Recent advances in carbohydrate metabolism in cancer stem cells were obtained by analyzing the key words in relation with carbohydrate metabolism in cancer stem cells, which are covered in cluster-derived databases ( GoPubMed, XplorMed, AnneO'Tate, MEDSUM, PubReMiner) , with the basic strategies and use characteristics of these 5 databases compared, in order to provide the effective retrieval tools for embedded subject service in li-brary.

Objective:To observe the postoperative complications of percutaneous coronary intervention (PCI)in pa-tients With coronary heart disease (CHD)and analyze its nursing methed.Methods:A total of 200 CHD patients un-dergoing PCI in our hospital from Sep 2011 to Aug 2012 Were enrolled.The postoperative complications and thera-peutic effect Were observed and analyzeits nursing methods.Results:There Were 42 cases of PCI postoperative com-plications,included 26 cases (13%)of forearm hematoma,five cases (2.5%)of errhysis and edema at puncture site,four cases (2%)of extubation syndrome,tWo cases (1%)of arrhythmia and tWo cases (1%)of pseudoaneu-rysm etc.,in Which the incidence of forearm hematoma Was the highest,compared With any other complication (P<0.05 );after direction nursing,all complications significantly improved and recovered to normal.Conclusion:Comprehensive and directive nursing can effectively prevent postoperative complications and promote rehabilitation as soon as possible in patients With coronary heart disease after percutaneous coronary intervention.

Objective:To observe the effects of Periploca forrestil Schltr Compound extract in serum interleukin-4(IL-4),interferon-γ(IFN-γ),tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) in joint synovial tissue of rats,and further to investigate it′s therapeutic effects and related mechanism.Methods: To built the complete adjuvant arthritis (AA) model in rats,and then to detect the contents of IL-4,IFN-γ,TNF-α in serum by ELISA,TGF-β1 in synovial tissues of rats knee was detected by immunohistochemistry.Results: The contents of IFN-γ,TNF-α were higher and IL-4 and TGF-β1 were lower in adjuvant arthritis rats (model group) than those in blank control (P<0.01).By comparing with the model group the contents of IFN-γ and TNF-α were significantly lower in Periploca forrestil Schltr Compound treated rat group,whereas the expressions of IL-4 and TGF-β1 were increased(P<0.05).Conclusion: The Periploca forrestil Schltr Compound can significantly inhibit the expression of IFN-γ and TNF-α,and enhance the expression of IL-4 and TGF-β1,adjusted the Th1/Th2 cell and cytokines imbalance,to alleviate arthritis and cartilage destruction,therefore the Periploca forrestil Schltr Compound can significantly inhibit the proliferation of T lymphocyte and infiltration in synovial tissues of experimental rheumatoid arthritis.

Cancer is a general term of a series of complex traits of the disease triggered by the body cells losing their normal regulation of excessive proliferation, which essentially is a genetic disease.Recombinant inbred strain (RI) mouse generated from one pair of founders has been widely used in traditional tumor model.However, RI has many limitations on the statistic efficiency because of the small scale and lacking of allele diversity.The Collaborative Cross (CC) was designed to generate hundreds of recombinant inbred lines by 8 divergent strains of mice.CC mice embody a tremendous amount of natural genetic variation in different sub-strains of mouse and the single nucleotide polymorphism is four times of the traditional experimental mice.The high-genetic diversity and large scale population enables CC mice simulates the differences of individual susceptibility to the pathogeny or the therapies,thus provides a better research tool and information platform for expediting discovery of genes and genes function in human complex traits diseases.This review summarizes our current knowledge of this field, including methodologic aspects, applications, challenges and limitations, and utilization for cancer research.

In order to accelerate the comprehensive reform of clinical education,the Ministry of Education and the Ministry of Health decided to implement the program of excellent doctor education training plan in 2012.On the basis of the plan,taking a certain medical college as an example,on the problems such as lack of teachers,unreasonable age structure,the lack of advanced experimental technology and comprehensive talents and the lack of innovation and enterprising spirit and so on,the author puts forward some proposals from the development of experimental teaching,the teachers' personal and professional growth and the institutional and policy level,such as strengthening the foreign exchange,making learning training plan and personal development planning,establishing the evaluation system of the experiment teaching quality and opening up a variety of learning methods to improve the teachers' comprehensive ability.

