Objecitve To observe the relationship between the expressions of the estrogen receptor (ER),progestogen receptor (PR) and tumor infiltration and metastasis in thyroid cancer.Methods By using immunohistochemical method to study the relationship between the expressions of ER and PR in 100 cases of thyroid carcinamas and 28 cases of benign thyroid lesions.Results The positive rates of ER and RP expression were 67 0% and 62 0% respectively in thyroid carcinomas,it had correlation with well differentiation and type of histology but positive expression did not relate to age and sex.The positive rates of ER and PR in the non-metastasized group were 75 4% and 70 5% respectively;significantly higher than that of the metastasized group which were 53 8% and 48 7% respectively (P

Aminoacyl-tRNA synthetase catalyzing the first reaction of protein biosynthesis. In mammalian cells, eight aminoacyl-tRNA synthetases (aaRSs) and three auxiliary protein factors form a macromolecular aminoacyl-tRNA synthetases complex (aaRS complex). The three nonsynthetase protein factors, namely, p43, p38, and p18 were found to be involved in many other important life activities besides their roles in the complex. The auxiliary factor p43 was the precursor of endothelial monocyte activating polypeptideⅡ (EMAPⅡ), which involved in angiogenesis and apoptosis. The auxiliary factor p38 was crucial for the development of lung, and its abnormal accumulation in neuron would be related to the Parkinson’s disease. The auxiliary factor p38 and p18 could promote the repair of DNA damage via different pathways in a highly organized way. All these breakthroughs enhance our understanding about the interaction between the aaRS complex and the macromolecular signaling network and promote the studies on this field.

Objective To evaluate the quality of examination papers of otolaryngology among students of grade 2007 in the first clinical college of Chongqing Medical University.Methods Totally 202 copies of examination papers of otolaryngology from grade 2007 students in our college were taken as research references.SASS 15.0 software was used to statistically analyze total score of every student and score of each question.Mean score,standard deviation,difficulty coefficient and discrimination coefficient of each question were counted and normality test was conducted.Results Scores were in negative skewness distribution,with mean score of 80.9,standard deviation of 10.9,the lowest score of 56.5,the highest score of 98.0,difficulty coefficient of 0.781,and discrimination coefficient of 0.308.Conclusions Examination papers are well designed and discriminated and can objectively reflect students' command of the course.However,distribution of sores is unreasonable and examination paper is relatively easy to complete,indicating the weakness in the teaching process.

Objective To analyze the inducement,clinical manifestations,diagnosis and treatment of diabetes patients with multiple organ failure(MOF).Methods A retrospective analysis of 64 diabetes patients with multiple organ failure was executed,the clinical data were statistically analyzed.Results Heart failure was the most common in 64 cases with a incidence of 100.0％,the rest common failure organ were kidney(92.2％ ),brain( 32.8％ ),lung ( 14.1％ ),gastrointestinal tract (3.1％ ),and liver( 1.6％ ).Cases with the glucose level over 33.5mmol/L illustrated a significantly higher incidence than other groups ( P ＜ 0.05 ),glucose level between 22.5 and 33.4mmol/L group was significantly higher than the 12.2 ～ 22.4mmol/L group( P ＜ 0.05 ) The inducement of MOF were infection (76.6％ ) medication terminate ( 9.4％ ),ketoacidosis ( 7.8％ ),cardiovascular and cerebrovascular accidents (6.3％ ).Conclusion Positive control of blood glucose in the early stage of diabetes,prevent complications,eliminate the incentive could reduce the incidence of MOF,eventually reduce the mortality rate.

Objective To evaluate the value of the machine-drawn time-intensity curves with intravenous contrast-enhanced color Doppler imaging in study of perfusion of normal kidneys of the rabbits.Methods A series of sequent pictures of 16 normal kidneys of 8 rabbits with color Doppler flow imaging(CDFI) and color Doppler power imaging(CDPI) were collected,and time-intensity curves were drawn by the ultrasound instrument after intravenous administration of "Quanfuxian" contrast medium.Quantitative analyses were done by moving the "interest line".Results The time-intensity curves showed as single apex with steep ascending and slowly descending branch after contrast enhancement.Conclusions The machine-drawn time-intensity curves with intravenous contrast-enhanced color Doppler imaging could efficiently show the perfusion of kidney.

