Cellular replicative senescence is a major contributing factor to aging and to the development and progression of aging-associated diseases. In this study, we sought to determine viral replication efficiency of influenza virus (IFV) and Varicella Zoster Virus (VZV) infection in senescent cells. Primary human bronchial epithelial cells (HBE) or human dermal fibroblasts (HDF) were allowed to undergo numbers of passages to induce replicative senescence. Induction of replicative senescence in cells was validated by positive senescence-associated β-galactosidase staining. Increased susceptibility to both IFV and VZV infection was observed in senescent HBE and HDF cells, respectively, resulting in higher numbers of plaque formation, along with the upregulation of major viral antigen expression than that in the non-senescent cells. Interestingly, mRNA fold induction level of virus-induced type I interferon (IFN) was attenuated by senescence, whereas IFN-mediated antiviral effect remained robust and potent in virus-infected senescent cells. Additionally, we show that a longevity-promoting gene, sirtuin 1 (SIRT1), has antiviral role against influenza virus infection. In conclusion, our data indicate that enhanced viral replication by cellular senescence could be due to senescence-mediated reduction of virus-induced type I IFN expression.
Aging ; Cell Aging* ; Epithelial Cells ; Fibroblasts ; Herpesvirus 3, Human ; Humans ; Influenza, Human ; Interferon Type I ; Orthomyxoviridae ; RNA, Messenger ; Sirtuin 1 ; Up-Regulation

OBJECTIVE: Cerebral hyperperfusion syndrome after extracranial-intracranial(EC-IC) bypass is a rare event but it may be disastrous. It can cause vomiting, confusion, seizure and intracerebral hemorrhage. We report 11 cases of hyperperfusion syndrome after EC-IC bypass surgery for recent 5 years in detail. METHODS: Authors performed EC-IC bypass surgery for augmentation of cerebral blood flow in 60 patients for recent 5 years. In 60 patients of EC-IC bypass procedures, we experienced 11 cases of hyperperfusion syndrome. It was observed that hyperfusion induced headache and vomiting in 2 cases, seizure in 1 case, temporary neurologic deficit in 5 cases, and intracerebral hemorrhage in 3 cases. RESULTS: All patients except three cases of intracerebral hemorrhag recovered completely. Five patients with temporary neurologic deficit improved within maxmum of 10days. In three cases of intracerebral hemorrhage, emergency hematoma removal was performed. Two of them, with moyamoya disease, recovered with minor neurologic deficit. The other, with traumatic intracarotid artery injury died. CONCLUSION: Hyperfusion syndromes may be due to relative hyperperfusion of a cerebral hemisphere in which autoregulation had been impaired because of preoperative chronic hypoperfusion. We strongly recommend that revascularization is deferred to 8 weeks till impaired autoregulation is restored, and meticulous blood pressure control should be done in the postoperative course of EC-IC bypass surgery.
Arteries ; Blood Pressure ; Cerebral Hemorrhage ; Cerebrum ; Emergencies ; Headache ; Hematoma ; Homeostasis ; Humans ; Moyamoya Disease ; Neurologic Manifestations ; Seizures ; Vomiting

OBJECTIVE: The purpose of this study is to ascertain whether magnetic resonance(MR) images taken after ethanol injection or microwave irradiation into feline brain can verify changes within the brain and offer valuable information about the spatial extent of the induced lesion. METHODS: In the ethanol injection experiment, nine male cats were divided into three groups including group I(n =3) treated with 0.1cc ethanol injection, group II(n=3) 0.2cc ethanol, and group III(n=3) 0.3cc ethanol into the feline brains. In the microwave irradiation experiment, twelve male cats were divided into four groups including group I(n=3) irradiated with 30 watt electrical power with 10 sec duration, group II(n=3) same power with 30 sec duration, group III(n=3) irradiated with 60 watt power with 10 sec duration, and group IV irradiated with 60 watt power with 30 sec duration. MR images were obtained in both ethanol injection and microwave irradiation experiments. Pathologic examinations were done after completion of MR imagings. RESULTS: Contrast-enhanced T1-weighted MR images showing nodular or rim enhancement were most reliable in delineating the extent of the necrosis induced by ethanol injection and microwave irradiation. The contrast enhancement corresponded with gliosis in normal brain surrounding the necrotic area and hypervascularity in ongoing necrotic area and adjacent normal brain. There were various enhancement patterns after ethanol injection with reflux of ethanol. In case of microwave irradiation, round or oval enhancements were shown with clear margin. The size of the enhancement was well correlated with the amount of injected ethanol and the amount of electrical power. Application time was not correlated with the size of enhancement in high electrical power group. The feature of the edema after ethanol injection was unpredictable and predictable in case of microwave irradiation. CONCLUSION: It is easy to predict the result in case of microwave irradiation, and the achieved results can be used as basic information in performing these procedures.
Animals ; Brain* ; Cats ; Edema ; Ethanol* ; Gliosis ; Humans ; Magnetic Resonance Imaging* ; Male ; Microwaves* ; Necrosis

