent protein. The recombinant plasmid was successfully constructed and transfected into the neural cell line PC12. The expression of HIF-1α△ODD was analyzed by fluorescent observation and Western blot. Results A 2kb gene fragment encoding H1F-1α△ODD was successfully obtained by overlapping extension PCR. On this basis, the recombinant plasmid _pEGFPC1-HIF-1 α△ODD was constructed and testified with restriction enzyme digestion and DNA sequencing. After this recombinant plasmid was transfected into the neural cell line PCI2, both fluorescent observation and Western blot demonstrated that HIF-1α△ODD was over expressed under nonhypoxia conditions. Conclusion The eukaryotic expression vector _pEGFPC1-HIF-1α△ODD is successfully constructed and HIF-1α△ODD over expresses in transfected PCI2 cell lines.

Adolescent ; Female ; Humans ; Intracellular Signaling Peptides and Proteins ; genetics ; Lymphohistiocytosis, Hemophagocytic ; genetics ; Mutation ; Signaling Lymphocytic Activation Molecule Associated Protein

Objective To investigate the circulatory levels of hormones and cytokines in the patients with the chronic heart failure(CHF)and the effect of naftopidil.Methods The levels of tumor necrosis factor-?(TNF-?),interleukin-1(IL-1),interleukin-6(IL-6),endothelin-1(ET-1),adrenomedullin(Adm),neuropeptide Y(NPY),calcitonin gene-related peptide(CGRP),atrial natriuretic peptide(ANP),brain natriuretic peptide(BNP)and C-type natriuretic peptide(CNP),and left ventricular fraction(LVEF)of 47 patients with CHF(CHF group)and 24 healthy subjects(control group)were measured by radioimmunoassay or echocardiography respectively.The patients were randomized to receive naftopidil(50mg Bid)in addition to routine therapy(B group,n=23)or routine therapy only(A group,n=24)for one-month.Results (1)The levels of TNF-?,IL-1,IL-6,ET-1,Adm,NPY,ANP,BNP and CNP of the CHF group were higher(P 0.05)in the levels of IL-1,IL-6,Adm,CNP and LVEF.(4)There were significantly inverse correlations between LVEF and TNF-?,ET-1,IL-6 or BNP(P

Objective: To explore the characteristics of IR spectra of the crude drug Herba Sedi collected in different habitats and seasons.Methods: The infrared spectra of all the samples of the whole plants were obtained by FT-IR spectroscopy,the sample powder tablets were used,and identifi cation features were determined through comparative study.Results: The harvest seasons of the samples are remarkly related to the positions(cm-1),intensity or ratios of some major absorbing peaks of the infrared spectra from the 15 samples of several different months and 4 habitats in Hubei Province,China.As for peak positions,the peak values(1625?7)cm-1 of all the samples in spring are less than 1623cm-1,but in autumn(April to October) are generally more than 1628 cm-1.And peak values(1057?16)cm-1 of the samples of spring are more than 1050 cm-1,but less than 1050 cm-1 for the samples of summer and autumn.Moreover,the habitats of samples are also related to IR spectra to some certain extent.Conclusion: The harvest seasons of Herba Sedi from Hubei Province can be preliminarily ascertained according to the IR spectra features.IR technique should be paid attention to in research and identifi cation of the harvest periods of crude plant drugs.

Objective To analyze and evaluate the safety and efficacy of modified Pie-crusting ( PC) technique for releasing medial tightness during primary total knee arthroplasty ( TKA) . Methods We completed primary TKA by the same performer with modified PC technique in 30 patients (34 knees) with genu varus from March 2014 to June 2016.By using a special curved scalpel with width limit of 3 mm and depth limit of 5 mm to poke the tension parts , we released the anterion bondle of superficial medial collateral ligament ( sMCL) and posteromedial corner structures ( PMCS) during tension happened in extension , and we released the posterior bondle of sMCL during tension happened in flexion .According to the gap value measured intraoperatively , we divided these cases into three groups: extension with flexion tension group ( 10 knees ) , extension tension group ( 13 knees ) , and flexion tension group (11 knees).The difference between medial and lateral gap value no more than 1 mm was defined as gap balance.We calculated the gap balance rate of each group .Series of weighted frontal X-ray were conducted at fixed period to evaluate the varus angle of the knee postoperatively.The range of motion (ROM), HSS scores and WOMAC scores were also recorded at the same time . Results Among the 34 knees , 31 knees reached the medial and lateral gap balance at both extension and flexion .There was a difference of 2 mm in medial and lateral gap value at extension in 1 knee and the same difference at flexion in the other 2 knees.The total postoperative gap balance rate was 91.2%( 31/34 ).The constrained inserts were implanted in 3 cases.No technical-related complications happened after the surgery.After the releasing procedure, the flexion gap value had an increase of 1 mm (range, 1-3mm) in the extension tension group , and the extension gap value had an increase of 1 mm ( range, 1-2 mm) in the flexion tension group, without significant difference (Z=-1.118, P=0.264).The ROM was 83.3°±14.7°preoperatively and 100.7°±14.2° postoperatively (t=-7.714, P=0.000).The median alignment of the knee was 11.5°(range, 7°-32°) preoperatively and 1° (range, 0°-4°) postoperatively (Z=-5.092,P=0.000).The HSS scores were (42.7 ±16.3) points preoperatively and (88.1 ± 9.9) points postoperatively (t=-21.868, P=0.000).The WOMAC scores were (76.2 ±8.2) points preoperatively and (11.4 ± 9.7) points postoperatively (t=31.726, P=0.000).All of them were significantly improved in comparison with those before the surgery. Conclusions Using modified PC technique is safe and effective in medial releasing during primary TKA .Both extension and flexion gap value will be affected by releasing tensed fiber at extension or flexion position .

