Objective To define the expression of AQP4 in normal rat eyes at transcriptional and translational level, so as to provide morphological bases for the mechanisms of water transport in eyes. Methods Immunohistochemical SABC method and in situ hybridization were used to detect the expression and localization of AQP4 in normal rat eyes. Results The corneal epithelium, the iridal pigment epithelium cells, the nonpigment epithelium cells of ciliary body, the lens epithelium cells, the lens fiber cells, the outer nuclear layer cells, and the inner nuclear layer cells, the ganglion cells of the retina showed positive immunoreactivity of AQP4. The reactive substance was distributed in membrane but not in nuclei of all positive cells. The resuIts of in situ hybridization were identical with that of immuohistochemistry method. AQP4 mRNA could be detected in the cytoplasm of positive immunoreactive cells, however, nuclei were negative. Conclusion The results implied that AQP4 could coordinate with other AQP4 to play an important role in the secretion and regulation of aqueous humor, the maintenance of the diaphaneity of cornea and lens, the motivation and transmission of optesthesia.;

OBJECTIVE To study the therapeutic effect of ceftazidime(CAZ) in a rat model of pneumonia caused by CTX-M-14 extended-spectrum ?-lactamases(ESBLs)-producing Klebsiella pneumoniae which is susceptible to ceftazidime in vitro.METHODS Forty eight rats were divided into four groups randomly which were ceftazidime-treated group,piperacillin/tazobactam-treated group,ceftaxime-treated group and the control group.All rats were intranasally inoculated with bacterial suspension of CTX-M-14 ESBLs-producing K.pneumoniae to produce pneumonia models.Twenty four hours after inoculation,rats were intramuscularly administered ceftazidime,piperacillin/tazobactam,ceftaxime,or saline,respectively.The temperature,leucocyte count,percentage of neutrophils,and bacterial counts in the right lungs of rats were detected at some timepoints.RESULTS Compared with the ceftaxime-treated group and the control group,the leucocyte count,percentage of neutrophil count and common logarithm of the bacterial count in the right lungs of the ceftazidime-treated group and piperacillin/tazobactam-treated group after the treatment which lasted seventy two hours were significantly lower than those of ceftaxime-treated group and the control group(P0.05).CONCLUSIONS Ceftazidime shows good therapeutic effect in pneumonia in rats caused by CTX-M-14 ESBLs-producing K.pneumoniae which is susceptible in vitro.

This paper briefly introduced the characteristics, origin and development of virtual reality, and the value for rehabilitation; reviewed the application of virtual reality in the rehabilitation of balance function after stroke. Some assessment commonly used for balance function were summarized.

OBJECTIVETo observe the efficacy of encephalopathy therapeutic tongue acupoint apparatus (ETTAA) in the treatment of autism.

METHODSEighty-four children of autism were randomly divided into a tongue acupuncture group (group A) and a conventional training group (group B), 42 cases in each group. The behavior training and sensory integration training were carried out in group B and the ETTAA was added in group A. The apparatus was switched on for 20 min every time and 3 times a day. Treatment of two months were carried out in both groups. The score of childhood autism rating scale (CARS) and clinical efficacy in both groups were observed before and after treatment.

RESULTSAfter treatment, the CARS in both groups were significantly reduced (42.39 +/- 6.86 vs 32.15 +/- 5.12, P < 0.001; 44.58 +/- 6.76 vs 39.72 +/- 7.11, P < 0.05), which was more significant in group A (P < 0.01). The totally effective rate in group A [90.5% (38/42)] was superior to that in group B [66.7% (28/42), P < 0.01].

CONCLUSIONThe clinical efficacy in tongue acupuncture group is apparently superior to that in conventional training group, ETTAA combined with conventional training have a better curative effect in the treatment of autism.

Acupressure ; Acupuncture Points ; Autistic Disorder ; therapy ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Tongue ; anatomy & histology

Language ; Periodicals as Topic ; Publishing

OBJECTIVETo analyze the status and papers information of Chinese Journal of Applied Physiology, and provide a guide for authors, readers and editing staffs.

METHODSWe retrieved papers of Chinese Journal of Applied Physiology from 2009 to 2013 from CNKI, and performed a statistical analysis of papers information contained in the text using bibliometrics method. Papers information such as document type, found support, author affiliation type, and discipline classification, etc. was analyzed.

RESULTSSeven hundred and forty two papers including 27 full English articles in total were published in Chinese Journal of Applied Physiology from 2009 to 2013. More papers were supported by national, provincial, and the other foundation, it was about 82.6% of all papers.

CONCLUSIONChinese Journal of Applied Physiology has stable manuscript resources, various discipline papers, and play an important role for development of applied physiology in China.