BACKGROUND/OBJECTIVES: Oligonol, mainly found in lychee fruit, is an antioxidant polyphenolic compound which has been shown to have anti-inflammatory and anti-cancer properties. The detailed mechanisms by which oligonol may act as an anti-aging molecule have not been determined. MATERIALS/METHODS: In this study, we evaluated the ability of oligonol to modulate sirtuin (SIRT) expression in human lung epithelial (A549) cells. Oligonol was added to A549 cells and reactive oxygen species production, mitochondrial superoxide formation, and p21 protein levels were measured. Signaling pathways activated upon oligonol treatment were also determined by western blotting. Furthermore, the anti-aging effect of oligonol was evaluated ex vivo in mouse splenocytes and in vivo in Caenorhabditis elegans. RESULTS: Oligonol specifically induced the expression of SIRT1, whose activity is linked to gene expression, metabolic control, and healthy aging. In response to influenza virus infection of A549 cells, oligonol treatment significantly up-regulated SIRT1 expression and down-regulated viral hemagglutinin expression. Oligonol treatment also resulted in the activation of autophagy pathways and the phosphorylation of AMP-activated protein kinase (AMPK). Furthermore, oligonol-treated spleen lymphocytes from old mice showed increased cell proliferation, and mRNA levels of SIRT1 in the lungs of old mice were significantly lower than those in the lungs of young mice. Additionally, in vivo lethality assay revealed that oligonol extended the lifespan of C. elegans infected with lethal Vibrio cholerae. CONCLUSIONS: These data demonstrated that oligonol may act as an anti-aging molecule by modulating SIRT1/autophagy/AMPK pathways.
Aging ; AMP-Activated Protein Kinases ; Animals ; Autophagy ; Blotting, Western ; Caenorhabditis elegans ; Cell Proliferation ; Fruit ; Gene Expression ; Hemagglutinins, Viral ; Humans ; Litchi ; Lung ; Lymphocytes ; Mice ; Orthomyxoviridae ; Phosphorylation ; Reactive Oxygen Species ; RNA, Messenger ; Spleen ; Superoxides ; Vibrio cholerae

The aim of this project was to use 3D scanning data collected at incident scenes and various evidence to 1) develop surveying methods based on 3D data consisting of overall and detailed scene evidence, captured by long-range and micros-canner, which can be shared by personnel working in different fields such as forensic medicine, video analysis, physical analysis, traffic engineering, and fire investigation; 2) create digital storage for human skeletons and set the foundation for virtual anthropology; and 3) improve the credibility of 3D evidence by virtual remodeling and simulation of incident scenes and evidence to provide a basis for advanced and high-tech scientific investigation. Two complete skeletons of male and female were scanned using 3D micro-scanner. Each bone was successfully reproduced and assembled in virtual space. In addition, recreating evidence scheduled for invasive examination by creating RP (rapid prototype) was possible. These outcomes could play an important role in setting up the new field of virtual anthropology. Case-specific surveying methods were developed through analysis of 3D scanning data collected by long-range surface scanners at the scenes of vehicular accidents, falls, shootings, and violent crimes. A technique and recording method was also developed for detecting forged seals by micro-scanning the pressure exerted on the seal. Appraisal methods developed in this project could be utilized to secure 3D data of human skeletal remains and incident scenes, create a standard for application, and increase objectivity, reproducibility, and accuracy of scanning methods. We plan to develop case-specific 3D data analysis techniques to improve the credibility of analysis at the NFS and to establish a 3D data collection and analysis team.
Crime ; Data Collection ; Female ; Fires ; Forensic Medicine ; Forensic Sciences ; Humans ; Male ; Skeleton ; Statistics as Topic