Objective:To compare therapeutic effects of hemodiafiltration (HDF) ,hemoperfusion combined hemodi‐alysis (HP+ HD) and high flux hemodialysis (HFHD) on uremic refractory hypertension (URH) .Methods :A total of 60 URH patients undergoing maintenance hemodialysis (MHD) were selected from three hospitals .They were randomly and equally divided into HDF group ,HP+ HD group and HFHD group .The 24h ambulatory blood pres‐sure ,concentrations of parathyroid hormone (PTH) andβ2‐microglobulin (β2‐MG) were detected and compared a‐mong three groups before ,after first treatment and 12 weeks after treatment .Results:Compared with before treat‐ment ,after the first treatment ,there were significant reductions in blood pressure ,blood concentrations of PTH andβ2‐MG in three groups , P< 0.05 or <0.01 ,and therapeutic effect was most significant in HP + HD group ;compared with after first treatment ,12 weeks later ,blood concentrations of PTH and β2‐MG further significantly reduced in three groups (P<0.05 or <0.01) ,compared with HDF group and HFHD group ,there was significant reductions in blood concentrations of PTH [(211.12 ± 50.05) pg/L ,(195.49 ± 48.32) pg/L vs .(75.43 ± 14.38) pg/L] andβ2‐MG [ (9.16 ± 5.02) mg/L ,(9.92 ± 5.37) mg/L vs .(5.39 ± 2.09) mg/L] in HP+ HD group , P<0.01 all .In HDF、HP+ HD、 HFHD groups ,the DBP were [ (86.70 ± 12.76) mmHg)、 (86.10 ± 9.84) mmHg)、(90.95 ± 10.15) mmHg] respectively ,those were return to normal mainly ,and no significant difference ,the SBP were [ (140.75 ± 10.85) mmHg)、(146.20 ± 8.31) mmHg)、(146.30 ± 9.65) mmHg) ,those were close to normal and no significant difference also .Adverse reactions were mild in three groups ,that of HDF group was significantly less than those of HP+ HD group and HFHD group [0 vs .40% ,45% , P<0.05 all] .Conclusion:HDF ,HP+ HD and HFHD could effectively remove blood PTH and β2‐MG ,and lower blood pressure in URH patients .Hospital may choose therapeutic plan according to specific needs of patients and HD room condition .

Objective To investigate relationship between microRNA-200c and ZEB1 in cervical epithelial mesenchymal transition factor. Methods 50 cases diagnosed with cervical cancer whose tumor tissue were taken to be the experiment group.50 cases diagnosed with uterine myoma whose normal tissue were taken to be the control group.ZEB1 and microRNA-200c of uterine tissue were were detected.Results The average level of ZEB1 in the experimental group was significantly higher than that of the control group (P<0.05), and in cervical cancer, lymph node metastasis and the depth of myometrial invasion had statistically significant (P<0.05).In experimental group, microRNA-200c average was lower than control group (P<0.05), and in cervical cancer, different pathological grade group, lymph node metastasis and deep muscular layer infiltration had statistically significant(P<0.05).In experimental group, ZEB1 and microRNA-200c showed negative correlation(r =-0.270 P =0.001).Conclusion The expression level of ZEB1 in cervical cancer is higher, and the expression level of microRNA-200c in cervical cancer is lower, suggesting that the two factors may play an important role in the invasion and metastasis of cervical cancer.The correlation between the two factors suggests that microRNA-200c may be involved in the process of cervical cancer by ZEB1.