Liver biopsies are used routinely in the assessment of graft dysfunction following liver transplantation and generally considered to be the most reliable method for the diagnosis of posttransplant complications with overlapping clinical and laboratory findings. To investigate posttransplant complications causing graft dysfunction and usefulness of liver biopsy, we analysed clinicopathologic features of 65 posttransplant liver biopsies, 2 autopsies and an explanted liver, taken from 20 patients. The frequencies of posttransplant complications were acute cellular rejection in 9 patients (45%), postoperative infection in 11 patients (55%), of which cytomegalovirus (CMV) infection and systemic invasive aspergillosis with candidiasis occured in 10 patients (50%) and 1 patient (5%), respectively. Remainders were hepatic arterial thrombosis in two (10%), primary graft dysfunction due to fatty donor liver in one (5%), and posttransplant lymphoproliferative disorder (PTLD) in two (10%). There were no chronic rejection or recurrent disease. Postoperative mortality was 25%. Histologic grade by Banff schema was well correlated with clinical parameters associated with unfavorable short term prognosis. CMV infection was associated with acute cellular rejection in 6 out of 10 patients (60%). Immunohistochemical staining for CMV was more sensitive method than CMV in situ hybridization or histologic detection of viral inclusion on tissue section. It was unique that one case of PTLD developed under the circumstances of the lowest dosage of immunosuppression and took grave outcome. Based on these results, we concluded that clinicopathologic correlation with integration of all the clinical and laboratory findings is necessary in the interpretation of accurate and early diagnosis of posttransplant liver biopsies. The interrelationship between chronic rejection and CMV infection as well as pathogenetic factors of PTLD remains to be clarified through further ongoing observation.
Aspergillosis ; Autopsy ; Biopsy* ; Candidiasis ; Cytomegalovirus ; Diagnosis ; Early Diagnosis ; Humans ; Immunosuppression ; In Situ Hybridization ; Liver Transplantation ; Liver* ; Lymphoproliferative Disorders ; Mortality ; Primary Graft Dysfunction ; Prognosis ; Thrombosis ; Tissue Donors ; Transplants

No abstract available.
Ascites* ; Gallbladder* ; Ultrasonography*

No abstract available.

No abstract available.
Adenocarcinoma* ; Appendix*

Autophagy is an evolutionarily conserved catalytic process by which cytoplasmic components including damaged macromolecules and organelles are degraded. The role of autophagy includes adaptive responses to nutrition deprivation or intracellular stimuli. Although autophagosomes were first observed in early 1960s, it was 1990s that autophagy-related genes in yeast were identified and studied. Nowadays, the molecular machinery of autophagy and signaling pathway to various stimuli are almost outlined. Dysregulation of autophagic activity has been implicated in many human diseases including neurodegenerative diseases, infection and inflammation, and malignancies. However, since current understanding of autophagy in placenta is just at the beginning, this paper aims to provide general information on autophagy (part I) and to summarize articles on autophagy in human placenta (part II). This review article will serve as a basis for further researches on autophagy in relation to human pregnancy and its complications.
Autophagy* ; Cytoplasm ; Humans ; Inflammation ; Neurodegenerative Diseases ; Organelles ; Placenta* ; Pregnancy ; Trophoblasts ; Yeasts