To investigate the changs in the expressions of cellular adhesion molecules (CAMs) of the myocardium after low dose interleukin 1? (IL 1?) pretreatment and the relationship between CAMs and delayed cardioprotection, we measured the change in the expressions of intercellular adhesion molecule 1 (ICAM 1) mRNA and its proteins, the expressions of leukocyte function antigen 1(CD11a) proteins, and infiltration number of polymorphonuclear leukocytes (PMNs) in the myocardium and determined the infarct size with in situ hybridization(ISH),immunohistochemistry and enzyme methods immediately,12 hours and 24 hours after IL 1? pretreatment in a model of ischemia/reperfusion of myocardium in rats. The results showed that the expressions of ICAM 1 mRNA and its protein, CD11a protein and the PMN infiltration number were significantly higher in the ILPC and NS groups than in the control at 0～24h after IL 1? or NS pretreatment( P 0 05). The results suggested that low dose IL 1? pretreatment might inhibit the expressions in the CAMs of myocardium during the late period of ischemia /reperfusion,resulting in delayed cardioprotection.

Objective To explore the clinical application value of combined detection of serum brain natriuretic peptide (BNP),homocysteine(HCY) and blood lipids in the patients with heart failure.Methods The levels of serum BNP,HCY,TC,TG,HDL-C,LDL-C,ApoA,ApoB and Lpa were detected in 100 patients with heart failure (observation group) and contemporaneous 100 persons (control group) undergoing healthy physical examination.Then the comparative analysis was performed.Results Compared with the control group,the BNP and HCY levels in the observation group were significantly increased(P＜0.05);among 7 indicators of the blood lipid,the Lpa level in the observation group was increased compared with the control group,while the TC,HDL-C and LDL-C levels were significantly decreased(P＜0.05),and the TG,ApoA and ApoB levels had no statistical differences between the two groups(P＞0.05).Conclusion Serum BNP,HCY,TC,HDL-C,LDL-C and Lpa levels have close correlation with heart failure,especially the combined detection of BNP,HCY and Lpa,which has predictive and diagnostic value in heart failure and is worthy of clinical popularization and application.

AIM:To study incorporate changes of compound in safflower infusion solution before and after heating by HPLC fingerprint, in order to provide reference of drug’s safety evaluation. METHODS: Hydroxysafflor yellow A was used as reference substance, safflower infusion solution was detected by HPLC fingerprint in the whole manufacturing process from original raw material、intermediate to finished product.The detector temperature was set at room temperature、100 ℃、115 ℃,and then the change in the peak’ disappearance and appearance was observed and the peak area’ decrease and increase showed by characteristic absorption peak of every sample product at different temperature. RESULTS: As the temperature rose to 105 ℃,new peaks appeared at the retention time of 7、26-27、44 minutes,original peaks would disappear at the time 8、36minutes.As the temperature continued to rise to 115 ℃,the peak at the time of 7、10 minutes disappeared again.the peak area increased during the temperature rising at retention the time of 31-32、44 minutes and decreased at the retention time of 37 minutes. CONCLUSION: It indicates that,heating safflower infusion solution would produce a new compound. As the temperature continues to rise,the new compound would be destroyed.

Objective To study the characters of using the high pressure injector in the contrast CT scanning for children. Methods Summarized the nursing measures of 407 children who had accepted the contrast CT scanning. Results There were 369 children have obtained perfect effects of CT scanning, 35 children have obtained general effects and there were 2 children failure. Conclusion The method of using high pressure injector in the contrast CT scanning for children is convenient, safety and effective.

Objective To compare internet addiction disorders' family cohesion and their family educa-tion style, and to help prevent internet addiction disorder and interference treatment in families with internet addic-tion disorder. Methods Ninety adolescents with internet addiction disorder were investigated as the experimental group and one hundred normal adolescents as the control group. Questionnaire and in-depth interview was used to collect data,and the data was analyzed with SPSS 10.0. Results Average scores of control group were significant higher than those of experimental group in family cohesion ((51.75±8.86) vs (38.89±12.17), P<0.01). Av-erage scores of control group were significant higher than these of experimental group in family education style ((50.65±7.66) vs (41.44±10.84), P < 0.01). Average scores of experimental group were significant higher than those of control group in dissatisfaction of family cohesion and family education style ((20.87±4.66) vs (12.33±7.88); (23.30±11.06) vs (14.28±7.29), P < 0.01). Conclusion The adolescents with internet addiction disorder have low family cohesion and high dissatisfaction of family education style,wherefore during in-ternet addiction family intervention and prevention, it should pay particular attention to cultivating close and harmo-nious relations among families members. In aspect of family education, parents should pay special attention to chil-dren's healthy personality development, and guide their children to actively take part in social practice and train-ing.