Exudative erythoma multiforme is a related to the immune function of children with skin and mucosa,damage to its diversity of characteristics of acute non suppurative inflammation,one of the aller-gic diseases.The disease and its pathogenesis is complex,the body can cause multi system and multi organ damage,the cause has not yet entirely clear.In recent years,due to our understanding of the exudative erytho-ma multiforme gradually thorough,both domestic and abroad on the level of diagnosis and treatment of the disease have greatly improved,the prognosis of patients with exudative erythoma multiforme has improved significantly.The progress of the pathology,clinical manifestation,diagnosis and treatment of exudative ery-thoma multiforme were summarized.

Objective To analyze effect of stem cell transplantation on the expression of smooth muscle actin (SMA), basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) in the myocardium of dogs with ischemic mitral regurgitation.Methods 24 healthy male experiment dogs were selected, 6 were collected randondy as normal group, the others were used to construct mitral regurgitation model.After that, the modelized dogs were divided into three groups, 5 dogs in model group, 6 dogs in experiment group and 6 in control group.The experimental group were given 2 mL stem cells suspension to ischemic myocardial, control group was infused without stem cells in DMEM medium, and then the SMA, bFGF and VEGF expression levels of myocardial tissues in each groups were detected and analyzed.ResuIts Compared with control group and model group, the heart function of experiment group were improved more obviously, such as cardiac output (CO) and stroke volume (SV) increased significantly (P<0.05).Myocardialα-SMA were significantly lower (P<0.05).bFGF contents in myocardial tissue increased significantly (P<0.05).The content of VEGF in myocardial tissue significantly increased (P<0.05).ConcIusions Stem cell transplantation can inhibit the expression of SMA in myocardium of ischemic mitral regurgitation, and promote the expression of bFGF and VEGF, has a better effect on heart function.

Objective To test the utility of a new type of inflatable rehabilitation boot in the treatment of Danis-Weber type-A stable external malleolus fractures. Methods Fifty-four patients with stable external malleolus fractures were randomized into two groups. After closed reduction, the observation group was treated by immobilisation with the inflatable rehabilitation boot and the control group with a plaster slab. All were instructed to avoid weight-bearing on the affected side. The swelling volume of the injured limb was measured by drainage measurement before treatment and at the 1st, 3rd and 7th days after treatment. Pain was evaluated using a visual analogue scale (VAS) score before treatment and after 12, 24 and 72 hours. All of the ankles were x-rayed to recheck for displacement after 2weeks. Results The average volume of the injured limbs in the observation group was significantly less than in the control group at every time point. The average VAS score in the observation group was significantly lower than that in the control group. Fracture displacement in the observation group was also significantly better. Conclusions The inflatable rehabilitation boot has better curative efficacy than the typical plaster slab for patients with stable external malleolus fractures in terms of improving edema in the injured limb, relieving pain and preventing fracture displacement.

OBJECTIVE: To investigate the protective effect of Zingiber officinale extract on retinal ischemia-reperfusion (RIR) injury in rats. METHODS: 45 SD rats were randomly divided into normal control group, model group and Z.officinale extract group (400 mg?kg-1).RIR models were induced by infusing normal saline into anterior chambers for l hour. During five days before modeling,Z.officinale extract group were oral administration of Z.officinale extract two times per day by i.g. and model group distilled water. The medication regime remained after modeling. The activity of SOD and the content of malonyldialdehyde(MDA) and NO in retina were measured at 12 h,24 h,48 h and 72 h after modeling. The retinal ultrastructural changes were detected under the electron microscope. RESULTS: Compared with model group, in high-dose of Z.officinale extract group Z.officinale extract could strengthen the activity of SOD, reduce the levels of MDA and NO(P

Objective To investigate the expressions of survivin and bcl-2 and their relationships in transitional cell carcinoma of the bladder.Methods The expressions of survivin and bcl-2 in 40 cases of transitional cell carcinoma of the bladder were detected with streptavidin-biotin peroxidase(SP) method,with 15 cases of normal bladder specimens as controls.Results Survivin was expressed in 25 of 40(58.149%) cases of bladder carcinoma tissues,and normal bladder specimens did not express survivin(P