Methanol and/or boiling water extraction of 201 natural products and subsequent MTT assay using MT-4 cell line was carried out to screen the anti-HIV-1 activity. Among 97 methanol extracts, 7 extracts from Chrysanthemi Indicium Flos, Magnoliae Cortex Machili Cortex, Reynoutriae Rhizoma, Lithospermi Radix Agastachis Herba, and Chaenomelis Fructus showed anti-HIV-1 activity and their SI value were 2.25 to 5.77. In addition, among 119 boiling water extracts, 10 extracts from Lonicerae Caulis et Foloium, Elsholtziae Herba, Leonuri Herba, Portulacae Herba, Schizonepetae Herba, Curcumae Rhizoma, Amomi Cardamomi Fructus, Cirsii Radix et Herba, Carpesii Herba, and Siegesbeckiae Herba showed anti-HIV-1 activity and their SI value were 1.30 to 7.64. Methanol extracts of above seven natural products were fractionated and the anti-HRs_1 activity of each fraction was examined. Extraction was carried out with hexane, chloroform, butanol, and water to trace active anti-HIV-1 componets. As a result, the water fraction of Magnoliae Cortex, Machili Cortex, Reynoutriae Rhizoma, Agastachis Herba, Chaenomelis Fructus and the butanol fraction of Chrysanthemi Indicium Flos, Reynoutriae Rhizoma showed anti-HIV-1 activity and their SI value were 1.40 to 8.02. We could reach a conclusion that studies to trace the anti-HIV-1 active component of each natural products in further Sractionation and to identify its structure by Infrared spectroscopy, NMR spectroscopy and gel permeation chromatography were needed.
Biological Products ; Cell Line ; Chloroform ; Chromatography, Gel ; Curcuma ; Lamiaceae ; Lithospermum ; Lonicera ; Magnetic Resonance Spectroscopy ; Magnolia ; Mass Screening* ; Methanol ; Portulaca ; Spectrum Analysis ; Water

PURPOSE: While the rate of breast-feeding has been rising recently in many countries, it has been gradually falling in Korea. Accordingly, we took a survey in Jeon-ju to discover the actual state of breast-feeding and find a solution. METHODS: Eight hundred and sixty-four questionnaires of filled out by mothers who visited the seven local pediatric clinics and the clinic of pediatric department in the Presbyterian Medical Center(PMC) in Jeon-ju from January 1998 to June 1998 were analysed by ANOVA, cross analysis, frequency analysis and correlation analysis using SPSS program. RESULTS: In the case of six-months-old babies, the breast-feeding rate was 35.6%. The breast-feeding rate was higher when it came to a normal delivery, and was also related to the inperiority of infant birth order and lack of maternal academic background(P<0.05). The main reasons to switch from successful breast-feeding to formula-feeding before 6 months of age were insufficient breast-milk(49.3%). The survey shows the best thing for a infant's health is breast milk (86.0%). CONCLUSION: In order to increase the rate of breast-feeding, it's essential to educate people in school and it is necessary to create a hospital environment in which mothers can easily begin breast-feeding as soon as possible after delivery.
Birth Order ; Humans ; Infant ; Jeollabuk-do* ; Korea ; Milk, Human ; Mothers ; Protestantism ; Surveys and Questionnaires

Although every pregnant woman and her physician hope for an easy pregnancy free of complications, complications can occur to a greater or lesser extent, some of which are still considered inevitable. The maternal mortality ratio in the Republic of Korea recently increased from 13.5 per 100,000 live births in 2009 to 17.2 in 2011, along with a noticeable increase, of up to 20%, in the proportion of older pregnant women (>35 years old). In contrast to postpartum bleeding, which has decreased, amniotic fluid embolism and pulmonary embolism, which are closely related to older maternal age and typically considered inevitable, are causing an increasing proportion of maternal mortalities. The neonatal mortality rate, defined the rate of death per 1,000 live births under 28 days of life, was reported to be 1.7 in 2011 in Korea and respiratory distress of newborns accounts for about one third of neonatal deaths. The pre-valence of cerebral palsy (CP) is approximately 2 per 1,000 live-born children and has remained unchanged over recent decades worldwide. Although multiple antenatal factors, including pre-term birth, low birth weight, infection/inflammation, multiple gestation, and other pregnancy com-plications have been frequently associated with CP, the underlying causes of CP remain largely unknown and recent evidence has indicated that birth asphyxia plays a minor role. This review provides information on the contemporary medical understanding of amniotic fluid embolism, pulmonary embolism, meconium aspiration syndrome, and CP, which are generally considered to be 'no-fault accidents' during birth.
Amniotic Fluid ; Asphyxia ; Cerebral Palsy ; Child ; Embolism ; Embolism, Amniotic Fluid ; Female ; Hemorrhage ; Humans ; Infant ; Infant Mortality ; Infant, Low Birth Weight ; Infant, Newborn ; Korea ; Live Birth ; Maternal Age ; Maternal Mortality ; Meconium ; Meconium Aspiration Syndrome ; Parturition ; Postpartum Period ; Pregnancy ; Pregnant Women ; Pulmonary Embolism ; Republic of Korea