Bibliometrics ; China ; Periodicals as Topic

Objective The dynamic accumulation trend of secondary metabolite in cultivated Spatholobus suberectus Dunn at different growth time was studied to provide scientific basis for confirming its reasonable harvesting time. Methods Ethanol-soluble extractives were measured by the method recorded in Chinese Pharmacopoeia ( A) Appendix XA ( 2010 edition). The content of total flavonoids was tested by ultraviolet spectrophotometer. The contents of protocatechuic acid, catechin, and epicatechin in Spatholobus suberectus Dunn were determined by high performance liquid chromatography ( HPLC). Results The contents of ethanol-soluble extractives, total flavonoids and the major components such as protocatechui cacid, catechin and epicatechin in Spatholobus suberectus Dunn showed increasing trend with the prolongation of growth time. The changes of contents of alcohol-soluble extract, total flavonoids and epicatechin in samples collecting at different months in one year showed a parabolic trend, and the contents reached the peak values from July to September. Catechin content kept increasing continually. The content of ethanol -soluble extractives of Spatholobus suberectus Dunn aged 4-6 years met the requirements of the current edition of Chinese Pharmacopoeia when harvested from July to September. Conclusion It is preliminarily recommended that Spatholobus suberectus Dunn cultivated for more than 7 years is suitable for harvesting, but the optimal harvest period and the harvest month still need to be further studied.

Objective This study was designed to determine the clinical pharmacokinetics ofcisatracurium in Chinese patients. The purpose of this study was twofold: the determination of ED50 and ED95 of cisatracurium and the intubating conditions and changes in BP and HR produced by 2×ED95 and 3 ×ED95 . Methods 100 patients scheduled for elective surgery, aged 30-65 years, served as the experimental subjects. Anesthesia was induced with propofol 2mg/kg and fentanyl 4μg/kg. 5 min after BP and HR were stabilized the study was started. The study consisted of two parts according to the purpose of the study. In part Ⅰ 70 patients were included and randomized to 7 groups based on the doses of cisatracurium the patients received. The doses were 25, 30, 35, 40, 45, 50 and 55 μg/kg from group Ⅰ to Ⅶ. Neuromuscular transmission was assessed by measuring the responses of adductor pollicis muscle to stimulation of ulnar nerve with TOF pulses.The maximal depression of Tt was measured after administration of cisrtracurium, and ED50 and ED95 were calculated. In part Ⅱ 30 patients were included and divided into two groups to receive 2×ED95 (group Ⅷ ) or 3×ED95 (group Ⅸ ) of cisatracurium. The intubating conditions were evaluated. The onset time and time of recovery from paralysis as well as the changes in BP and HR after administration of cisatracurium were recorded. ResultsED50 and ED95 for cisatracurium were 30.7μg/kg and 53.4μg/kg respectively. The onset times for 2×ED95 and 3×ED95 were (254±84)s and (190±59)s. The durations of action was (38.8±6.1)min and (53.28.8)min respectively. Recovery indexes were (16.4±4.9) min and (15.6±5.2) min. There were little changes in BP and HR after administration of cisatracurium in both group Ⅷ and Ⅸ. The intubating conditions were not significantly different. ConclusionsED50 and ED95 for cisatracurium are 30.7μg/kg and 53.4μg/kg in Chinese patients. The onset time is shorter and duration of action longer with 3×ED95 which does not significantly affect BP and HR.

OBJECTIVE To investigate the procedures and advantages of whole range quality control of disposable sterile medical appliance. METHODS By analyzing the data concerning whole range quality control of disposable sterile medical appliance from Oct 2002 to Oct 2005 in our hospital to summarize the experiences in this field. RESULTS During the last 3 years, the supply room in our hospital had administrated disposable sterile medical appliance 30 069 000 articles altogether, with the qualification ratio of 100%, and no adverse reaction was found; furthermore, the waste was reduced for no massed articles occurred; the cost accounting was raised and the error ratio during delivery was reduced too, the annual error ratio dropped form 0.003% to 0.00066%. CONCLUSIONS The whole range quality control of disposable sterile medical appliance can raise the administration quality, reduce the waste during delivery, and guarantee the disposable sterile medical appliance being used safely and effectively, also, it can provide accurate and useful data of cost accounting to concerned departments.

Objective To localize the gene of a Chinese autosomal-dominant non-syndrome deafness (DFNA) family by linkage analysis. Methods Pedigree was drawn after genetic investigation. All family members were checked up, and detail audiology examination proceeded (including pure tone audiometer, acoustic immittance measurement, auditory brainstem response). Vestibular function was evaluated. At first, the similarity of the family's locus linkage with the known DFNA loci should be excluded, and then whole genome scan was carried out. Results Deafness gene of this family was localized at 2q13-q14.2. A maximum LOD score of 3.22 (?=0.00) was obtained with the microsatellite marker D2S363. Haplotype analysis placed the novel locus within a 8.4cM region interval defined by markers D2S1888 and 2S2224. DNA sequencing of coding regions and exon/intron boundaries of a candidate gene PAX8 in this interval did not reveal disease-causing mutation in this family. Conclusion A novel DFNA locus was defined in a Chinese Neimeng family.