The physical activity has an important physiological and psychological benefit for all people, and the exercise program has a key role in the management of diabetes. This article presents exercise recommendations for people with diabetes. Though the patients with diabetes may give many benefits from regular physical exercise, there may be several hazards from exercise as well. We assessed 30 patients with type 2 Diabetes Mellitus and 15 normal control subjects with sedentary life-style for their workload, heart rate on maximal performance and maximal oxygen uptake, change of blood glucose level after exercise using bicycle ergometer and Astrand nomogram. The workload, heart rate on maximal performance and maximal oxygen uptake were lower in diabetic patient than control subjects. Blood glucose decreased in diabetic patient than control subjects, after exercise and the lowered value was maintained until 60 minutes after exercise. The maximal oxygen uptake was lower in diabetic patients than control subjects. The blood glucose decreased in both diabetic patients and normal control after exercise.
Blood Glucose ; Diabetes Mellitus* ; Diabetes Mellitus, Type 2 ; Exercise ; Heart Rate ; Humans ; Motor Activity ; Nomograms ; Oxygen

PURPOSE: Zinc ion is critical for the functional and structural integrity of eukaryotic cells and participate in the regulation of apoptosis. In general, zinc inhibits a nuclear endonuclease, thereby causing inhibition of apoptosis. Recent studies have pointed to a role for a family of caspase proteases that act upstream of endonuclease. The widely used chemotherapeutic agents exert effects by inducing of apoptosis in sensitive tumor cells. In this study, we investigated the effects of zinc ion and other divalent cation on the idarubicin (IDA)-induced apoptosis of HL-60 cells. In addition, to determine whether Zn inhibits an event upstream of endonuclease activation, we analysed the activity of caspase-3, 9 and proteolytic cleavage of procaspase-3 and PARP [poly (ADP-ribose) polymerase]. METHODS: HL-60 cells were cultured in RPMI 1640 and treated with various doses and time periods of IDA with or without pretreatment of ZnCl2, CaCl2 and MgCl2. Cell viability was measured by trypan blue staining. For detection of apoptotic death, cells were stained with Hoechst dye and observed under fluorescence microscopy. The activities of caspase-3 and caspase-9 were measured by the proteolytic cleavages of Ac- DEVD-AMC and Ac-LEHD-AFC as flurogenic substrates, respectively. The proteolytic cleavages of procaspase-3 and PARP were analyzed by Western blotting using anti- caspase-3 and anti-PARP antibody, respectively. RESULTS: IDA induced the apoptotic death of HL-60 cells in a dose and time dependent manner, which was characterized by increasing chromatin condensation and DNA fragmentation. Pretreatment of HL-60 cells with ZnCl2 caused potent inhibition of IDA-induced apoptosis. Consistent with apoptotic death of HL-60 cells, IDA induced the catalytic activation of caspase-3 and caspase-9. After pretreatment of ZnCl2, the activation of caspase- 3 and the proteolysis of PARP induced by IDA were potently inhibited. But, after pretreatment of CaCl2 and MgCl2, there were no significant changes of IDA-induced apoptosis and proteases activity. CONCLUSION: Zinc ion suppressed the IDA-induced apoptosis via the inhibitions of caspase-9 and caspase-3. But calcium and magnesium ions didn't affect the IDA-induced apoptosis.
Apoptosis* ; Blotting, Western ; Calcium ; Caspase 3 ; Caspase 9 ; Cell Survival ; Chromatin ; DNA Fragmentation ; Eukaryotic Cells ; HL-60 Cells ; Humans ; Idarubicin ; Ions ; Magnesium ; Magnesium Chloride ; Microscopy, Fluorescence ; Peptide Hydrolases ; Proteolysis ; Trypan Blue ; Zinc